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Citations for Promega :
101 - 150 of 4294 citations for 6H dibenz C E 1 2 oxaphosphorin 6 oxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... To determine the optimum transfection ratio multiple complexes were created (1.5:1, 3:1, 6:1) using ViaFect Transfection reagent (Promega, E4981) and pGL4.51 [luc2/CMV/Neo] plasmid vector (Promega ...
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bioRxiv - Immunology 2023Quote: ... COS-1 cells were transfected with pBRmac239 proviral DNA using FuGENE 6 (Promega). At 48 h post-transfection ...
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bioRxiv - Cell Biology 2023Quote: ... 30 μL of CellTiter-Glo reagent solution (Promega, diluted 1:6 in water) was added to each well after 24 h and 72 h of compound treatment ...
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bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 spike protein (23) into HEK 293T cells using the FuGENE 6 Transfection Reagent (Promega). Spike sequences from the following global strains REF were used ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with 2 μg of FLAG-tagged β2 or β1 adrenergic receptor DNA using Fugene 6 (Promega) and seeded at a density of ∼70,000 cells.ml−1 on 18 mm glass coverslips 4-8 hours after transfection.
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bioRxiv - Cell Biology 2022Quote: ... were transfected with 2 μg of fresh bacmid DNA and 6 μL of FuGene HD transfection reagent (Promega, E2311) according to the manufacture’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM CaCl2 and 500 ng Trypsin Lys-C (Promega). On bead digest was carried out overnight at 37°C while shaking at 200 rpm ...
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bioRxiv - Molecular Biology 2022Quote: ... firstly with Endoproteinase Lys-C (ratio 1:33 enzyme:lysate) (Promega) for 1 hour at room temperature then with trypsin (ratio 1:33 enzyme:lysate ...
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bioRxiv - Molecular Biology 2021Quote: ... thiopropyl sepharose beads were incubated overnight at 37°C with 2 μg trypsin Gold (Promega, #V5280) in 50 mM NH4HCO3 ...
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bioRxiv - Plant Biology 2020Quote: ... were digested for 12 hr at 37 °C with 2 mg of sequencing-grade trypsin (Promega), followed by a second incubation for 6 hr with an additional 2 mg of trypsin ...
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bioRxiv - Genetics 2022Quote: ... The lysate was digested overnight at 37 °C with 2 μg of sequencing-grade trypsin (Promega), after which the sample was acidified to 0.1% formic acid ...
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bioRxiv - Microbiology 2020Quote: ... or Vpr F34I/P35N mutant using 6 μl Fugene-6 (Promega). For KPNA-cargo IPs HEK293T cells were grown in 10 cm dishes and co-transfected with 1 μg of a plasmid expressing FLAG-tagged KPNA1 ...
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bioRxiv - Systems Biology 2021Quote: ... Protein digestion was performed for 16 h at 37 °C with 0.1 µg of Trypsin/Lys-C mix (1 µL of stock; Promega) and stopped by lowering pH to 3 by the addition of 5 µL of 10% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... at a protein:Lys-C ratio of 100:1 (w/w) for 4 h at 37°C followed by trypsin (Promega) digestion at a ratio of 50:1 (w/w ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were digested for 2 h at RT using 0.5 μg Lys-C (Wako Chemicals) and for 16 h at 37 °C using 1 μg trypsin (Promega). After centrifugation (10 min at 14 000 g) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were proteolysed for 2 h at room temperature using 0.5 μg Lys-C (Wako) and subsequently for 16 h at 37 °C using 1 μg trypsin (Promega). Peptides were collected by centrifugation and acidified with 0.5 % trifluoroacetic acid.
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bioRxiv - Genetics 2020Quote: ... Competent Escherichia coli cells (E. coli JM109; Promega, Fitchburg, USA) were incubated with the ligation mixture on ice for 30 min and then transformed by a 45 sec heat shock at 42 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Plat-E cells were transfected with FuGENE™ HD (Promega) and transfection regent was incubated overnight before a media change ...
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bioRxiv - Microbiology 2020Quote: ... or pNL-Luc2-E--IN/HiBiT-Fin using FuGENE6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... Plat-E cells were transfected with FuGENE™ HD (Promega) and transfection regent was incubated overnight before a media change ...
