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Citations for Promega :
401 - 450 of 686 citations for 8 6 AMINOHEXYL AMINOADENOSINE5 TRIPHOS PHATE LITH since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Equilibrated cells were transfected with 0.8 μg of 5-HT1eR or 5-HT1FR and 8 μg of GloSensor plasmid (Promega), after mixing with 17.6 μl of PEI in OptiMEM (Gibco) ...
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bioRxiv - Plant Biology 2020Quote: ... following the manufacturer’s instructions (eluted into 8 μl of TLE) before A-tailing in a reaction containing 2 μl of GoTaq buffer (Promega), 0.2 μl 10 mM ATP ...
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bioRxiv - Microbiology 2020Quote: ... post-transfection with or without OSM (10 ng/mL for 8 h) or IFN-α (1,000 U/ml for 16 hours) before lysis using Passive Lysis Buffer (Promega). Firefly and Renilla luciferase activity was then determined in a dual luciferase assay ...
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bioRxiv - Neuroscience 2020Quote: ... 8 min, 4 °C) and resuspended in staining buffer (PBS, pH 7.4, with 0.5% RNase-free BSA (Promega, Heidelberg, Germany) and 0.2 U/μl of RNasin Plus RNase inhibitor (Promega)) ...
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bioRxiv - Biophysics 2020Quote: ... as well as expression construct of interest in a 9:8:1 mass ratio into HEK293T cells using FuGENE HD Transfection Reagent (Promega) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... The bound proteins were digested by resuspending the beads in 200 μL freshly prepared digestion buffer (2M Urea, 100 mM Tris-HCl pH 8, 100 mM DTT) with 0.5 ug trypsin (Promega, V5111) and overnight incubation at room temperature while shaking ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then transfected with plasmids DNA (250 ng per plasmid per well) using FuGENE HD (Promega, 1:8 ratio), and cultured at 28°C ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 4 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:250 (w/w ...
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bioRxiv - Immunology 2019Quote: 8 nM cap0 or 20 ng/μL VPg-linked RNA was translated using the Flexi Rabbit Reticulocyte Lysate system (Promega) in the presence or absence of 1.5 μM Ifit proteins ...
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bioRxiv - Bioengineering 2019Quote: ... and 8-oxo-2’-deoxyguanosine-5’-triphosphate (8-oxo-dGTP) respectively,52 both in combination with the non-proofreading Taq DNA polymerase (GoTaq, Promega). The primers used for this PCR reaction anneal outside the His6-SpAS1 open reading frame (Table S8) ...
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bioRxiv - Molecular Biology 2020Quote: ... RL and FL activities were measured on 96-well plates using 2 µl of cell extract containing 8 µl of 1x PLB (Promega) and the dual-luciferase assay kit (Promega) ...
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bioRxiv - Systems Biology 2021Quote: ... In 18 hours cells were stimulated for 8 hours with a corresponding inducer and harvested in the passive lysis buffer (Promega). Luminescence of Firefly and Renilla luciferases was measured using dual-luciferase-reporter assay (Promega ...
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bioRxiv - Immunology 2019Quote: ... Samples were diluted 10-fold with 50 mM Triethylammonium bicarbonate buffer at pH 8 and incubated overnight at 37°C with sequencing grade trypsin (Promega) added at a 1:50 enzyme:substrate ratio (wt/wt).
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bioRxiv - Cell Biology 2019Quote: ... Samples were diluted 10-fold with 50 mM triethylammonium bicarbonate buffer at pH 8 and incubated overnight at 37°C with sequencing grade trypsin (Promega) at a 1:50 enzyme:substrate ratio (wt/wt).
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were prepared by addition of 0.3N hydrochloric acid then 450nM Tris (pH=8) and metabolites were analyzed using the Glutamine/Glutamate Glo Assay (Promega; J8021) per manufacturer’s instruction.
