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Citations for Promega :
251 - 300 of 1231 citations for 8 Fluoro quinazoline 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The following day cells were transfected with plasmids encoding for FLAG-tagged NLRP1 (2 μg) or the indicated DPP9 construct (2 µg) with FuGENE HD according to manufacturer’s instructions (Promega). Cells were harvested ...
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bioRxiv - Molecular Biology 2023Quote: ... precipitated proteins were eluted by incubation of beads with Elution Buffer (2 M Urea, 50 mM Tris pH 7.5, 2 mM DTT, 20 µg/ml Trypsin Gold (Promega)) for 30 min at 37°C shaking at 1,400 rpm in the dark ...
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bioRxiv - Cancer Biology 2022Quote: ... Metabolic activity was measured at day 4 with CellTiter 96 (Promega) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... Then 4 µL/well of Bright-Glo Luciferase detection reagent (Promega) was added to assay plates and incubated for 5 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Then 4 μL/well of Bright-Glo luciferase detection reagent (Promega) was added to assay plates and incubated for 5 min at room temperature ...
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bioRxiv - Physiology 2021Quote: ... and overnight protein digestion using 4 μg MS-grade trypsin (Promega) at 37°C ...
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bioRxiv - Immunology 2020Quote: ... by using 4 μL of FuGENE HD transfection reagent (Promega #E2311) according manufactured protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Then 4 μL/well of Bright-Glo Luciferase detection reagent (Promega) was added to assay plates and incubated for 5 min at room temperature ...
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bioRxiv - Genomics 2019Quote: RNA samples (4 ug) were mixed with random hexamers (Promega, #C118A) and heated at 70 °C for 5 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell death was measured after 4 days using CytoTox Glo (Promega).
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bioRxiv - Microbiology 2019Quote: Cells were harvested in 4 M guanidine thiocyanate (Promega, United States) in a 1% sodium lauryl sarcosine (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Resin was then washed 4 times with Resin Wash Buffer (Promega). Complexed proteins were eluted in SDS Elution Buffer (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... and overnight ligation at 4°C (T4 DNA ligase, Promega, USA). Ligated chimeras were transformed into DH5α (ThermoScientific ...
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bioRxiv - Developmental Biology 2023Quote: ... 100 nM dT-primer and 4 U RNase Inhibitor (Promega, N2611). Reverse transcription was performed at 42°C for 90 min after filling up to 10 μl with RT buffer mix for a final concentration of 1x superscript II buffer (Invitrogen) ...
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Caspase-8 Modulates Angiogenesis By Regulating A Cell Death Independent Pathway In Endothelial CellsbioRxiv - Developmental Biology 2019Quote: The Caspase-8 Glo® assay was performed in combination with the CellTiter-Fluor™ Cell Viability Assay (both from Promega) according to the manual ...
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bioRxiv - Cell Biology 2020Quote: ... with a Tris/NaCl buffer (100 mM Tris and 75 mM NaCl at pH 8) containing 1 μg LysC (Promega, Va11A) per sample ...
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bioRxiv - Cell Biology 2021Quote: ... 30 min) and diluted to 1M urea with 200mM ammonium bicarbonate for trypsin digestion (1μg, 37°C, 8 h, Promega cat # V5113).
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bioRxiv - Molecular Biology 2022Quote: ... samples were diluted 8 times with 0.1M ammonium bicarbonate and sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) was added ...
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bioRxiv - Neuroscience 2022Quote: To assess the effect of DA and DA-HA on cell viability, CCK-8 (DJDB4000X, Dojindo laboratory) or MTT (CellTiter 96® Non-Radioactive Cell Proliferation Assay, Promega) was used ...
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bioRxiv - Microbiology 2022Quote: Activity of caspases-3/7 and -8 were assessed using the corresponding Caspase-Glo® Assays in white-walled 96-well plates (Promega).
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bioRxiv - Neuroscience 2023Quote: ... was performed by incubating the proteins first with endoproteinase Lys-C (Wako, #125-05061,)) in 8 M urea for 4h at 37°C followed by an overnight trypsin digestion (Promega #V511A,) in 2 M urea at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then resuspended in 30 μL of 8 M Urea and 30 μL of ProteaseMax surfactant (20 μg/mL in 100 mM ammonium bicarbonate, Promega, V2071).
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bioRxiv - Biochemistry 2021Quote: ... Genomic DNA was eliminated by incubating 2 μg of RNA with 2 U of RQ1 RNase-free DNase I (Promega) for 30 min at 30°C ...
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bioRxiv - Molecular Biology 2021Quote: ... FASP was carried out in two 10 kDa MWCO filters with a 50 mM iodoacetamide alkylation step and proteins were digested in 2M urea with 2% wt/wt Lys-C (Wako) for 6 h and 2% modified trypsin (Promega) for 12 h at 37°C ...
