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Citations for Promega :
501 - 550 of 1302 citations for Anti PI 4 kinase II beta Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... one microgram of RNA was reverse transcribed using the ImProm-II Reverse Transcription System (Promega). Quantitative RT-PCR was performed in triplicate with custom-designed oligos using the CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... one microgram of RNA was reverse transcribed using the ImProm-II Reverse Transcription System (Promega), thereafter qRT-PCR was performed in triplicate with custom designed oligos (Table S3 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and reverse transcription was carried out using the Improm-II reverse transcriptase (Promega, Medison, USA) under the conditions described in the kit manual ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were incubated in PBDS for 3 days at 4°C followed by 4 washes over the next 24 hours with 0.2% Tween-20 (Promega UK Ltd; H5151) in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... LgBiT antibody (N710A) and HiBiT antibody were acquired from Promega. Chicken polyclonal MAP2 (ab5392) ...
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bioRxiv - Molecular Biology 2021Quote: ... antibodies were detected using HRP-conjugated mouse secondary antibody (Promega). UAP56/DDX39B [1:2000] (custom generated51 ...
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bioRxiv - Molecular Biology 2021Quote: ... antibody was detected using HRP-conjugated chicken secondary antibody (Promega).
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bioRxiv - Molecular Biology 2021Quote: ... antibodies were detected using HRP-conjugated rabbit secondary antibody (Promega). Anti-Beta Tubulin III (Tuj1 ...
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bioRxiv - Genomics 2022Quote: ... Membranes were washed in TBST and probed with anti-mouse HRP secondary antibody (Promega; 1:2,000 in 5% BSA in TBST) for 1 hour at room temperature followed by development in ECL (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... three washes of 10 min in PBS-T were performed and membranes were incubated 1 hour at RT with the following 1:5000 or 1:2500 horseradish peroxidase conjugated secondary antibodies in 1% milk in PBS-T: anti-mouse (Promega, #W402B), anti-rabbit (Promega ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed thoroughly in PBS-T before incubation with secondary antibodies (anti-rabbit horseradish peroxidase, Promega WB401B 1:2,500 dilution) for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were blocked with PBS containing 5% skim milk and incubated with HRP-conjugated anti-human IgG1 antibodies (Promega, #W403B) overnight at 4 °C.
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bioRxiv - Molecular Biology 2023Quote: ... Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079) to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Molecular Biology 2023Quote: Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Membranes were washed in TBST and probed with anti-mouse HRP secondary antibody (Promega; 1:2000 in 5% BSA in TBST) for 1 h at room temperature followed by development in ECL (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed in TBST and probed with anti-mouse HRP secondary antibody (Promega; 1:5000 in 2% BSA in TBST) for 1 h at room temperature followed by development in ECL (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed 3 × 10 min in TBS-T (0.1%) before 1hr incubation in secondary antibodies (anti-rabbit HRP or AP conjugate (Promega; 1: 5.000)) ...
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bioRxiv - Cancer Biology 2020Quote: ... 4 μL of CellTiter-Glo® (Promega cat#: G7570) were added to each well and incubated for 15 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 4 mM isopropyl β-d-1-thiogalactopyranoside (IPTG; Promega) was added to each RNAi well for induction of dsRNA synthesis and plates were incubated for 3-4 hours at 30°C and 155 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... and 4 U/ml RNasin (Promega, Madison, WI, USA). Slides were then incubated with 100 nM insert/backbone oligonucleotides in PBS ...
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bioRxiv - Immunology 2021Quote: ... and 4 U/ml RNasin (Promega, Madison, WI, USA). Samples were washed and endogenous biotin was blocked using Avidin/Biotin blocking kit (Vector laboratories ...
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bioRxiv - Immunology 2021Quote: ... and 4 U/ml RNasin (Promega, Madison, WI, USA). Slides were then incubated with 100 nM insert/backbone oligonucleotides in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... We identified a common tracer probe (K-4, Promega), suitable for all mutants ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 4 µL of ONE-Glo™ Luciferase reagent (Promega) was added to each well using the BioRAPTR FRD™ ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 4 µL of ONE-Glo™ Luciferase reagent (Promega) was added to each well using the BioRAPTR FRD™ ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 μl of 4 mg/ml RNase A (Promega) was added and the sample incubated for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with 4 µL FuGene HD (Promega), 1 µg sgRNA-Cas9 plasmid DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 and exon 4 were cloned to pGL4.23 (Promega) upstream to the minimal promoter ...
