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Citations for Promega :
401 - 450 of 986 citations for 5 Pyrimidinecarbonitrile 4 hydrazino 8CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... and GST- GBF3 were purified from 4 ml culture and immobilized on MagneGST Glutathione particles (Promega). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... then transfected with 4 μg pLZRS-GCaMP6 DNA plus 12 μl FuGENE 6 (Promega Cat. #E2691) in 800 μl of Opti-MEM (Thermo-Fisher Cat ...
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bioRxiv - Microbiology 2023Quote: ... then digested with 4 μL of a trypsin/Lys-C Mix (0.5 μg/uL) (Promega # V5071) using a two-step in-solution digestion overnight at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... or MEK inhibitor U0126 (1, 10, and 50 µM for 4 h; Promega, Madison, WI, USA). A corresponding volume of dimethyl sulfoxide (Nacalai Tesque ...
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bioRxiv - Biochemistry 2023Quote: ... HRP (4 μM) was added followed by 20 μL of Nano-glo luciferase substrate (Promega, N1110) to each well ...
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bioRxiv - Genetics 2021Quote: ... then a 5 min final extension (72 °C)—using GoTaq Green Master Mix (M7123, Promega) and the pCFD6_seqfwd and pCFD6_seqrev primers (Table S1 ...
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bioRxiv - Molecular Biology 2020Quote: Cells were blocked by pipetting 50ul of 5% Bovine serum albumin (BSA) (Promega, Cat: W3841) directly onto slides ...
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bioRxiv - Immunology 2019Quote: ... RAC2 3’UTR-: 5’-TTGCGGCCAGCGGCCGCTCTCCAAACTTGAATCAATAAATTT-3’ and inserted into a dual luciferase psiCHECK2 backbone (Promega). RAC2 mutant 3’UTR constructs were generated using Infusion HD cloning kit (Clontech ...
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bioRxiv - Genetics 2021Quote: ... DNA ligation was performed using 5 μl HC T4 DNA ligase (Promega, 50-100 U) in 1X Ligation Buffer (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... the 5’-end of each mRNA was capped using the Ribo m7G Cap Analog (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... alkylated with iodoacetamid (final concentration of 5 mM) and digested overnight with trypsin (Promega V5113) in 1 M urea at an enzyme-to-substrate ratio of 1:40 ...
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bioRxiv - Plant Biology 2021Quote: ... each primer at 0.25 μM and 5 μL of 2X GoTaq qPCR Master Mix (Promega). The amplification program consisted of preincubation steps at 50°C for 2 min and 95°C for 10 min followed by 40 cycles of 95°C for 15 s and 60°C for 1 min ...
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bioRxiv - Microbiology 2021Quote: ... Samples were digested overnight at 37 °C with 5 µg sequencing-grade modified trypsin (Promega). The samples were then acidified with trifluoroacetic acid (TFA ...
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bioRxiv - Microbiology 2020Quote: ... Cells (5 × 106) were then lysed using 100 μl of RIPA lysis buffer (Promega, WI) supplemented with 1 μl of proteinase inhibitor and 10 μl of phenylmethylsulfonylfluoride ...
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bioRxiv - Molecular Biology 2022Quote: ... terminated by incubation on ice and diluted 1:5 in Glo Lysis Buffer (Promega # E2661). 25 μL of prepared Nano-Glo reagent (Promega # N1120 ...
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bioRxiv - Systems Biology 2019Quote: ... Half of the cDNA (5 μl) was amplified by PCR using Pfu Polymerase (Promega, M7745) with the cycling conditions (95°C for 2 min ...
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bioRxiv - Cancer Biology 2019Quote: ... before dilution with 120 μL 0.04 × ProteaseMax and 5 μg/μL sequencing grade trypsin (Promega) in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Survival was determined after 5 days of incubation using the Cell Titre-Blue assay (Promega). All cell lines were treated with 4 and 8 µM Erastin and 3 and 6 µM RSL3 ...
