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Citations for Promega :
251 - 300 of 1008 citations for C terminal Proghrelin Isoform Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... or a combination of trypsin and Glu-C (Promega); aliquots of etanercept proteins were digested respectively using trypsin (Promega) ...
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bioRxiv - Biochemistry 2022Quote: ... or a combination of Glu-C and AspN (Promega). For the analysis of deglycosylated glycopeptides ...
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bioRxiv - Neuroscience 2023Quote: ... After overnight digestion using Trypsin/Lys-C (Promega, #V5073), supernatant was collected from the magnetic beads ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were digested first by Lys-C (Promega, USA) at RT for 2 hours ...
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bioRxiv - Microbiology 2023Quote: ... which were digested with 1 µg Lys-C (Promega) overnight at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... After overnight digestion with Trypsin/Lys-C mix (Promega), peptides were transferred ...
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bioRxiv - Biochemistry 2023Quote: ... then with trypsin (Promega; 1:50, 37°C overnight). Samples were purified by solid-phase extraction (SepPak tC18 cartidges ...
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bioRxiv - Cancer Biology 2024Quote: ... and Trypsin/Lys-C Mix (Promega, Madison, WI, USA) at a 1:50 ratio in a ThermoMixer C (Eppendorf ...
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bioRxiv - Molecular Biology 2024Quote: ... Trypsin/Lys C was purchased from Promega (Madison, USA). ITO slides were obtained from Delta technologies (Loveland ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested using trypsin/ Lys-C mixture (Promega) and desalted on a 10 x 4.0 mm C18 cartridge (Restek ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5 µg of trypsin/Lys-C mixture (V5073, Promega) added to each on-bead sample and set to rotate head-over-head overnight at RT ...
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bioRxiv - Microbiology 2024Quote: ... and pBiT2.1-C[TK/SmBiT] were obtained from Promega. ALTO ...
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bioRxiv - Systems Biology 2021Quote: ... Collisional energy was ramped stepwise as a function of ion mobility.24 Peptide loading was normalized to the total ion chromatograms from 200 ng injections of K562 whole cell lysate standard (Promega).
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bioRxiv - Biophysics 2020Quote: ... Proteins were digested to peptides by addition of 25 μl of 50 mM ammonium bicarbonate containing 5 μg of trypsin (Promega) and incubating (37°C ...
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bioRxiv - Immunology 2020Quote: The strategy for genome-engineering to tag HLA-B with the HiBit peptide was developed based on a previous publication from Promega Corporation ...
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bioRxiv - Biochemistry 2019Quote: A commercial K562 cell digest supplemented with the 6 x 5 LC-MS/MS Peptide Reference Mix (QC4Life Reference Standard, Promega) was used as quality control (QC ...
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bioRxiv - Bioengineering 2019Quote: ... after removal of its original signal peptide and was further fused to Halo-tag derived from HaloTag Control Vector (Promega) at the C-terminus ...
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bioRxiv - Synthetic Biology 2019Quote: ... The supernatant was taken to measure the amount of secreted HiBiT peptide using the Nano-Glo HiBiT Extracellular Detection System (Promega).
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bioRxiv - Synthetic Biology 2019Quote: ... and the supernatant was taken to measure the amount of secreted HiBiT peptide using the Nano-Glo HiBiT Extracellular Detection System (Promega). The resulting luminescence signals were normalized based on the cell viability that was measured using the PrestoBlue Cell Viability Reagent (Thermo).
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bioRxiv - Cell Biology 2022Quote: Methylation of peptides (PSL, Heidelberg, Germany, Table S5) was performed using the bioluminescence-based MTase-GloTM Assay (Promega, Cat#V7601) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... peptides were diluted 4-fold with ABC buffer (Ammonium bicarbonate 50 mM pH8) and digested with Trypsin MS grade (Promega) overnight ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was removed and tryptic peptides were eluted from the beads by overnight digestion in 50 µl trypsin solution (V5111, Promega, 0.01 µg/µl in 100 mM ammonium bicarbonate ...
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bioRxiv - Molecular Biology 2021Quote: ... protein digestion was performed by 1 µg of Lys-C (Wako) at 37 °C for 3 h following 1 µg of trypsin (Promega, Madison, WI, USA) at 37 °C for 16 h ...
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bioRxiv - Microbiology 2023Quote: ... After five-fold dilution with 50-mM ammonium bicarbonate, the sample was digested using Lys-C (Wako, Osaka, Japan) at 37°C for 3 h following trypsin (Promega, Madison, WI, USA) at 37°C for 16 h ...
