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Citations for Promega :
701 - 750 of 3596 citations for 6 Chlorotetrazolo 1 5 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Beads were washed 6 times with 50 mM ammonium bicarbonate and then treated with TPCK-treated modified trypsin (Promega) for 16 hours at 37°C on an end-over-end rotator ...
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Loss of intermicrovillar adhesion impairs basolateral junctional complexes in transporting epitheliabioRxiv - Cell Biology 2024Quote: A validated CDHR2 KO CL4 cell clonal population was transfected with pHALO-N3-CDHR2 using FuGENE 6 (Promega #E2691) at a FuGENE:DNA (μL:μg ...
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bioRxiv - Microbiology 2021Quote: ... and the near full-length 16S rRNA gene was amplified using primers 8F (5’-AGAGTTTGATCCTGGCTCAG-3’) and 1492R (5’-TACGGTTACCTTGTTACGA-3’) with the GoTaq® Hot Start Colorless Master Mix (Promega Corp., Madison, WI, USA). PCR was performed using Eppendorf Vapo Protect Mastercycler Pro S 6325 (Hamburg ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μL of CellTiter-Fluor Cell Viability Assay reagent (Promega) was added to each well ...
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bioRxiv - Microbiology 2019Quote: ... 0.5 µl of 5 unit Taq Polymerase (Promega, Madison, WI, USA) and 2.5 µl of DNA template ...
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bioRxiv - Immunology 2019Quote: ... and the luminescence after incubation with 5 µM coelenterazine H (Promega) were recorded using a Mithras LB940 reader (Berthold).
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bioRxiv - Genomics 2020Quote: ... containing 5 μL of GoTaq Master Mix (Promega, Mdison, WI, USA), 1 mM each of forward and reverse primer ...
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bioRxiv - Plant Biology 2021Quote: ... 5 μg/mL Mass Spec Trypsin/Lys-C Mix (V5073, Promega) and 1 mM DTT was added to the samples ...
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bioRxiv - Molecular Biology 2022Quote: ... or 5-10% (w/vol) PEG8000 (Promega; radius of gyration (Rg) ∼3-5 nm (Cole & Ralston ...
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bioRxiv - Molecular Biology 2019Quote: ... 5% CO2 and cell viability was measured using CellTiter-Glo (Promega) as per the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... and 5 ng/ml recombinant human fibroblast growth factor (G5071, Promega).
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bioRxiv - Plant Biology 2019Quote: ... seedlings were sprayed with a 5 mM D-Luciferin (Promega, USA), 0.01 % Triton X-100 solution ...
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bioRxiv - Microbiology 2019Quote: ... 5 μl of 2× GoTaq q-PCR master mix (Promega, USA) and 0.6 μM of AF77/78 or AF79/80 primer pairs targeting a region close to the Cori or to the terminus (ter) ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were digested with 5 μg of trypsin (Promega, Cat# V5280) at 37 °C overnight under agitation ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM EDTA) supplemented with 1X Protease inhibitor cocktail (Promega G6521) and PhosSTOP phosphatase inhibitor (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µl of 50 pmol/µL biotinylated oligo-dT probes (Promega), and 408 µl of diffusion buffer (3X Saline-Sodium Citrate (SSC) ...
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bioRxiv - Microbiology 2023Quote: ... cells were treated with 5 μM Z-VAD-FMK (Promega G7231) or DMSO vehicle control ...
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bioRxiv - Microbiology 2023Quote: ... and then 50 μl of 5% (v/v) SteadyGlo reagent (Promega) in glycine buffer (50 mM glycine ...
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bioRxiv - Microbiology 2023Quote: ... 5 μM 11S and 2 μl furimazine solution (Promega Cat. # N1610) were added and the luminescence read at 1 min intervals for 10 min using the FLUOstar Omega using a gain value of 3,000 ...
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bioRxiv - Biochemistry 2023Quote: ... 5% polyacrylamide TBE gel and stained with Diamond DNA stain (Promega). Experiments were performed with H ...
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bioRxiv - Neuroscience 2023Quote: ... we added RNasin Plus (5% of final volume, Promega, Madison, WI). After immunostaining ...
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bioRxiv - Plant Biology 2023Quote: ... 0.25 µL of GoTaq® DNA Polymerase (5 U/µL, Promega) and 28.25 µL of ultra-pure water were added into the tubes ...
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bioRxiv - Plant Biology 2023Quote: ... 0.25 µL of GoTaq® DNA Polymerase (5 U/µL, Promega), and 27.75 µL of ultra-pure water ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293TN cells were seeded in 6-well plates and transiently transfected with plasmid of choice using FuGene transfection reagent (Promega) and OptiMem (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... S2-CP8 cells were then transiently transfected with both mRFP-FKBP-5-ptase-dom and PM-FRB-CFP (0.5 μg/well of a 6-well plate for each vector) with ViaFect (Promega Corp.). After 24 h culture ...
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bioRxiv - Cell Biology 2019Quote: Glucose uptake was measured based on the detection of 2-deoxyglucose-6-phosphate uptake by a commercially available luminescence-based kit (Glucose Uptake-GloTM Assay, Promega) on a SpectraMax M3 (Molecular Devices) ...
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bioRxiv - Neuroscience 2021Quote: ... coated 12-well plate and transfected the next day using the Fugene 6 transfection reagent and according to the manufacturer’s directions (Promega E2691). The same amount of plasmid was transfected per condition ...