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bioRxiv - Molecular Biology 2021Quote: ... the coding sequence of REST/NRSF ZF1-8 were amplified by PCR using cDNA as templates and cloned into the pTNT vector (Promega) between the EcoRI and XbaI sites ...
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bioRxiv - Immunology 2021Quote: ... cells were transfected with one of the Gag-mCherry variants (8 µg) using FuGene HD according to the manufacturer’s instructions (Promega, USA) in a 3:1 ratio with DNA ...
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bioRxiv - Genomics 2021Quote: ... 8 μg of RNA were treated for 1 h at 37°C with 12 units of RQ1 DNase (Promega®) followed by phenol-chloroform extraction and ethanol precipitation of RNA which was subsequently dissolved in water ...
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bioRxiv - Developmental Biology 2022Quote: ... Luciferase assays were conducted 8 hours after stimulation using a dual luciferase kit (Dual-Luciferase® Reporter Assay System, Promega) according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: ... the absorbance was measured at 450 nm (CCK-8) or 560 nm (MTT) using a microplate reader (GloMax explorer multimode, Promega).
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bioRxiv - Biochemistry 2022Quote: ... Luciferase expression was measured at 8 hr (for translation) and 72 hr (for replication) using the Renilla Luciferase Assay System (Promega). Renilla luciferase luminescence was measured using a Clariostar Microplate Reader (BMG Labtech ...
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bioRxiv - Cell Biology 2022Quote: ... and the -1680 bp to +8 bp and -1525/-1495 element mutant were constructed in the pGL3-Basic luciferase reporter vector (Promega) using standard molecular biology techniques ...
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bioRxiv - Neuroscience 2022Quote: ... Digestion of proteins in the gel pieces was done by covering them with a trypsin solution containing (8 ng/µL sequencing-grade trypsin (Promega), dissolved in 50 mM NH4HCO3with 10% ACN) ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR Tag analysis was performed either by adding 1.8 μL of yeast lysate used as template DNA to 6.25 μL of 2X GoTaq® Green Master Mix (Promega), 4.75 μL of nuclease-free water and 400 nM of each primer or by adding 1 µl of yeast lysate to 5 µl of DreamTaq Green PCR Master Mix (2X ...
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bioRxiv - Immunology 2022Quote: ... Ectopic Flag-tagged Galectin 8 or HA-tagged LILRB4 plasmid were transfected into the Mc38/B16/HEK294 cells with Fugene (Promega). A blank vector control was applied ...
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bioRxiv - Cancer Biology 2022Quote: ... CaspaseGlo assays were performed in white-walled 96-well plates to measure the activity of caspase 9 (cat. G8210) or caspase 8 (cat. G8200) (both from Promega) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The AAV production media were collected 72 h and 120 h post transfection and concentrated using a PEG-precipitation method with 8% PEG-8000 (wt/vol; Cat# V3011, Promega). The harvested cell pellets were re-suspended and lysed through sonication ...
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bioRxiv - Microbiology 2023Quote: ... In solution digests were performed overnight at pH 8 and 37 °C with mass spectrometry grade trypsin (Promega, Madison, WI) at a 1:33 enzyme-to-protein ratio and quenched with formic acid (0.15%) ...
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bioRxiv - Microbiology 2023Quote: ... Viral titers were measured by infecting MDCK cells and measuring Nanoluciferase activity 8 h later with the Nano-Glo Luciferase Assay System (Promega)(52).
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bioRxiv - Biophysics 2024Quote: ... 8 μl of the terminated reaction mixture were then applied to a streptavidin biotin capture membrane (SAM2, Promega, Cat.# V2861) and further treated following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2024Quote: ... To quantify the activity of reports groups of 5 embryos were collected 8 hours post-injection in triplicates and lysed in 100 mL of lysis buffer (Promega). Reporter expression was quantified with the Nano-Glo Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmid transfection of U2OS cells with PIF1 variants was carried out using FuGENE 6 Transfection Reagent (Promega) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: HeLa cells were plated at 100,000cells in 2 ml media onto 35mm MatTek glass bottom dishes and transfected with 0.9µg RUSH plasmid and 0.1µg eGFP-GalT or LAMP1-GFP using 100µl Opti-MEM and 3µl Fugene 6 transfection reagent (Promega) one day before imaging ...
