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Citations for Promega :
351 - 400 of 4905 citations for 6 4 METHOXY PHENYL 3 METHYL IMIDAZO 2 1 B THIAZOLE 5 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... 8μL of Fugene-6 (Promega, E2691) was mixed with various amounts of plasmid DNA and allowed to incubate at room temperature for 15 minutes before addition to cells ...
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bioRxiv - Cell Biology 2021Quote: ... FuGENE® 6 Transfection Reagent (Promega); Luminata Forte Western HRP substrate (Millipore) ...
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bioRxiv - Microbiology 2019Quote: ... using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... MDCK) or with FuGENE 6 (ProMega) (HeLa) ...
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bioRxiv - Cancer Biology 2021Quote: ... using FuGENE 6 Transfection Reagent (Promega) according to manufacturer protocol ...
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bioRxiv - Genomics 2023Quote: ... and transfected (Fugene 6, Promega # E269A) with pBABE-puro SV40 LT plasmid (Addgene # 13970 ...
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bioRxiv - Immunology 2023Quote: ... using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl 5× Buffer (Promega), 2 μl MgCl2 (25mM) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 1 µg DNA and 3 µl FuGENE HD (Promega, E2311) in 150 µl Opti-MEM media according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... These cells were transfected in 70% confluent 6-wells TC-treated plates with 2 µg TGFβ receptor-Nanoluciferase constructs (constructs were provided by Promega and Promega R&D) using a 1 µg DNA ...
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bioRxiv - Biochemistry 2022Quote: The 4330bp PCR product amplified from M13mp18 by using primers: oM13-5-27 and oM13-3-24 was inserted into pGEM®-T Easy(PROMEGA #A137A). The resultant plasmid is named as pM13 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RBD-R: 5′-TGC CTC GAG CTC AAG TGT CTG TGGATC AC-3′) into pGEM T-easy vector (Promega, Madison, WI, USA). A standard curve was generated by determining the copy numbers derived from serial dilutions of the plasmid (103–109 copies) ...
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bioRxiv - Biochemistry 2024Quote: ... GSK-3 (Promega), CK1 (Promega) ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM EGTA, 5% glycerol) and freshly supplemented reagents (1 mM DTT, 1 mM ATP, 1 mM PMSF, protease inhibitor mix (Promega) and 1% Tween 20) ...
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bioRxiv - Microbiology 2022Quote: ... 1 complete protease inhibitor tablet EDTA-free and 5 µl DNaseI (Promega), lysed using a cell disruptor and the insoluble fraction removed by centrifugation (15,000 xg ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μl of 5 ng μl−1 trypsin (sequencing grade, Promega®) in 50 mM AHC plus 1 mM CaCl2 were added to each well and incubated for 20 min at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 4 h later 5 µl/well of NanoBRET™ Nano-GloR Substrate (10 µl/ml in DMEM no phenol red, Promega) was added and 460 nm donor and 618 nm acceptor signals were read within 10 min of substrate addition using CLARIOstar microplate reader (Mandel) ...
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bioRxiv - Cell Biology 2020Quote: ... One 10-cm plate of HEK-293T cells at 90% confluency was transfected with 6.5 μg of the pBMN-ARHGAP36 (isoform 2)-mCherry mutant library and 4 μg of pCL-ECO using the FuGene HD transfection reagent (Promega). The medium was replaced after 24 hours with DMEM containing 1.8 mM L-glutamine ...
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bioRxiv - Biophysics 2020Quote: ... Plasmids were transfected in cells (passage number < 35) seeded in 96-well plates at ~50 % confluence 2-4 days before the experiment with FuGene6 (Promega) or Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... Plasmids were transfected in cells (passage number < 35) seeded in 96-well plates at ~50 % confluence 2-4 days before the experiment with FuGene6 (Promega) or Lipofectamine 2000/3000 following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was then digested with 2.5 μg Lys-C (Wako, Japan) for 4 h at 37°C and 2 μg trypsin (Promega, V5113) for an additional 4 h at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Nano-Glo Luciferase Assay Substrate furimazine (2 µl ml-1, Promega) was added and the nLuc released upon proteolytic cleavage of the receptor was measured on a plate reader Mithras LB 940 (Berthold Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... before cells were permeabilized using 0.2% Triton X-100 PBS for 10 min at room temperature, then blocked for 30 min (2% BSA / 0.5% fish skin gelatine / PBST.5, 2U/µL RNAsin Plus (Promega) at room temperature) ...
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bioRxiv - Biochemistry 2020Quote: ... the samples were brought to room temperature for 5-10 minutes while 2 mL of 30 mM coelenterazine (Promega), or 15 mL of 1-to-50 diluted NanoLuc substrate (Promega) ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were trypsinised on the beads in 60 μl of Buffer I (2 M urea, 50 mM Tris-HCl pH 7.5, 5 μg/ml Trypsin [modified sequencing-grade trypsin; Promega]) for 30 min at 37°C in a thermomixer ...
