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Citations for Promega :
351 - 400 of 1229 citations for 6 Ethoxypyridazin 3 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: SJ-GBM2 and SF8628 cells were used to determine if reducing WDR82 through inducible knockdown affected cell viability 1×104 cells/100μl were plated in 96-well plates with complete cell culture medium with or without Dox (2μg/ml), and subjected to 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, Promega) assay.
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bioRxiv - Cancer Biology 2019Quote: ... Sense (5’-AGGAAACTGAAAAACAGAGTAGCAGC-3’) and antisense (5’-TCCTTCTGGGTAGACCTCTGG-3’) primers were used in a standard GoTaq Green PCR reaction (Promega) to amplify a region spanning the 26-nucleotide intron that includes a single PstI restriction site ...
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bioRxiv - Genetics 2021Quote: ... A ~1 kb fragment of the blm cDNA was cloned using primers blm-F – 5’-GGAGTCGAAACACCTGGTGGTA-3’ and blm-R – 5’-CTCATCAATGACCAAGCGAGCC-3’ into pGEM-T vector (Promega) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Apoptosis was assessed by incubating the cells with 200nM Staurosporin or medium for 4 h and the caspase 3/7 activity was assayed using the ApoOne Caspase 3/7 Assay (Promega). Senescence associated β-Galactosidase activity was analyzed with the Senescence Associated β-Galactosidase Staining Kit (Cell Signalling Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... The V4 region of the 16S rRNA gene was amplified using the universal primers 515F (5′-GTG CCA GCM GCC GCG GTA A-3′) and 806R (5′-GGA CTA CNN GGG TAT CTA AT-3′) [26] with Taq&Load MasterMix (Promega). PCR reactions ...
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bioRxiv - Pathology 2021Quote: ... induction of apoptosis was determined by measurement of caspase-3/7 activity using the luminometric Caspase-Glo 3/7 assay (Promega) according to the manufacturer’s protocol and a microplate reader (Microplate Reader SH900) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were seeded on 24 mm glass coverslips and transfected with 2 µg plasmid DNA (1:1.3:3 ratio LAMP1:ER:opto-kinesin) and Fugene6 transfection reagent (Promega 1:3) for 20-30 h prior to imaging ...
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bioRxiv - Genomics 2022Quote: ... Caspase 3/7 activity was measured 16 and 24 h later using the Caspase-Glo 3/7 Assay System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a total of 18 RNA extractions (3 morphs × 3 tissues × 2 biological replicates) were performed using the SV Total RNA Isolation System (Promega) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... and digested with 3 μg LysC (Wako) O/N at 37°C and then with 3 μg of trypsin (Promega) for eight hours at 37°C following FASP procedure (Filter-aided sample preparation 48) ...
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bioRxiv - Microbiology 2023Quote: ... Caspase 3/7 enzymatic activity in raw cell lysates was measured using a Caspase Glo 3/7 assay kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... Apoptosis was measured by detecting Caspase 3/7 activity after 24 hours of treatment using the Caspase-Glo 3/7 Assay System (Promega) on a BMG CLARIOstar plate reader ...
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bioRxiv - Biochemistry 2022Quote: ... The 400 base pair region surrounding the sgRNA target was then amplified by PCR (forward primer: 5’-CCCAGAGAGGAGGCTGTAGA-3’; reverse primer: 5’-AAAGGCCTCCCAGGGGTTAT-3’) with GoTaq DNA Polymerase (Promega). The resulting PCR product was cloned into the pCR 4-TOPO vector using the TOPO TA Cloning Kit for Sequencing (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: RT reaction mix was set up and cDNA products were then amplified by PCR (25 cycles) with specific antigenome forward (5’-CATTCTACGAGCCGGTGCGC-3’) and reverse (5’-TAGACGTAGACCCCCAGAGTC-3’) primers using the GoTaq DNA polymerase (Promega) and analysed on a 1.5% agarose gel for analysis.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Initial P0 virus was obtained by addition of ∼3 µg recombinant bacmid DNA mixed with 3 µl FuGENE HD Transfection reagent (Promega) in 100 µl Sf900 II media (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... caspase-3/7 activities in cells were measured using a luminometric assay kit Caspase-Glo 3/7 (Promega, Madison, WI). The amount of luminescence was measured on the Victor3™ multilabel reader (PerkinElmer Inc.).
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bioRxiv - Cancer Biology 2024Quote: ... The activity of caspase 3/7 was measured after 48 h using the Caspase-Glo® 3/7 assay (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids encoding different Ocrl1 mutants were transfected using Fugene 6 reagent (Promega) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: DNA was transiently transfected into cells using FuGENE 6 transfection reagent (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: All ectopic transfections were performed using FuGENE® 6 Transfection Reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5-6ng pcDNA3.1 FLAG-cGAS and STING using 0.75μl FuGENE 6 (Promega) and 20μl Opti-MEM (Promega) ...
