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Citations for Promega :
351 - 400 of 6859 citations for Cow G Protein Coupled Bile Acid Receptor 1 GPBAR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... an envelope plasmid encoding VSV-G and pAdVAntage (Promega). Four days after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... for the m.3243A>G and HapII (Promega #R6311) for the m.8993T>G and analysed by a 2% agarose gel with ethidium bromide ...
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bioRxiv - Molecular Biology 2021Quote: ... Human SMARCB1 was translated in vitro using TNT Quick Coupled Transcription/Translation System (L1170, Promega). 1 µg of pcDNA3.1-FLAG-SMARCB1 was incubated at 30°C for 90 minutes with 20 µM methionine and TNT T7 Quick Master Mix ...
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Membrane integration and topology of RIFIN and STEVOR proteins of the Plasmodium falciparum parasitebioRxiv - Biochemistry 2019Quote: ... Plasmid pGEM1 and TNT SP6 Quick Coupled Transcription/Translation System were purchased from Promega (USA). All enzymes were from ThermoFisher Scientific (USA ...
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bioRxiv - Cancer Biology 2019Quote: ... Immunoreactive bands were detected with horseradish peroxidase-coupled anti-mouse or anti-rabbit antibodies (Promega) using the ECL system (Merck Millipore WBKLS0500) ...
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bioRxiv - Biochemistry 2020Quote: ... Radiolabelled PDIP38 preprotein was synthesized using TNT® SP6 Quick Coupled Transcription-Translation System (Promega) with undigested pOTB7/PDIP38 as template and 11 μCi of [35S]Met/CysEXPRE35S35S protein labelling mix (specific activity of >1000 Ci/mmol ...
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bioRxiv - Cell Biology 2021Quote: ... which were each translated and transcribed in vitro with TNT-coupled reticulocyte lysate system (Promega) and imported for 10 min at 30°C in import buffer ...
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bioRxiv - Biochemistry 2022Quote: The Lep-derived constructs were assayed using the TNT T7 Quick Coupled System (#L1170, Promega). Each reaction containing 1 µL of PCR product ...
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bioRxiv - Molecular Biology 2022Quote: ... 25 and 63) were expressed using the TNT SP6 Coupled Reticulocyte Lysate System (Promega, L4600). Expression of Halo-bZIP and SBP-bZIP proteins were confirmed by western blotting using anti-HaloTag monoclonal antibody (Promega ...
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bioRxiv - Systems Biology 2023Quote: ... Halo-tagged TFs were expressed with TnT® Coupled Wheat Germ Extract System (Promega, USA) using 2 µg plasmid DNA ...
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bioRxiv - Physiology 2023Quote: Expression plasmids for luciferase and FincoR were mixed with a Coupled Reticulocyte Lysate System (Promega). After incubating at 30°C for 60 min ...
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bioRxiv - Biochemistry 2024Quote: ... an apo-TDO2 binding molecule (structure not disclosed) was coupled to the NanoBRET 590SE (Promega) dye to create a fluorescent tracer ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic acid was recovered using the Maxwell RSC instrument (Promega). After DNAse treatment and elution ...
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bioRxiv - Genomics 2019Quote: ... 35S labeled proteins were synthesized in vitro using the TNT T7 Quick for PCR DNA kit (Promega, L5540). Labeled proteins were incubated with MBP-tagged proteins in TNEN50 (50mM Tris pH7.5 ...
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bioRxiv - Microbiology 2021Quote: ATPase activity of the purified proteins was determined using two kits – Enliten ATPase assay (FF2000, Promega, Moscow, Russia) and malachite green phosphate assay (MAK307 ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were digested with 1 μg lysyl endopeptidase (LysC) (FUJIFILM Wako Pure Chemical Corporation) and 1 μg trypsin (Promega, Tokyo, Japan) overnight at 37°C on a shaking incubator ...
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bioRxiv - Immunology 2023Quote: Activation of chemokine receptors was detected by measuring ß-galactosidase activity using the commercially available Beta-Glo assay system (Promega Corp, Madison, WI, USA) and luminescence signal was recorded on a multimodal plate reader (Enspire 2300 ...
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bioRxiv - Molecular Biology 2023Quote: ... These cells were transfected in 70% confluent 6-wells TC-treated plates with 2 µg TGFβ receptor-Nanoluciferase constructs (constructs were provided by Promega and Promega R&D) using a 1 µg DNA ...
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bioRxiv - Systems Biology 2019Quote: Caspase 1 activity was measured using commercially available kit (Caspase 1 Glo inflammasome assay, Promega). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were digested by incubation with sequencing-grade modified trypsin (1/50 w/w; Promega, V5113) for 12 h at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Proteins were digested into peptides with 12μL of 1:10 Trypsin Gold (Promega, Madison, Wisconsin, V528A) in 50mM TEAB per sample ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were digested by incubation with sequencing-grade modified trypsin (1/50 w/w; Promega,V5113) for 12 h at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were digested for 6 h at 37°C with 4ng.μL−1 of modified trypsin (Promega) dissolved in 50 mM NH4CO3 ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were digested overnight at 37°C with 1 μg of mass spectrometry grade Trypsin (Promega). The resulting peptide samples were cleaned up for mass spectrometry in a Sep-Pak C18 column (Waters ...
