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Citations for Promega :
301 - 350 of 670 citations for 6 n Hexylaminopurine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... were transfected with 2 µg of bacmid using Fugene 6 transfection reagent (Promega), as described by the manufacturer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Retrovirus was produced through transfection of HEK293 cells using FuGENE 6 (Promega, E2692). Two days after transfection ...
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bioRxiv - Cancer Biology 2024Quote: HEK-293T cells were transfected using the FuGENE® 6 Transfection Reagent (Promega) following the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2019Quote: ... N-glycans were released from the membrane-bound protein using 2 U PNGase F (Promega, WI, USA) with overnight incubation at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plasmid for β-arrestin-2 fused at the N-terminus to LgBiT was obtained from Promega custom assay services (plasmid CS1603B118) ...
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bioRxiv - Cancer Biology 2023Quote: ... The primer pair (5′- accagacggagtttgagcgcgtcttcTGAGGAGGATCCGGTGGAGCTAGCGGAAGA-3′ and 5′-ctccaccgagtcgtactgcttcgccatACCAGAATTCCCACCGCTCGAGCCA-3′) and pBit3.1-N (Cat. No. N2361, Promega) were used to clone the vector-containing fragment ...
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bioRxiv - Neuroscience 2020Quote: Transfected neuronal cultures with the NF-κB reporter Firefly Luciferase plasmid (Cat. N° E1980, Promega, Madison, WI, USA), were stimulated with NMDA/glycine for 60 minutes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... NanoBiT plasmids pBiT1.1-N_[TK/SmBiT] and pBiT2.1-N_[TK/LgBiT] were purchased from Promega (cat n° N2014).
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bioRxiv - Biochemistry 2019Quote: ... The N-terminal 312 aa Halo tag sequence was PCR amplified from His6HaloTag® T7 Vector pH6HTN (Promega) as a 5’ SpeI ...
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bioRxiv - Molecular Biology 2020Quote: ... 300 ng CBS-VN (venus N-terminal) and 200 ng pmCherry-C1 plasmids) by using Fugene HD (Promega). At 24 h post-transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:50 ...
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bioRxiv - Biochemistry 2022Quote: ... Rat Gβ1 with an N-terminal MHHHHHHSSGLVPRGSHMASHHHHHHHHHH-tag (His16) was fused with the SmBiT subunit (peptide 86, Promega)20 via a 15 amino acid (GSSGGGGSGGGGSSG ...
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bioRxiv - Cell Biology 2022Quote: ... β-arrestin 2 fused at the N-terminus to LgBiT (LgBiT-β-arrestin 2; Promega, plasmid no. CS1603B118) was chosen as it has previously been used successfully with other class B GPCRs ...
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bioRxiv - Biophysics 2023Quote: ... equal volumes of HiBiT-N VLPs and hACE2-LgBiT EVs were mixed with nanoluc substrate (cat#N2420, Promega) and trypsin (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... pucks (n=3) for each cell density condition underwent the BacTiter-Glo™ Microbial Cell Viability Assay (Promega), as described in the manufacturer’s instruction manual ...
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bioRxiv - Cell Biology 2024Quote: ... This cDNA was assembled into a vector encoding N-terminal fusion to the HaloTag (pHTN_HaloTag_CMV-neo, Promega G7721) using restriction cloning ...
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bioRxiv - Cell Biology 2020Quote: ... Transfection was performed in the 6-well culture plate using FuGene-HD (Promega, E2311) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Retrovirus was produced in Phoenix Amphotropic cells by transfection with FUGENE 6 (Promega E2692) of pLPC-TRF2 or pLPC-Empty ...
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bioRxiv - Cell Biology 2021Quote: ... and a pcDNA5/FRT plasmid of interest using the Fugene 6 transfection kit (Promega) or Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... Plates were collected at day 6 and measured by CellTiter-Glo (Promega #PR-G7573) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: HEK 293T cells grown on 10 cm dish were transfected using FuGENE 6 (Promega) with the transfer vector pxPAX2 (Addgene #12260 ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were transiently transfected with 0.4 µg DNA using FuGENE 6 transfection reagent (Promega) in a 1:3 µg DNA:µL FuGENE ratio 48 h before electrophysiology measurements were performed.
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bioRxiv - Immunology 2020Quote: ... China) or the D614G variant (constructed by site-directed mutagenesis) using FuGENE 6 (Promega). The next day ...
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bioRxiv - Developmental Biology 2020Quote: ... For the immunolabeling experiment HeLa cells were transfected with plasmids using FuGENE 6 (Promega). For streptavidin pull-down assays from HEK293T cells ...
