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Citations for Promega :
501 - 550 of 4525 citations for 6H Imidazo 4 5 e 2 1 3 benzothiadiazole 7 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay Virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system (RNA1 qPCR primers GW194 and GW195)23.
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bioRxiv - Biochemistry 2021Quote: ... treated with 3 mM dithiothreitol (DTT) (molecular grade, Cat. 3483-12-3, Promega), and incubated for 30 min at 37°C in 5% CO2 in serum-free RPMI media ...
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bioRxiv - Microbiology 2022Quote: ... the virus-induced cytopathogenic effect was measured colorimetrically by the formazan-based 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cell viability assay (CellTiter 96 AQueous One Solution Cell Proliferation Assay from Promega, Madison, WI), and the antiviral activity was expressed as the 50% effective concentration (EC50) ...
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bioRxiv - Cancer Biology 2019Quote: ... The cell viability was assessed by the viability assays 3-(4,5-dimetiltiazol-2-il)-5-(3-carboximetoxifenil)-2-(4-sulfofenil)-2H-tetrazolio (MTS) using the Cell-Titer 96c AQueous Non-Radioactive Cell Proliferation Assay kit (Promega, Madison, USA) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... 3,500,000 cells were seeded in 10 cm plates (2-4 per replicate) and transfected with FuGENE® 6 Transfection Reagent (Promega, E2692). In one tube ...
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bioRxiv - Microbiology 2022Quote: Various KSHV mRNA 5’-UTRs containing uORFs were cloned up stream of the Renilla luciferase gene in a psiCHECK™-2 vector (Promega) using a Gibson Assembly® Cloning Kit (New England Biolabs) ...
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bioRxiv - Microbiology 2019Quote: ... 5 μl of 5× Gotaq buffer (Promega M792A), 1μl of 10μM forward primer ...
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bioRxiv - Biophysics 2021Quote: ... Cells were then incubated overnight at 4 °C with anti-p75NTR (Promega; G323A; 1:300) and anti-TRADD (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: ... Neurons were then incubated overnight at 4° C with anti-p75NTR (Promega; G323A; 1:500) and anti-TRAF6 (Santa Cruz ...
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bioRxiv - Microbiology 2021Quote: ... 4°C) and then resuspended in 100 µl 1 x Passive Lysis Buffer (PLB, Promega). 45 µl of the luciferase assay substrate dissolved in Luciferase Assay Buffer II was plated on a 96-well plate and 10 µl of the cell lysate was added immediately before measurement of Fluc activity using a Tecan reader ...
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bioRxiv - Molecular Biology 2022Quote: ... Compound treatment was initiated 1-4 hours before transfections with FuGENE HD transfection reagent (Promega) following the manual ...
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bioRxiv - Plant Biology 2021Quote: The optimised HaloTag®-7 sequence (298 amino acids) from Promega (https://www.promega.de/) was genetically split on position 155/156 aa into the N-terminal fragment “NHalo” (aa 1-155 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cleaved caspase 7 was measured using a Caspase Glo assay (Promega, Madison, WI) as described by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... 7 and 10 were either cloned with the pGEM®-T kit (Promega) to assay individual sequences and/or sequenced by Illumina (Centre d’expertises et de services Genome Québec) ...
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bioRxiv - Systems Biology 2023Quote: ... 5 x 106 cells were electroporated with 1 μg of NanoLuc pNL3.1 (Promega, N1031) vector and 0.2 μg of control vector ...
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bioRxiv - Evolutionary Biology 2024Quote: ... for 30 minutes then with 5 units/1 L RQ1 RNase-free DNase (Promega) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were rinsed 5 times for 8 min in PBS-tween 0.2% and incubated with secondary antibodies for 2 h at room temperature under agitation (HRP anti-mouse, 1:10000, Promega cat. # W4021). Membranes were further rinsed 5 times for 8 min in PBS-tween 0.2% ...
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bioRxiv - Cancer Biology 2022Quote: ... and the appropriate protein amount (60 μg for baseline expression experiments and 1mg for perturbation experiments) was digested with LysC (1:100, Wako, #121-05063) for 4 h and Trypsin (1:75, Promega, #V5113) overnight ...
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bioRxiv - Genetics 2021Quote: Senescence was measured using 2 assays: 1/ Beta-Glo Assay kit (Promega # E4720), according to the manufacturer’s instructions and utilizing a luciferin-galactoside substrate (6-O-β galactopyranosylluciferin) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transiently transfected with 1 or 2 µg HaloTag-Ubiquitin (N2721, Promega) or the control vector pHTN HaloTag CMV-neo (G7721 ...
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bioRxiv - Cell Biology 2019Quote: ... then alkylation solution was removed and gel pieces were hydrated for 45 min at 4 °C in digestion solution (5 ng/μl trypsin, sequencing grade, Promega, in 25 mM AB). The trypsin digestion proceeded for 2 hours at 37 °C on Thermomixer (750 rpm ...
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bioRxiv - Cell Biology 2019Quote: ... then alkylation solution was removed and gel pieces were hydrated for 45 min at 4°C in digestion solution (5 ng/μl trypsin, sequencing grade, Promega, in 25 mM AB). The trypsin digestion proceeded for 2 hours at 37°C on Thermomixer (750 rpm ...
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bioRxiv - Immunology 2020Quote: ... After a migration time of 4 h at 37°C (5 % CO2) lower compartment were analyzed for cell content by Cell-Titer-Glo® assay (Promega, Madison, WI, USA). Percentages of migrated cells were calculated as described before45 and normalized to the CXCL12-only control.