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bioRxiv - Biochemistry 2019Quote: ... All samples were further diluted with 20 mM HEPES pH 8.0 to a final urea concentration of 2 M and proteins were digested with 5 μg trypsin (V5111, Promega) (1/100 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were further diluted with 50 mM Tris pH 7.9 pH 8.0 to a final urea concentration of 2 M and proteins were digested with 5 µg trypsin (Promega) (1/100 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were diluted using one volume of dilution buffer (10 mM Tris pH 8, 0.4% NP40, 5 mM CaCl2, 2 U/mL RQ1 DNAse (Promega)) and then incubated with anti-Flag or anti-p400 antibodies coupled to agarose beads (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... pCAG-DCC:TDTOMATO and pCAG-DCCkanga:TDTOMATO plasmids (1 µg) were transfected into the plated U251 cells using FuGENE 6 (Promega) in Opti-MEM (Gibco ...
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bioRxiv - Immunology 2020Quote: ... were transfected with 4.5 µg of the pBabe-puro-NCAM140 mApple construct (NCBI reference sequence: NM_000615.6, transcript variant 1) using Fugene 6 Transfection Reagent (Promega). Supernatant containing viral particles was collected and concentrated using PEG-IT (System Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were digested overnight at 37 °C with 6 ng μL-1 of Asp-N (Promega, UK). Peptides were extracted in 2 % v/v formic acid ...
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bioRxiv - Developmental Biology 2022Quote: ... were transfected into NCCIT cells (ATCC) at ∼70% confluency using a 6:1 ratio of Fugene HD (Promega) for 48 hours in ATCC-formulated RPMI media ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested off the beads by adding 6 μL of 1 μg/μL sequencing grade Trypsin (Promega), diluted in 50 mM acetic acid ...
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bioRxiv - Molecular Biology 2020Quote: ... and proteins were sequentially digested with LysC (1:50, w/w; FUJIFILM Wako) for 3 h at 37 °C and trypsin (50:1, w/w; Promega) overnight at 37 °C ...
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bioRxiv - Genetics 2019Quote: Buccal cell samples were collected and prepared as for the standard processing method with the exception that 3 μl 5 x AmpSolution™ (Promega Corporation, USA), was added to the PCR reagents in place of low TE buffer ...
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bioRxiv - Immunology 2021Quote: ... CDC was measured after incubation for 3 hours at 37 °C 5% CO2 with a luminometer using the CytoTox-Glo Cytotoxicity Assay (Promega; Cat. Nr.: G9291) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2023Quote: ... The amplicons generated from the 5’ and 3’ race PCRs were subjected to purification and subsequent cloning into the pGEM-T easy vector (Promega Corp., WI, USA). The constructed vectors were sequenced (Plasmidsaurus ...
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bioRxiv - Cell Biology 2021Quote: ... Donor 293T cells were co-transfected with 1.5 µg of plasmids encoding different untagged S mutants and 0.6 µg of pmCherry-N1 using 6 µL of Fugene 6 following the manufacturer’s instructions (Promega). Acceptor Vero cells expressing human ACE246 ...
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bioRxiv - Microbiology 2020Quote: ... Donor cells were co-transfected with 1.5 μg pCAGGS-S and 0,5 μg pmCherry-N1 using 6 μl of Fugene 6 following the manufacturer’s instructions (Promega). Acceptor cells were treated with CellTracker™ Green CMFDA (5-chloromethylfluorescein diacetate ...
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bioRxiv - Cell Biology 2020Quote: ... Then samples were diluted 3 times in the buffer and 1 µg trypsin (Gold. Promega) was added overnight at 30°C ...
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bioRxiv - Neuroscience 2020Quote: MAO B catalytic activity was determined by MAO-Glo Assay (Promega). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... were stained with CaspACE FITC-VAD-FMK (fluorescein isothiocyanate-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone) in situ Marker (Promega, Mannheim, Germany) as per manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: The effect of DMSO and methyl ester version compounds (compound 34–38) on cell viability was determined using a CellTiter-GloTM ATP detection system (Promega #7573). For testing DMSO tolerance ...
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bioRxiv - Genomics 2019Quote: ... sodium bisulfite conversion reagents were used to treat 500 ng of each DNA pool (Methyl EdgeTM Bisulfite Conversion System, Promega, USA). The DMRs for validation were randomly selected from the bioinformatics analysis results ...
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bioRxiv - Cell Biology 2020Quote: ... with Fugene 6 transfection reagent (Promega E2691). 10 mM sodium butyrate was then added to the transfected cells 3 hours post transfection for an overnight incubation at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfection was performed with Fugene 6 (Promega). Supernatant was filtered at 0.45 µm before storage at −80°C For stable expression of TRIM21 ...
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bioRxiv - Molecular Biology 2021Quote: ... and pCIG2 UBE3AT485A using Fugene 6 (Promega). 4 hours post transfection cells were treated with chemical libraries ...
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bioRxiv - Cell Biology 2019Quote: ... polyethylenimine “MAX” (polysciences) or Fugene 6 (Promega) were used ...
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bioRxiv - Cancer Biology 2019Quote: ... FuGENE 6 (Promega, E2691, Madison, WI, USA) was used ...
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bioRxiv - Molecular Biology 2022Quote: ... or FuGENE® 6 (Promega Cat# E2961) as transfection reagents ...