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Citations for Promega :
551 - 600 of 6157 citations for Cortisol ELISA Kit 1 Whole plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: JAK2-deficient ϒ2A human fibrosarcoma cells were transfected with different human JAK2-hemagglutinin (HA) constructs in pCIneo vector (100 ng per 12-well plate well) with FuGENE HD (Promega). After 48 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... purified by ethanol precipitation and co-transfected into Huh7 cells in 6-well plates (90 ng reporter, 10 ng pcDNA-Blast) using FuGENE 6 transfection reagent (Promega). Cells were selected with blasticidin (0.2 μg/mL ...
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bioRxiv - Biochemistry 2019Quote: ... cells were grown in 96-well polyD-lysine coated black FLIPR plates and transfected using FuGENE HD (Promega, Madison, WI) as the transfection reagent according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Luciferase activity was quantified by transferring 20 μL of lysed cells to a 96-well plate and adding 50 μL of luciferase assay reagent (Promega). Luminescence was measured on a Perkin-Elmer Victor II plate reader ...
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bioRxiv - Microbiology 2021Quote: ... NF54-HGL parasites were treated with 3× IC50 for two weeks in two independent experiments and cultures were monitored by luminescence readout on the BioTek Synergy 2 Plate reader using ONE-Glo reagent (Promega). Whole-genome sequencing was performed to test for single nucleotide polymorphisms (SNPs ...
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bioRxiv - Bioengineering 2021Quote: ... Luminescence was measured with a FluoStar Omega plate reader (BMG Labtechnologies) and treated with the protocol and reagents of the Dual-Luciferase Reporter Assay System (Promega). All reagents were used accordingly to the manufactures protocol.
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bioRxiv - Bioengineering 2020Quote: ... Luminescence from the activity of luciferase was measured 48 h post-infection using a TECAN infinite M200 pro plate reader after applying Bright-Glo reagent (Promega) on cells.
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bioRxiv - Neuroscience 2019Quote: ... in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μL of cell lysate was transferred to 96-well plates and placed in a 96-well microplate luminometer (GloMax-Multi, Promega). 100 μL Luciferase Assay Reagent II and 100 μL Stop & Glo Reagent (both provided in the Dual-Luciferase Reporter kit ...
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bioRxiv - Cancer Biology 2020Quote: ... and 50 μl of diluted media added to a white 96 well plate (CLS3610) before the addition of 50 μl of Lactate/Glucose detection reagent (Promega). Plates were incubated for 1 h at RT and luminescence recorded (Glowmax Luminometer ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell lysates were transferred to a 96-well black plate and then an equal volume of Luciferase assay reagent (Promega) was added ...
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bioRxiv - Biochemistry 2021Quote: ... and firefly luciferase expression was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). Percentage of infectivity was calculated as the ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Microbiology 2020Quote: ... the plate was incubated at room temperature for 10 minutes to stabilize luminescent signal before luminescence was recorded on a GloMax plate reader (Promega).
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated for 30 minutes in the dark before being read at 405 nm on a GloMax Explorer (Promega).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were seeded in a 6-well plate and transfected with 750 ng of each of the AT1R-TANGO plasmids using FUGENE 6 Transfection (PROMEGA). The following day ...
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bioRxiv - Microbiology 2021Quote: ... The cells were transferred to white- walled 96-well plates and processed using the Bright-Glo Luciferase Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... lysates were transferred into white 96-well plates and FLuc activity was measured using a commercial substrate (Beetle-Juice, PJK; Luciferase Assay System, Promega) and a plate luminometer (Hidex Sense Plate Reader ...
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bioRxiv - Molecular Biology 2021Quote: ... Luciferase assays were performed on 20 μl of lysate or 1x Cell Culture Lysis Reagent in 96 well plates on the GloMax-Multi Detection System (TM297, Promega). 100 μl Luciferase Assay Reagent was added to wells ...
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bioRxiv - Microbiology 2020Quote: ... 2×105 HeLa-HA cells were seeded per well of a six-well plate and transfected using Fugene-HD transfection reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Plates were frozen for at least 30 minutes at −80°C before thawing and reading on a GloMax Multi plate reader (Promega). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Two Days after infection media was removed from the cells and at least 50μl of the CellTiter-Glo® Reagent was then quickly added to all wells in the luminometer plate and the luciferase reading was quantified with a Veritas luminometer (Promega). The cytopathic effect was quantified by the CellTiter-Glo Luminescent Cell Viability Assay system (Promega ...
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bioRxiv - Cancer Biology 2020Quote: HF3016 cells were plated in individual wells of a 96-well plate and viable cells were quantified using CellTiter-Glo 3D Cell Viability Assay (Promega) in triplicate wells as per manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... mass ratio) into LentiX cells grown to approximately 80% confluency in 6-well plates using FuGENE HD Transfection Reagent (Promega) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... 140 mM NaCl and 1% Triton X-100) and lysates used to assay luciferase activity in 96-well plates using the Dual-Luciferase Reporter Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 25,000 leukemia cells in 250µL of drug-containing medium were seeded in a 48-well plate and viability was assessed every 4 days by using the CellTiter-Glo Luminescent Cell Viability assay (Promega). For RNA-Seq and ChIP-Seq experiments ...
