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Citations for Promega :
301 - 350 of 4317 citations for 6 Chloropyrazolo 1 5 a pyrimidine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: hCNCCs were transfected with FuGENE 6 (Promega, E2691) immediately after passaging ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were transfected with Fugene 6 (Promega). All other cell lines were transfected using Lipofectamine 2000 (ThermoFisher Scientific) ...
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bioRxiv - Physiology 2023Quote: ... the FuGENE 6 transfection reagent (Promega, WI, USA) was used to transfect wild-type (WT ...
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bioRxiv - Biochemistry 2023Quote: ... using the FuGENE®6 transfection reagent (Promega), and virus particles were harvested after 7 d of infection ...
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bioRxiv - Cancer Biology 2023Quote: ... into HEK293 using FuGENE 6 transfection reagent (Promega). Culture supernatant containing lentiviral particles was harvested after 24-48h incubation and passed through a 0.45 μm filter ...
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bioRxiv - Biochemistry 2024Quote: ... Fugene 6 was purchased from Promega (Madison, WI). Multiple plasmids were ordered or generated for this study ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were transfected using FuGENE 6 (Promega) with complementary DNA (cDNA ...
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bioRxiv - Systems Biology 2023Quote: ... 5 x 106 cells were electroporated with 1 μg of NanoLuc pNL3.1 (Promega, N1031) vector and 0.2 μg of control vector ...
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bioRxiv - Evolutionary Biology 2024Quote: ... for 30 minutes then with 5 units/1 L RQ1 RNase-free DNase (Promega) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were rinsed 5 times for 8 min in PBS-tween 0.2% and incubated with secondary antibodies for 2 h at room temperature under agitation (HRP anti-mouse, 1:10000, Promega cat. # W4021). Membranes were further rinsed 5 times for 8 min in PBS-tween 0.2% ...
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bioRxiv - Genetics 2021Quote: Senescence was measured using 2 assays: 1/ Beta-Glo Assay kit (Promega # E4720), according to the manufacturer’s instructions and utilizing a luciferin-galactoside substrate (6-O-β galactopyranosylluciferin) ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM CaCl2) and incubated for 4 hours with 1 µg trypsin (Promega) at 37°C and shaking at 1200 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transiently transfected with 1 or 2 µg HaloTag-Ubiquitin (N2721, Promega) or the control vector pHTN HaloTag CMV-neo (G7721 ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 μM methionine-free amino acid mixture (Promega). The Click-iT reaction was performed with the Click-&-Go Protein Reaction Buffer Kit (Click Chemistry Tools ...
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bioRxiv - Systems Biology 2019Quote: ... and 50 μM of each amino acid (Promega, #L446A). T7 RNA polymerase (NEBs ...
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bioRxiv - Cell Biology 2021Quote: ... 14 μM amino acid mix w/o methionine (Promega)) ...
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bioRxiv - Neuroscience 2023Quote: ... and stained in Diamond Nucleic Acid Stain (Promega, H1181). All experiments were performed in 8-12 week old animals ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were plated in 24-well plate or 6-well plate and transfected next day using FuGENE® 6 (Promega, E2691). For luciferase assay ...
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bioRxiv - Microbiology 2022Quote: ... Transfection was achieved by adding 6 μg of plasmid DNA in 18 microliters of FuGENE® 6 Transfection Reagent (Promega # E2691); the obtained mixture was incubated in plain media for 20 minutes and then added to the pre-seeded culture dishes ...
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bioRxiv - Immunology 2023Quote: ... samples were diluted 1:1 with water and digested for 1.5 hours at 37 °C with 1 µg of LysC and overnight at 37 °C with 1 µg trypsin (Promega). The peptide mixture was acidified with trifluoroacetic acid (Merck ...
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bioRxiv - Neuroscience 2023Quote: ... UDP (at concentrations ranging from 1 nM to 1 mM) was then applied with a final concentration of 5 μM furimazine (NanoLuc Luciferase Assay, Promega). Luminescence was subsequently measured using a Victor X5 multi-label plate reader (PerkinElmer).
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bioRxiv - Molecular Biology 2023Quote: SMC5/6 hexamer purified from E.coli with a C-terminal HALO tag on Nse2 was incubated with a 2-fold molar excess of Janelia-Fluor646 HaloTag Ligand (Promega; 12 µM Smc5/6 hexamer + 24 µM label). After incubation for 1 h at 25°C ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized via reverse-transcription of 2-5 μg of RNA using M-MLV Reverse Transcriptase (200 U, Promega, Wisconsin, USA), random primers (10 ng/μl ...
