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Citations for Promega :
551 - 600 of 741 citations for 6 β NALOXOL HCL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: HEK293T cells were transfected with either Tau-encoding or control constructs using Fugene 6 (Promega, cat. no. E2691) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was assessed 6 days post-treatment using a Cell-TiterGlo 3D Cell Viability assay (Promega, G9683), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with LDLR-GFP (WT, C75W, and C75W/C34W) for 48 h with Fugene 6 (Promega). Cells were starved in 5% LPDS media (Millipore Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with 8 µg of pcDNA3.1-GFP-mNLS-K145Q using Fugene 6 Transfection reagent (Promega, E2692) per manufacturer protocol ...
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bioRxiv - Microbiology 2024Quote: ... Cells were transfected with 500 ng of each plasmid using 0.4 µl of Fugene 6 according to the manufacturer’s instructions (Promega) for 40 hours ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Comparison of VUF15485-induced β-arrestin2 recruitment to human and mouse ACKR3 was also measured by NanoLuc complementation assay (NanoBiT, Promega Corporation, Madison, WI, USA) in HEK293T cells (Dixon et al. ...
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bioRxiv - Immunology 2022Quote: Replication competent HIV were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... 3 ug of pCAG-NLS-Bxb1 was diluted in 250 uL of OptiMEM and 6 uL of Fugene (Promega). On day 2 ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated for an additional 6 hours at 37°C before 75μl of Bio-Glo Luciferase Assay Reagent (Promega) was added to each well ...
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bioRxiv - Molecular Biology 2021Quote: ... All the clones were transfected into the HEK-293T cells at 90% confluency using FuGene 6 as transfection reagent according to the manufacturer’s instructions (Promega). dsiRNAs targeting Rpp30 (10 nM ...
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bioRxiv - Immunology 2019Quote: ... 0.4 μg envelope vector (pMD2.G) and 1.6 μg hairpin-pLKO.1 vector (SHC016 control or gene specific shRNA. Fugene-6 (Promega) was used as transfection reagent ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μg of sgRNA construct and 1.5 μg of linearized HDR plasmid were transfected into RPE1 cells using Fugene 6 (Promega). Positive cells were FACS sorted to isolate the Ndc80-EGFP expressing cells and single clones were identified by visual inspection with fluorescent microscope ...
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bioRxiv - Biochemistry 2019Quote: ... 70 000-100 000 cells (in 200-450 μl DMEM) were seeded into each well containing a transfection cocktail of 0.4 μl of Fugene 6 (E2693, Promega), with 20 μl Opti-MEM (11058-021 ...
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bioRxiv - Developmental Biology 2019Quote: ... were injected with dsRNA throughout and frozen in Trizol on dry ice after 6 days in vitro for RNA extraction according to manufacturer’s protocol and DNAse (Promega) treatment for 30 min at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... were transfected with 1 µg of DNA and 4 µL FuGENE 6 in 100 µL Opti-MEM media for 24-36 hours according to the manufacturer’s instructions (Promega). Cells were fixed with 4% (v/v ...
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bioRxiv - Immunology 2021Quote: ... The antibodies were purified from the culture supernatant 4 – 6 days later using protein G magnetic beads (Promega #G7472). Purified antibodies and antibody elution buffer (5 parts elution buffer (100 µM glycine-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... RPE1 cells were co-transfected with 1 µg KIF1C-mCherry-FRB and 0.5 µg FKBP-GFP-Myc-MAO using Fugene 6 (Promega) in fibronectin-coated glass-bottom dishes and imaged 24 hours later on an Olympus Deltavision microscope (Applied Precision ...
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bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 spike protein (23) into HEK 293T cells using the FuGENE 6 Transfection Reagent (Promega). Spike sequences from the following global strains REF were used ...
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bioRxiv - Molecular Biology 2022Quote: ... Fresh bacmid DNA was transfected into Sf9 cells at 0.5×106 cells/mL in 6-well cell culture plates using FuGene HD (Promega) according to the manufacturer’s protocol (final concentration 10 μg/mL) ...
