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Citations for Promega :
201 - 250 of 2237 citations for 6 Hydroxy 2 4 5 triaminopyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infectionbioRxiv - Immunology 2019Quote: HEK293 IFITM3−/− cells were plated in a 6-well dish for 24 hours prior to transfection of IFITM3-pcDNA3.1 plasmid DNA using FuGene 6 reagent (Promega) at a ratio of 3:1 ...
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bioRxiv - Cell Biology 2020Quote: ... washed again in DPBS and stained with 1mg/ml X-gal (5-bromo-4-chloro-3-indolyl-β-galactopyranoside, Promega, Madison, WI, US) in DMF (Dimetil formamide ...
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bioRxiv - Immunology 2020Quote: Activity of the inflammatory caspases 1/4/5 was measured using a commercially available Caspase-Glo® 1 Inflammasome Assay (Promega, WI, USA) from HFFs seeded in 96-well plates (2×104 cells per well) ...
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bioRxiv - Microbiology 2023Quote: ... and the reaction was revealed with a solution of NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate) (Promega, Madison, WI, USA).
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bioRxiv - Molecular Biology 2019Quote: ... purified by ethanol precipitation and co-transfected into Huh7 cells in 6-well plates (90 ng reporter, 10 ng pcDNA-Blast) using FuGENE 6 transfection reagent (Promega). Cells were selected with blasticidin (0.2 μg/mL ...
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bioRxiv - Neuroscience 2019Quote: ... in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were seeded in a 6-well plate and transfected with 750 ng of each of the AT1R-TANGO plasmids using FUGENE 6 Transfection (PROMEGA). The following day ...
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bioRxiv - Immunology 2020Quote: ... Plasmid transfections of IFITM1 3’ UTR reporter plasmids (500 ng per single well of a 6-well plate) were performed using the FuGENE 6 (Promega), according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... 1.5 μg of total DNA (1:10 ratio of the Cre plasmid to the fusion protein plasmid) were mixed with 6 μl of Fugene 6 transfection reagent (Promega) in 100 μl of Opti-MEM ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated on three 10 cm tissue culture dishes per condition and transfected 24 h before lysis using FuGENE 6 (6-12 μg total plasmid DNA; Promega). Published protocol can be found on Protocols.io (dx.doi.org/10.17504/protocols.io.kxygx3zeog8j/v1).
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bioRxiv - Microbiology 2024Quote: ... 4 μg of purified bacmid DNA were transfected into 1×106 Sf9 cells in a well of a 6-well plate using 6 μL of FuGENE HD transfection reagent (Promega). After 7 days of incubation at 27°C ...
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bioRxiv - Microbiology 2022Quote: ... the virus-induced cytopathogenic effect was measured colorimetrically by the formazan-based 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cell viability assay (CellTiter 96 AQueous One Solution Cell Proliferation Assay from Promega, Madison, WI), and the antiviral activity was expressed as the 50% effective concentration (EC50) ...
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bioRxiv - Microbiology 2019Quote: ... For transfection 0.5 or 2 µg of plasmid DNA in 25 or 200 µL serum-free medium were mixed with 1 or 4 µL of FuGENE HD transfection reagent (DNA to reagent ratio of 1:2, Promega, Mannheim, Germany) and incubated for 10 min at RT ...
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bioRxiv - Cancer Biology 2019Quote: ... The cell viability was assessed by the viability assays 3-(4,5-dimetiltiazol-2-il)-5-(3-carboximetoxifenil)-2-(4-sulfofenil)-2H-tetrazolio (MTS) using the Cell-Titer 96c AQueous Non-Radioactive Cell Proliferation Assay kit (Promega, Madison, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Various KSHV mRNA 5’-UTRs containing uORFs were cloned up stream of the Renilla luciferase gene in a psiCHECK™-2 vector (Promega) using a Gibson Assembly® Cloning Kit (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2022Quote: To measure luciferase activity on plaque containing LB-agar plates, 5 μl of the diluted (1:50, in PBS) NanoLuc® luciferase substrate 2-furyl methyl-deoxy-coelenterazine (Furimazine; Promega) were dropped onto the respective plaques ...
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bioRxiv - Microbiology 2019Quote: ... 5 μl of 5× Gotaq buffer (Promega M792A), 1μl of 10μM forward primer ...
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bioRxiv - Cell Biology 2020Quote: ... Transfections were carried out using Fugene 6 (Promega) at a ratio of 3µl reagent per µg DNA ...
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bioRxiv - Developmental Biology 2021Quote: ... for retroviruses using 20 µl FuGENE 6 (Promega) in 400 µl DMEM per plate ...
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bioRxiv - Molecular Biology 2019Quote: ... and were transfected using FuGENE® 6 (Promega). For each transfection ...
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bioRxiv - Developmental Biology 2019Quote: ... 293T cells were transfected using FUGENE 6 (Promega) following the manufacturer’s recommended protocol ...
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bioRxiv - Cell Biology 2020Quote: Plasmid transfection was performed using FuGENE 6 (Promega) according to the manufacturers’ protocol 24 hours before experiments whereas HiPerfect (Qiagen ...
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PPARγ is a tumor suppressor in basal bladder tumors offering new potential therapeutic opportunitiesbioRxiv - Cancer Biology 2019Quote: ... reporter plasmid and 6 ng pRL-SV40 (Promega), in the presence of the Fugene HD transfection reagent (Promega) ...
