Labshake search
Citations for Promega :
251 - 300 of 6129 citations for Cortisone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... luminescence in each well was recorded using a GloMax Multi+ luminescence plate reader (Promega). For some experiments ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were then placed in a fluorescence-compatible plate reader (Promega, Madison, WI, USA) and fluorescence measured (GSH-bound Ex:475nm/Em:500-550nm ...
-
bioRxiv - Microbiology 2023Quote: ... luciferase was measured on a plate reader using the bright-glo luciferase reagent (Promega).
-
bioRxiv - Microbiology 2023Quote: ... Plates were stored at -20°C before adding 100 μl Steady Glo substrate (Promega) to perform a luciferase assay as described earlier ...
-
bioRxiv - Microbiology 2023Quote: ... and luciferase expression levels were assessed using a GloMax plate reader (Promega, Southampton, UK).
-
bioRxiv - Cancer Biology 2023Quote: ... Fluorescence intensity at 540ex/590em was read by the GloMax Discover plate reader (Promega). All experiments were repeated three times and one was shown.
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Plates were read for a period of 24 h on the GloMax Discover (Promega) set to 37 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Plates were collected at day 6 and measured by CellTiter-Glo (Promega #PR-G7573). Cell viability values were analyzed following blank cell deductions and normalization to vehicle readings ...
-
bioRxiv - Neuroscience 2023Quote: ... transferred to opaque 96-well plates containing 5 μM furimazine (NanoLuc Luciferase Assay, Promega), and bathed in 5-HT at various concentrations (ranging from 0.1 nM to 1 mM) ...
-
bioRxiv - Molecular Biology 2024Quote: ... After this colorization the plate was read using the Glomax multi microplate reader (Promega) at an absorbance wavelength of 560nm ...
-
bioRxiv - Bioengineering 2019Quote: ... Cultures were grown for 16 h as described above and then 1 mL of culture was used to isolate DNA using the Wizard DNA Purification Kit (Promega). A Nanodrop 2000 (Thermo Scientific ...
-
bioRxiv - Plant Biology 2020Quote: ... Nucleic acids deriving from PCRs or restriction digests reactions were purified from 1% TAE agarose gels with the “Wizard SV Gel and Purification System Kit” (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: A random-primers based reverse transcription of 1 μg of RNA was performed with the A3500 Reverse Transcription System Kit (Promega), followed by quantitative real-time PCR on cDNA using the FastStart Essential DNA Green Master kit (Roche ...
-
bioRxiv - Cancer Biology 2021Quote: ... The desired PCR fragments were isolated from a 1% agarose gel and purified using the Wizard Genomic DNA Purification Kit (Promega). In order to identify the specific edition ...
-
bioRxiv - Molecular Biology 2022Quote: ... The SELENOP 3’UTR and the control plasmid were linearized using Bsb 1 and in vitro transcribed using the Ribomax kit (Promega). The RNA was then purified using p30 size exclusion columns (Bio-Rad ...
-
bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted from the beads using the elution buffer (1% SDS and 100 mM NaHCO3) and purified by PCR purification kit (Promega). The final products were analyzed by quantitative real-time PCR with the primers listed below:
-
bioRxiv - Developmental Biology 2021Quote: ... The PCR amplified inserts were run on a 1% agarose gel and purified using the Wizard SV Gel and PCR Clean-Up System kit (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... between 0.5 to 1 µg of total RNA was reverse transcribed into cDNA using an ImProm II Reverse Transcriptase kit (Promega). Equal amounts of cDNA were quantified by RT-qPCR using the IQ SYBR green Supermix (Bio-Rad ...
-
bioRxiv - Molecular Biology 2020Quote: The AP-1 luciferase reporter was expressed using an adenoviral vector and luciferase activity determined using a luciferase assay kit from Promega as previously described (Higazi et al. ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Amplification products were checked on a 1% agarose gel and were purified using the Wizard PCR clean-up kit (Promega). DNA concentration and purity were measured using a ND-1000 UV-Vis Spectrophotometer (Nanodrop Technologies).
-
bioRxiv - Microbiology 2020Quote: ... PCR reaction mixtures were solved in 1% (w/v) agarose gel and purified by means of Wizard SV gel and PCR Clean Up kits (Promega). The amplified gene was treated with Taq polymerase (Fermentas ...
-
bioRxiv - Microbiology 2021Quote: ... as described previously(40) and 1 μg of RNA was reverse transcribed to cDNA using the ImPromIITM Reverse Transcriptase kit (Promega), according to manufacturer’s instructions.
-
bioRxiv - Pathology 2021Quote: RT-qPCR (SARS-CoV-2 and MS2 viral genome detection) were performed with the GoTaq 1-step qRt-PCR kit (Promega) using 3.8µL of extracted RNA and 6.2µL of RT-qPCR mix that contains 250nM of each primer and 75nM of probe ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were harvested day 1 and day 5 post-infection using a CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega) in accordance with the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... 293T cells were co-transfected with envelope-expressing plasmid and an env-deleted HIV-1 backbone plasmid (pSG3ΔEnv) using a FuGENE6 transfection kit (Promega Inc.). Cell supernatants containing pseudotyped viruses were harvested 48 h post transfection and used for infection in TZM-bl cells using DEAE-dextran (25 μg/ml ...
-
bioRxiv - Microbiology 2021Quote: ... HEK293T cells were transiently transfected with plasmid DNA pHIV-1 NL4·3Δenv-Luc and Spike-Δ19-D614G by using Profection mammalian transfection kit (Promega Inc) following the instructions in the kit manual ...