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bioRxiv - Immunology 2021Quote: ... SIV pseudovirus Env construct was cotransfected with env-deficient backbone plasmid (pSG3ΔEnv) in a 1:2 ratio with transfection reagent FuGENE 6 (Promega) in HEK 293T cells according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells were transfected with WT or mutant C10 constructs using the FuGENE 6 transfection reagent (Promega, cat# PRE2691). The cells were incubated at 37 C at least overnight before downstream analysis.
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bioRxiv - Neuroscience 2020Quote: ... and 0.4 pmol/well of hairpin constructs (pscAAV-GFP-shFoxP1 or shCtrl) using FuGENE 6 Transfection Reagent (#E2691, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells (1.2 x 106 cells/well, 6-well format) after 24 hpi using passive lysis buffer (Promega) based on the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: HEK293T cells were simultaneously transfected with 1 μg pCS2Cachd1-eGFP or pCS2Cachd1_V1122D-eGFP and 1 μg KDEL-tRFP plasmid using Fugene 6 transfection reagent (Promega). eGFP/tRFP expression was confirmed 24 hours post transfection and the cells fixed at 42 hours post transfection and imaged on a spinning disk microscope (see below).
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with 2 μg of FLAG-tagged β2 or β1 adrenergic receptor DNA using Fugene 6 (Promega) and seeded at a density of ∼70,000 cells.ml−1 on 18 mm glass coverslips 4-8 hours after transfection.
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bioRxiv - Cancer Biology 2020Quote: Viral particles were packaged in HEK293T cells seeded at a density of 1.5×106/10cm petri dishes in a proportion of 24μL Fugene 6 (Promega, E2691) in 136μL serum-reduced OPTI-MEM (Gibco ...
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bioRxiv - Microbiology 2020Quote: Env-pseudotyped luciferase reporter viruses were generated by co-transfection of 810 ng of an env expression vector and 810 ng of pZM247Fv2Δenv backbone (22) using 293T cells and the Fugene 6 reagent and protocol (Promega). Five hours after transfection ...
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bioRxiv - Immunology 2019Quote: ... 0.4 µg envelope vector (pMD2.G) and 1.6 µg hairpin-pLKO.1 vector (SHC016 control or gene specific shRNA. Fugene-6 (Promega) was used as transfection reagent ...
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bioRxiv - Genetics 2019Quote: ... genes were isolated from C57BL/6 mouse genomic DNA by standard PCR and inserted into the pGL4.10 vector (Promega).
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bioRxiv - Neuroscience 2021Quote: ... The constructs (1.2 µg) were cotransfected with vesicular stomatitis virus G (600 ng) and pSPAX2 (800 ng) using FuGENE 6 (Promega). The lentiviral supernatants were collected 2 days after transfection ...
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bioRxiv - Developmental Biology 2020Quote: ... C2C12 myoblasts were seeded at 20-30% confluence in 10cm format (CELLSTAR) and forward-transfection with Fugene-6 (Promega) according to manufacturer’s protocol with the following plasmids ...
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bioRxiv - Biochemistry 2022Quote: ... the D10ACas9 nickase and the HDR template on 6-well plates at 70% confluency using FuGene® HD (Promega). Cells were allowed to recover for 7-10 days (splitting if necessary ...
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bioRxiv - Biochemistry 2022Quote: ... and penicillin (100 U/ml)/ streptomycin (100 μg/ml) (complete media) then transfected with Fugene 6 (Promega, Madison, WI). Cells were then lysed 2 days post-transfection using a lysis buffer containing 150 mM NaCl ...
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bioRxiv - Developmental Biology 2022Quote: ... into a 10 cm plate of HEK293T cells at 60-70% using Fugene 6 transfection reagent (Promega, Cat #E2691). Media containing lentiviral particles were collected at 24 ...