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bioRxiv - Genomics 2021Quote: ... cells were transfected in a 3:1 ratio of FuGENE® HD Transfection Reagent (Promega cat # E2311) and plasmid DNA (pcDNA3.1-HMGB1P1-3xHA ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 μg of purified RNA was incubated with 3 units of RQ1 DNase (Promega) at 37°C for 30 mins ...
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bioRxiv - Molecular Biology 2021Quote: ... protein digestion was performed by 1 µg of Lys-C (Wako) at 37 °C for 3 h following 1 µg of trypsin (Promega, Madison, WI, USA) at 37 °C for 16 h ...
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bioRxiv - Immunology 2023Quote: ... samples were diluted 1:1 with water and digested for 1.5 hours at 37 °C with 1 µg of LysC and overnight at 37 °C with 1 µg trypsin (Promega). The peptide mixture was acidified with trifluoroacetic acid (Merck ...
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bioRxiv - Neuroscience 2023Quote: ... UDP (at concentrations ranging from 1 nM to 1 mM) was then applied with a final concentration of 5 μM furimazine (NanoLuc Luciferase Assay, Promega). Luminescence was subsequently measured using a Victor X5 multi-label plate reader (PerkinElmer).
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bioRxiv - Biochemistry 2022Quote: ... 3 units/mL RNasin (Promega). Depending on the experiments (see results) ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized via reverse-transcription of 2-5 μg of RNA using M-MLV Reverse Transcriptase (200 U, Promega, Wisconsin, USA), random primers (10 ng/μl ...
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bioRxiv - Biochemistry 2019Quote: ... The samples were then diluted with 150 μL PBS and to this was added 1 mM CaCl2 (2 μL of a 200 mM stock in water) and trypsin (2 μg, Promega, sequencing grade, V5111) and the digestion was allowed to digest overnight at 37 °C with shaking ...
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bioRxiv - Molecular Biology 2020Quote: ... 150 mM NaCl, 1 mM MgCl2, 2 mM EDTA, 1% NP-40, 1 mM DTT, 100 U/mL RNasin [Promega], 1X protease inhibitor) at 7 dpi ...
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bioRxiv - Cell Biology 2021Quote: ... for 4 h at 800 rpm and 42°C or thermolysine (1:50) (Promega, Walldorf, Germany) for 2 h at 800 rpm and 60°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 µL of PBS containing 4 μg/mL Hoechst and 1/10000 CellTox Green Dye (Promega) were were dispensed per well 1 h prior to imaging at Cytation5 image cytometer or Opera Phenix (Perkin Elmer ...
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bioRxiv - Cancer Biology 2023Quote: ... or MEK inhibitor U0126 (1, 10, and 50 µM for 4 h; Promega, Madison, WI, USA). A corresponding volume of dimethyl sulfoxide (Nacalai Tesque ...
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bioRxiv - Molecular Biology 2022Quote: ... terminated by incubation on ice and diluted 1:5 in Glo Lysis Buffer (Promega # E2661). 25 μL of prepared Nano-Glo reagent (Promega # N1120 ...
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bioRxiv - Biochemistry 2023Quote: ... with 25 μL of diluted reactions (diluted 1:5 in Glo Lysis Buffer; Promega # E2661) and incubated at room temperature for 5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... After 7 days of culturing 100 μl of CellTiter-Glo 3D (Promega Cat# G9681) was added to each well and incubated for 5 minutes on a shaker ...
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bioRxiv - Molecular Biology 2019Quote: ... gDNA was isolated after 7 days using Wizard® Genomic DNA Purification Kit (Promega) for preparation of 16S Metagenomic Sequencing Library.
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bioRxiv - Molecular Biology 2021Quote: ... probes listed in Supplementary Table 7 were cloned in pGEM®-T Easy (Promega) for T7 polymerase transcription.
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bioRxiv - Cell Biology 2024Quote: ... biological replicates (n=7) were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described (Hubner et al. ...
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bioRxiv - Microbiology 2022Quote: ... for Calu-3 cells and CellTiter-Glo 3-D Cell Viability Assay Kit (Promega, Germany) for hBAECs according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... A DENV-1 3’UTR specific probe was generated by PCR reaction with GoTaq Polymerase (Promega, Wisconsin, USA) containing DIG DNA-labelling mix (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were transfected with the Piggybac plasmid plus transposase at a 3:1 ratio using Fugene HD (Promega) and selected with G418 (300 µg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... Elution was conducted in 3 beads volume of proteasome buffer containing 1 μL of TEV protease (Promega, PRV6101) for 1 hr at 37°C.
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bioRxiv - Systems Biology 2024Quote: ... followed by a 3:1 dilution with 100mM ammonium bicarbonate and addition of 2µg sequence-grade trypsin (Promega). Samples were digested at room temperature overnight and acidified with formic acid (final concentration 1%) ...
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bioRxiv - Microbiology 2021Quote: ... diluted 1:2 with PBS and read on a GloMax Multi+ Detection System (Promega). CSV files were exported onto a USB flash drive for analysis.
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bioRxiv - Systems Biology 2020Quote: ... The transfections were performed with 1 μg DNA: 2 μl Fugene HD (Promega E2311) ratio ...
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bioRxiv - Microbiology 2022Quote: ... diluted 1:2 with PBS and read on a GloMax Multi+ Detection System (Promega).