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Citations for Promega :
101 - 150 of 2095 citations for 6 Hydroxy 5 2 phenyldiazenyl 2 naphthalenesulfonic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: The therapeutic effect of free taxane (pro)drugs and LNP formulations was determined by the [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS)-based CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega) or the resazurin-based PrestoBlue assay (ThermoFisher) ...
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bioRxiv - Neuroscience 2022Quote: DNA constructs with different WT and mutated 5’UTRs of mouse Sox2 mRNA were assembled in the psiCheck-2 vector (Promega) including synthetic Renilla luciferase gene (hRluc ...
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bioRxiv - Biochemistry 2022Quote: ... 2ml of Sf9 cells at 0.5×106 cells per ml were transfected with 2 µg of fresh bacmid DNA and FuGene HD transfection reagent (Promega) at a ratio of 3:1 transfection reagent to DNA ...
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bioRxiv - Microbiology 2021Quote: ... An MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium]-based viability assay (CellTiter 96 aqueous nonradioactive cell proliferation assay, Promega) was performed as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mM KCl, 5 mM MgCl2, 2 mM DTT, 100 μg ml-1 cycloheximide, and 20 U ml-1 RNase inhibitor [Promega].) Gradients were centrifuged 36,000 rpm for 2.5 h in a SW41 Ti rotor (Beckman Coulter ...
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bioRxiv - Cell Biology 2022Quote: ... β-arrestin 2 fused at the N-terminus to LgBiT (LgBiT-β-arrestin 2; Promega, plasmid no. CS1603B118) was chosen as it has previously been used successfully with other class B GPCRs ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were diluted with 20 mM HEPES pH 8.0 to a urea concentration of 2 M and the proteins were digested with 4 µl Trypsin/LysC (Promega V5073: 20ug + 80uL 50mM acetic acid) for 4 hours at 37°C and boosted with an extra 2µl Trypsin/LysC (Promega V5073 ...
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bioRxiv - Cell Biology 2020Quote: ... 2 µl ViaFect™ (Promega, Madison, WI) and 40 µl Gibco Opti-MEM reduced serum media (Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: The psiCHECK-2 vector (Promega, Cat #C8021) was used to construct plasmids for dual-luciferase reporter assay ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mg/ml proteinase K (Promega, v3021)) at 37 °C overnight followed by addition of 10 μl of 3M potassium acetate and incubation at room temperature for 1h ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 µg of sequencing grade Trypsin (Promega) was added ...
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bioRxiv - Microbiology 2020Quote: ... and 2 μL RNasin RNase inhibitor (Promega). The transcription reaction was incubated overnight at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... and 2 μg Sequencing Grade Trypsin (Promega). The beads were shaken overnight at 37 °C and pelleted by centrifugation (1,400 rcf ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µg of sequencing grade trypsin (Promega) was then added to the samples and they were digested overnight at 37°C ...
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bioRxiv - Microbiology 2022Quote: The psiCHECK-2 control vector (Promega, C8021) and the psiCHECK-RL-Ifnb1 3′ UTR vector were described before26 ...
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bioRxiv - Microbiology 2021Quote: ... 2 µL dNTP (Promega U151B, 2.5 mM), 1.5 µL MgCl2 (25 mM) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 0.05 μg LgBiT-β-arrestin-2 (Promega) plus 0.9 µg pcDNA3.1 for 24 hours ...
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bioRxiv - Microbiology 2020Quote: ... 2 μL of luciferin reagent (Promega BrightGlo) was added and luciferase activity detected (Perkin Elmer Envision) ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 or 4 (Promega Madison, WI, USA) (62) ...
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bioRxiv - Bioengineering 2022Quote: ... 2 U/µL RNasin Ribonuclease Inhibitor (Promega), 1.2 µM PrimeTime primers ...
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Optimization and deoptimization of codons in SARS-CoV-2 and the implications for vaccine developmentbioRxiv - Evolutionary Biology 2022Quote: Dual-luciferase assay (psiCHECK-2 vector, Promega) was selected to analyze the effects of the viral mutation on protein synthesis ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We then added 2 μg trypsin (Promega) in 100 mM TEABC and incubated overnight at 30 °C ...
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bioRxiv - Microbiology 2023Quote: ... and 2× GoTaq qPCR Master Mix (Promega). qPCR was performed using the MyGo Pro real-time PCR instrument (IT-IS Life Science Ltd.) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... containing a 2× concentration of Endurazine (Promega) was added to each well ...
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bioRxiv - Molecular Biology 2024Quote: ... and digested with 2 µg Trypsin (Promega) overnight at 37 °C shaking at 1200 RPM on a thermomixer ...
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bioRxiv - Microbiology 2022Quote: Various KSHV mRNA 5’-UTRs containing uORFs were cloned up stream of the Renilla luciferase gene in a psiCHECK™-2 vector (Promega) using a Gibson Assembly® Cloning Kit (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2022Quote: To measure luciferase activity on plaque containing LB-agar plates, 5 μl of the diluted (1:50, in PBS) NanoLuc® luciferase substrate 2-furyl methyl-deoxy-coelenterazine (Furimazine; Promega) were dropped onto the respective plaques ...
