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Citations for Promega :
301 - 350 of 1747 citations for 6 Bromo 2 4 chlorophenyl quinoline 4 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Trypsin (6 ng/ml, Promega) in NH4HCO3 (50 mM ...
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bioRxiv - Microbiology 2021Quote: ... using FuGENE 6 (Promega, # E2311). 40 hours post-transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... FuGENE 6 Transfection Reagent (Promega) was used for DNA transfection ...
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bioRxiv - Microbiology 2020Quote: ... or Fugene 6 (Promega, UK) and virus containing supernatants harvested 3 days later ...
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bioRxiv - Neuroscience 2021Quote: ... or Fugene® 6 (Promega) in a 3:1 ratio with cDNA mix ...
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bioRxiv - Cancer Biology 2021Quote: ... 6 μl of FuGene (Promega) transfection reagent was added ...
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bioRxiv - Microbiology 2020Quote: ... using Fugene 6 (Promega, # E2311). Variants of plasmids encoding spike included the wild-type pCMV-S ...
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bioRxiv - Cell Biology 2022Quote: ... using Fugene 6 reagent (Promega). The culture supernatant was centrifuged ...
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bioRxiv - Microbiology 2023Quote: ... using FuGene®6 (Promega) according to the manufacturer’s instructions (Fodor et al. ...
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bioRxiv - Biochemistry 2021Quote: ... The residual magnetic beads were then incubated for two hours at 4°C and for one hour at 16°C in 83 microlitres of buffer A200 with 20 Units RNasin (Promega), 12mM DTT and 16 micrograms of TEV-Protease ...
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bioRxiv - Genomics 2021Quote: ... RNA concentration was measured in a DeNovix DS-11 spectrophotometer (DeNovix Inc, USA) and 4 μg of RNA were subjected to DNAse treatment (RQ1 - Promega) according manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: Plasmid DNAs containing Tol2 vectors comprising GV genes and fluorescent protein genes described above and transposase mRNA were introduced to KPL-4 cells by lipofection using FuGene HD (Promega) in OptiMEM (Gibco) ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were digested with 0.5 µg of lysyl endopeptidase (Wako) at room temperature for 4 hours and were further digested overnight with 1 µg trypsin (Promega) at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated at 37°C for 1 hour and then proteolytic digestion was performed with LysC (Wako) for 4 hours and trypsin (Promega) overnight ...
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bioRxiv - Cell Biology 2019Quote: ... The reaction was diluted 1:4 with 50 mM ammonium bicarbonate and incubated with 1 µg of Trypsin Gold (Promega) at 37°C for more than 18 h before desalting ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-m6A conjugated beads were incubated with purified mRNA with rotation at 4°C overnight in 300 µL MeRIP buffer with 1 µL RNase inhibitor (recombinant RNasin; Promega). 10% of the mRNA sample was saved as the input fraction ...
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bioRxiv - Synthetic Biology 2019Quote: ... Backbone and inserts were ligated either for 3 h at RT or overnight at 4°C using T4 ligase (Promega). All vector sequences (Table S5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The sequences ligated by the T4 ligase at 4 °C for 12 h were cloned in the pGEM T-easy vector (Promega). The competent Escherichia coli X10-Gold (Stratagene ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells cultured in 24-well plates were transfected with indicated firefly luciferase reporter construct (SBE×4-luc or BRE-luc) together with pRL-SV40 renilla luciferase control vector (Promega) using Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Twenty nanograms of DNA were used as a model for 18 cycles of amplification of the V4 region of the 16S rRNA gene, using primers 515F and 806R (Caporaso, et al. 2012: 1621-4) and GoTaq Master Mix (Promega). PCR products were quantified with the Qschd dsDNA HS kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... After 30 min at 60°C the samples were cooled to room temperature and 4 μg of Sequencing Grade Trypsin (Promega) for digestion were added ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Recombinant baculovirus was produced by transfecting recombinant bacmids (2-3 μg) into Sf9 cells (5 mL, density of 4×105 cells per mL) using FuGENE HD Transfection Reagent (Promega) and Opti-MEM Reduced Serum Media (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... Cross-links of input and IP material were reversed by adding 10 μl 5 M NaCl and 4 μl 10 mg ml−1 Proteinase K (Promega), and incubated for 4 hours at 65°C ...
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bioRxiv - Cell Biology 2021Quote: ... Washed beads were re- suspended in 150 µl digestion buffer and incubated for 4 hours with 1 µg trypsin (Promega) at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... 8 min, 4 °C) and resuspended in staining buffer (PBS, pH 7.4, with 0.5% RNase-free BSA (Promega, Heidelberg, Germany) and 0.2 U/μl of RNasin Plus RNase inhibitor (Promega)) ...
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bioRxiv - Genomics 2022Quote: ... The pRF+423Dux 4 vector was sequenced using primers that anneal to the pGL3 vector (Promega, see primers Table S2). The Dux4 IRES site was deleted from pRF+423Dux4 using PCR primers that flank the IRES region (PRF423DUX4-ATW F and R ...
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bioRxiv - Cancer Biology 2022Quote: ... 25,000 leukemia cells in 250µL of drug-containing medium were seeded in a 48-well plate and viability was assessed every 4 days by using the CellTiter-Glo Luminescent Cell Viability assay (Promega). For RNA-Seq and ChIP-Seq experiments ...
