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Citations for Promega :
51 - 100 of 778 citations for Adenovirus Type 3 Particles Wild type since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The wild-type and mutant miRNA target sites were cloned downstream of the Firefly luciferase (luc2) reporter gene in pmirGLO vector (Promega) for luciferase reporter assays ...
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bioRxiv - Neuroscience 2021Quote: ... Streptavidin MagneSphere paramagnetic particles (Promega) were first washed in EMSA buffer 1X (EMSA buffer 2X ...
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bioRxiv - Neuroscience 2021Quote: ... Streptavidin MagneSphere paramagnetic particles (Promega) were first washed in EMSA buffer 1X four times ...
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bioRxiv - Biochemistry 2022Quote: MagneGST™ Glutathione Particles (Promega) were used for the assay ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Recombinant sodium channel expressing cell lines were generated by the transfection of wild type and F1579A mutant NaV1.4 BAC DNA constructs into HEK 293 cells (ATCC CRL-1573) by Fugene HD (Promega, Fitchburg, WI) transfection reagent according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2019Quote: ... Purification of tRNAPhe(GAA) was performed using a 5’ biotinylated complementary oligonucleotide (5’-biotin-TGGTGCCGAAACCCGGGATTGAACCGGGG-3’) coupled to Streptavidin Magnesphere Paramagnetic particles (Promega). Annealing of specific tRNA was performed in 1 x TMA buffer (Tris-HCl pH 7.5 10 mM ...
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bioRxiv - Cell Biology 2020Quote: Genomic GNA (gDNA) was extracted from each of the GFP+ and GFP-cell types using the Wizard Genomic DNA Purification Kit (Promega, Southampton, UK) and quantified using a Nanodrop 1000 Spectrophotometer (Thermo Fischer Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... The wild-type and mut-circ_0002538 fragments were cloned downstream of the luciferase reporter gene of the pMIR-Report vector (Promega, Madison, WI, USA), while wild-type and mut-PLLP 3c-UTRs were inserted downstream of the hRluc (Renilla ...
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bioRxiv - Biochemistry 2023Quote: ... Enzymatic activities of KARS wild-type and mutants were determined by measuring AMP production and ATP consumption with AMP-Glo Assay Kit (Cat#V5011, Promega, Madison, USA) and ATP Bioluminescent Assay Kit (Cat#FLAA ...
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bioRxiv - Microbiology 2022Quote: ... streptavidin MagneSphere paramagnetic particles (Promega, Z5481) were washed 3 times with 1 mL of RNA mobility buffer without heparin (5 mM HEPES pH 5.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Streptavidin MagneSphere® Paramagnetic Particles (Promega) were washed three times with Buffer A (PBS pH 8.3 ...
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bioRxiv - Cell Biology 2019Quote: ... Streptavidin MagneSphere Paramagnetic Particle beads (Promega) were used to isolated biotinylated proteins ...
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bioRxiv - Biochemistry 2021Quote: ... or mutant type (MT) and KCNQ1OT1 binding sites were synthesized and replicated onto a pGL3 Dual□luciferase Target Vector (Promega, Madison, WI, USA), to create Wild Type and Mutant Type let-7a-5p plasmids ...
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bioRxiv - Plant Biology 2022Quote: ... and MagneGST™ Glutathione Particles (Promega Cat# V861A) following the manufacturer’s manual ...
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bioRxiv - Plant Biology 2019Quote: ... Fragment-containing repeats were selected with (CT)8 and (GT)8 biotinylated oligos and recovered with streptavidin-coated magnetic particles (Streptavidin MagneSphere Paramagnetic Particles, Promega, Madison, USA). Enriched DNA fragments were amplified and cloned using the pGEM-T easy vector (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... GST-4xSUMO2 was purified using MagneGST Glutathione particles (Promega) as previously described150 ...
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bioRxiv - Pathology 2019Quote: ... Cells were then infected with adenovirus as aforementioned and cultured for additional 24 hr before lysis in Bright-Glo (Promega; catalog no. 2610). Luminescence signals were measured on a FlexStation 3 Benchtop Multi-Mode Microplate Reader (Molecular Devices ...
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bioRxiv - Biochemistry 2021Quote: 600 μL of Streptavidin-coated paramagnetic particles (Promega, Milan, Italy) were washed three times with 600 μL of PBS-1X (Euroclone ...
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bioRxiv - Microbiology 2020Quote: Magnetic beads (1μL/reaction, Streptavidine MagneSphere® Paramagnetic Particles, Promega) were washed twice in PBS and once in RB2 (20mM Tris pH7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Retroviral particles were generated using the Fugene 6 reagent (Promega) to simultaneously transfect subconfluent monolayers of 293T cells with 1μg pBABE (vector ...
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bioRxiv - Microbiology 2019Quote: ... the His-VqmA was purified using MagneHis Ni-Particles (Promega).
