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Citations for Promega :
1 - 50 of 2863 citations for Ciprofloxacin Hcl 100 Ug Ml In Methanol 2 3 Carboxyl 13C3 99%; Quinoline 15N 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The bound proteins were digested by resuspending the beads in 200 μL freshly prepared digestion buffer (2M Urea, 100 mM Tris-HCl pH 8, 100 mM DTT) with 0.5 ug trypsin (Promega, V5111) and overnight incubation at room temperature while shaking ...
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bioRxiv - Biochemistry 2022Quote: ... 20 ug/ mL chymotrypsin (Promega), 1 mM CaCl2 ...
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bioRxiv - Molecular Biology 2022Quote: ... with 100 ug Fugene (Promega, Cat#E2311) in 1.5 mL complete Sf9 medium and added to 100 M Sf9 cells resuspended in 30 mL complete Sf9 medium ...
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bioRxiv - Microbiology 2020Quote: ... Urea was diluted to 2 M with 100 mM Tris-HCl pH 7.0 and trypsin (Promega) was added at a mass ratio of 1:100 (trypsin:protein) ...
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bioRxiv - Biochemistry 2022Quote: ... 10 ug/mL sequencing grade trypsin (Promega), 20 ug/ mL chymotrypsin (Promega) ...
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bioRxiv - Systems Biology 2020Quote: 100 mM TEAB and 5 ug trypsin (Promega V5113)
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bioRxiv - Molecular Biology 2023Quote: ... 1M Tris-HCl was added to adjust the pH and 5 μL trypsin (2.5 ug, Promega Sequence Grade) was added and the samples digested overnight at 37℃ ...
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bioRxiv - Cancer Biology 2022Quote: SJ-GBM2 and SF8628 cells were used to determine if reducing WDR82 through inducible knockdown affected cell viability 1×104 cells/100μl were plated in 96-well plates with complete cell culture medium with or without Dox (2μg/ml), and subjected to 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, Promega) assay.
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bioRxiv - Cell Biology 2023Quote: ... dilute with 4 volumes of 20 mM Tris-HCl pH8 / 2 mM CaCl2 before overnight digestion at room temperature with 100 ng sequencing grade trypsin (Promega). Samples were centrifuged ...
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bioRxiv - Immunology 2022Quote: ... The gel pieces were resuspended in 0.1 ml of 100 mM Tris/HCl pH 8.0 containing 10 mM calcium chloride and chymotrypsin (Promega, #V1061) for 16 h at 25 °C ...
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bioRxiv - Immunology 2021Quote: ... after added 100 mL of Caspase-Glo 3/7 reagent (Promega, USA), the plate was gently shaken at room temperature for 2 h and the luminescence was measured by a plate-reading luminometer.
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bioRxiv - Cell Biology 2019Quote: ... proteins were trypsinised on the beads in 60 μl of Buffer I (2 M urea, 50 mM Tris-HCl pH 7.5, 5 μg/ml Trypsin [modified sequencing-grade trypsin; Promega]) for 30 min at 37°C in a thermomixer ...
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bioRxiv - Neuroscience 2019Quote: ... 100 units/mL penicliin and 100 ug/mL streptomycin in 96 well plates (Falcon 353072) and transfected with individual K2Ps using Fugene HD (Promega, Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Treatment with Proteinase K was carried out in 500 μl of PK Buffer (100 mM Tris-HCl pH8; 50 mM NaCl; 10 mM EDTA; Proteinase K 4 mg/ml; RNasin PROMEGA) 2 h at 55 °C and 15 min at 95 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Three washes were performed with ChIP Dilution Buffer as in DRIP assays before incubating the IP fraction with Proteinase K for 2 h at 55°C plus 15 min at 95°C in PK Buffer (100 mM Tris-HCl pH8; 50 mM NaCl; 10 mM EDTA; 4 mg/ml Proteinase K; RNasin PROMEGA). We added 2 U DNase I (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... 40 IU/ml penicillin and 40 ug/ml streptomycin containing RealTime Glo reagent (Promega) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... 1 mM CaCl2 with 2 ug trypsin (Promega, Madison, WI, product V5111). Digest was stopped with formic acid ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Triton X-100, 2 mM DTT, 100 μg ml-1 cycloheximide [Sigma], 20 U ml-1 RNase inhibitor [Promega] ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted with 4 volumes of 20 mM Tris-HCl pH 8 2 mM CaCl2 prior to overnight digestion with 100 ng sequencing grade trypsin (Promega, Cat# V5111) at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... cells were treated with the LRRK2 inhibitor MLi-2 (100 nM) for 2 hours and then labelled with the TMR ligand (3 μM)(Promega). After 15 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... Transfected cells were selected with 750 ug/ml G418 (Promega Corp., Madrid, Spain) and 0.4ug/ml Puromycin (Invitrogen).
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bioRxiv - Neuroscience 2022Quote: ... Five µL of Sequencing Grade Trypsin (100 ng/µl in 10 mM HCl, Promega) were added to the beads and the digestion was carried out in a microwave instrument (Discover System ...