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bioRxiv - Cell Biology 2022Quote: ... pX459-derived plasmids encoding both Cas9 and the gRNA were transfected using Fugene 6 (cat. no. E2692 from Promega). 24 h post-transfection puromycin was used to select for transfected cells ...
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bioRxiv - Cell Biology 2022Quote: ... were transfected with 2 μg of fresh bacmid DNA and 6 μL of FuGene HD transfection reagent (Promega, E2311) according to the manufacture’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... fresh bacmid DNA was transfected into Sf9 cells at 0.5×106 cells/mL in 6-well cell culture plates using FuGene HD (Promega) according to the manufacturer’s protocol (final concentration 10 μg/mL) ...
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bioRxiv - Cell Biology 2023Quote: ... and 500 ng of lenti-viral transfer plasmids previously described along with 3 µl of Fugene 6 (E2692, Promega) transfection reagent ...
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bioRxiv - Neuroscience 2023Quote: ... We amplified the regions from C57Bl/6 tail snip DNA or from human male genomic DNA (Promega catalog # G1471) using FastPhusion 2x Master Mix (Thermo Fisher catalog # F548L) ...
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bioRxiv - Microbiology 2023Quote: ... 6 μg of IMC was transfected into 293T cells in a T25 flask using the FuGENE6 transfection reagent (Promega). The cells were cultured at 37°C for 6 hours before the medium was replaced by fresh medium ...
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bioRxiv - Bioengineering 2023Quote: ... PB-CAG-H2B-mCherry-IPuroR and pCMV-hyPBase were co-transfected into KhES-1 using Fugene 6 (Promega, E269A) transfection reagent following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: HFF or HEK293 cells (3 × 104 cells per well of a 24-well plate or 1 × 105 cells per well of a 6-well plate) were reverse transfected with 0.5 μg of pCW57-CMV-KDEL-mCherry-GF using Fugene HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Beads were washed 6 times with 50 mM ammonium bicarbonate and then treated with TPCK-treated modified trypsin (Promega) for 16 hours at 37°C on an end-over-end rotator ...
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bioRxiv - Biochemistry 2023Quote: BromoTag cell lines were generated in HEK293 cells via simultaneous transfection of two vectors at a 4:1 reagent:DNA ratio with FuGENE 6 (Promega). The first vector was a pMK-RQ vector containing 500 bp homology arms on either side of either an eGFP-IRES-BromoTag or eGFP-IRES-HiBiT-BromoTag sequence for integration into MCM4 and BRD4 ...
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bioRxiv - Neuroscience 2024Quote: ... were transfected 2 days after plating with 1 μg of total DNA with FuGENE 6 transfection reagent (E2691; Promega) at a ratio 1:3.
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Loss of intermicrovillar adhesion impairs basolateral junctional complexes in transporting epitheliabioRxiv - Cell Biology 2024Quote: A validated CDHR2 KO CL4 cell clonal population was transfected with pHALO-N3-CDHR2 using FuGENE 6 (Promega #E2691) at a FuGENE:DNA (μL:μg ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293TN cells were seeded in 6-well plates and transiently transfected with plasmid of choice using FuGene transfection reagent (Promega) and OptiMem (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... S2-CP8 cells were then transiently transfected with both mRFP-FKBP-5-ptase-dom and PM-FRB-CFP (0.5 μg/well of a 6-well plate for each vector) with ViaFect (Promega Corp.). After 24 h culture ...
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bioRxiv - Cell Biology 2019Quote: Glucose uptake was measured based on the detection of 2-deoxyglucose-6-phosphate uptake by a commercially available luminescence-based kit (Glucose Uptake-GloTM Assay, Promega) on a SpectraMax M3 (Molecular Devices) ...
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bioRxiv - Neuroscience 2021Quote: ... coated 12-well plate and transfected the next day using the Fugene 6 transfection reagent and according to the manufacturer’s directions (Promega E2691). The same amount of plasmid was transfected per condition ...