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Citations for Promega :
451 - 500 of 4483 citations for 6 BROMO 4 4 DIETHYL 1H BENZO D 1 3 OXAZINE 2 4H THIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Gel pieces were then rehydrated in 50 ul 50 mM ammonium bicarbonate containing 4 ng/µl trypsin (Promega, Madison, WI) and 0.01% proteaseMAX (Promega ...
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bioRxiv - Microbiology 2022Quote: ... The resultant plasmid and Bsu36I-digested BmNPV-abb genome DNA [47] were cotransfected into BmN-4 cells using FuGeneHD (Promega). The recombinant virus was purified twice using the plaque assay by identifying OB-producing plaques ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 4 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:250 (w/w ...
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bioRxiv - Cell Biology 2020Quote: IC50 was determined on days 3 and 4 after vemurafenib treatment using Real-time-Glo MT Cell Viability Assay according to the manufacturer’s protocol (Promega, #G9711). Using a 96-well white plate ...
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bioRxiv - Plant Biology 2021Quote: ... Treatment with Proteinase K was carried out in 500 μl of PK Buffer (100 mM Tris-HCl pH8; 50 mM NaCl; 10 mM EDTA; Proteinase K 4 mg/ml; RNasin PROMEGA) 2 h at 55 °C and 15 min at 95 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Three washes were performed with ChIP Dilution Buffer as in DRIP assays before incubating the IP fraction with Proteinase K for 2 h at 55°C plus 15 min at 95°C in PK Buffer (100 mM Tris-HCl pH8; 50 mM NaCl; 10 mM EDTA; 4 mg/ml Proteinase K; RNasin PROMEGA). We added 2 U DNase I (ThermoFisher ...
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bioRxiv - Cell Biology 2019Quote: ... The proteins were digested in less than 4 M urea by endoproteinase LysC (Wako Chemicals) followed by digestion with Trypsin Gold (Promega) in less than 2 M urea ...
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bioRxiv - Cancer Biology 2020Quote: Selected protein spots were excised from the gel and enzymatically digested in-gel essentially as previously described(4) using trypsin (Promega). Gel pieces were washed with 50% acetonitrile to remove SDS ...
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bioRxiv - Cancer Biology 2021Quote: ... assessed every day for 4 days by measuring ATP content through Cell Titer-Glo® Luminescent Cell Viability assay (Promega), was compared to cell viability assayed at day 1 ...
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bioRxiv - Immunology 2020Quote: ... Single CD3-CD8-CD16- CD20+Ova-RBD-PE+RBD-AF647+ B cells were sorted into individual wells of a 96-well plates containing 4 μl of lysis buffer (0.5 X PBS, 10mM DTT, 3000 units/mL RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III (Becton Dickinson) ...
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bioRxiv - Cell Biology 2022Quote: ... peptides were diluted 4-fold with ABC buffer (Ammonium bicarbonate 50 mM pH8) and digested with Trypsin MS grade (Promega) overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligos were then ligated at 4°C overnight to the appropriate digested ACR-16-REM sequence (T4 ligase, Promega, USA) and transformed into DH5α (ThermoScientific ...
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bioRxiv - Cell Biology 2023Quote: ... For in vitro import assay WT and MLS-TFEB cDNA were cloned into the pGEM-4 vector and transcribed in vitro using the TNT Quick coupled translation system (L1170, Promega), by incubating 200 ng of DNA with 7 μL of TNT-T7 reticulocyte lysate mix and 0.5 µL of 35S-labelled methionine (NEG709A500UC ...
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bioRxiv - Cell Biology 2023Quote: ... S2R+ cells were grown in SF-4 medium and cotransfected with desired plasmids using FuGENE® HD transfection reagent (Promega). Specifically ...
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bioRxiv - Biochemistry 2023Quote: ... Selected gel bands that did not bind to the 4’-methylgenistein beads were subjected to in-gel digestion with trypsin (MS grade; Promega). Peptide fragments were analyzed using a Q-TOF/MS ...
