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Citations for Promega :
1 - 50 of 4792 citations for Alkaline Phosphatase Colorimetric Activity Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... alkaline phosphatase treated (Promega), gel purified and AGPAT1 was ligated into the vector using T4 DNA ligase (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... Alkaline Phosphatase development kits were purchased from Promega (S3771), and OP9 stains were performed according to manufacturer’s instructions in lightly fixed cells (4%PFA for 1 minute at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... 1.2 units alkaline phosphatase (Promega), 50 mM HEPES-NaOH (pH 8.0) ...
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bioRxiv - Genetics 2024Quote: ... alkaline phosphatase-conjugated secondary antibodies (Promega) were used.
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bioRxiv - Immunology 2023Quote: ... Streptavidin alkaline phosphatase conjugate (Promega, cat.: V5591) was diluted 1:2 500 and incubated for 1 h at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... probed with Streptavidin Alkaline-Phosphatase (Promega V5991) and detected by colorimetric assay (Promega S3841).
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bioRxiv - Developmental Biology 2024Quote: ... and added calf intestinal alkaline phosphatase (Promega) to prevent religation ...
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bioRxiv - Cell Biology 2023Quote: ... chemiluminescent substrate or alkaline phosphatase (AP)-conjugated secondary antibody and Western Blue® Stabilized Substrate for Alkaline Phosphatase (Promega). Membranes were imaged using a ChemiDoc MP imaging system (BioRad) ...
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bioRxiv - Biochemistry 2020Quote: The activity of phosphatase was determined by the Serine/Threonine Phosphatase Assay System kit from Promega Company ...
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bioRxiv - Cell Biology 2023Quote: ... incubated 3 h at 37°C and alkaline phosphatase (SEAP) activity determined measuring the absorbance at 600 nm using GloMax®-Multi Detection System (Promega).
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bioRxiv - Cell Biology 2023Quote: ... and Alkaline Phosphatase-conjugated goat anti-mouse IgG (Promega).
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bioRxiv - Biochemistry 2023Quote: ... and anti-mouse IgG alkaline phosphatase conjugated antibody (Promega). Band intensities were quantified by Image J ...
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bioRxiv - Immunology 2021Quote: PP2A activity in cell lysates was measured using the Phosphatase Assay Kit (Promega). Endogenous free phosphate was removed using the columns ...
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bioRxiv - Genomics 2022Quote: ... and 8uL Thermosensitive Alkaline Phosphatase (TSAP) in Multicore10X Buffer (Promega). Digestion was performed at 37 °C for 16 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was dephosphorylated with 8U of Thermosensitive Alkaline Phosphatase (Promega) in supplied MultiCore buffer with 80 U RNAsin for 30 minutes at 37ºC ...
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bioRxiv - Molecular Biology 2020Quote: ... or by incubation in alkaline phosphatase NBT/BICP buffer (Promega), and then quantified by densitometry analysis (Image J ...
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bioRxiv - Molecular Biology 2019Quote: ... as well as vector dephosphorylation with Shrimp Alkaline Phosphatase (Promega) and ligation with T4 DNA ligase (USB) ...
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bioRxiv - Molecular Biology 2019Quote: ... appropriate secondary antibody (1:10.000) coupled to alkaline phosphatase (Promega) was added and incubated for one hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... developed with Western Blue Stabilized Substrate for Alkaline Phosphatase (Promega) or with Super Signal West PICO Plus (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: PTP1B phosphatase activity assay was performed using a tyrosine phosphatase assay system (Promega). Endogenous phosphates were removed from the tissue extracts or cell lysates using sephadex spin column which were subsequently incubated and immunoprecipitated with an antibody targeting PTP1B ...
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bioRxiv - Molecular Biology 2021Quote: ... goat anti-rabbit immunoglobulin G conjugated with alkaline phosphatase (Promega, USA) was added to the wells and incubated for 3 h at 37 °C ...
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bioRxiv - Genetics 2020Quote: ... Alkaline phosphatase-conjugated secondary antibodies were used at 1:10000 (Promega). Western blot band intensity was analyzed with ImageJ Gel quantification tool.
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bioRxiv - Biochemistry 2024Quote: ... 80 units of calf intestinal alkaline phosphatase (Promega, Madison, WI, USA) were added and the mixture was incubated at 37 °C for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.01 U/μL Calf Intestinal Alkaline Phosphatase (CIAP, Promega, Madison, WI, USA) was added to the reaction buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... and then dephosphorylated the treated vector with TSAP (Thermosensitive Alkaline Phosphatase, Promega).
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bioRxiv - Neuroscience 2020Quote: ... Two days exposure to alkaline phosphatase (AP) substrate NBT/BCIP (Promega, S3771) was necessary to detect AP-DIG-labeled Vax1 mRNA at maximal level ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibodies used in Western blotting were conjugated to alkaline phosphatase (Promega) or horseradish peroxidase.