-
bioRxiv - Genetics 2022Quote: ... we added 25 uL Dulbecco’s PBS and 25 uL of reagent 1 from the Dual-Glo luciferase kit (Promega Inc.) and mixed well to lyse the cells ...
-
bioRxiv - Genomics 2022Quote: ... A single colony was used to inoculate LB Broth and genomic DNA extracted from 1 mL using a Promega Wizard kit according to the manufacturer’s instructions (Promega, USA). DNA was quantified using the Qubit 3 and Nanodrop (Thermofisher ...
-
bioRxiv - Microbiology 2023Quote: ... HEK293T cells were transiently transfected with plasmid DNA pHIV-1 NL4·3Δenv-Luc and Spike-Δ19-D614G by using profection mammalian transfection kit (Promega Inc.). After 48 hours ...
-
bioRxiv - Microbiology 2023Quote: RT-qPCRs were performed with MLV SU or 2-LTR primers (Table 1) using a Power SYBR green PCR kit (Promega) and the QuantStudio 5 real-time PCR system (Applied Biosystems) ...
-
bioRxiv - Biochemistry 2023Quote: ... PCR reaction mixtures were solved in 1% (w/v) agarose gel and purified using Wizard SV gel & PCR Clean-Up kits (Promega). Then ...
-
bioRxiv - Developmental Biology 2023Quote: ... Lysates were diluted 1:10 with Milli-Q water and processed for PCR using GoTaq G2 Flexi DNA Polymerase kit (Promega). Reactions were placed in a thermocycler on a touchdown program ...
-
bioRxiv - Microbiology 2023Quote: ... core and modified histones and transcription factors at the HIV-1 LTR was assessed by quantitative PCR using primers spanning the full promoter (Table 1) with GoTaq qPCR Master mix kit (Promega) in a CFX Connect Real-Time PCR thermocycler (BioRad) ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... We isolated and purified genomic DNA from 1 mL of the overnight cultures using the Wizard DNA purification kit (Promega). Short-read sequencing (2 x 150bp paired-end reads ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assayed starting at Day 1 for 6 days according to the CellTiter-Glo 3D kit (Promega G9683). For the antiandrogen response assay ...
-
bioRxiv - Biochemistry 2019Quote: ... Plates were incubated for 30 min at 25 °C before NanoBRET Nano-Glo Substrate (Promega) was added to both control and experimental samples at a final concentration of 10 µM ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... all plates were allowed to reach ambient temperature and the NanoGlo reagent with Furimazine (Promega) was added in 1:1 ratio to the cells ...
-
bioRxiv - Cancer Biology 2020Quote: ... parallel plates were assessed for total dsDNA (above) and total ATP (Cell Titer-Glo, Promega). Cell number by each method was normalized to a standard curve ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... plates were read within 10 min of this addition using a GloMax Discover luminometer (Promega) equipped with 450 nm BP filter (donor ...
-
bioRxiv - Molecular Biology 2019Quote: ... Luciferase activity was measured in 96 well plates using Steady-Glo Reagent (Promega, cat #E2510).
-
bioRxiv - Molecular Biology 2023Quote: ... on the Envision plate reader after cell lysis with 1X passive lysis buffer (E1941, Promega). Results were normalized to the total protein amount per well determined using a BCA protein assay kit (Pierce) ...
-
bioRxiv - Cancer Biology 2023Quote: ... 8,000 cells were plated/well in 96-well plate and Dual Glo Luciferase system (Promega) was used with a luminometer to measure expression levels in light units.
-
bioRxiv - Biochemistry 2022Quote: ... plates were read by measuring luciferase activity using a Nano-Glo Luciferase Assay System (Promega) kit ...
-
bioRxiv - Physiology 2023Quote: ... Fluorescence emission was determined at 520 nm using a fluorescence plate reader (GloMax, Promega, USA). Each sample yielded mean fluorescence intensity units that were converted into epinephrine concentration expressed as μg/g adrenal wet weight using calibration standards and polynomial curve fitting.
-
bioRxiv - Neuroscience 2023Quote: ... Plates were incubated for 48 h and cell viability assay performed (Promega CellTiter-Glo 2.0). Luminescence was recorded using a SynergyH4 microplate reader (Agilent Technologies ...
-
bioRxiv - Immunology 2023Quote: Cells in a 96 well plate were lysed with Glo-Lysis buffer (Promega, 50 µl) and 25 µl cell lysates were mixed with 25 µl Bright-Glo or Nano-Glo (both Promega) ...
-
bioRxiv - Cancer Biology 2023Quote: ... All assays were performed in triplicate in a Glomax multi-detection system plate reader (Promega).
-
bioRxiv - Biochemistry 2024Quote: ... plates were placed for 15 min at RT to equilibrate before NanoBRET NanoGlo Substrate (Promega) was added ...
-
bioRxiv - Cancer Biology 2024Quote: ... plates were equilibrated to room-temperature and 12.5 µL CellTiter-Glo Cell Viability Assay (Promega) reagent added to each well ...
-
bioRxiv - Neuroscience 2021Quote: ... Culture media was then replaced with 50 µL of qNSC/aNSC media containing 1 mM 2DG (2-deoxy-D-Glucose, provided in Glucose Uptake-Glo kit from Promega (J1342)) reagent for 10 minutes in incubator (humidified ...