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bioRxiv - Genetics 2021Quote: ... Plasmid transfection of U2OS cells was carried out using FuGENE 6 Transfection Reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Transfection with plasmid was performed following standard protocols for Fu gene 6 (Promega, #E2691). Cells were selected for stable expression using Geneticin (G418 sulfate ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of both plasmids was mixed with 6 μl FuGENE reagent (Promega E269A) in 200 μl serum free DMEM and incubated for 30 minutes before adding the transfection mix to the cells ...
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bioRxiv - Physiology 2021Quote: ... allowing measurement of 2-deoxyglucose-6-phosphate using the Glucose Uptake-Glo assay (Promega) and a FLUOstar Omega luminescence plate reader.
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bioRxiv - Biophysics 2021Quote: ... U2OS cells transfection with engineered lentiCRISPRv2 were done using FuGENE 6 transfection reagent (Promega). Positive clones were selected using puromycin and subsequently isolated.
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bioRxiv - Cell Biology 2022Quote: ... Bacmids were transfected to Sf9 insect cells by lipofection (FuGENE 6 Transfection Reagent, Promega), to generate baculovirus ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid transfection of U2OS cells was carried out using FuGENE 6 Transfection Reagent (Promega) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of both plasmids was mixed with 6 μl FuGENE reagent (Promega E269A) in 200 μl serum free DMEM and incubated for 30 min before adding the transfection mix to the cells ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected with MX2-mScarlet vector using FuGENE 6 transfection reagent (#E2691, Promega) following the manufacturers protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Plates were collected at day 6 and measured by CellTiter-Glo (Promega #PR-G7573). Cell viability values were analyzed following blank cell deductions and normalization to vehicle readings ...
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bioRxiv - Biochemistry 2024Quote: ... 24 hours after transient transfection with mouse or human ENPP3 via Fugene 6 (Promega), the media was gently removed and replaced with serum-free DMEM supplemented with 1% insulin-transferrin-selenium-sodium pyruvate (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with TPMT-FLAG or TPMT A80P-FLAG with FuGENE 6 (Promega). 24 h post transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng/μl of mRNA and 6 ng/μl QuantiLum Recombinant Luciferase protein (Promega) were coinjected ...
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bioRxiv - Microbiology 2022Quote: ... DNA from the saliva samples (n=67, one missing) was extracted using the ReliaPrep Blood gDNA Miniprep (Promega, USA). For microbiome profiling ...
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bioRxiv - Systems Biology 2019Quote: ... with N-and C-terminus tagging configurations (NB MCS1, NB MCS2, NB MCS3, NB MCS4) were obtained from Promega, each containing an HSV-TK promoter for protein expression in mammalian cells and an ampicillin resistance marker for bacterial selection of transformed cells ...
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bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
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bioRxiv - Microbiology 2021Quote: ... The SK-N-SH cell surface proteins with attached TBEV virions were digested off with 1.25 μg trypsin (Promega) and the supernatant collected ...
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bioRxiv - Cancer Biology 2022Quote: ... RT) and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:100 ...
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bioRxiv - Immunology 2020Quote: ... pH 8.0 using Vivaspin columns (3 kDa) and digested separately O/N using trypsin or chymotrypsin (Mass Spectrometry Grade, Promega) at a ratio of 1:30 (w/w) ...
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bioRxiv - Molecular Biology 2022Quote: ... A second digestion was performed o/n at 37°C while shaking at 1,400 rpm using 500 ng of sequencing grade modified trypsin (Promega). Trypsin digested peptides were separated from Ni-NTA beads by filtering through a 0.45 µm filter Ultrafree-MC-HV spin column (Merck-Millipore).
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were released from the intact proteins using 0.5 U/ µL N-glycosidase F (PNGase F, Elizabethkingia miricola, Promega), overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... RT) and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:100, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:100 ...
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bioRxiv - Biophysics 2022Quote: ... pHalo-SMARCA4 expresses human SMARCA4 with HaloTag fused to the N-terminus under a CMVd1 promoter (Promega ORF FHC12075). pHalo-MED26 expresses human MED26 fused with a HaloTag at the N-terminus and was a kind gift from Joan Conaway’s lab ...
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bioRxiv - Molecular Biology 2023Quote: ... A second digestion was performed o/n at 37°C while shaking at 1,400 rpm using 500 ng of sequencing grade modified trypsin (Promega). Trypsin digested peptides were separated from Ni-NTA beads by filtering through a 0.45 μm filter Ultrafree-MC-HV spin column (Merck-Millipore).
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bioRxiv - Molecular Biology 2023Quote: ... Full-length GID4 N or C-terminal NanoLuc-fusion was transfected into HEK293T cells using FuGENE HD (Promega, E2312) according to manufacturer’s instructions and proteins were allowed to express for 20 hours ...
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bioRxiv - Immunology 2023Quote: ... and digested for 4 hrs with 2 mg/ml LysC (Wako Chemicals GmbH) in a ratio of 1:50 and O/N with 0.1 µg/µl trypsin (Promega) in a ratio of 1:50 ...