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bioRxiv - Biochemistry 2022Quote: ... Gel pieces were then rehydrated in 50 ul 50 mM ammonium bicarbonate containing 4 ng/µl trypsin (Promega, Madison, WI) and 0.01% proteaseMAX (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... lysyl endopeptidase (Wako) at room temperature for 4 hrs and were further digested overnight with 1:50 (w/w) trypsin (Promega) at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and Bone Morphogenetic Protein 4 (BMP4, 10 ng/ml, Bio-Techne) plus phosphoinositide 3-kinase inhibitor Ly294002 (10 µM, Promega). After 42 h incubation at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were resolved in reducing and non-reducing SDS-PAGE gels and membranes were incubated overnight at 4°C with the following antibodies: rabbit polyclonal anti-human p75 intracellular domain (1:1000, Promega); mouse monoclonal anti-HA (1:2000 ...
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bioRxiv - Microbiology 2022Quote: ... The resultant plasmid and Bsu36I-digested BmNPV-abb genome DNA [47] were cotransfected into BmN-4 cells using FuGeneHD (Promega). The recombinant virus was purified twice using the plaque assay by identifying OB-producing plaques ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 4 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:250 (w/w ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was performed using 1:100 (w/w) LysC (Wako Chemicals) for 4 h at 37°C and then finalised with 1:50 (w/w) trypsin (Promega) overnight at 37°C ...
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bioRxiv - Cell Biology 2020Quote: IC50 was determined on days 3 and 4 after vemurafenib treatment using Real-time-Glo MT Cell Viability Assay according to the manufacturer’s protocol (Promega, #G9711). Using a 96-well white plate ...
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bioRxiv - Molecular Biology 2020Quote: ... Apoptosis was assessed by incubating the cells with 200nM Staurosporin or medium for 4 h and the caspase 3/7 activity was assayed using the ApoOne Caspase 3/7 Assay (Promega). Senescence associated β-Galactosidase activity was analyzed with the Senescence Associated β-Galactosidase Staining Kit (Cell Signalling Technologies) ...
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bioRxiv - Plant Biology 2021Quote: ... Treatment with Proteinase K was carried out in 500 μl of PK Buffer (100 mM Tris-HCl pH8; 50 mM NaCl; 10 mM EDTA; Proteinase K 4 mg/ml; RNasin PROMEGA) 2 h at 55 °C and 15 min at 95 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Three washes were performed with ChIP Dilution Buffer as in DRIP assays before incubating the IP fraction with Proteinase K for 2 h at 55°C plus 15 min at 95°C in PK Buffer (100 mM Tris-HCl pH8; 50 mM NaCl; 10 mM EDTA; 4 mg/ml Proteinase K; RNasin PROMEGA). We added 2 U DNase I (ThermoFisher ...
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bioRxiv - Cell Biology 2019Quote: ... The proteins were digested in less than 4 M urea by endoproteinase LysC (Wako Chemicals) followed by digestion with Trypsin Gold (Promega) in less than 2 M urea ...
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bioRxiv - Cancer Biology 2020Quote: Selected protein spots were excised from the gel and enzymatically digested in-gel essentially as previously described(4) using trypsin (Promega). Gel pieces were washed with 50% acetonitrile to remove SDS ...
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bioRxiv - Cancer Biology 2021Quote: ... assessed every day for 4 days by measuring ATP content through Cell Titer-Glo® Luminescent Cell Viability assay (Promega), was compared to cell viability assayed at day 1 ...
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bioRxiv - Immunology 2020Quote: ... Single CD3-CD8-CD16- CD20+Ova-RBD-PE+RBD-AF647+ B cells were sorted into individual wells of a 96-well plates containing 4 μl of lysis buffer (0.5 X PBS, 10mM DTT, 3000 units/mL RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III (Becton Dickinson) ...
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bioRxiv - Cell Biology 2022Quote: ... peptides were diluted 4-fold with ABC buffer (Ammonium bicarbonate 50 mM pH8) and digested with Trypsin MS grade (Promega) overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligos were then ligated at 4°C overnight to the appropriate digested ACR-16-REM sequence (T4 ligase, Promega, USA) and transformed into DH5α (ThermoScientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were passaged every 3–4 days and regularly checked for mycoplasma infections using a GoTaq G2 Hot Start Taq Polymerase kit from Promega.
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bioRxiv - Immunology 2023Quote: ... total RNA was purified from 3-4 tissues of female flies using a ReliaPrep RNA Tissue Miniprep kit (Promega, z6112). Quantitative RT-PCR was performed using a OneTaq RT‒PCR kit (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... For in vitro import assay WT and MLS-TFEB cDNA were cloned into the pGEM-4 vector and transcribed in vitro using the TNT Quick coupled translation system (L1170, Promega), by incubating 200 ng of DNA with 7 μL of TNT-T7 reticulocyte lysate mix and 0.5 µL of 35S-labelled methionine (NEG709A500UC ...
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bioRxiv - Neuroscience 2023Quote: ... the washed beads were re-suspended in 150 µl trypsin digestion buffer and incubated for 4 hours with 1 µg trypsin (Promega) at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... S2R+ cells were grown in SF-4 medium and cotransfected with desired plasmids using FuGENE® HD transfection reagent (Promega). Specifically ...
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bioRxiv - Biochemistry 2023Quote: ... Selected gel bands that did not bind to the 4’-methylgenistein beads were subjected to in-gel digestion with trypsin (MS grade; Promega). Peptide fragments were analyzed using a Q-TOF/MS ...