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bioRxiv - Cell Biology 2021Quote: ... 0.01 U/μL calf intestinal alkaline phosphatase (CIAP; Promega, Madison, WI, USA) was added to the reaction buffer ...
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bioRxiv - Neuroscience 2020Quote: ... or anti-rabbit IgG pAb conjugated alkaline phosphatase (1:10,000, S3731, Promega) were used as secondary antibodies and visualized by detecting chemiluminescence (32209 ...
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bioRxiv - Molecular Biology 2021Quote: ... 80 µl of PNK buffer containing 4U Thermosensitive Alkaline Phosphatase (TSAP) (Promega) and 2µl RNasin® (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... blots were developed with Western Blue Stabilized Substrate for Alkaline Phosphatase (Promega) and imaged using an Odyssey Fc imager (LI-COR ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies used in Western blotting were conjugated to alkaline phosphatase (Promega) or horseradish peroxidase.
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bioRxiv - Cell Biology 2023Quote: ... and treated with 2 units of calf intestinal alkaline phosphatase (Promega; M182A) at 37°C for 10 min at 1000 rpm in a Thermomixer ...
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bioRxiv - Molecular Biology 2023Quote: ... and western blue stabilized substrate for alkaline phosphatase (Promega, Madison, WI, USA) was used for chromogenic visualization of protein bands.
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bioRxiv - Molecular Biology 2023Quote: ... and western blue stabilized substrate for alkaline phosphatase (Promega, Madison, WI, USA).
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bioRxiv - Cell Biology 2021Quote: ... and then anti-mouse IgG (H&L) alkaline phosphatase-conjugated secondary antibodies (Promega) were incubated with the blots at a dilution of 1:5,000 for 1 h at room temperature (RT) ...
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bioRxiv - Cell Biology 2020Quote: Colorimetric LDH release assay kit (Promega G1780) was performed according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: Colorimetric LDH release assay kit (Promega G1780) was performed according to manufacturer’s instructions ...
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Multiple steps mediate ventricular layer attrition to form the adult mouse spinal cord central canalbioRxiv - Developmental Biology 2019Quote: ... Probe was detected using alkaline phosphatase-labelled anti-digoxigenin antibody (1:1000, Promega, 11093274910) in blocking buffer at 4°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... one of the replicate samples was treated with Calf intestinal alkaline phosphatase (Promega, M182A) for 60 min at 37°C.
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bioRxiv - Cancer Biology 2021Quote: ... 50 ng alkaline phosphatase (AP)-conjugated substrates were transfected with FuGENE HD (Promega, E2312). In KRAS related experiments ...
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bioRxiv - Molecular Biology 2019Quote: ... The beads were then dephosphorylated using the Calf Intestinal Alkaline Phosphatase (CIAP) (Promega, Germany), crosslinked RNAs were sequentially ligated to L3 and bar-coded L5 linkers (Integrated DNA technologies) ...
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bioRxiv - Systems Biology 2019Quote: ... The washes were followed by on-column TSAP incubation (Thermosensitive alkaline phosphatase, Promega, M9910) treatment for 1h at 37°C with 8 U of phosphatase in 60 μl of 1xPNK ...
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bioRxiv - Microbiology 2023Quote: ... Immunoreactive bands were detected with alkaline phosphatase conjugated secondary antibodies and NBT/BCIP (Promega). Blots shown correspond to representative results of at least 2 independent experiments ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies were visualized by using anti-rabbit immunoglobulin G-alkaline phosphatase secondary antibodies (Promega) and the CDP-star detection system (Roche Diagnostics ...
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bioRxiv - Cell Biology 2022Quote: ... anti-goat alkaline phosphatase-linked antibody and alkaline phosphatase substrate (5-bromo-4-choloro-3-indolyl 1-phosphate and nitroblue tetrazolium) from Promega (Southampton, UK); a hydroxamate-based MMP inhibitor CT-1746 (N1-[2-(S)-(3,3-dimethylbutanamidyl)]-N4-hydroxy-2-(R)-[3-(4-chlorophenyl)-propyl]-succinamide ...
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bioRxiv - Molecular Biology 2019Quote: ... The linearized plasmid was treated with 2 units of shrimp alkaline phosphatase (Promega, Madison, USA) at 37 °C for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... followed by secondary antibodies conjugated with HRP or alkaline phosphatase (Promega, Madison, WI, 1:1,000). Proteins were visualized with a LAS-3000 mini (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... followed by anti-rabbit alkaline phosphatase-conjugated secondary antibody (Promega, S3731, 1:5,000 in TBST) at room temperature for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies used included alkaline phosphatase-conjugated anti-rabbit IgG antibody (1:1,000, #S3738, Promega) and anti- mouse IgG antibody (1:1,000 ...