Labshake search
Citations for Promega :
201 - 250 of 3123 citations for 6 Phenyl hexa 3 5 dien 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... and 20 µL of 1:1000 (diluted in phenyl red-free DMEM with 4% FBS) HaloTag™ NanoBRET™ 618 Ligand (Promega G9801) was added to each well ...
-
bioRxiv - Cell Biology 2019Quote: Glucose uptake was measured based on the detection of 2-deoxyglucose-6-phosphate uptake by a commercially available luminescence-based kit (Glucose Uptake-GloTM Assay, Promega) on a SpectraMax M3 (Molecular Devices) ...
-
bioRxiv - Cell Biology 2019Quote: ... HEK293T cells were grown in two 175 cm2 flasks to ∼50% confluence and transfected with 25 μg plasmid and 75 μl FuGENE 6 in 2 ml Opti-MEM according to the manufacturers instructions (Promega). One day after transfection ...
-
bioRxiv - Biochemistry 2021Quote: ... HEK293T cells and C3H10T1/2 cells were transfected in 6-well plates with various constructs of pmirGlo vector (Promega®). Transfection was performed using Turbofect transfection reagent (Thermo Fischer scientific® ...
-
bioRxiv - Biochemistry 2021Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV-2-spike (kindly provided by Dr. Peihui Wang, Shandong University, China) using FuGENE 6 (Promega). Cells were cultured overnight at 37 °C with 5% CO2 ...
-
bioRxiv - Cell Biology 2019Quote: Desired protein amounts were aliquoted and chloroform methanol precipitated, followed by digestion with LysC (overnight at room temperature, vortex speed 2; Wako) and trypsin (6 hours, 37° Promega) digestion ...
-
bioRxiv - Immunology 2021Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV-2-spike (kindly provided by Dr. Peihui Wang, Shandong University, China) using FuGENE 6 (Promega). Cells were cultured overnight at 37°C with 5% CO2 ...
-
bioRxiv - Biochemistry 2023Quote: ... 1 × 106 Sf9 cells in 2 mL of media in a 6-well dish were transfected with up to 2 µg of fresh bacmid DNA using FuGene HD transfection reagent (Promega) at a ratio of 3:1 (Fugene reagent:DNA ...
-
bioRxiv - Immunology 2023Quote: ... and the corresponding SARS-CoV-2 spike construct were co-transfected in HEK293-T cells using FuGENE 6 Transfection Reagent (Promega) in Dulbecco’s Modified Eagle Medium (DMEM ...
-
bioRxiv - Microbiology 2020Quote: ... HeLa cells were transfected 6 hours before imaging with Fugene 6 (Promega) and 1000ng of DNA ...
-
bioRxiv - Cell Biology 2020Quote: ... using Fugene 6 (Promega). Supernatant was harvested from the transfected cells for the next three days and then centrifuged ...
-
bioRxiv - Cell Biology 2020Quote: ... using Fugene 6 (Promega), with a linearized TCIS construct described previously6 ...
-
bioRxiv - Genetics 2021Quote: ... using FuGENE 6 (Promega) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... using FuGENE 6 (Promega). Sixteen hours later ...
-
bioRxiv - Cancer Biology 2020Quote: Transfection: Fugene 6 (Promega); TransIT-2020 (Mirus Bio) ...
-
bioRxiv - Biophysics 2020Quote: ... FuGENE 6 (Promega, E2691) was used for transient transfection according to the manufacturer’s instructions.
-
bioRxiv - Genetics 2019Quote: ... using FuGENE 6 (Promega) on day 0 ...
-
bioRxiv - Developmental Biology 2022Quote: ... or Fugene 6 (Promega) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2020Quote: ... using FuGENE 6 (Promega). Cells were cultured overnight at 37°C with 5% CO2 ...
-
bioRxiv - Cell Biology 2021Quote: ... using Fugene 6 (Promega).
-
bioRxiv - Microbiology 2020Quote: ... using FuGENE 6 (Promega). Sixteen hours later ...
-
bioRxiv - Cell Biology 2022Quote: ... or FuGENE 6 (Promega) following the manufacturer’s protocol with modifications (see below).
-
bioRxiv - Microbiology 2022Quote: ... or Fugene 6 (Promega) and supernatants harvested 3 days later ...
-
bioRxiv - Cell Biology 2023Quote: ... utilizing Fugene 6 (Promega). Culture supernatants were collected at 48h and 72h post-transfection ...