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bioRxiv - Neuroscience 2021Quote: ... 100 mm cell culture dishes were seeded with cells 24 hours prior to transfection and transfected the following day with the pCAGIG-Emx2 plasmid using FuGENE 6 transfection reagent (Promega) as per the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... FASP was carried out in two 10 kDa MWCO filters with a 50 mM iodoacetamide alkylation step and proteins were digested in 2M urea with 2% wt/wt Lys-C (Wako) for 6 h and 2% modified trypsin (Promega) for 12 h at 37°C ...
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bioRxiv - Biochemistry 2022Quote: HeLa and HEK293T cells were seeded on 18 mm glass coverslips at a density of 3×105 cells/mL and were transfected with FuGENE 6 (Promega) transfection reagent 24 h later ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 ng of lentiviral plasmid encoding doxycycline inducible transporters and 500 ng of each of the packaging plasmids were transfected into HEK 293T cells with FuGENE 6 transfection reagent (Promega). Cell supernatant was replaced 24 h after transfection and harvested after another 24 h ...
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bioRxiv - Molecular Biology 2021Quote: RNA extraction from liver tissue (n=3-6 biological replicates per group) was performed using the ReliaPrep RNA Miniprep system (Promega), as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Transfections were performed with 4 μg of DNA, 800 μL of OptiMEM (Invitrogen, and 12 μL of FuGENE 6 transfection reagent (Promega) per 10 cm dish ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were then stimulated with 1 ng/mL of IL-1β for 6 h and subsequently washed with ice-cold PBS and lysed in Passive Lysis Buffer (PLB; Promega). Luciferase activity was measured in a Clariostar plate reader (BMG Biotech ...
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bioRxiv - Neuroscience 2019Quote: ... the whole brain of postnatal day 6 (P6) animals or dorsal raphe tissue punches (adult animals) were homogenized with Passive Lysis Buffer (Promega) and proteinase inhibitors (cOmplete ULTRA Tablets ...
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bioRxiv - Biochemistry 2019Quote: ... 500 ng of the lentiviral backbone plasmid containing the guide sequence and 500 ng of each of the packaging plasmids were transfected into HEK 293T cells using FuGENE 6 transfection reagent (Promega). The viral media was exchanged after 24 hours and was harvested after 48 hours ...
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bioRxiv - Biochemistry 2019Quote: ... These cells were then transfected with 50 ng of pcDNA3-STING-HA and 500 ng of either pcDNA3-FLAG-HA or pcDNA3-FLAG-HA-SLC19A1 using FuGENE 6 transfection reagent (Promega). After 24 h ...
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bioRxiv - Cancer Biology 2019Quote: Replication-deficient retro- and lentiviral particles were produced by transient transfection of 293T cells using FuGENE® 6 reagent (Promega). In brief ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293T cells were grown in two 175 cm2 flasks to ∼50% confluence and transfected with 25 μg plasmid and 75 μl FuGENE 6 in 2 ml Opti-MEM according to the manufacturers instructions (Promega). One day after transfection ...
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bioRxiv - Biochemistry 2020Quote: ... 500 ng of the lentiviral backbone plasmid containing the guide sequence and 500 ng of each of the packaging plasmids were transfected into HEK 293T cells using FuGENE 6 transfection reagent (Promega). The viral media was exchanged after 24 hours ...
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bioRxiv - Neuroscience 2020Quote: ... and a pFUGW vector containing a Cre-recombinase and/or vGluT1-pHluorin construct using the Fugene 6 transfection reagent (Promega). Cell culture supernatants containing the virus were collected 72 hours later and spun down to precipitate out cellular debris and other contaminants ...
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bioRxiv - Microbiology 2021Quote: ... or chimeric infectious clone plasmid was transfected into C6/36 or 293T cells by FuGENE 6 transfection reagent (Promega, USA). At 72 h post-transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were re-solubilized in 6 M urea buffer followed by reduction and alkylation before digestion with trypsin (Promega) at 37°C for 18 hr ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293T cells were seeded in 24-well tissue culture plates at a density of 1.6 x 105 cells/well and transfected with 50-100 ng/well of pNL4-3-GFP using Fugene HD (Promega) transfection reagent ...
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bioRxiv - Biophysics 2020Quote: ... We stained the cells with 3-6 pM Halo-SiR ligand 48 directly before imaging according to the protocol of the manufacturer (Promega) to obtain equal molecular densities during imaging and therefore minimize differences in tracking losses.
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bioRxiv - Biophysics 2020Quote: ... mass ratio) into LentiX cells grown to approximately 80% confluency in 6-well plates using FuGENE HD Transfection Reagent (Promega) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... RPE1 wild type cells were transfected with PX459 plasmid containing the corresponding guide sequence for 24 hours using FuGENE 6 (Promega). Following 72 hours of 4 μM Puromycin selection ...
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bioRxiv - Molecular Biology 2022Quote: ... were co-transfected with psPAX2 and pCMV-VSV-G packaging plasmids into HEK293T cells using FuGENE 6 (Promega, WI, USA). Supernatants containing virus were collected at 48h and 72h after transfection ...
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Combining LSD1 and JAK-STAT inhibition targets Down syndrome-associated myeloid leukemia at its corebioRxiv - Cancer Biology 2022Quote: ... Cell viability was determined on day 6 using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega, Madison, WI, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 6 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:25 (w/w ...