Labshake search
Citations for Promega :
301 - 350 of 1229 citations for 6 Propoxypyridazin 3 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2019Quote: ... Transfection of plasmids into cells were performed using FuGENE 6 (Promega) according to the manufacturer’s protocol and the cells were harvested 24 hours after transfection ...
-
bioRxiv - Cell Biology 2019Quote: HeLa cells were transfected with EB3-GFP using FuGENE 6 (Promega) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... All transfections were performed with FuGENE 6 transfection agent (Promega E2691) in Opti- MEM Reduced Serum Medium (Gibco 31985070 ...
-
bioRxiv - Biophysics 2020Quote: ... Transfection was performed using Fugene 6 transfection reagent (Promega, Madison, WI). When WT and mutant myc-KCNQ1 were co-transfected ...
-
bioRxiv - Cancer Biology 2021Quote: ... expression plasmids were delivered with Lipofectamine 3000 or FuGene 6 (Promega) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... a total 3μg of plasmid DNA and 6μL FuGENE 6 (Promega) were combined in 300μL Opti-MEM (Gibco) ...
-
bioRxiv - Systems Biology 2021Quote: ... HEK293 cells were co-transfected using Fugene 6 transfection reagent (Promega) with MAC-tag (600ng ...
-
bioRxiv - Microbiology 2020Quote: ... cells were treated with 6 μM EnduRen (Promega, Madison, WI, USA), a substrate for Renilla luciferase ...
-
bioRxiv - Biophysics 2021Quote: pGFP was transfected using FuGENE®6 Transfection Reagent (E2691,Promega) with OptiMEM (31985088 ...
-
bioRxiv - Immunology 2022Quote: ... Phoenix cells were transfected using FuGENE 6 Transfection Reagent (Promega #E2691), and retroviral supernatant was collected and used immediately for transfection of MC38s on days 2 and 3 following transfection ...
-
bioRxiv - Biochemistry 2022Quote: ... and 0.4 ng plasmid encoding Renilla luciferase using FuGENE 6 (Promega). Luciferase activity was measured 48 h post-transfection using the Dual Luciferase assay kit (Promega ...
-
bioRxiv - Cancer Biology 2022Quote: Transfections were carried out using FuGENE 6 transfection reagent (Promega #E2691) per the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... and target (400 ng) plasmids using 6 μL FuGene HD (Promega). 72 hrs later ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 2.7 μl FuGENE 6 Transfection Reagent (E2691, Promega, Madison, WI) in 1.5 ml volume ...
-
bioRxiv - Physiology 2021Quote: ... and 5 μg lentiviral construct with 30 μL Fugene 6 (Promega) the following day ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were transiently transfected using Fugene 6 (Promega, Madison, WI, #E2691) as per the manufacturer’s instructions overnight in a 25-cm2 cell culture flask (Genessee Scientific Corporation ...
-
bioRxiv - Immunology 2020Quote: ... Plasmids were mixed with Fugene 6 transfection reagent (Promega, Madison, WI) at a dilution of 1:12 in a total volume of 600 μl ...
-
bioRxiv - Cell Biology 2021Quote: ... The plasmids were transfected into HeLa cells using Fugene 6 (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... were transfected into C2C12 cells using a Fugene 6 reagent (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were transiently transfected with the help of Fugene 6 (Promega) using a ratio of 3:1 of reagent to DNA ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 2.25 μg psPax2) and 18 μL of FuGENE 6 (Promega). The culture medium was changed 6-24 h later ...
-
bioRxiv - Microbiology 2023Quote: ... and 0.25 μg VSV-G envelope plasmid using Fugene 6 (Promega) transfection reagent ...
-
bioRxiv - Cell Biology 2023Quote: Cells expressing one plasmid were transfected with FuGENE 6 (Promega #E2691) at a FuGENE:DNA (μL:μg ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with the indicated plasmids using FuGENE 6 (Promega) or Lipofectamine 3000 (Invitrogen ...
-
bioRxiv - Cell Biology 2023Quote: ... then digested by treatment with 6 µl of Trypsin Gold (Promega). Peptides were extracted and concentrated for MS analysis.
-
bioRxiv - Molecular Biology 2024Quote: ... Plasmid transfections were carried out using FuGENE 6 Transfection Reagent (Promega) according to the manufactureŕs protocol.
-
bioRxiv - Microbiology 2023Quote: ... and DNA transfection was performed using Fugene 6 reagent (E2691, Promega). 1 μg of MPXV DNA was mixed with 3 μl of Fugene 6 reagentin 100 μl Opti-MEM medium for 15 minutes at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... Plasmid transfection was carried out using FuGENE 6 Transfection Reagent (Promega) according to the manufacturer’s protocol.
-
bioRxiv - Cell Biology 2024Quote: ... then transfected with 1.5 µg plasmid DNA using FuGENE 6 (Promega) as follows ...
-
bioRxiv - Microbiology 2019Quote: ... 500 nM of each primer (5’-TCCTGCTCAACTTCCTGTCGAG-3’ and 5’-CACAGGTCAAACCTCCTAGGAATG-3’) and 0.1 µL hot-start Taq DNA polymerase (Promega) in 20 mM Tris−HCl pH 8.3 ...