-
bioRxiv - Cell Biology 2020Quote: ... 100 μL One-Glo-EX (Promega, E6120) was added into the cells for incubation in the dark for 10 min prior to reading on an Enspire 2300 multilable reader (Perkin Elmer ...
-
bioRxiv - Microbiology 2021Quote: ... and 30U of RNase One (Promega, USA) in 1x DNase buffer (Ambion ...
-
bioRxiv - Microbiology 2021Quote: ... 30 μl of ONE-Glo reagent (Promega) was added according to manufacturer’s protocol and luminescence was quantified using the BioTek Synergy 2 Plate reader ...
-
bioRxiv - Plant Biology 2020Quote: ... 1 unit of RNase ONE™ (Promega) or 0.01 unit of RNase V1 (Thermo Fischer Scientific ...
-
bioRxiv - Plant Biology 2021Quote: ... A One-step RT-qPCR kit (Promega) was used for converting the RNA into cDNA before amplifying target transcripts on a CFX Connect Real-Time PCR Detection System (Biorad) ...
-
bioRxiv - Microbiology 2021Quote: ... or the QuantiFluor ONE dsDNA System (Promega). Indexed amplicons were pooled at equimolar concentration ...
-
bioRxiv - Neuroscience 2021Quote: ... The CytoTox-ONE Membrane Integrity Assay (Promega) was performed according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... One microgram of sequencing grade trypsin (Promega) dissolved in water was added to the protein solution and the specific proteolytic digestion proceeded for 16 h at 37 °C in a thermomixer (Thermo Fisher Scientific) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... 30 μL ONE-Glo reagent (Promega, E6120) was added to each well ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 40 units of RNase ONE (Promega) for 1.5 hr at 4°C with end-over-end rotation ...
-
bioRxiv - Neuroscience 2019Quote: ... together with one of the pGL4 (Promega) luciferase vectors generated with enhancer sequences as described above ...
-
bioRxiv - Microbiology 2020Quote: ... One μg of sequencing grade trypsin (Promega) was then added per sample and digestion was carried out overnight at 37°C ...
-
bioRxiv - Cell Biology 2022Quote: ... and 100 µl One-Glo-EX (Promega) was added to the cells in equivalent MEM with no serum volume and incubated in the dark for 5 min ...
-
bioRxiv - Physiology 2022Quote: CellTiter 96 AQueous One Solution Reagent (Promega) was used to assess the effect of EVs on cell proliferation rate in in vitro autotransplantation approach (n=4) ...
-
bioRxiv - Genetics 2023Quote: CellTiter 96 AQueous One Solution (Promega G3580) was added to fresh BM-MSC complete growth media ...
-
bioRxiv - Cancer Biology 2023Quote: ... CellTitre 96 AQueous One Solution MTS (Promega) was added to cells plated at low density according to manufacturer’s instructions and incubated in darkness in a humidified incubator for 3h at 37°C ...
-
bioRxiv - Microbiology 2023Quote: CellTiter 96 AQueous One Solution (G3580, Promega) was used ...
-
bioRxiv - Microbiology 2023Quote: ... One μg of sequencing grade trypsin (Promega) was then added per sample and digestion was carried out overnight at 37°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... CellTiter 96 Aqueous One Solution Assay (Promega) was added (20 μL per well) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Plasmid DNA (6 μg) was delivered into U2OS cells using FuGENE 6 (Promega) and 2.5 μg of plasmid DNA was delivered into HeLa cells using Lipofectamine 2000 (Invitrogen ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were incubated at 37°C/5% CO2 for 3 days before performing CellTiter-Glo (CTG) assays as per the manufacturer’s instruction (Promega). Luminescence was read using a Molecular Devices Spectramax L plate reader ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were incubated at 37°C and 5% CO2 for 3days before CellTiter-Glo (CTG) assays were performed as per the manufacturer’s instructions (Promega). Luminescence was read using a Molecular Devices SpectraMax L plate reader.
-
bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
-
bioRxiv - Biophysics 2023Quote: ... transfection was carried out by adding 2 μg of freshly prepared bacmid DNA and 6 μL of FuGene HD transfection reagent (Promega, E2311), following the instructions provided by the manufacturer ...
-
bioRxiv - Genomics 2019Quote: ... Alternatively, ¼ of an agarose plug was soaked in GET solution (3% 2-mercaptoethanol, 0.5x QuantiFluor® dye (Promega), 1X TBE ...
-
bioRxiv - Molecular Biology 2020Quote: Cell viability was assayed with an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) colorimetric assay (Promega) per manufacturer’s instructions.