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bioRxiv - Microbiology 2023Quote: ... In vitro mRNA synthesis was then carried out at 37 °C for 4 h in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... Target and effector cells were cultured 4 hours or overnight and cytotoxicity was measured using the CytoTox96 LDH assay (Promega) or using click beetle green luciferase expressing cells and read on a BioTek Synergy H1 plate reader (BioTek Instruments) ...
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bioRxiv - Genetics 2023Quote: ... carrying the non-risk or risk allele of each SNP (Supplemental Table 4) were cloned into the no-promoter firefly luciferase reporter plasmid pGL4.14 [luc2/Hygro] (Promega, #E6691) or minimal promoter firefly luciferase reporter plasmid pGL4.26 [luc2/miniP/Hygro] (Promega ...
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bioRxiv - Microbiology 2024Quote: ... In vitro mRNA synthesis was then carried out at 37 °C for 4 h in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... The solution was then diluted to 4 mL with 50 mM ammonium bicarbonate and subjected to overnight digestion at 37°C with trypsin (Promega) using an enzyme to substrate ratio of 1:200 (w/w) ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were digested for 6 h at 37°C with 4ng.μL−1 of modified trypsin (Promega) dissolved in 50 mM NH4CO3 ...
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bioRxiv - Genetics 2021Quote: Kidney pieces (1/3 of kidney) were homogenized in Passive Lysis Buffer (Promega) containing protease inhibitors (Thermofisher) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and ligated in a 1:3 vector:insert molar ratio using T4 ligase (Promega). Ligations were transformed into JM109 cells ...
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bioRxiv - Developmental Biology 2023Quote: ... and rabbit anti-Caspase-3 polyclonal antibody (1:250, Promega/Fisher, PR-G7481) overnight at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... Liposomes containing phosphatidylserine and PIP2 (3:1 ratio mol/mol) were purchased (Promega) and prepared to a final concentration of PIP2 at 25 μM ...
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bioRxiv - Microbiology 2023Quote: ... 5’-CGCCTTCGTTACAAGCATCG-3’; antisense, 5’-AAGAGCAAACGCATCTCCGA-3’), GAPDH (sense, 5’-ACCCACTCCTCCACCTTTGAC-3’; antisense, 5’-CTGTTGCTGTAGCCAAATTCGT-3’) using Green GoTaq master mix (Promega, Madison, WI) with C1000 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... samples were digested with 1mg/ml of RNAseA for 1h at 37°C followed by 15 min inactivation at 72°C and addition of RNAseIN (Promega), or with DNase using the Turbo DNA-free kit (Ambion ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of repair template was transfected into 200,000 cells using a 3:1 ratio Fugene6:DNA (Promega), after overnight transfection cells were grown in fresh medium for 6 hours ...
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bioRxiv - Microbiology 2021Quote: ... D-luciferin potassium salt (Promega) stock solution was prepared in sterile PBS at 33.33 mg/mL ...
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bioRxiv - Microbiology 2023Quote: ... D-luciferin potassium salt (Promega) stock solution was prepared in sterile PBS at 33.33 mg/mL and stored in a -20°C freezer ...
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bioRxiv - Molecular Biology 2021Quote: ... were inserted using XhoI and NotI sites in 3’ UTR of Renilla gene in the psiCheck-2 dual luciferase reporter vector (Promega). The reporter in the amount of 25 ng and plasmids with different NORAD variants in the amount of 200 ng were introduced per 30,000 cells in 24-well plates ...
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bioRxiv - Synthetic Biology 2019Quote: The wild type and mutant 3’ UTR of BRCA1 were constructed into psi-check-2 vector (Promega, Madison, WI, USA). Cells were plated in 24-well plates and the plasmids (psi-check-2-WT ...
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bioRxiv - Molecular Biology 2023Quote: ... or PCR amplified from the genome (Tom-3’UTR, primers are in Table S1) and cloned into the psiCHECK-2 vector (Promega) linearized with XhoI and NotI restriction enzymes ...
