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Citations for Promega :
1 - 50 of 1203 citations for Chromosome 10 Open Reading Frame 82 C10ORF82 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: Open reading frames of OR genes were subcloned into pCI (Promega) with a Rho tag at the N terminal ...
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bioRxiv - Biophysics 2022Quote: ... pHalo-PR open reading frame (ORF) clone was purchased from Promega (Promega #FHC24423). pHalo-ER expresses human ERα fused to HaloTag in the C-terminus regulated by a CMVd1 promoter and has been described previously (21 ...
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bioRxiv - Developmental Biology 2020Quote: Whole open reading frame of eco1 and eco2 where cloned into pGEM-T (Promega). DIG-labeled probes where generated using T7/SP6 transcriptions start sites and the digoxigenin RNA Labeling Mix kit (Roche ...
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bioRxiv - Neuroscience 2022Quote: Open reading frames of human OR genes were subcloned into pCI (Promega, WI, USA) with a Rho-tag (the sequence encoding the first 20 amino acids of rhodopsin ...
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bioRxiv - Bioengineering 2021Quote: The open reading frame for human SOX17 was PCR amplified using GoTaq Master Mix (Promega) from the PB-TRE3G-SOX17 plasmid (Table S5) ...
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bioRxiv - Microbiology 2022Quote: ... the mKate2 open reading frame was replaced with that of the nanoluciferase gene from pNL1.2[NlucP] (Promega) and the virus was generated as indicated above ...
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bioRxiv - Immunology 2021Quote: ... The RARα open reading frame (NP_000955) was sub- cloned from the original plasmid (pFN21AE1591, Promega KK, Tokyo, Japan) into the pFC14K plasmid (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: ... the entire open reading frame was inserted into the plasmid backbone from pFN24A HaloTag CMVd3 Flexi Vector (Promega) so that the ORF was under a CMV-d3 minimal expression promoter ...
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bioRxiv - Molecular Biology 2021Quote: ... was previously constructed by inserting a 351 bp-TiLV segment 9 open reading frame (ORF) into pGEM T-easy vector (Promega) as reported earlier (Thawornwattana et al ...
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bioRxiv - Molecular Biology 2021Quote: ... 3’UTR PCR products were directionally cloned downstream of the Renilla luciferase open reading frame (ORF) of the psiCHECK2 vector (Promega) that also contains a constitutively expressed firefly luciferase gene ...
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bioRxiv - Biophysics 2024Quote: ... was incorporated at the 5’ end of the FL open reading frame during PCR amplification from a pRL-CMV vector (Promega). A Kozak consensus sequence and a 50-nucleotide upstream region was incorporated before the translation start site to ensure enough space for the assembly of translation initiation complex77 ...
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bioRxiv - Microbiology 2022Quote: ... from Huh7 genomic DNA and inserted the promoter element upstream of the firefly luciferase open reading frame in the pGL2-Basic vector (Promega Corp.). In all cases ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The resulting RT-PCR amplicons of the stomach sample (full open reading frame sequence for GRP or partial sequence for GRPR) were subcloned into the pGEM-T easy vector (Promega, Madison, WI) followed by transfection into Escherichia coli DH5α competent cells (Takara Bio ...
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bioRxiv - Developmental Biology 2023Quote: ... Germany)-labeled antisense riboprobe was synthesized from the plasmid containing the full or partial length open reading frame of the cDNAs using SP6 or T7 polymerase (Promega, Madison, WI, USA) after restriction enzyme digestion (New England Bio Labs ...
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bioRxiv - Microbiology 2020Quote: ... We replaced the mCherry reading frame with nano-luciferase (nLuc-VLP) (Promega) or codon optimized GFP (GFP-VLP) ...
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bioRxiv - Neuroscience 2022Quote: ... the used PCR primers were flanked with SfiI and NheI sites to allow the insertion in front of hRluc reading frame in psiCheck-2 vector (Promega).
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bioRxiv - Microbiology 2023Quote: ... Luciferase reading was taken with Glomax Luminometer (Promega).
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bioRxiv - Microbiology 2022Quote: ... and the Gaussia princeps luciferase enzymatic activity was measured on a Centro XS LB960 microplate luminometer (Berthold Technologies, reading time 10 s after injection of 50 µl Renilla luciferase reagent (Promega). For the minigenome assays ...
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bioRxiv - Microbiology 2023Quote: ... princeps luciferase enzymatic activity due to luciferase reconstitution was measured on a Centro XS LB960 microplate luminometer (Berthold Technologies) using a reading time of 10 s after injection of 50 µl Renilla luciferase reagent (Promega). Mean relative light units (RLUs ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.5 U of the proof-reading Pfu polymerase (Promega) with 30 cycles of 94°C for 30s ...
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bioRxiv - Microbiology 2019Quote: ... Readings were performed with a Glomax 20/20 system (Promega). For each experiment ...
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bioRxiv - Microbiology 2021Quote: ... reading luminescence on a GloMax ®-Multi Detection System (Promega). For each condition ...
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bioRxiv - Molecular Biology 2020Quote: ... Chromosome 4: 101,716,750-101,718,250 forward strand) was cloned into pGL3-basic vector (E1751, Promega). Reporter vector ...