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bioRxiv - Molecular Biology 2019Quote: ... Instrument quality control was monitored using the Promega 6×5 LC-MS/MS Peptide Reference Mix (Promega) before and after each MS experiment run ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were washed again and 6 µg of plasmid DNA was added with FugeneHD transfection reagent (Promega). Supernatants were collected at 24 ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated for 6 h at 37°C with 3 μg Sequencing Grade Modified Trypsin (Promega). Digestion was terminated via addition of 20 μL trifluoroacetic acid (TFA) ...
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bioRxiv - Developmental Biology 2020Quote: ... 6 uL of RNA was used for each cDNA synthesis (GoScript™ Reverse Transcription System kit, Promega). qPCR was performed using Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... following manufacturer’s guidelines and a ratio of 0.5 µg of plasmid:1.5 µL FuGene 6 (Promega, E2691) in Opti-MEM (Gibco) ...
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bioRxiv - Biochemistry 2020Quote: ... HeLa cells were transiently transfected with the PX459–sgRNF213-exon3 vector using FuGENE 6 Transfection Reagent (Promega). Forty-eight hours post-transfection ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection was done on pre-seeded HeLa cells (70% confluency) with the FuGENE 6 Transfection Reagent (Promega) following standard protocol ...
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bioRxiv - Cell Biology 2022Quote: eGFP-HSP27 immunoprecipitations were performed in p62KO HeLa cells 24hrs following transient transfection using Fugene 6 (Promega). HeLa cells were treated with 750µM LLOMe for 2hrs prior to immunoprecipitation ...
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bioRxiv - Neuroscience 2022Quote: ... mouse Piezo2 (Lewis et al., 2017): 2.5 µg + 0.5 µg YFP) with Fugene 6 (Promega, Madison, WI) 48 hours prior to recording at a transfection ratio of 10 µL Fugene6:3 µg total DNA ...
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bioRxiv - Microbiology 2023Quote: ... and 0.25 μg vesicular stomatitis virus G glycoprotein expression plasmids using the Fugene 6 transfection reagent (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... and 500 ng of pMCB306 plasmids described above along with 3 µl of Fugene 6 (E2692, Promega) transfection reagent ...
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bioRxiv - Developmental Biology 2022Quote: ... in 6-well plates with pActin-Gal4 and pUAS-Lgr4 vector using Fugene-HD (Cat. # E2311, Promega). Twenty-four hours after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... and then they were transfected with the expression plasmid DNAs by using FuGENE 6 (Promega, Madison, WI).
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bioRxiv - Cancer Biology 2023Quote: ... into HEK293T cells in 57 µL Opti-MEM and 6 µL FuGENE HD (Promega, Fitchburg, WI, USA) transfection reagent as described (Gerstmeier et al ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured at day 4 and day 6 using the Cell-Titer Glo assay (Promega). Viability data was analyzed by comparing the relative viability change between base editing with the uORF gRNA and the associated parental CDS gRNA ...
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bioRxiv - Biochemistry 2023Quote: ... 2-4μg bacmid was transfected into Sf9 cells in 6 well plates using Insect-XPRESS (Promega E2311). Cells were incubated at 2°C for 5 days and bacmid YFP expression assessed by Leica DM IL LED Fluo microscope using a green fluorescent protein filter cube ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... FuGene 6 (cat#: E2692) transfection reagent and sequencing grade modified trypsin (cat#: V5111) were purchased from Promega. Cas9 endonuclease was purchased from Integrated DNA Technologies.