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Citations for Promega :
601 - 650 of 6151 citations for Cyclic GMP Direct Chemiluminescent ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... were sorted by FACS in 96-well V-bottom plate wells containing 2 μl of lysis buffer (Ultra-pure Water, 10% Triton X-100, RNasinPlus 40U/ul; Promega). After evaporation of the lysis buffer at 95°C for 3 min ...
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bioRxiv - Cancer Biology 2023Quote: ... in 6 well plates or 96 well plates (details in the figure legends) and assayed using crystal violet staining or Celltiter-Glo luminescent viability assay (Promega) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... The plates were incubated at 37°C for ~12 hours before luciferase activity was quantified using the Luciferase Assay System (Promega) using SoftMax Pro v.7.0.2 (Molecular Devices) ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of the lysate were transferred into two Microtiter plates (one for Renilla and the second for Bright Glo (Promega)) ...
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bioRxiv - Biochemistry 2023Quote: Approximately 0.1 million cells/well of a 12-well plate were seeded with HEK293T cell line and transfected after 70% confluency using a Lipofectamine reagent (Promega). For the cycloheximide assay ...
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bioRxiv - Cancer Biology 2023Quote: ... with 250 cells plated per well in 96-well flat-bottom plates in 100 μl medium and an equal volume of the CellTiter-Glo Luminescent Assay Reagent (#G7571; Promega) added at the indicated time-points ...
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bioRxiv - Microbiology 2023Quote: ... Quantification of OMV production in 96-well plate format was done by performing Nano-Glo assays using the Nano-Glo Live Cell Assay System (Promega). Briefly ...
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bioRxiv - Bioengineering 2023Quote: For luciferase expression 20 uL of undiluted sample was loaded onto a black 96 well-plate then 100uL luciferin solution (Promega) added immediately before reading on a luminometer (Wallac) ...
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bioRxiv - Bioengineering 2023Quote: ... The day after the plates were pre-cooled (RT, 15’) supplemented with luciferase substrate (Bright Glo Luciferase Assay System, Promega) incubated (RT ...
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bioRxiv - Immunology 2023Quote: ... The reaction was stopped by KPL TBM Stop Solution (SeraCare, #5150-0021) and plates were read at 450nm on GloMax Explorer Multimode Microplate Reader (Promega).
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bioRxiv - Bioengineering 2023Quote: ... The fluorescence intensity of each well and empty controls was measured at 520 nm excitation on a Glomax plate reader (Promega). The average blank control values for each plate were subtracted from individual fluorescence intensity values and weekly measurements were normalized to the initial reading.
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bioRxiv - Cell Biology 2022Quote: ... 5×104 cells/well were seeded in 24-well plates and transfected with 10 ng pRL-CMV (Promega, Walldorf, Germany) together with either 250 ng pSuper8xTOPFlash or 250 ng pSuper8xFOPFlash [22] using the FuGENE6® reagent (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: 500 cells/well were seeded in 96-well flat-bottom plates in 100 ml medium and an equal volume of the CellTiter-Glo Luminescent Assay Reagent (#G7571; Promega) added at the indicated time-points ...
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bioRxiv - Cancer Biology 2023Quote: ... the plates were allowed to equilibrate to room temperature for 30 minutes and 25 μL of Cytotox Glo solution (Promega) was added to the wells using a Wellmate plate filler ...
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bioRxiv - Microbiology 2023Quote: ... 8 × 105 HEK293T cells/well were seeded into 6-well plates and transfected the next day with ViaFect (Promega, E4981), using either 4 μg of plasmid expressing the G protein or 2 μg of plasmid expressing HA together with 2 μg of plasmid expressing NA ...
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bioRxiv - Microbiology 2023Quote: ... 50 μl were transferred into a white flat bottom 96 well plate in triplicates and 50 μl of Promega NanoGlo Luciferase assay reagent (Promega) prepared according to the manufacturer’s instructions was added to the wells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 5 × 104 transfected cells were replated into white 96-well tissue culture plates in the presence or absence of HaloTag NanoBRET 618 Ligand (Promega) and incubated overnight at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Amounts of protein binding to M.tb were quantified by colorimetric measurements by absorbance at 450 nm using a GloMax plate reader (Version 3.0; Promega Corporation), and plotted based on absorbance values relative to the baseline (negative control ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells was grown at 12-well plates for 18h and transfected with MCHR1 constructs and the cAMP biosensor GloSensor-22F (Promega) at a ratio of 3:2 ...
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bioRxiv - Cancer Biology 2023Quote: ... The following day 100 μl of each sample was transferred to a white 96-well plate and 100 μl of the the mix of Bright-GloTM Luciferase Assay (Promega) was added to each well ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 μL of the cell lysate was transferred into a white 96-well plate and 30 μL of firefly luciferase substrate (Promega) was added ...
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bioRxiv - Cell Biology 2023Quote: ... cells grown to ∼70% confluence in 6-well plates and co-transfected with a gRNA to TBC1D23 (12) and pmaxGFP using Fugene6 (Promega) according to manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... pH 11 and the fluorescence was measured at 360 nm (ex) / 450 nm (em) using GloMax Explorer plate reader (Promega). The heparan sulfatase activity was confirmed by our recently described 6-O desulfation assay using GlcNS6S-GlcA-Glc6SNS-IdoA2S-GlcNS6S-IdoA2S-GlcNS6S-GlcA-pNP (2S2-6S4 ...
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bioRxiv - Bioengineering 2023Quote: For luciferase expression 20 uL of undiluted sample was loaded onto a black 96 well-plate then 100uL luciferin solution (Promega) added immediately before reading on a luminometer (Wallac) ...
