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Citations for Promega :
601 - 650 of 684 citations for 6 decenoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cells grown to ∼70% confluence in 6-well plates and co-transfected with a gRNA to TBC1D23 (12) and pmaxGFP using Fugene6 (Promega) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... mammalian expression vectors containing the fusion gene under the control of a weak L30 promoter and containing a Neomycin resistance marker were transfected into U2OS cells at 70% confluence using FuGENE 6 (Cat. No. E2691, Promega). Transfected cells were selected with G418 (Cat ...
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bioRxiv - Cell Biology 2024Quote: ... and RSPO1-CM were produced as described (Fenderico et al., 2019; Tauriello et al., 2010) Transfections were performed using either FuGENE 6 (Promega) according to manufacturer’s protocol for β-catenin-mediated reporter assays and microscopy or polyethylenimine (PEI ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... plated in 6-well dishes were centrifuged at 1000xg for 10 minutes and suspended in 5x Passive Lysis buffer (Promega). Each data point represents an individual well ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assayed starting at Day 1 for 6 days according to the CellTiter-Glo 3D kit (Promega G9683). For the antiandrogen response assay ...
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bioRxiv - Molecular Biology 2024Quote: About 5 μg empty pcDNA3 vector or ZNF410 full-length and ZNF410-ZF plasmids was transiently transfected with FuGENE 6 (Promega) into 10 cm plates of COS-7 cells ...
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bioRxiv - Biochemistry 2024Quote: ... at a 1:75 enzyme to protein ratio for 6 h at 30 °C and then by trypsin (V5111, Promega) (1:50 enzyme to protein ratio ...
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bioRxiv - Neuroscience 2023Quote: Approximately 5x104 HEK293 cells were seeded on poly-L-lysine coated glass coverslips and transfected with expression constructs for SORL1-WT or SORL1-R953C using Fugene 6 Transfection Reagent kit (Promega). 24h post-transfection ...
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bioRxiv - Microbiology 2019Quote: ... Viral RNA was isolated from homogenized tissues using the Maxwell 48 RSC Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI) on a Maxwell 48 RSC instrument ...
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bioRxiv - Molecular Biology 2021Quote: ... In vitro translation reactions were carried out with 5 ul of either supplemented rabbit reticulocyte lysate or oocyte extract in total volume of 20 µl containing 10 µM amino acid mix (Promega, L4461), 0.5 units SUPERase•In ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from lung tissue using the Maxwell automated instrument and the Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega). The concentration of RNA and quality were measured with HS Total RNA 15nt.methods (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... for 4 hours at 37°C and boosted with an extra 2µl Trypsin/LysC (Promega V5073: 20ug + 80uL 50mM acetic acid) overnight at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... supernatant was harvested from Calu-3 cells and inactivated by addition of lysis buffer (Maxwell 16 viral total nucleic acid purification kit, Promega #AS1150) complemented with proteinase K ...
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bioRxiv - Microbiology 2021Quote: RNA from lysed and heat-inactivated samples was purified according to the instructions of the manufacturer using Maxwell 16 viral total nucleic acid purification kit (Promega, #AS1150) or Maxwell 16 LEV simplyRNA cell kit (Promega ...
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bioRxiv - Microbiology 2022Quote: Total RNA from each lung homogenate and nasal wash was isolated using the Maxwell RSC Maxwell RSC Viral Total Nucleic Acid Purification Kit (Promega Corporation), following the manufacturer’s suggested protocol ...
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bioRxiv - Microbiology 2023Quote: ... the ladder region was cut off and stained for 30 min in the running buffer containing 10000x diluted Diamond™ Nucleic acid dye (Promega). The transfer of the RNA from the agarose gel to the nylon membrane (Amersham Hyband™-N+ ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic Acid Purification with Proteinase K digestion was performed using Maxwell ® RSC Total Viral Nucleic Acid Purification Kit according to the manufacturer’s protocols (Promega, USA). For preparation of bacterial genomic DNA ...