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bioRxiv - Microbiology 2022Quote: ... The plate was incubated in the dark for 2 min and then read on a Glomax Multi+ Detection System (Promega) as above ...
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bioRxiv - Cell Biology 2022Quote: ... The cell plates were incubated at 37°C for 48-60 hours before 50 μL of CellTiter-Glo reagent (Promega) was added ...
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bioRxiv - Microbiology 2022Quote: ... The expression of firefly luciferase was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of neutralization was calculated as the ratio of the reduction in luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Microbiology 2022Quote: ... was mixed 1:1 with cells seeded in 96-well plates for three consecutive days and the luminescence signal was quantified using the GloMax-Multi microplate reader (Promega). A list of all used sgRNA sequences and genotyping primers can be found in Table S4.
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bioRxiv - Biophysics 2022Quote: ... mass ratio) into LentiX cells grown to approximately 80% confluency in 6-well plates using FuGENE HD Transfection Reagent (Promega) per manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... the plates were equilibrated to room temperature and the culture media was replaced by the Nano-Glo HiBiT extracellular reagent (Promega), which was prepared according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were not selected with puromycin but parallel plates were treated with puromycin or left untreated and then cell viability was determined using CellTiterGlo reagent (Promega) for calculation of infection efficiency ...
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bioRxiv - Cancer Biology 2022Quote: ... 25 μL of each were then transferred to a fresh white 96-well plate containing 25 μL of GSH-Glo mixtures (Promega). Luminescence was determined using a Tecan Spark 10 microplate reader
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bioRxiv - Microbiology 2020Quote: ... 20μl of each sample was read on Fluo Star Optima plate reader with injection of 50 μl of Luciferase Assay Reagent (Promega).
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... was performed following the manufacturer’s instructions and absorbance was measured at 490nm using a GloMax-Multi Plate Reader (Promega, E6521). Three independent experiments were performed ...
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bioRxiv - Cell Biology 2020Quote: ... 50 or 100 μl of supernatants was transferred to 96-well plates and ATP was measured using the CellTiter-Glo Luminescent Cell Viability Assay (Promega) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... and the luminescent signal was measured using a CentroXS3 plate reader (Berthhold Technologies) or GloMax explorer multimode microplate reader 3500 (Promega).
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bioRxiv - Biochemistry 2021Quote: ... HEK293T cells and C3H10T1/2 cells were transfected in 6-well plates with various constructs of pmirGlo vector (Promega®). Transfection was performed using Turbofect transfection reagent (Thermo Fischer scientific® ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected in 96-well plates with 98 ng rTetR plasmid and 2 ng pRL Renilla Luciferase control reporter plasmid (Promega) to control for transfection efficiency ...
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bioRxiv - Immunology 2022Quote: ... Cells were transferred to a white-walled 96-well assay plate and mixed with an equal volume of Cell Titer Glo reagent (Promega). Measurements for fluorescence at 544-15 nm (excitation) ...
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bioRxiv - Systems Biology 2022Quote: ... and 72h in 3-5 wells for each condition (mean values of 3-5 technical replicates are provided for each donor) using a multiwell plate reader Glomax (Promega). Each 2h incubation was performed at a multiplicity of infection (MOI ...
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bioRxiv - Immunology 2022Quote: A total of 200,000 RAW cells were seeded on a 24-well plate and were co-transfected with NFATc1(pGL3-NFAT) and Renilla luciferase plasmid (pRL-TK, Promega) in a ratio of (2:1) ...
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bioRxiv - Microbiology 2022Quote: ... was added to the plate and incubated at room temperature for 5 min before measuring luminescence on a GloMax 96 luminometer (Promega).
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bioRxiv - Microbiology 2022Quote: ... and transferring the contents to a 96-well plate to which the appropriate substrate was added and assayed using a GloMax multi system (Promega).
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bioRxiv - Molecular Biology 2019Quote: ... 100,000 cells per well we seeded on glass coverslips in 6-well plates and transiently transfected after 24 hours using Fugene6 (Promega) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... cell supernatants were used to monitor GLuc levels in Nunc 96-well optical-bottom plates (ThermoScientific) using Dual-luciferase reporter assay system (Promega) as per manufacturer’s protocol while the harvested cell lysates were assayed for GFP levels in Varioskan microplate reader (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2020Quote: ... RL and FL activities were measured on 96-well plates using 2 µl of cell extract containing 8 µl of 1x PLB (Promega) and the dual-luciferase assay kit (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... Luciferase activity present in 10 µl of lysate clarified by centrifugation was then quantitated in white 96-well luminometer plate (Microlumat Plus 1b 96V instrument, Berthold Technologies) using 50 µl of luciferase assay reagent (Promega).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 40 μl of the cell suspension were transferred to each well of 96-well plates containing 10 μl of 5X GloSensor cAMP Reagent (Promega) prepared according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2019Quote: 3T3-L1 cells were seeded at a density of 1,5 x 104 cells in 24-well culture plate and 24 hours later cells were transfected using ViaFect transfection reagent (Promega). The total amount of DNA used for transfection per well was 0,8 μg ...