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bioRxiv - Microbiology 2020Quote: ... The amplified signal peptide fragments were introduced into the plasmid pSUC2 (Prof. Sophien Kamoun, The Sainsbury Laboratory) using T4 DNA ligase (Promega, USA). The yeast invertase assay was used to validate the signal peptides (Jacobs et al ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Stimulation of P-gP ATPase activity was utilized to indicate the interaction between peptides and P-gP using the P-gP-Glo Assay System (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... 1.15 μg of purified AQP2 or AQP2 S256A COOH-tail peptide were mixed with either RSK (16 nmol) or PKA catalytic subunit (5.6 nmol) (Promega, Madison, WI) in a total volume of 12 μl reaction buffer containing 40 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... 500-ng of Trypsin/Lys-C Mix (Promega Cat#V5072) was added and allowed to digest overnight for ∼18-hr at 37°C ...
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bioRxiv - Genomics 2021Quote: ... Digestion was performed overnight at 37°C with trypsin (Promega V5113 ...
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bioRxiv - Plant Biology 2020Quote: ... in 50 mM NH4HCO3 or Glu-C (sequencing grade, Promega) in 50 mM Tris/PO4 ...
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bioRxiv - Cell Biology 2020Quote: ... Digestion was performed using mass spectrometry grade Lys-C (Promega) at a protein:Lys-C ratio of 100:1 (w/w ...
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bioRxiv - Biochemistry 2022Quote: ... 50 mM Tris pH 8 overnight at 37 °C (Promega) at an estimated protein:enzyme ratio 1:100 ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... followed by 3 h at 37°C using trypsin (Promega). All proteolytic digests were acidified to pH 2 by addition of 10% formic acid and directly analyzed by LC-ESI-MS/MS ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested overnight at 37 °C with trypsin (Promega) with an enzyme ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM CaCl2 and 500 ng Trypsin Lys-C (Promega). On bead digest was carried out overnight at 37°C while shaking at 200 rpm ...
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bioRxiv - Cancer Biology 2022Quote: ... and digested 16 hours at 37°C with trypsin (Promega) and stopped by addition formic acid (FA ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were digested by Trypsin/Lys-C Mix (Promega). After the digestion ...
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bioRxiv - Molecular Biology 2022Quote: ... firstly with Endoproteinase Lys-C (ratio 1:33 enzyme:lysate) (Promega) for 1 hour at room temperature then with trypsin (ratio 1:33 enzyme:lysate ...
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bioRxiv - Neuroscience 2023Quote: ... In-gel digestion with Trypsin/Lys-C Mix solution (Promega) was performed as previously described (Gonzalez-Lozano & Koopmans ...
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bioRxiv - Neuroscience 2022Quote: ... 100 µL of 0.1 µg/µL Trypsin/rLys-C (Promega) in 100 mM ammonium bicarbonate (pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing-grade porcine trypsin/Lys-C mix (Promega, Madison, WI) was added ...
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bioRxiv - Molecular Biology 2023Quote: ... and then sequencing-grade endopeptidase Trypsin/Lys-C Mix (Promega) was added at an enzyme–substrate ratio of 1:50 wt/wt ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight at 25L°C with trypsin (Promega) with an enzyme/substrate ratio of 1:250 ...
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bioRxiv - Physiology 2023Quote: ... and digested (0.4 ug of Lys-C/Trypsin mix, Promega). After 4 hours of digestion in the urea buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested overnight at 37°C with trypsin (Promega) and Lys-C (Wako,125-05061 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were then incubated with Lys-C/trypsin mixture (Promega) (200 ng for pull-down samples and 1 ug for total lysate samples ...
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bioRxiv - Biochemistry 2024Quote: ... and Glu-C (V1651) were obtained from Promega (Finnboda, Sweden).
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bioRxiv - Genetics 2019Quote: ... and digestion were performed by dithiothreitol (DTT, 10mM, 30min at room temperature), iodoacetamide (IAA, 10mM, dark for 30min at 37°C) and trypsin (MS Grade, Promega, 12.5ng/ul, 37°C overnight) sequentially before analyzing by Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer ...
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bioRxiv - Molecular Biology 2023Quote: ... 30 min at 60°C) an alkylation (IAA 0.5 M, 30 min RT) proteins were digested using trypsin (Gold, Promega, 1ug / sample, overnight at 37°C). For LC MSMS analysis ...
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bioRxiv - Immunology 2023Quote: ... Urea concentration was further adjusted to 2M using 50mM Tris pH 8 and Lysyl peptides were additionally digested with sequencing grade trypsin (Promega, Madison, WI) in an enzyme/substrate ratio of 1:20 at 37°C overnight ...