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bioRxiv - Biophysics 2022Quote: ... 4% glycerol with 12 units RNasin (Promega, Madison, WI), 10 μg/ml BSA ...
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bioRxiv - Immunology 2023Quote: ... Secondary antibodies (Promega) were incubated for 1 h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was synthesized in duplicates from 100 ng total RNA using ImProm II reverse transcriptase (Promega), RNAsin (Promega ...
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bioRxiv - Microbiology 2021Quote: ... with a Luciferase Assay Reagent II and a Stop & Glo® Reagent (Promega Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Glycosylation and cysteine mutations were made with the Quick Change II Site-Directed Mutagenesis kit (Promega). A synthetic construct coding for the hemopexin sequence without any cysteines apart from cys188 was purchased from Genescript ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA synthesis was carried out using the Improm-II reverse transcriptase according to Manufacturer’s instructions (Promega). Gene expression analysis was carried out using the Brilliant SYBR Green QPCR Reagents on a Stratagene MX3000P qPCR system according to the instructions of the manufacturer and the primers described in Table 3 ...
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bioRxiv - Cell Biology 2021Quote: ... Complementary DNA (cDNA) was synthesized following manufacturer’s instructions using the ImProm II Reverse Transcriptase system (Promega) from 500 ng of total RNAs for mycelial samples and 650 ng of total RNAs for sheath samples ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNA was synthesized from purified RNA with ImProm-II™ Reverse Transcription System (Promega) and diluted 10 times for RT-qPCR to detect related genes with specific primers ...
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bioRxiv - Immunology 2021Quote: ... We then reversed transcribed RNA into cDNA by using Improm II reverse transcriptase (Promega, Madison, WI) and random hexamer primers (Genelink ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant EbfC was expressed in Escherichia coli Rosetta II cells and purified using MagneHis Particles (Promega) as previously described (24 ...
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Induction of osteogenic differentiation of MSCs by GSK3β knockdown through GSK3β siRNAs transfectionbioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was performed using the ImProm-II™ Reverse Transcription System reagent kit (Promega, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: Complementary DNA (cDNA) was synthesized from RNA using the ImProm-II Reverse Transcription System (Promega, #A3800) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... We then reversed transcribed RNA into cDNA by using Improm II reverse transcriptase (Promega, Madison, WI) and random hexamer primers (Genelink ...
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bioRxiv - Systems Biology 2023Quote: ... RNA was reverse-transcribed into cDNA using ImProm-II™ Reverse Transcription System (Promega, WI, USA). Real-time PCR was conducted using the SYBR green PCR reagent (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... Recovered RNA from all trajectories was reverse transcribed using ImProm-II Reverse Transcriptase (Promega, Madison, WI) and PCR amplified using Taq DNA polymerase for 10 cycles to generate the transcription template for the next round of selection.
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bioRxiv - Neuroscience 2024Quote: Complementary DNA (cDNA) was synthesized from RNA using the ImProm-II Reverse Transcription System (Promega, #A3800) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... the Firefly luciferase substrate (LAR II reagent, 100 µL/well, Promega Dual Glo kit, cat # E1960) was added to each well ...
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bioRxiv - Microbiology 2019Quote: ... the wells were washed and 200 µl of a 1:5,000 dilution of secondary Anti-Mouse IgG antibody conjugated to HRP (Promega Corporation, Madison, WI) in PBS + 2% BSA was added to each well ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were rinsed 5 times for 8 min in PBS-tween 0.2% and incubated with secondary antibodies for 2 h at room temperature under agitation (HRP anti-mouse, 1:10000, Promega cat. # W4021). Membranes were further rinsed 5 times for 8 min in PBS-tween 0.2% ...
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bioRxiv - Plant Biology 2021Quote: ... the protein suspensions were digested with 4 μg trypsin (Promega) in 40 μl TEAB buffer overnight at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... 25 μL of 4 mM luciferin (Assay Reagent, Promega, E1291) supplemented with isoproterenol at a final concentration of 200 nM was added ...
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bioRxiv - Microbiology 2023Quote: ... the protein suspension was digested with 4 μg trypsin (Promega) in 40 μL NH4HCO3 (50 mM ...