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Citations for Promega :
1 - 50 of 52 citations for Aminopropyl Terminated Polydimethylsiloxane Reduced Volatility since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Transcription was terminated by addition of 10 U RQ1 RNase-free DNase (Promega) and incubation at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... In vitro transcription reaction was terminated by addition of 7.5 U RQ1 Dnase (Promega) to the reaction mix and incubation for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... terminated by incubation on ice and diluted 1:5 in Glo Lysis Buffer (Promega # E2661). 25 μL of prepared Nano-Glo reagent (Promega # N1120 ...
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bioRxiv - Microbiology 2023Quote: ... The culture was terminated by adding 100 µl lysis buffer with 20 mM Tris (Promega), 5 mM EDTA (Amreso) ...
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bioRxiv - Biophysics 2021Quote: ... The assay was terminated by the addition of 25 μl of ADP-Glo™ Reagent (Promega). The 96-well plate was shaken and then incubated for 40 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... reduced and alkylated before trypsin digestion (Promega). The generated peptides were extracted with 60per centacetonitrile in 0.1per cent formic acid followed by a second extraction with 100per cent acetonitrile ...
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bioRxiv - Cancer Biology 2024Quote: ... The assay was terminated with the addition of Kinase-Glo® Luminescent Kinase Assays reagent (Promega, Cat# V6711) and read in an EnVision Multilabel Plate Reader (Perkin Elmer ...
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bioRxiv - Cell Biology 2022Quote: ... reduced with 20 mM dithiothreitol (Promega, V3155, DTT) at 95°C for 10 minutes and alkylated with 40 mM iodoacetamide (Amresco ...
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bioRxiv - Genetics 2024Quote: ... Proteins were reduced and treated with Trypsin Gold (Promega). The peptide abundance of each sample was measured and each sample was labeled with non-isobaric mass tags ...
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bioRxiv - Biophysics 2024Quote: ... 8 μl of the terminated reaction mixture were then applied to a streptavidin biotin capture membrane (SAM2, Promega, Cat.# V2861) and further treated following the manufacturer’s recommendations ...
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bioRxiv - Biophysics 2024Quote: ... The transfection was terminated 24 h later by removing the media and lysing the cells using 25 µl 1x Passive Lysis Buffer (Promega). For eIF1/eIF5 knockdown experiments ...
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bioRxiv - Evolutionary Biology 2021Quote: ... reduced and alkylated following by digestion with Trypsin Gold (Promega) and extraction of peptides as described (52) ...
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bioRxiv - Biochemistry 2024Quote: ... reduced and digested with trypsin (mass spectrometry grade; Promega Corporation) at a trypsin:protein ratio of 1:20 and peptides were purified using C18 tips (Pierce C18 Tips ...
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bioRxiv - Cell Biology 2024Quote: ... The digestion reaction was terminated by adding 200U (10 µL) SUPERase RNase inhibitor (Thermo, Cat #AM2696) and 20 µL 10% SDS (Promega, Cat #V6551) and then purified using the Zymo RNA Clean & Concentrator kit (Genesee ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were reduced and alkylated prior to digestion with recombinant LysC (Promega) and trypsin (Promega) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were reduced and alkylated prior to digestion with recombinant LysC (Promega) and trypsin (Promega) ...
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Physiological Substrates and Ontogeny-Specific Expression of the Ubiquitin Ligases MARCH1 and MARCH8bioRxiv - Immunology 2021Quote: ... Proteins were reduced and digested with sequence-grade modified Trypsin Gold (Promega). Peptides were eluted with ammonium bicarbonate and acidified peptide mixtures from each biological replicate were analyzed in technical triplicates by nanoflow reverse-phase liquid chromatography tandem mass spectrometry (LC-MS/MS ...
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bioRxiv - Cell Biology 2022Quote: ... The gel slices were reduced and in-gel digested with sequencing grade modified trypsin (Promega). The resulting samples were quenched by 10% trifluoroacetic acid and peptides were extracted and dissolved in 0.1% trifluoroacetic acid ...
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bioRxiv - Microbiology 2023Quote: ... reduced and alkylated with IAM 55 mM before digestion with 0.5 μg of Trypsin (Promega) using the FASP protocol 70 with filter passivation in 5% Tween-20 71 was used for sample preparation ...
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bioRxiv - Microbiology 2023Quote: Reduced and alkylated proteins were in-gel digested with 100ng trypsin (modified sequencing grade, Promega) overnight at 37° C ...
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bioRxiv - Microbiology 2020Quote: ... Reduced and alkylated proteins were in-gel digested with 100 ng trypsin (modified sequencing grade, Promega) overnight at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... reduced and alkylated prior to in-gel trypsin digestion employing sequence grade-modified trypsin (Promega, USA), as described in detail elsewhere (46-48) ...
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bioRxiv - Microbiology 2022Quote: ... reduced using DTT and alkylated using iodoacetamide and digested using sequencing grade Trypsin (Promega, Madison, WI). Aliquots of ca ...
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bioRxiv - Cell Biology 2024Quote: ... reduced with 10 mM DTT alkylated with 22 mM IAM digested in column with trypsin (Promega), and analyzed in a single run on the mass spectrometer.
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bioRxiv - Biochemistry 2020Quote: ... For i) Reduced and carbamidomethylated nMPO was digested in technical triplicates using sequencing-grade porcine trypsin (Promega), ii ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were reduced and alkylated followed by overnight digestion at 37°C using LysC / Trypsin proteases (Promega) and isobaric labelling using tandem mass tags (TMT ...