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bioRxiv - Cell Biology 2023Quote: ... Intracellular fluorescence was monitored after 2 and 3 h of nanoalgosome incubation by spectrofluorimetric analysis using a microplate reader (Glomax, Promega). As vehicle-control ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM CaCl 2 and 10 ng/μL trypsin (Promega). Trypsin digestion was performed at 37 °C with rotation overnight and the beads were washed three times with PBS and distilled water again ...
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bioRxiv - Plant Biology 2019Quote: ... regions of 150 bp to 300 bp were amplified from the plasmids using gene specific primers (Supplementary Table 4) and the amplicons were labeled by random priming (Prime-a-Gene® Labeling System, Promega). U6 was monitored as an equal loading control.
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bioRxiv - Genetics 2022Quote: ... was transiently transfected with the pRL-TK Renilla internal control vector (50 ng) using 4 μL Viafect™ transfection reagent (Promega) into suspension cell lines or adherent cell lines ...
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bioRxiv - Cancer Biology 2019Quote: ... The cells were incubated for 4 days and cell viabilty was measured using CellTiter-Glo® Luminescent Cell Viability Assay (Promega).
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bioRxiv - Systems Biology 2019Quote: ... lysates were diluted in 700µl of TBS and incubated at 4°C with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs) ...
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bioRxiv - Cell Biology 2019Quote: ... Bound fractions were then washed with 1xPBS to remove excess salt followed by RQ1 DNase treatment at 37° C for 10 minutes [4 units of RQ1 (Promega M6101) and 60 units of SUPERaseIN (ThermoFisher AM2694)] ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were diluted 10X in 50 mM ammonium bicarbonate after which 4 µg of sequencing-grade trypsin (Promega, Madison, WI, USA) was added before overnight incubation at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... infected target cells were washed with DPBS and the medium was replaced with 50 μl of RPMI 1640 with 4% low IgG serum (Promega # G711A) containing 5 x 104 Jurkat effector cells (Promega # G701A ...
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bioRxiv - Biochemistry 2022Quote: ... Cells continued to be scanned every 8 h for a further 4 days before being assessed for the number of viable cells using Cell Titer Blue® (Promega) according to the manufacturers instructions ...
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bioRxiv - Microbiology 2020Quote: ... transfection experiments) or 4×105 cells (12 well format; IFN experiments) were lysed in 120 μl passive lysis buffer (Promega #E1941). Half the lysate was used to quantify luciferase protein (Promega Luciferase Assay System #E1501 ...
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bioRxiv - Cell Biology 2021Quote: ... for 4 hours at 37°C and boosted with an extra 2µl Trypsin/LysC (Promega V5073: 20ug + 80uL 50mM acetic acid) overnight at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... 96 and 120 h after transfection and 4 h after adding 20 µl of CellTiter 96® AQ One Solution Reagent (Promega) to each well containing transfected TCam-2 cells.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were incubated for 4 days and cell viabilty was measured using CellTiter-Glo® Luminescent Cell Viability Assay (Promega).
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bioRxiv - Immunology 2021Quote: The PCR product of the targeted region within exon 4 of the TRDC gene was subcloned into the pGEM-T Easy Vector system (Promega, Germany) in accordance with the manufacturer’s protocol and then transformed into XL10 bacteria ...
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bioRxiv - Immunology 2020Quote: ... Single CD19+IgG+RBD+ cells were sorted into a 96 well plate containing 4 µL lysis buffer (12 units of RNasin Ribonuclease Inhibitor (Promega, N2511), 10 mM DTT and PBS x0.5 ...
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bioRxiv - Cell Biology 2021Quote: ... digoxigenin-labeled probes were synthesized from the full-length medaka cDNA of oct-4 (stem cell marker also known as pou5f1; [38]) using pGEM®-T Vector (Promega) linearized plasmid ...