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bioRxiv - Biochemistry 2022Quote: ... The protein from lysed cells were digested in 1 µL 10ng/µL of trypsin/LysC (Promega) in 100mM TEAB (ProtiFi) ...
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bioRxiv - Biophysics 2023Quote: ... the proteins were digested into tryptic peptides by incubation with 1□µg sequencing grade trypsin (Promega) overnight at 37□°C ...
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bioRxiv - Neuroscience 2023Quote: ... After the sequential digestion with LysC and trypsin (Promega, each protease to protein ratio 1:80), beads were retained with a magnetic rack and samples were filtered with Costar Spin-X spin filters (0·22 µm cut-off ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µg of reconstituted protein was digested in 0.05 N HCl with 1 µg pepsin (Promega) without prior reduction/alkylation at a volume of 200 µl 50 °C for 3 h ...
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bioRxiv - Cancer Biology 2024Quote: ... The identity of each cell line was confirmed by DNA fingerprinting via short tandem repeats at the time of mRNA and total protein lysate preparation using the PowerPlex 1.2 kit (Promega).
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bioRxiv - Molecular Biology 2020Quote: ... In vitro translation was performed using a TNT SP6 Quick Coupled Transcription / Translation System (Promega, L2080). 40 μl of rabbit reticulocyte lysates supplemented with methionine were incubated with 1 μg of pKS103-xLIG1 ...
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bioRxiv - Cell Biology 2020Quote: ... Horseradish peroxidase (HRP)-coupled secondary antibodies directed against mouse or rabbit IgG were purchased from Promega. Chemiluminescence images were acquired using the ImageQuant LAS 500 system (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: IVTT reactions were performed using the SP6 TnT® Quick Coupled Transcription/Translation System (Promega #L2080) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2019Quote: ... we used a control plasmid provided in the TnT® Quick Coupled Transcription/Translation System (Promega). The plasmid was linearized using the restriction enzyme SacI and purified using the DNA Clean and Concentrator Kit (Zymo Research) ...
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bioRxiv - Physiology 2022Quote: Synthesis of S35-labled CaVβ chimeras was done by coupled in vitro transcription-translation (Promega (L1170)) ...
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bioRxiv - Cell Biology 2022Quote: Sly1-HA3 was synthesized with the TNT® SP6 Quick Coupled Transcription/Translation system (#L2080, Promega), according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2023Quote: In-vitro translation assay was performed using TNT® Quick Coupled Transcription/Translation System (Promega #L1170) following the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2023Quote: ... Horseradish peroxidase (HRP)-coupled secondary antibodies directed against mouse or rabbit IgG were purchased from Promega. Chemiluminescence images were acquired using the ImageQuant LAS 500 or 600 system (GE Healthcare).
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Horseradish peroxidase (HRP)-coupled secondary antibodies directed against mouse or rabbit IgG were purchased from Promega for chemiluminescence imaging ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were then digested with LysC at 1ug/100ug protein (Promega) for 3 h at room temperature (RT ...
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bioRxiv - Plant Biology 2020Quote: ... Fusion proteins were purified using MagnetHis™ protein purification system (Promega) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: ... Proteins were precipitated through the addition of Protein Precipitation Solution (Promega) followed by vigorous vortexing ...
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bioRxiv - Neuroscience 2021Quote: ... ELISA was performed on the homogenate using the BDNF Emax Immuno Assay Systems (Promega KK, Tokyo, Japan). The hippocampus (HPC ...
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bioRxiv - Immunology 2023Quote: ... ELISA plates were immediately read using a GloMax®-Multi Detection System microplate reader (Promega, Madison WI) at 450 nm absorbance.
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bioRxiv - Cell Biology 2022Quote: ... were seeded into 24-well plate and cultured to 80% confluence when they were co-transfected with 4 μg of either TOP flash or FOP flash vector and 1 μg of pRL-TK (Renilla-TK-luciferase vector, Promega, Madison, WI, USA) using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... mixed with 1.5 g of guanidine thiocyanate (GuSCN, Promega V2791), vortexed to dissolve GuSCN ...
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bioRxiv - Neuroscience 2024Quote: ... VSV-G and Δ8.9 using FuGENE transfection reagent (Promega, E2311). 24 hours after transfection ...
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bioRxiv - Biochemistry 2021Quote: ... A 15-amino acid linker and SmBiT subunit (peptide 86, Promega) were attached to the C-terminus of rat Gβ1 ...
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bioRxiv - Microbiology 2022Quote: ... Nucleic acid extracts were treated using RQ1 RNAse-free DNAse (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... a complete standard mixture of amino acids (Promega, Medison, WI, USA) was analyzed by CE-MS to obtain MS and MS/MS spectra of the proteinogenic amino acids ...
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bioRxiv - Bioengineering 2023Quote: ... and subsequently stained with Diamond™ Nucleic Acid Dye (Promega, H1181) for 10 minutes ...