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bioRxiv - Cell Biology 2019Quote: Glucose uptake was measured based on the detection of 2-deoxyglucose-6-phosphate uptake by a commercially available luminescence-based kit (Glucose Uptake-GloTM Assay, Promega) on a SpectraMax M3 (Molecular Devices) ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293T cells were grown in two 175 cm2 flasks to ∼50% confluence and transfected with 25 μg plasmid and 75 μl FuGENE 6 in 2 ml Opti-MEM according to the manufacturers instructions (Promega). One day after transfection ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293T cells and C3H10T1/2 cells were transfected in 6-well plates with various constructs of pmirGlo vector (Promega®). Transfection was performed using Turbofect transfection reagent (Thermo Fischer scientific® ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV-2-spike (kindly provided by Dr. Peihui Wang, Shandong University, China) using FuGENE 6 (Promega). Cells were cultured overnight at 37 °C with 5% CO2 ...
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bioRxiv - Cell Biology 2019Quote: Desired protein amounts were aliquoted and chloroform methanol precipitated, followed by digestion with LysC (overnight at room temperature, vortex speed 2; Wako) and trypsin (6 hours, 37° Promega) digestion ...
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bioRxiv - Molecular Biology 2021Quote: A total of 10 μg of bacmid DNA was transfected into 6 wells of 2×106 adherent Sf9 cells (at 5×105 cells/ml) using the transfection reagent Fugene HD (Promega). 42–72 h post transfection ...
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bioRxiv - Immunology 2021Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV-2-spike (kindly provided by Dr. Peihui Wang, Shandong University, China) using FuGENE 6 (Promega). Cells were cultured overnight at 37°C with 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... 1 × 106 Sf9 cells in 2 mL of media in a 6-well dish were transfected with up to 2 µg of fresh bacmid DNA using FuGene HD transfection reagent (Promega) at a ratio of 3:1 (Fugene reagent:DNA ...
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bioRxiv - Immunology 2023Quote: ... and the corresponding SARS-CoV-2 spike construct were co-transfected in HEK293-T cells using FuGENE 6 Transfection Reagent (Promega) in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Plant Biology 2020Quote: ... approximately 0.6 mg IgG-enriched proteins were first digested on the beads with mass spectrometry-grade Trypsin/Lys- C Mix (Promega, Madison, WI, USA) at a 1:50 (enzyme/substrate ...
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bioRxiv - Microbiology 2020Quote: ... HeLa cells were transfected 6 hours before imaging with Fugene 6 (Promega) and 1000ng of DNA ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of both plasmids was mixed with 6 μl FuGENE reagent (Promega E269A) in 200 μl serum free DMEM and incubated for 30 minutes before adding the transfection mix to the cells ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of both plasmids was mixed with 6 μl FuGENE reagent (Promega E269A) in 200 μl serum free DMEM and incubated for 30 min before adding the transfection mix to the cells ...
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bioRxiv - Cancer Biology 2023Quote: ... for 1 hour at 37 °C then with Trypsin (ratio 1:33 enzyme: lysate, Promega) overnight at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... using Fugene 6 (Promega). Supernatant was harvested from the transfected cells for the next three days and then centrifuged ...
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bioRxiv - Cell Biology 2020Quote: ... using Fugene 6 (Promega), with a linearized TCIS construct described previously6 ...
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bioRxiv - Genetics 2021Quote: ... using FuGENE 6 (Promega) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... using FuGENE 6 (Promega). Sixteen hours later ...
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bioRxiv - Cancer Biology 2020Quote: Transfection: Fugene 6 (Promega); TransIT-2020 (Mirus Bio) ...
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bioRxiv - Biophysics 2020Quote: ... FuGENE 6 (Promega, E2691) was used for transient transfection according to the manufacturer’s instructions.
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bioRxiv - Genetics 2019Quote: ... using FuGENE 6 (Promega) on day 0 ...
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bioRxiv - Developmental Biology 2022Quote: ... or Fugene 6 (Promega) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... using FuGENE 6 (Promega). Cells were cultured overnight at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... using Fugene 6 (Promega).
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bioRxiv - Microbiology 2020Quote: ... using FuGENE 6 (Promega). Sixteen hours later ...
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bioRxiv - Cell Biology 2022Quote: ... or FuGENE 6 (Promega) following the manufacturer’s protocol with modifications (see below).
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bioRxiv - Microbiology 2022Quote: ... or Fugene 6 (Promega) and supernatants harvested 3 days later ...
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bioRxiv - Cell Biology 2023Quote: ... utilizing Fugene 6 (Promega). Culture supernatants were collected at 48h and 72h post-transfection ...
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bioRxiv - Cell Biology 2022Quote: ... t-ERK1/2 (Promega V114A, 1/1000 dilution), p-AKT (Cell Signaling Technology ...
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bioRxiv - Biophysics 2022Quote: ... 2 μg trypsin (protein:enzyme ratio 50:1; Promega) were added and proteins were digested at 37 °C for 18 h ...