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bioRxiv - Immunology 2021Quote: ... vRNA was isolated from plasma samples using the Maxwell Viral Total Nucleic Acid Purification kit on the Maxwell 48RSC instrument (Promega, Madison WI). vRNA was then quantified using a highly sensitive qRT-PCR assay based on the one developed by Cline et al.99 ...
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bioRxiv - Microbiology 2021Quote: ... The reaction mixture was prepared into 96-well plates as follows: 1 μl of 1 mM amino acid mixture minus methionine (Promega, Cat: L9961), 35 μl of rabbit reticulocyte lysate ...
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bioRxiv - Pathology 2022Quote: ... or 300 uL urine+DMSO using the Maxwell RSC Viral Total Nucleic Acid Purification Kit on the Maxwell 48 RSC instrument (Promega, Madison, WI).
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from these bacterial suspensions using a Maxwell® 16 automated nucleic acid purification system and the Maxwell® tissue DNA purification kit following the manufacturer’s instructions (Promega, UK). Purified DNA extracts were treated with RNase (New England BioLabs ...
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bioRxiv - Microbiology 2019Quote: Viral RNA was isolated from plasma samples using the Maxwell 16 Viral Total Nucleic Acid Purification kit on the Maxwell 16MDx instrument (Promega, Madison WI). Viral RNA was then quantified using a highly sensitive qRT-PCR assay based on a previously published protocol115.
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bioRxiv - Molecular Biology 2019Quote: RNA was extracted using Maxwell® 16 LEV RNA Cartridge in automated Maxwell® 16 nucleic acid extraction instrument following manufacturer’s protocol (Promega, USA). The extracted RNA was stored at −80°C until further use ...
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bioRxiv - Molecular Biology 2020Quote: ... the reaction mix in a final volume of 83 µl has been prepared by adding 21 µl of the Amino Acid Mixture (for a final concentration of 0.13 mM) and 80 units of RNasin (Promega Ref. number N2511) to 60 µl of RRL ...
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bioRxiv - Evolutionary Biology 2021Quote: Viral RNA (vRNA) was extracted from 100 μl of VTM using the Viral Total Nucleic Acid Purification kit (Promega, Madison, WI, USA) on a Maxwell RSC 48 instrument and eluted in 50 μL of nuclease-free H2O.
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bioRxiv - Microbiology 2019Quote: ... RNA was extracted using the Maxwell 16 LEV simplyRNA tissue kit in combination with the Maxwell nucleic acid extraction robot (Promega, WI, USA), according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: Total nucleic acid extraction from swab and tissue samples was performed using ReliaPrep™ Viral TNA Miniprep System (Promega, Madison, WI, USA). Swab samples were simply inverted in the tube and briefly centrifuged prior to extraction ...
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bioRxiv - Microbiology 2020Quote: ... Transfection of HEK293T cells was performed at 80% confluence using 25ng of each plasmid construction mixed with 150nL of Fugene 6 (E2691, Promega, France) reagent per well ...
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bioRxiv - Cell Biology 2020Quote: ... gel regions were diced and proteins were subjected to in-gel digestion with 6 ng/μL trypsin (Promega, Madison, WI, USA) in 50 mM ammonium bicarbonate at 37 °C for 10-12 hours ...
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bioRxiv - Cell Biology 2020Quote: ... 250 ng of each of the donor and Cas9-sgRNA vectors were transfected to RPE-1 cells cultured in 6 wells with 1.5 μl of FuGENE® HD (Promega, E2311) overnight ...
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bioRxiv - Genomics 2019Quote: ... pSMGV Dam-V5-Lap2β (Dam-Lap2β) and pSMGV Dam-V5-EMD (Dam-EMD) were transfected using Fugene 6 transfection reagent (Promega, E2691) into the Platinum-E packaging line (Cell Biolabs ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were digested overnight at RT with a 1:50 ratio of sequencing grade modified trypsin (0.4 μg for whole proteome samples, 6 μg for phospho samples, V511A, Promega, Fitchburg WI) in 130 μL TEAB (0.05M Triethylammoniumbicarbonate in water) ...