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bioRxiv - Genomics 2021Quote: ... plasmids using Fugene 6 Transfection Reagent (Promega, #E2311). 72 h after transfection ...
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bioRxiv - Cell Biology 2019Quote: ... Best results were obtained using Fugene 6 (Promega) as the transfection reagent but Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: ... for MEFs or Fugene 6 reagent (Promega, E269A) for HEK-293T cells ...
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bioRxiv - Microbiology 2021Quote: ... Cells were treated with 6 μM EnduRen (Promega), a substrate for Renilla luciferase (RL) ...
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bioRxiv - Molecular Biology 2022Quote: ... H9 hESCs were transfected using FuGENE 6 (Promega) with a targeting construct containing Blasticidin selection cassette ...
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bioRxiv - Cell Biology 2022Quote: The Fugene 6 transfection reagent (Promega, WI, USA) was used to transfect WT and mutant E and 3a plasmids for patch-clamp ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected using FuGene-6 (Promega #E2692) with the following constructs ...
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bioRxiv - Genomics 2023Quote: hCNCCs were transfected with FuGENE 6 (Promega, E2691) immediately after passaging ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were transfected with Fugene 6 (Promega). All other cell lines were transfected using Lipofectamine 2000 (ThermoFisher Scientific) ...
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bioRxiv - Physiology 2023Quote: ... the FuGENE 6 transfection reagent (Promega, WI, USA) was used to transfect wild-type (WT ...
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bioRxiv - Microbiology 2023Quote: ... 6 μL S30 premix without amino acids (Promega), plus water to a final reaction volume of 15 μL were incubated for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... into HEK293 using FuGENE 6 transfection reagent (Promega). Culture supernatant containing lentiviral particles was harvested after 24-48h incubation and passed through a 0.45 μm filter ...
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bioRxiv - Biochemistry 2023Quote: ... using the FuGENE®6 transfection reagent (Promega), and virus particles were harvested after 7 d of infection ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were transfected using FuGENE 6 (Promega) with complementary DNA (cDNA ...
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bioRxiv - Biochemistry 2024Quote: ... Fugene 6 was purchased from Promega (Madison, WI). Multiple plasmids were ordered or generated for this study ...
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bioRxiv - Cell Biology 2019Quote: ... then alkylation solution was removed and gel pieces were hydrated for 45 min at 4 °C in digestion solution (5 ng/μl trypsin, sequencing grade, Promega, in 25 mM AB). The trypsin digestion proceeded for 2 hours at 37 °C on Thermomixer (750 rpm ...
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bioRxiv - Cell Biology 2019Quote: ... then alkylation solution was removed and gel pieces were hydrated for 45 min at 4°C in digestion solution (5 ng/μl trypsin, sequencing grade, Promega, in 25 mM AB). The trypsin digestion proceeded for 2 hours at 37°C on Thermomixer (750 rpm ...
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bioRxiv - Immunology 2020Quote: ... After a migration time of 4 h at 37°C (5 % CO2) lower compartment were analyzed for cell content by Cell-Titer-Glo® assay (Promega, Madison, WI, USA). Percentages of migrated cells were calculated as described before45 and normalized to the CXCL12-only control.
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bioRxiv - Developmental Biology 2022Quote: ... Cells were plated in 24-well plate or 6-well plate and transfected next day using FuGENE® 6 (Promega, E2691). For luciferase assay ...
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bioRxiv - Microbiology 2022Quote: ... Transfection was achieved by adding 6 μg of plasmid DNA in 18 microliters of FuGENE® 6 Transfection Reagent (Promega # E2691); the obtained mixture was incubated in plain media for 20 minutes and then added to the pre-seeded culture dishes ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were transfected in a 6-well plate with 1 μg ADGRG6 DNA and 3 μL transfection reagent Fugene 6 (Promega, PRE2693) at 60-70% confluency and incubated for 48 h ...
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bioRxiv - Genomics 2020Quote: ... 4 µL RNase-A (4 mg-mL-1, Promega, Madison WI) was incubated at 37 °C for one hour vortexing every 15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: SMC5/6 hexamer purified from E.coli with a C-terminal HALO tag on Nse2 was incubated with a 2-fold molar excess of Janelia-Fluor646 HaloTag Ligand (Promega; 12 µM Smc5/6 hexamer + 24 µM label). After incubation for 1 h at 25°C ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized via reverse-transcription of 2-5 μg of RNA using M-MLV Reverse Transcriptase (200 U, Promega, Wisconsin, USA), random primers (10 ng/μl ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 2-5 ng of purified genomic DNA was spiked with 1% (w/w) of unmethylated cl857 Sam7 Lambda DNA (Promega, Madison, WI) and sheared to about 400bp using Covaris M220 (Covaris Inc. ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Bound proteins were eluted with elution buffer 1 (50 mM Tris-HCl pH 7.5, 2 M urea, 5 ng/µL sequencing grade modified trypsin (Promega, Madison, WI, USA), 1 mM DTT ...
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bioRxiv - Cell Biology 2021Quote: ... Briefly 70-90% confluent cells in 6-well plated were transfected with 1 µg CRISPR/Cas9 plasmids per well by using FuGENE 6 (Promega, Madison, Wisconsin). After 24 h post-transfection ...