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bioRxiv - Immunology 2020Quote: ... The following day cells were transfected with plasmids encoding for FLAG-tagged NLRP1 (2 μg) or the indicated DPP9 construct (2 µg) with FuGENE HD according to manufacturer’s instructions (Promega). Cells were harvested ...
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bioRxiv - Molecular Biology 2023Quote: ... precipitated proteins were eluted by incubation of beads with Elution Buffer (2 M Urea, 50 mM Tris pH 7.5, 2 mM DTT, 20 µg/ml Trypsin Gold (Promega)) for 30 min at 37°C shaking at 1,400 rpm in the dark ...
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bioRxiv - Biochemistry 2021Quote: ... Genomic DNA was eliminated by incubating 2 μg of RNA with 2 U of RQ1 RNase-free DNase I (Promega) for 30 min at 30°C ...
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bioRxiv - Genetics 2022Quote: ... Vectors coding for repair template DNA of roughly 2 kb were generated from Drosophila Schneider 2 cell genomic DNA by PCR using GoTaq G2 Polymerase (Promega) and primer pairs appropriate for the desired region (Supplementary Table S1) ...
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bioRxiv - Systems Biology 2020Quote: ... Reduced proteins were alkylated and trypsin-digested for 1-2 hours in trypsin solution (2 mg/ml sequencing grade porcine trypsin (Promega)/100 mM Tris/10 mM iodoacetamide ...
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bioRxiv - Microbiology 2022Quote: 293T cells were co-transfected with pEGFP-N1 and plasmids expressing SARS-CoV-2 Spike (FL) or SARS-CoV-2 Spike-Δ19 for 24 h using FugeneHD (Promega). The cells were treated with DMSO or 10 µM 6-TG at 4 h post- transfection ...
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bioRxiv - Biophysics 2019Quote: ... cells were treated with the LRRK2 inhibitor MLi-2 (100 nM) for 2 hours and then labelled with the TMR ligand (3 μM)(Promega). After 15 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were diluted to a concentration of urea of 2 mol L-1 and digested overnight with the addition of 2 µl sequencing-grade trypsin (Promega). After digestion ...
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bioRxiv - Molecular Biology 2023Quote: Proteins bound to beads were reduced with 2 mM DTT in 2 M urea buffer and sequencing grade trypsin (Promega) was added to a final concentration of 5 ng/μl ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then digested overnight with 2 µg of lysyl endopeptidase (Wako) at room temperature and further digested overnight with 2 µg trypsin (Promega) at room temperature ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... The pellet was washed with 2 ml nuclei storage buffer and resuspended in 0.2 ml PBS (calcium and magnesium-free) with 2% BSA and 0.2 U/μl RNasin® Plus RNase inhibitor (Promega). Buffer composition was described before72 ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested with 2 μg of lysyl endopeptidase (Wako) at RT for overnight and were further digested overnight with 2 μg trypsin (Promega) at RT ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized via reverse-transcription of 2-5 μg of RNA using M-MLV Reverse Transcriptase (200 U, Promega, Wisconsin, USA), random primers (10 ng/μl ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 2-5 ng of purified genomic DNA was spiked with 1% (w/w) of unmethylated cl857 Sam7 Lambda DNA (Promega, Madison, WI) and sheared to about 400bp using Covaris M220 (Covaris Inc. ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Bound proteins were eluted with elution buffer 1 (50 mM Tris-HCl pH 7.5, 2 M urea, 5 ng/µL sequencing grade modified trypsin (Promega, Madison, WI, USA), 1 mM DTT ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µl RNasin Plus (Promega, catalog no. N2615), 0.8 µl 100 mM ATP (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... using 2 µg of sequencing grade trypsin (Promega) per fraction 55 ...
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bioRxiv - Biochemistry 2022Quote: ... either 2 µg pGL4.47[luc2P/SIE/Hygro] (Promega) or pGL4.52[luc2P/STAT5RE/Hygro] (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... t-ERK1/2 (Promega V114A, 1/1000 dilution), p-AKT (Cell Signaling Technology ...
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bioRxiv - Immunology 2019Quote: ... with 2 µM Oligo(dT)15 primers (Promega) and 10 U RNase OUT in a 20 µl reaction at 37ºC for 90 min ...
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bioRxiv - Neuroscience 2019Quote: ... and 2 μg of sequence-grade trypsin (Promega). Samples were digested overnight in a 37 °C thermomixer (Eppendorf) ...
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bioRxiv - Molecular Biology 2021Quote: ... 2) pRL Renilla luciferase control reporter (Promega, E2241) and 3 ...
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bioRxiv - Cell Biology 2019Quote: ... 2° antibodies α- mouse (HRP detection, Promega, W4021). The mean intensity quantification of 4 independent experiments is shown in Figure 4 – supplement 1B ...