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bioRxiv - Cancer Biology 2022Quote: ... Media was refreshed on day 6 and plates collected on day 12 with cell viability quantified by CellTiter-Glo 3D (Promega #G9681). Dose response assay analysis was performed as described above.
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bioRxiv - Molecular Biology 2021Quote: ... and cells with approximately 70-90% confluency in each plate were transfected with required plasmid(s) for each assay using Fugene 6 transfection reagent (Promega, USA) or 1 mg/ml PEI (polyethyleneimine).
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bioRxiv - Microbiology 2020Quote: Plasmids encoding each vector were transfected into HEK293 cells seeded in 10 cm plates using Fugene 6™ (Promega, Madison, WI) or polyethyleneimine ...
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bioRxiv - Cell Biology 2019Quote: ... a puromycin resistance marker and specific gRNA guides against VPS35, SNX5 or SNX6 (Kvainickas et al., 2017; Simonetti et al., 2017) using FuGENE® 6 (Promega). The day after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
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bioRxiv - Cancer Biology 2020Quote: ... used for infection of p53-null NSCs were obtained from previously transfected DF1 chicken fibroblasts (ATCC #CRL-12203) using FuGENE 6 Transfection reagent (Promega #E2691), according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... the cells were transiently transfected with pUC57 constructs harboring 1.3-mer HBV genomes (genotypes A2, B2, C2, and D3) using FuGene 6 transfection reagent according to the manufacturer’s instructions (Promega, WI, USA). The generation of plasmids has been previously described and this transient expression system relies on the endogenous promoters of HBV for transcription (6) ...
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bioRxiv - Molecular Biology 2019Quote: ... Transient transfections were performed in 24-well plates using 500ng of dCas9 expression vector and 250ng of equimolar pooled or individual sgRNA expression vectors mixed with FuGENE® 6 (Promega) following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: XAB2-GFP and RNAP2-GFP stably expressing cell lines were produced by transfecting XAB2-GFP or RNAP2-GFP using FuGENE® 6 Transfection Reagent (Promega) according to the manufacturer’ protocol ...
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bioRxiv - Immunology 2021Quote: Pseudoviruses expressing complete SARS-CoV2 spike genes were prepared by transient transfection of HEK293T cells with three plasmids: SARS-CoV2 MLV-gag/pol and MLV-CMV-luciferase plasmids using Fugene 6 (Promega Inc.) as described earlier (Rogers et al. ...
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bioRxiv - Microbiology 2022Quote: ... The effector cells and serum were incubated with the cells for 6 hours after which 75ul Bio-Glo reagent (Promega; G7940) was added to each well ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected with 12 µg of plasmid expressing the spike from various variants using Fugene-6 (Promega; catalog number: E2691) according to the manufacturer’s instructions and incubated at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... transfection was carried out by adding 2 μg of freshly prepared bacmid DNA and 6 μL of FuGene HD transfection reagent (Promega, E2311), following the instructions provided by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... or pCEP4-OCT4-T234A-S235A (addgene, 40630) or pCEP4-OCT4-T234E-S235E (addgene, 40631) for 48 h using FuGENE 6 (Promega, E2691) according to the manufacturer’s protocol.
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bioRxiv - Physiology 2024Quote: ... a targeting vector was constructed by subcloning a 9.1 kb region from an Akr1b7 positively identified C57BL/6 fosmid clone (WI1-1577M19) into the 2.45 kb-iTL cloning vector derived from the pSP72 vector (Promega; Madison, WI). The region was designed so the long homology arm (LA ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected the following day using 0.6–1 µg of plasmid and Viafect reagent (Promega E4981, 6 µl/µg plasmid). The medium was exchanged the following day to regular culture medium ...
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bioRxiv - Microbiology 2024Quote: ... and simultaneously transfected with 0.4 μg of respective EGFP-Rab plasmids using FuGENE 6 transfection reagent (Cat. E269A, Promega, Madison, WI) per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and gene expression was determined by quantitative PCR (QuantStudio 6 Pro Real-Time PCR System) with GoTag qPCR System (Promega, A6002). Actb and Rplp0 were used as housekeeping genes ...