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bioRxiv - Cancer Biology 2021Quote: ... Luminescence reading was performed using GloMax® Discover Microplate Reader (Promega).
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bioRxiv - Cancer Biology 2019Quote: ... Readings were done in triplicate using GloMax Navigator Microplate Luminometer (Promega) and values were normalized for transfection efficiency using co-transfected renilla luciferase plasmid.
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bioRxiv - Neuroscience 2024Quote: ... The luminescence readings were acquired using a GloMax Microplate Reader (Promega). The percentage of viability was determined by normalizing the raw luminescence values to those of the vehicle condition (DMSO-treated wells ...
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bioRxiv - Biochemistry 2020Quote: ... Luciferase readings were performed using the Dual Luciferase Reporter Assay System (Promega) on a Biotek Synergy Neo2 plate reader ...
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bioRxiv - Developmental Biology 2022Quote: ... Luminescence readings were carried out on a GloMax Multi Detection System (Promega). Luciferase readings were normalized to the corresponding Renilla values.
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bioRxiv - Microbiology 2023Quote: ... Luminescence readings were performed using a Glomax 20/20 Luminometer system (Promega). Each experiment contained a minimum of three technical replicates and experiments were repeated at least three times ...
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bioRxiv - Cell Biology 2023Quote: ... RLU reading was performed with a microplate reader (Promega Glomax Multi Detection) using the luminescence protocol.
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bioRxiv - Microbiology 2023Quote: ... Luciferase readings were conducted with a luminometer (GLOMAX™, Promega, Southampton, UK) after a 5-min incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... Firefly and Renilla luciferase readings were recorded 48 hours posttransfection using Luminometer (Promega). The firefly luciferase activity is then normalized to the Renilla luciferase activity ...
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bioRxiv - Developmental Biology 2023Quote: ... and luciferase readings were acquired using the automated dual injector GloMax Luminometer (Promega). Relative WβS levels were estimated by normalizing
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bioRxiv - Microbiology 2023Quote: ... Standard curves were generated using purified genomic stocks (HSV-1 bacterial artificial chromosome and human genome Promega #G1471). Absolute copy number of genomic stocks was determined using droplet digital PCR (Biorad QX600) ...
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bioRxiv - Biochemistry 2023Quote: NSD2 full-length template was cloned in frame into a pNLF1 vector (Promega) using the In-Fusion HD Cloning kit (Takara ...
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bioRxiv - Cell Biology 2019Quote: ... were transfected with eSpCas9(1.1)-sgCHRXpYp-Subtel2 (pMTH393) that targets chromosome Xp subtelomere locus using FuGENE HD reagent (Promega). Two days post transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Supernatant was collected and used for taking the luciferase reading using Dual Luciferase kit (Promega) as per manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... Firefly luciferase and Renilla luciferase readings were obtained using the Dual-Luciferase Reporter Assay System (Promega) and LUMAT LB 9501 luminometer (Berthold Technologies GmbH & Co ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Readings were then taken every 30 minutes for 48 hours using a GloMax Luminometer (Promega Corporation).
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bioRxiv - Molecular Biology 2019Quote: ... and set at room temperature for 30 min prior to reading with a GloMax luminometer (Promega). Integration time was set at 1 second 54,55.
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bioRxiv - Neuroscience 2024Quote: ... The plate was incubated for 40 min at RT before reading luminescence using GloMax Explorer (Promega) plate reader ...
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bioRxiv - Genomics 2020Quote: ... Luminescence readings for both firefly and renilla were measured via plate reader (Varioskan LUX; Promega DualGlo #E2920). Firefly and renilla values were first blank-subtracted (untransfected cell lysate ...
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bioRxiv - Microbiology 2022Quote: ... RSV replication was evaluated by reading nano-luciferase activity with the Nano-Glo Luciferase Assay system (Promega) on a Tecan Spark plate reader ...
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bioRxiv - Microbiology 2021Quote: ... and the Nano-Luc reading was measured using the Nano-Glo® Luciferase Assay System (Promega, N1150), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Luciferase readings were measured 48 hours’ post-transfection using the Dual-Glo® Luciferase assay system (Promega), as per manufacturer’s instructions using the Centro XS3 LB960 system (Berthold Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... 48h after transfection and luciferase reading was taken using the Dual-Glo luciferase assay kit (Promega, Cat # E1910) as per the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... Luciferase readings were recorded 36 hours post-transfection using the Dual luciferase reporter assay kit (Promega; cat.no. E1910) on Perkin Elmer’s Multimode plate reader (Perkin Elmer ...
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bioRxiv - Molecular Biology 2021Quote: ... and the 82-nt exon 11 of the MAPT gene was constructed in the pCI-neo vector (Promega).
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bioRxiv - Molecular Biology 2023Quote: Full-length VHL was obtained as plasmids cloned in frame with a terminal NanoLuc-fusion (Promega, ND2700). The plasmid was transfected into HEK293T cells using FuGENE HD (Promega ...
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bioRxiv - Microbiology 2020Quote: ... viral particles were purified by cesium chloride gradient centrifugation 82 and DNA extracted with Wizard Prep Columns (Promega, Corp.). Viral metagenome short-read libraries were prepared using the NexteraXT kit (Illumina ...