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bioRxiv - Developmental Biology 2021Quote: ... retroviral plasmid was transfected to HEK293 cells using FuGENE 6 (Promega, E2692). Two days after transfection ...
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PPARγ is a tumor suppressor in basal bladder tumors offering new potential therapeutic opportunitiesbioRxiv - Cancer Biology 2019Quote: ... 50 ng PPRE X3-TK-luc and 6 ng pRL-SV40 (Promega), in the presence of the Fugene HD transfection reagent (Promega) ...
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bioRxiv - Biochemistry 2022Quote: ... 1.2 µg p8.91 and 1.2 µg KAP1 pHRSIN using FuGENE 6 (Promega). Virus-containing supernatant was collected 48 h post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... Viral stocks were generated by transfecting 293T cells using Fugene 6 (Promega). After 48h ...
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bioRxiv - Microbiology 2022Quote: ... The DNA plasmid mix was added to 18µl of Fugene-6 (Promega) transfection reagent in 200µl of pre-warmed Opti-MEM (Gibco ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... Transfections and co-transfections were performed using FuGENE 6 transfection reagent (Promega). Cells were analysed two days post-transfection.
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bioRxiv - Cell Biology 2021Quote: ... HEK293T cells were transfected with plasmid DNA using Fugene 6 reagent (Promega) by following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2019Quote: ... cDNA transfections were performed using JetPEI (Polyplus Transfection) or Fugene 6 (Promega) transfection reagents according to manufacturers’ instructions for 24h ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.2 μg of pMD2.G envelope plasmid using FuGENE 6 (Promega). Cells were incubated up to 48 hrs and then lentivirus-containing media was collected and concentrated with Lenti-X concentrator (Clontech) ...
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bioRxiv - Microbiology 2021Quote: RSV VLPs were produced by FuGENE® 6 Transfection Reagent (Promega, E2691) transfection of DF1s at 50% confluence with 1 µg of viral plasmid ...
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bioRxiv - Immunology 2021Quote: ... and HIV Rev plasmid using Fugene 6 transfection reagent (Promega, Madison, WI). Virus-containing medium was titrated to ensure undersaturating conditions for infection of H1299 cells ...
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bioRxiv - Cell Biology 2022Quote: ... For transient transfection cells were incubated with mixture of FuGene 6 (Promega) and the plasmid of interest ...
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bioRxiv - Biochemistry 2022Quote: ... was mixed with 10 μL of FuGENE 6 transfection reagent (Promega E2693). After 5 minutes ...
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bioRxiv - Immunology 2022Quote: ... into 293T cells in DMEM medium + 10% FCS using Fugene 6 (Promega) for pseudoviruses production ...
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bioRxiv - Cancer Biology 2023Quote: ... along with the plasmid of interest using Fugene 6 transfection reagent (Promega). All shRNA plasmids used were purchased from Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... they were transfected with indicated plasmids using FuGENE 6 transfection reagent (Promega). After another 24h ...
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bioRxiv - Microbiology 2023Quote: ... in 50 µl RPMI 1640 medium with 6% low-IgG serum (Promega). After overnight incubation at 37°C in 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: Plasmid DNA transfections were performed using FuGENE® 6 transfection reagent (Promega). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected using a master mix of Fugene 6 (Promega, E269A) and Optimem (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells (70% confluent) were transfected using FuGene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Caspase 3/7 assay reagents (Promega, WI, USA) were added to each well according to the manufacturer’s instructions (ratio of 1:4) ...
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bioRxiv - Molecular Biology 2021Quote: ... 3’-UTR derived from pNL1.1[Nluc] vector (Promega), and 50 nt poly(A ...
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bioRxiv - Immunology 2021Quote: ... 3) goat anti-human H+L (Promega, W403B) at 1:3,000 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... Caspase-Glo® 3/7 Assay System (Promega) was used according to manufacturer’s guidelines ...
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bioRxiv - Systems Biology 2020Quote: ... and T4 DNA ligase (3 U/μί, Promega) were applied to assemble all of the synthetic promoter blocks sequentially and simultaneously into the firefly reporter vector backbone in a one-pot reaction ...
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bioRxiv - Cancer Biology 2020Quote: Caspase 3/7 Assay kit (Promega, Southampton, UK) was utilized to assess apoptosis as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Caspase-Glo 3/7 assay (Promega, Madison, WI) (5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Caspase-Glo 3/7 Assay (Promega, Cat# G8090) reagent was added to the cells and incubated for 30 min ...
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bioRxiv - Bioengineering 2022Quote: ... by Caspase-Glo® 3/7 Assay (Promega) using GloMax Discover Microplate Reader (Promega).