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bioRxiv - Cell Biology 2023Quote: cDNA was synthesized from 2 ug of total RNA using GoScript Reverse Transcriptase (Promega, #A5003) with random hexamers according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... and suspended LysC solution (Promega, 12.5ng/mL in 25mM Tris-HCl pH 8.5). Digestion was performed overnight at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected in 96-well plates with 98 ng rTetR plasmid and 2 ng pRL Renilla Luciferase control reporter plasmid (Promega) to control for transfection efficiency ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900 μL of 0.35 M KCl (10 mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900μL of 0.35M KCl (10mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900μL of 0.35M KCl (10mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
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bioRxiv - Biochemistry 2022Quote: ... 3 units/mL RNasin (Promega). Depending on the experiments (see results) ...
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bioRxiv - Genomics 2020Quote: ... 3 ug of pCAG-NLS-Bxb1 was diluted in 250 uL of OptiMEM and 6 uL of Fugene (Promega). On day 2 ...
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bioRxiv - Microbiology 2023Quote: ... for 2 hours at 72 hours and lysed at 98 hours using 25 μl Dual-Glo® Luciferase Assay System (Promega). Luminescence was measured ...
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bioRxiv - Molecular Biology 2020Quote: ... 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) (Promega, Cat. No. G109C) cell viability assay was performed ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mM KCl, 5 mM MgCl2, 2 mM DTT, 100 μg ml-1 cycloheximide, and 20 U ml-1 RNase inhibitor [Promega].) Gradients were centrifuged 36,000 rpm for 2.5 h in a SW41 Ti rotor (Beckman Coulter ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transfected with 2 ug of pQCXIN ERAAP-dsRed using Fugene HD transfection reagent (Promega, E2311) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... We added 300μl of Proteinase K Buffer 2X (200 mM Tris-HCl pH7.5; 100 mM NaCl; 20 mM EDTA; RNasin PROMEGA 20 U/μl; 0.04 mg/ml Proteinase K) and incubated for 1 h at 55 °C ...
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bioRxiv - Microbiology 2023Quote: ... 20 μL of MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) inner salt (Promega) was added to each well and the plate was further incubated for 1 h at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 μL of [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] (MTS) (Promega, #G3582) were added and incubated at 37°C for 3 hours ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were precipitated with 0.1 M ammonium acetate in 100% methanol and the protein pellets were further digested with sequencing-grade trypsin (Promega). For the analysis involving the rpsAΔ6 mutant missing the last C-terminal OB fold domain of protein S1 ...
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bioRxiv - Plant Biology 2020Quote: ... proteins were precipitated overnight with 5 volumes of cold 0.1 M ammonium acetate in 100% methanol and digested with sequencing-grade trypsin (Promega) and each sample was analyzed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo Fisher) ...
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bioRxiv - Cell Biology 2019Quote: ... nuclei from HepG2 cells were isolated by incubating cell pellets in 500 μl of a hypotonic buffer for 15 min on ice (20 mM Tris-HCL, pH 7.4, 10 mM NaCl, 3 mM MgCL2, with protease and phosphatase inhibitor cocktail from Promega). Triton X-100 detergent was added to a 0.5% v:v final concentration to lyse the cells followed by centrifugation at 1200 × g for 10 min to pellet nuclei ...
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bioRxiv - Plant Biology 2021Quote: ... The RIP fraction was washed with Washing Buffer (0.3 M NaCl; 20 mM Tris-HCl pH7.5; 5 mM MgCl2; 5 mM DTT; protease inhibitor tablet; RNasin PROMEGA) three times ...
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bioRxiv - Plant Biology 2021Quote: ... 2 ug of RNA were reverse transcribed using a reverse transcription kit (GoScript™ Reverse Transcription System, Promega). The abundance of specific transcript was measured by probing 1 μL cDNA by quantitative real-time PCR in a total volume of 10 µl containg 5 μL SYBR-green master mix (Roche) ...
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bioRxiv - Immunology 2021Quote: Transfection was done in BEGM media in 96-well dishes (65-85% confluent) with 0.098 ug CLEC7A.luc and 0.002 ug pGL4.PGK control vector per well (Promega) using Trans-IT X2 (Mirus) ...
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bioRxiv - Microbiology 2020Quote: ... Samples were diluted with three volumes digestion buffer (100 mM Tris/HCl pH 8.5, 1 mM CaCl2) and 0.25 μg Trypsin (Sequencing Grade, Promega) were added and incubated o/n at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... The samples were then diluted with Tris-HCl (100 mM, pH 8.0) and digested with 0.5 µg sequencing-grade trypsin (Promega). The generated peptides were desalted and analyzed in duplicates by LC-MS/MS using a timsTOF fleX mass spectrometer equipped with a nanoElute LC system (Bruker) ...
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bioRxiv - Genetics 2023Quote: ... proteins were extracted by chloroform-methanol precipitation and digested with 2 μg trypsin/Lys-C mixture (Promega, Madison, WI). Samples were labeled with 10-plex tandem mass tag reagents (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 100 µL/well of Caspase 3/7 reagent (Promega) was added per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... 100 U/ml RNasin (Promega) and protease inhibitor cocktail (Roche) ...
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bioRxiv - Physiology 2019Quote: ... 100 U/mL RNasin (Promega), 100 μg/mL cycloheximide ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 U/mL RNasin (Promega), complete protease inhibitor (Roche)] ...