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bioRxiv - Bioengineering 2022Quote: ... 200 mg of streptavidin beads (streptavidin MagneSphere paramagnetic particles, Promega) were washed 3 times with 200 µL of DPBS + 0.1% BSA using a magnet (Miltenyi) ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified using MagneHis nickel particles (Promega, WI), then dialyzed against EMSA buffer (50mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... and mixed with 10 μl of MagneGST™ Glutathione Particles (Promega) for 1.5 h at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... GST or GST-OsDMI3 immobilized on the Magnet GST Particles (Promega) were incubated with His-OsMKK1/OsMKK6 in GST binding buffer (2 mM KH2PO4 ...
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bioRxiv - Biochemistry 2020Quote: ... biotinylated RBD-T was immobilized on the Streptavidin MagneSphere particles (Promega) and added to the solution containing phage ...
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bioRxiv - Microbiology 2022Quote: ... The clarified soluble protein suspension was purified using MagneGST Glutathione Particles (Promega) according to manufacturer recommendations ...
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bioRxiv - Biophysics 2024Quote: Lentiviral particles were produced in HEK293T cells using Fugene HD (Promega, #E2311) transfection reagent following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... 1 % Triton-X100) was added to the reactions with MagneGST Glutathione Particles (Promega) in the presence/absence of 10 mM of the indicated sugars ...
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bioRxiv - Biochemistry 2020Quote: ... magnetic beads were prepared by taking 100 μL of Streptavidin Magnesphere Paramagnetic Particles (Promega) and incubated with 5 μM of Spike-Fc-AviTag for 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... GST-tagged AtCFI59 was purified using agarose beads (MagneGSTTM Glutathione Particles; Promega, Madison, USA) and retained on the beads to pull-down purified HIS-tagged AtCFI25a protein in TBS buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... After 3 days Caspase-Glo 3/7 (Promega) and CellTiterGlo (Promega ...
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bioRxiv - Biochemistry 2023Quote: ... The efficiency of biotinylation was subsequently evaluated by capture using streptavidin-coated paramagnetic particles (Promega). The first round of selection was performed using 400 nM of nanodisc-reconstituted MmFLVCR2 diluted in selection buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... 5’-CGCCTTCGTTACAAGCATCG-3’; antisense, 5’-AAGAGCAAACGCATCTCCGA-3’), GAPDH (sense, 5’-ACCCACTCCTCCACCTTTGAC-3’; antisense, 5’-CTGTTGCTGTAGCCAAATTCGT-3’) using Green GoTaq master mix (Promega, Madison, WI) with C1000 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... GST fusion proteins were isolated by binding to glutathione magnetic beads (MagneGST™ Glutathione Particles, Promega) for 30 min at room temperature on a rotating wheel ...
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bioRxiv - Cancer Biology 2019Quote: Lentiviral or retroviral particles were produced by transient calcium phosphate transfection (ProFection mammalian transfection System; Promega) of 293T and Phoenix cells ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant EbfC was expressed in Escherichia coli Rosetta II cells and purified using MagneHis Particles (Promega) as previously described (24 ...
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bioRxiv - Plant Biology 2022Quote: ... and GST- GBF3 were purified from 4 ml culture and immobilized on MagneGST Glutathione particles (Promega). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... GSK-3 (Promega), CK1 (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: ... the two blots were hybridized O/N at 65 °C to each their radioactively labeled 3’ CF probe (AGO1 3’ CF and CSD2 3’ CF probes labelled with Prime-a-gene labeling kit from Promega). The membranes were washed 3 times in 2xSSC (0.3 M NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... The remainder of each extract was added to 40 μl magnetic nickel beads (MagneHis™ Ni-Particles, Promega). Beads were rotated for 2 h at room temperature to purify ubiquitinated proteins and washed once with lysis buffer B supplemented with 20 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Viral particles were generated by transient transfection of each expression vector into HEK 293T cells using Fugene6 (Promega) with the PCL-10A1 packaging system according to the manufacturer’s recommended protocol ...
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bioRxiv - Plant Biology 2022Quote: ... His-tagged and GST-tagged recombinant proteins were purified using Promega MagneHis™ Ni-Particles (Promega Cat#V854A) and MagneGST™ Glutathione Particles (Promega Cat# V861A ...
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bioRxiv - Cancer Biology 2020Quote: ... Generation of infectious lentiviral particles and subsequent cell transduction was performed as previously described.26 FuGENE 6 (Promega) was used to transfect select plasmids ...
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bioRxiv - Biophysics 2019Quote: ... Proteasomes were affinity purified with the bait protein GST-Ubl and magnetic beads (MagneGST™ Glutathione Particles, Promega) and eluted with purification buffer containing 25 mM reduced L-glutathione ...
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bioRxiv - Microbiology 2023Quote: Lentiviral particles were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Caspase-Glo 3/7 Reagent (Caspase-Glo® 3/7 Assay, Promega) was added to the wells ...
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bioRxiv - Cancer Biology 2022Quote: ... Caspase 3/7 activity was detected using Caspase-Glo 3/7 (Promega). Annexin V/Propidium Iodide staining was performed using Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... caspase-3/7 levels were examined using Caspase-Glo 3/7 (Promega) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2023Quote: High molecular weight DNA was isolated from spleen tissue of a single male from each of the 11 imported Nachman wild-derived inbred strains using the Wizard DNA Purification Kit (Promega) or the Monarch HMW DNA kit (NEB ...