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bioRxiv - Microbiology 2024Quote: ... Reduced and alkylated proteins were in-gel digested with 100 ng of trypsin (modified sequencing grade, Promega) overnight at 37°C ...
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bioRxiv - Microbiology 2022Quote: Extracted DNA was quantified in 384-well plates using a reduced-volume version of the QuantiFluor (Promega) fluorescence-based assay ...
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bioRxiv - Molecular Biology 2021Quote: ... Reduced and alkylated gel pieces were processed for in-gel protein digestion by trypsin (Promega, Madison WI, USA) overnight at 37 °C adding ammonium bicarbonate buffer to cover gel matrix ...
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bioRxiv - Plant Biology 2021Quote: ... reduced (DTT) and alkylated (iodoacetamide) and subjected to enzymatic digestion with sequencing grade trypsin (Promega, Madison, WI, USA) overnight at 37°C ...
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bioRxiv - Biochemistry 2022Quote: Intracellular reduced and total glutathione levels were quantified using the bioluminescent Promega GSH-Glo™ glutathione assay (Promega), as recommended by the supplier ...
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bioRxiv - Neuroscience 2023Quote: Gel pieces were reduced (DTT) and alkylated (iodoacetamide) and subjected to enzymatic digestion with sequencing grade trypsin (Promega) overnight at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: Reduced and alkylated proteins were prepared by in-gel digesting overnight at 37°C using 100ng trypsin (Promega). The supernatant was dried by vacuum centrifugation and the samples were resuspended in 0.1% trifluoro acetic acid ...
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bioRxiv - Cancer Biology 2021Quote: Measurement of reduced to oxidized glutathione ratio (GSH/GSSG) was performed with luciferase-based GSH/GSSG-Glo assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Dried reduced/alkylated samples were then rehydrated with 20 ng/µl trypsin in 100 mM NH4HCO3 (Promega, Madison WI) on ice for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... for 4 h at 37°C and then the urea concentration was reduced to 1.5 M and digested was finalized with 1:50 (w/w) trypsin (Promega) overnight at 37°C ...
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bioRxiv - Microbiology 2024Quote: The oxidized glutathione (GSSG) and reduced glutathione (GSH) were individually measured by employing the Glutathione Assay Kit (Promega, USA) as described by Nakagawa et al ...
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bioRxiv - Physiology 2020Quote: ... reduced (using DTT) and alkylated (using iodoacetamide) and subjected to enzymatic digestion with sequencing grade Trypsin (Promega, Madison, WI, USA) overnight at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... the still not size-separated single protein band per sample was cut, reduced (50 mM DTT), alkylated (55 mm CAA, chloroacetamid) and digested overnight with trypsin (trypsin-gold, Promega).
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bioRxiv - Molecular Biology 2023Quote: Proteins bound to beads were reduced with 2 mM DTT in 2 M urea buffer and sequencing grade trypsin (Promega) was added to a final concentration of 5 ng/μl ...
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bioRxiv - Biophysics 2022Quote: ... Samples were reduced with TCEP and alkylated with chloroacetamide as previously reported.22 Reduced and alkylated samples were digested with LysC/Trypsin (Promega) followed by quenching with formic acid ...
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bioRxiv - Cell Biology 2023Quote: ... The gel slices were then reduced and alkylated followed by destaining and in-gel digestion using 125 ng Trypsin/LysC (V5072, Promega) as previously described (Shevchenko ...
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bioRxiv - Cancer Biology 2024Quote: Total glutathione and the ratio of reduced to oxidized glutathione (GSH/GSSG) were determined using the GSH-Glo Glutathione Assay from Promega, which is a luminescence-based assay ...
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bioRxiv - Biochemistry 2022Quote: ... individually reduced in situ with TCEP and alkylated in the dark with iodoacetamide prior to treatment with trypsin (Promega, sequencing grade). Each digest was analyzed by capillary HPLC-electrospray ionization tandem mass spectrometry on a Thermo Scientific Orbitrap Fusion Lumos mass spectrometer ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The urea concentration was reduced to <2M and the protein sample was digested overnight at 37°C with 1:100 Trypsin Gold (Promega V5280). The following day ...
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bioRxiv - Biochemistry 2024Quote: ... The final reduced and alkylated flow through from the column was mixed with 1 mg/mL solution of trypsin enzyme (Promega, V511A) in ratio 1:10 (w/w ...
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bioRxiv - Bioengineering 2024Quote: One hundred micrograms of each antibody were reduced with 50 mM DTT and treated with 2 µl PNGase F (Promega, USA) at 37°C for over 16h with stirring before being transferred to a new vial for LC-MS analysis ...
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bioRxiv - Neuroscience 2020Quote: The ratio of reduced (GSH) to oxidised (GSSG) glutathione was determined using a commercial assay (GSH:GSSG-Glo assay, Promega Co, Southampton, UK) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Cellular ROS was measured through the detection glutathione in its reduced (GSH) and oxidized (GSSG) forms using the luminescence-based GSH/GSSG-Glo™ Assay (Promega, V6611). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Freeze-thaw cycles were used to lyse the cells after which both reduced and oxidized glutathione were measured at 405 nm in a GloMax® spectrophotometer (Promega, Belgium) according to the manufacturer’s instructions (Glutathione Assay Kit ...