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bioRxiv - Genetics 2022Quote: ... Vectors coding for repair template DNA of roughly 2 kb were generated from Drosophila Schneider 2 cell genomic DNA by PCR using GoTaq G2 Polymerase (Promega) and primer pairs appropriate for the desired region (Supplementary Table S1) ...
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bioRxiv - Systems Biology 2020Quote: ... Reduced proteins were alkylated and trypsin-digested for 1-2 hours in trypsin solution (2 mg/ml sequencing grade porcine trypsin (Promega)/100 mM Tris/10 mM iodoacetamide ...
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bioRxiv - Microbiology 2022Quote: 293T cells were co-transfected with pEGFP-N1 and plasmids expressing SARS-CoV-2 Spike (FL) or SARS-CoV-2 Spike-Δ19 for 24 h using FugeneHD (Promega). The cells were treated with DMSO or 10 µM 6-TG at 4 h post- transfection ...
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bioRxiv - Biophysics 2019Quote: ... cells were treated with the LRRK2 inhibitor MLi-2 (100 nM) for 2 hours and then labelled with the TMR ligand (3 μM)(Promega). After 15 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were diluted to a concentration of urea of 2 mol L-1 and digested overnight with the addition of 2 µl sequencing-grade trypsin (Promega). After digestion ...
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bioRxiv - Biochemistry 2022Quote: ... 3.5 mM MgCl2, and 2 mM ATP (for nucleic acid substrates containing RNA, 2 U/μl of RNasin Ribonuclease Inhibitor (Promega)) for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: Proteins bound to beads were reduced with 2 mM DTT in 2 M urea buffer and sequencing grade trypsin (Promega) was added to a final concentration of 5 ng/μl ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then digested overnight with 2 µg of lysyl endopeptidase (Wako) at room temperature and further digested overnight with 2 µg trypsin (Promega) at room temperature ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... The pellet was washed with 2 ml nuclei storage buffer and resuspended in 0.2 ml PBS (calcium and magnesium-free) with 2% BSA and 0.2 U/μl RNasin® Plus RNase inhibitor (Promega). Buffer composition was described before72 ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested with 2 μg of lysyl endopeptidase (Wako) at RT for overnight and were further digested overnight with 2 μg trypsin (Promega) at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µl RNasin Plus (Promega, catalog no. N2615), 0.8 µl 100 mM ATP (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... using 2 µg of sequencing grade trypsin (Promega) per fraction 55 ...
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bioRxiv - Biochemistry 2022Quote: ... either 2 µg pGL4.47[luc2P/SIE/Hygro] (Promega) or pGL4.52[luc2P/STAT5RE/Hygro] (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... t-ERK1/2 (Promega V114A, 1/1000 dilution), p-AKT (Cell Signaling Technology ...
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bioRxiv - Immunology 2019Quote: ... with 2 µM Oligo(dT)15 primers (Promega) and 10 U RNase OUT in a 20 µl reaction at 37ºC for 90 min ...
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bioRxiv - Neuroscience 2019Quote: ... and 2 μg of sequence-grade trypsin (Promega). Samples were digested overnight in a 37 °C thermomixer (Eppendorf) ...
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bioRxiv - Molecular Biology 2021Quote: ... 2) pRL Renilla luciferase control reporter (Promega, E2241) and 3 ...
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bioRxiv - Cell Biology 2019Quote: ... 2° antibodies α- mouse (HRP detection, Promega, W4021). The mean intensity quantification of 4 independent experiments is shown in Figure 4 – supplement 1B ...
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bioRxiv - Cancer Biology 2021Quote: ... pGL4.10 (Luc.2; E665A) was purchased from Promega. The pGL4.10-ITGB1 reporter and a control Renilla luciferase vector were co-transfected into breast cancer cell lines using Lipofectamine 3000 transfection kit (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... plus 2 uL of random primers (Promega, C1181). This was then incubated at 70 °C for 10 mins ...
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bioRxiv - Biochemistry 2022Quote: ... 2 U/μl of RNasin Ribonuclease Inhibitor (Promega)) for 20 min on ice ...
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bioRxiv - Microbiology 2022Quote: ... 2 µM dNTPs and MMLV reverse transcriptase (Promega). Primer extension reactions to analyze initiation from the +1 and +3 sites of the le promoter were subjected to electrophoresis in 6% polyacrylamide gels containing 7 M urea in Tris-borate-EDTA buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µg of sequencing grade modified trypsin (Promega) was added ...
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bioRxiv - Systems Biology 2023Quote: ... 2 µl of 5x MTase-Glo Reagent (Promega) was added to wells ...
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bioRxiv - Cancer Biology 2023Quote: ... we used CellTiter Glo 2 assay systems (Promega). Approximately 2.0 × 103 cells/well were seeded in a 96-well plate and incubated in RPMI1640 culture medium over 4 days at 37 °C and 5% CO2 ...
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bioRxiv - Biophysics 2022Quote: ... 2 μg trypsin (protein:enzyme ratio 50:1; Promega) were added and proteins were digested at 37 °C for 18 h ...