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bioRxiv - Biochemistry 2019Quote: ... The samples were then diluted with 150 μL PBS and to this was added 1 mM CaCl2 (2 μL of a 200 mM stock in water) and trypsin (2 μg, Promega, sequencing grade, V5111) and the digestion was allowed to digest overnight at 37 °C with shaking ...
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bioRxiv - Molecular Biology 2020Quote: ... 150 mM NaCl, 1 mM MgCl2, 2 mM EDTA, 1% NP-40, 1 mM DTT, 100 U/mL RNasin [Promega], 1X protease inhibitor) at 7 dpi ...
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bioRxiv - Cell Biology 2020Quote: ... 250 ng of each of the donor and Cas9-sgRNA vectors were transfected to RPE-1 cells cultured in 6 wells with 1.5 μl of FuGENE® HD (Promega, E2311) overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected the following day using 0.6–1 µg of plasmid and Viafect reagent (Promega E4981, 6 µl/µg plasmid). The medium was exchanged the following day to regular culture medium ...
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bioRxiv - Molecular Biology 2022Quote: ... terminated by incubation on ice and diluted 1:5 in Glo Lysis Buffer (Promega # E2661). 25 μL of prepared Nano-Glo reagent (Promega # N1120 ...
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bioRxiv - Biochemistry 2023Quote: ... with 25 μL of diluted reactions (diluted 1:5 in Glo Lysis Buffer; Promega # E2661) and incubated at room temperature for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with 36 µL FuGENE 6 (Promega) and 12 µg of retroviral plasmid DNA ...
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bioRxiv - Cell Biology 2019Quote: ... whereas SKBr3 cells were transfected using Fugene 6 (Promega), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Transfection was performed with FuGENE 6 transfection reagent (Promega). All the cell lines were regularly confirmed to be free of contamination (e.g ...
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bioRxiv - Molecular Biology 2021Quote: HEK-293T cells were transfected using Fugene 6 (Promega), Lipofectamine LTX ...
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bioRxiv - Cell Biology 2019Quote: ... Digestion was with 6 ng/μl trypsin (Promega, UK) overnight at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... and 6 ng/μl of sequencing grade trypsin (Promega) for 1 h at 50 °C ...
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bioRxiv - Cell Biology 2021Quote: ... pKIF1CP176L-GFP or pKIF1CR169W-GFP using Fugene 6 (Promega) in fibronectin-coated glass-bottom dishes and imaged 24 hours later on an Olympus Deltavision microscope (Applied Precision ...
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bioRxiv - Cell Biology 2021Quote: hTert-RPE1 cells were transfected using FuGENE 6 (Promega) according to the manufacturer’s instructions at a ratio of 1:3 ...
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bioRxiv - Cancer Biology 2021Quote: ... or the FuGENE 6™ (Promega, Fitchburg, WI, USA) transfection reagents according to the suppliers’ instructions for 1-2 days before the experiments ...
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bioRxiv - Neuroscience 2022Quote: ... then 6 μL FuGENE HD Transfection Reagent (Promega, USA) was added and vortexed to obtain a 3:1 reagent-to-DNA ratio ...
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bioRxiv - Immunology 2022Quote: ... by transfection using Fugene 6 (Promega, catalog no. E2692) and a combination of S plasmid ...
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bioRxiv - Immunology 2022Quote: ... and p MD.2G using Fugene 6 (Promega, PAE2693) in HEK293T cells ...
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bioRxiv - Genetics 2022Quote: ... The transfection mix was prepared using Fugene 6 (Promega) at 3µl/ug DNA in 500µl (total volume ...
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bioRxiv - Bioengineering 2020Quote: ... and 42 μL FuGENE 6 (Promega, Cat. No. E2691). The tube was gently flicked to mix the plasmids before and after the addition of FuGENE 6 ...
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bioRxiv - Biochemistry 2020Quote: ... Transfections were carried out by using Fugene 6 (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... or FLAG-KrascomQ61R using FuGene 6 reagent (Promega, E2691) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... HEK293 cells were transiently transfected using Fugene 6 (Promega) with GFP-Tnf 3’UTR reporter constructs and a pGL3-mCherry control construct ...
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bioRxiv - Cell Biology 2021Quote: ... Digestion was with 6 ng/μl trypsin (Promega, UK) overnight at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected using FuGENE 6 (Promega, Madison, WI) or lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and 18 μl of FuGENE 6 transfection reagent (Promega), according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected using FuGENE® 6 reagent (Promega) combined with 12 μg of the indicated plasmid cDNA construct ...