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bioRxiv - Microbiology 2024Quote: ... 4 μg of purified bacmid DNA were transfected into 1×106 Sf9 cells in a well of a 6-well plate using 6 μL of FuGENE HD transfection reagent (Promega). After 7 days of incubation at 27°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Wells containing single cell colonies were duplicated into 12-well plates and screened for HiBit expression by mixing crude lysates with purified LgBit protein (Promega) and measuring luminescence in the presence of 5 μM furimazine ...
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bioRxiv - Cancer Biology 2024Quote: ... were reverse transfected in a 96 well plate format with a custom siGENOME SMARTPool (Dharmacon) siRNA library as described previously72 using Lipofectamine RNAimax Transfection Reagent (Promega). Positive (siPLK1 ...
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bioRxiv - Molecular Biology 2024Quote: Cell viability assays were conducted in 96 well-plate format using Promega’s RealTime-Glo MT Cell Viability Assay (Promega G9712) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and luminescence from the activity of luciferase was measured at 48 h post-infection using a Tecan Infinite M200 Pro plate reader after applying Bright-Glo reagent (Promega) to the cells.
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bioRxiv - Biochemistry 2024Quote: ... NLuc expression was tested by luminescence measurement at 470 nm on the Envision plate reader using the Nano-Glo Luciferase Assay (Promega).
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bioRxiv - Microbiology 2024Quote: ... β-galactosidase activity was measured by absorbance at 420 nm in the Tecan Genios plate reader using 30 uL of each extract mixed with 30 uL of 2x Assay Buffer (Promega).
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bioRxiv - Biochemistry 2020Quote: ... GDP detection kit was from ProFoldin (MA, USA) and ADP-Glo™ Kinase Assay kit from Promega Corporation ...
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bioRxiv - Genetics 2022Quote: Genomic DNA was isolated from blood samples using commercial kit (Wizard® Genomic DNA Purification Kit; Promega), following the fabricant protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-β-Galactosidase (1:1.000; Promega, 1:1000; abcam), anti-Cleaved Caspase-3 (1:500 ...
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bioRxiv - Bioengineering 2020Quote: ... 1 µl random primers (50 ng·µl−1, Promega, C1181), 2 µL 0.1M DTT ...
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bioRxiv - Biochemistry 2024Quote: ... 7C (mouse, Promega cat# N700A; 1:500-1:1000); Nluc in Fig ...
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bioRxiv - Plant Biology 2021Quote: ... Genomic DNA was extracted using a DNA extraction kit (Wizard Genomic DNA Purification Kit; Promega Corp, Madison, WI). Genomic DNA was extracted from 1-week old bacterial sub cultures by collecting a loop of bacteria with a sterile pipette tip and inoculating a 0.2 ml PCR tube containing 15 μl of Molecular Grade Water and ran in a thermal cycler for 15 minutes at 95 °C.
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bioRxiv - Plant Biology 2023Quote: ... multiflorum samples using a commercial DNA extraction kit (Wizard HMW DNA Extraction Kit, Promega Corporation, Madison, WI, USA).
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bioRxiv - Cell Biology 2020Quote: ... Cell growth and viability were measured on the 96 well white bottom plate as an end point measurement for each time point and/or treatment using the Cell-titer GLO assay (Promega, # G7570) based on manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed with PBS and lysed directly on the plate on ice using the Cell Culture Lysis Reagent from the Luciferase Assay System (Promega#E1500). Lysates were transferred to microcentrifuge tubes ...
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bioRxiv - Neuroscience 2021Quote: ... neonatal and adult astrocytes plated on 96-well plates (50,000 cells per well) was measured with the Glucose Uptake-Glo™ Assay (Promega, J1341) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell viability was determined at 72 h post-seeding in 96-well round-bottom ULA cluster plates using CellTiter-Glo® (Promega). Western blot analysis was performed on adherent cell lysates at 72 h post-seeding to confirm DUSP4 knockdown.
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bioRxiv - Immunology 2021Quote: ... The indicated compounds were introduced analytically using a CyBio pintool and the plates left to incubate at 37 °C prior to the addition of the CellTiter-Glo reagent (Promega, G7573) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: 50 μl supernatant were collected as designed time point into a 96-well plate for lactate dehydrogenase (LDH) release assay as manufacture’s protocol (Promega, cat# G1780). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Crystal violet was the solubilized with a 75% ethanol solution and the absorbance was measured at a λ600nm with a plate reader (Promega GloMax Explorer, Promega, France).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were assayed in white 96 well plates in triplicate by the addition of 50 μl Bright-Glo™ Firefly luciferase assay system (Promega) or freshly prepared 40 ng/ml Coelenterazine in Renilla-Glo buffer (Promega) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 25 µL of the lysates were transferred to a white plate and mixed with the same volume of Nano-Glo® Luciferase reagent (Promega). For the A-MLV titers ...
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bioRxiv - Bioengineering 2021Quote: 100 μL of media was removed from each well on the plate and replaced with an equal amount of CellTiter-Glo reagent (Promega, G7572). Assayed plates were shaken at 500 RPM for 20 minutes and allowed to rest for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Media was refreshed on day 6 and plates collected on day 12 with cell viability quantified by CellTiter-Glo 3D (Promega #G9681). Dose response assay analysis was performed as described above.
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bioRxiv - Genomics 2022Quote: ... F-luciferase and R-luciferase expression was assayed in a TECAN Spark plate reader using the Dual-Glo® Luciferase Assay System (Promega) according to the manufacturer’s instructions ...