-
bioRxiv - Cancer Biology 2022Quote: ... caspase-3 and caspase-7 activities were measured using a Caspase Glo 3/7 Assay (G8090; Promega, WI, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: The activity of caspase-3/7 was measured by Caspase-Glo-3/7 assay kit according to the manufacturer’s instructions (Promega). Cell death was assessed by an Annexin-V FITC binding assay (Miltenyi ...
-
bioRxiv - Biochemistry 2021Quote: Caspase-3/7 activity was quantified by fluorometric assay using ApoONE Homogeneous Caspase-3/7 Assay Kit (Promega, US). AU565 cells ...
-
bioRxiv - Cancer Biology 2020Quote: ... was determined at day 3 and 4 post-transfection using the Caspase-Glo 3/7 Assay (Promega, Mannheim, Germany). The emerging fluorescence was detected (485Ex/527Em ...
-
bioRxiv - Microbiology 2023Quote: ... 20 μL of MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) inner salt (Promega) was added to each well and the plate was further incubated for 1 h at 37 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... 20 μL of [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] (MTS) (Promega, #G3582) were added and incubated at 37°C for 3 hours ...
-
bioRxiv - Microbiology 2021Quote: ... Standard RNA was synthesized from a partial region of the GPC gene using the forward (5’-TAATACGACTCACTATAGGGCCAACCTTTTTGCAGGAGGC-3’) and reverse (5’-AGCTTCTTCTGTGCAGGATCTTCCTGCAAGCGCTAGGAAT-3’) primers and the T7 RNA polymerase (Promega), as previously described (Pemba et al. ...
-
bioRxiv - Microbiology 2022Quote: ... The region of recombination was amplified using primers PV3-F2 (5′-CTCCAAAGTCCGCATTTACA-3′) and PV1-R2 (5′-ATCAGGTTGGTTGCTACA-3′) and Taq polymerase (Promega) with an initial denaturing at 95°C for 2 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... The following day activity of caspases 3 and 7 was measured using the Caspase-Glo® 3/7 Assay (Promega), according to the manufacturer’s instruction.
-
bioRxiv - Cell Biology 2019Quote: ... The full-length coding sequence of murine Tmem98 was amplified from a mix of murine embryonic and murine keratinocyte cDNA using forward 5’-AAAAAGCTTGCCATGGAGACTGTGGTGATCGTC-3’ and reverse 5’-TTTTGAATTCTTAAATGGCCGACTGTTCCTGCAGGAAGC-3’ primers and cloned into pSP72 (Promega) as a HindIII/EcoRI fragment ...
-
bioRxiv - Microbiology 2021Quote: ... Apoptosis was quantified by measuring caspase 3/7 activation of a luminescent signal using Caspase-Glo 3/7 assay (Promega).
-
bioRxiv - Cancer Biology 2021Quote: Caspase 3/7 and 9 activities were assessed using a fluorescence-based Apo-ONE homogenous caspase 3/7 assay kit (Promega) and luminescence-based caspase-glo 9 assay system (Promega) ...
-
bioRxiv - Cancer Biology 2021Quote: ... The resulting 3’UTR-Wild and 3’UTR-Mutant genes were inserted into the XbaI restriction site of pGL3-control vector (Promega) expressing firefly luciferase ...
-
bioRxiv - Cancer Biology 2020Quote: ... grown for 48 h and then caspase 3/7 enzymatic activity determined using Apo-One Homogenous caspase 3/7 activity assay kit (Promega) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... 5 pmol of each primer (C1105 5’-GGTTCATCGACATCTCCGCG-3’ and C1106 5’-AGGTCGCTGCGCATGCCAATC-3’) and 1.25 units of GoTaq® DNA polymerase (Promega). The cycling conditions were as follows ...
-
bioRxiv - Cancer Biology 2020Quote: ... Caspase3/7 activity was measured 3 days after the treatment with a commercial available kit (Caspase-Glo 3/7 Assay, Promega).
-
bioRxiv - Cell Biology 2021Quote: ... a ~600 bp mouse ATF6β cDNA fragment was PCR-amplified using 5’-AACAGGAAGGTTGTCTGCATCAT-3’ and 5’-GTATCCTCCCTCCGGTCAAT-3’ primers and inserted into the pGEM-T vector (Promega). The plasmid was linearized using EcoRV and ApaI to synthesize the antisense and sense probe ...
-
bioRxiv - Cell Biology 2022Quote: ... Caspase 3/7 activity was assayed according to the manufacturer’s protocol for ApoOne® Homogenous Caspase 3/7 Assay (Promega). Fluorescence was measured with Glomax Multi plate reader (Promega).
-
bioRxiv - Cancer Biology 2022Quote: ... Treatments were removed 48 or 72 h later and enzymatic activities of caspase-3 and -7 were measured using Caspase-Glo 3/7 Assay (Promega) and read by a microplate reader (SpectraMax M5).
-
bioRxiv - Cancer Biology 2022Quote: Caspase 3/7 activity was measured using a luminescence Caspase Glo 3/7 assay kit (G8090; Promega, Madison, WI, USA) according to the manufacturer’s instructions ...