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bioRxiv - Plant Biology 2022Quote: ... DNA was incubated at 80°C for 5 min and mixed with FuGENE HD (Promega) in a ratio of 3:1 (FuGENE:DNA ...
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bioRxiv - Plant Biology 2022Quote: ... and 0.3 µl of Taq polymerase (5 u/µl) provided by Promega (Madison, Wisconsin, U.S.A.). Variations in MgCl2 (0.5–1 µl ...
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bioRxiv - Plant Biology 2023Quote: ... 5’RACE products were gel-purified and cloned in pGem®-T Easy vector (Promega). Independent clones were randomly chosen and sequenced by Sanger sequencing method.
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bioRxiv - Cancer Biology 2023Quote: ... resuspended in 400 μL 50 mM NH4HCO3 containing 5 μg modified sequencing grade trypsin (Promega), then transferred to a protein LoBind tube (Eppendorf ...
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bioRxiv - Biochemistry 2023Quote: ... with 25 μL of diluted reactions (diluted 1:5 in Glo Lysis Buffer; Promega # E2661) and incubated at room temperature for 5 min ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μg of the isolated recombinant bacmid and 5 μL Fugene HD transfection reagent (Promega) were diluted in 150 μL Sf-900™ III SFM ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... cDNA synthesis from 5 µg RNA was performed using the GoScriptTM Reverse Transcriptase Kit (Promega). Next ...
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bioRxiv - Cell Biology 2023Quote: ... Purified 5 μg of pAd/CMV/V5-DEST plasmid was transfected using Fugene HD (Promega) when the 293A cells were 80% confluent ...
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bioRxiv - Cancer Biology 2024Quote: ... After 5 days cells were collected per manufacturer’s instructions using either CellTiterGlo® (Promega, G7570) to measure viability by assessing ATP concentrations ...
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bioRxiv - Biochemistry 2024Quote: ... Luciferase activity was measured 5 h later using Bright-Glo Luciferase Assay System (Promega, E2620) on a Tristar 5 microplate reader (Berthold).
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bioRxiv - Molecular Biology 2024Quote: ... 5–10 embryos were collected at 3 hpf and lysed in Passive Lysis Buffer (Promega) containing cOmplete Protease Inhibitor Cocktail (Sigma‒Aldrich) ...
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bioRxiv - Synthetic Biology 2019Quote: ... ligated for 3 h at room temperature (RT) or overnight at 4°C using T4 ligase (Promega), and propagated in E.coli XL 10 Gold cells (Agilent) ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were diluted 4-fold prior to overnight digestion at 37 °C with trypsin (Trypsin Gold, Promega) at an enzyme to substrate ratio of 1:50 ...
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bioRxiv - Cell Biology 2022Quote: ... 4 μl of each reaction was spotted onto a streptavidin-coated SAM2 Biotin Capture Membrane (Promega, #TB547). The membrane was air-dried ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were diluted 4-fold with 100 mM ammonium acetate and digested with sequence-grade trypsin (Promega) overnight at RT at an enzyme:substrate ratio of 50:1 (w/w) ...
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bioRxiv - Cancer Biology 2022Quote: ATRX knock-out clone were established by transfecting SK-N-AS and GI-ME-N with the Cas9 expressing vector containing ATRX_KO_sgRNA_2 (targeting exon 4) and the corresponding homology arm plasmid using Fugene HD transfection reagent (E2312, Promega). Three days after transfection ...
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bioRxiv - Genetics 2022Quote: ... Samples were diluted 1:4 with 50mM Tris/HCl (pH 8.0) and sequencing grade modified trypsin (Promega) was added in an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Cancer Biology 2023Quote: ... ZR-75-1 cells were short tandem repeat tested every 4 months (Stem Elite ID System, Promega). Doxorubicin (S1208) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured at day 4 and day 6 using the Cell-Titer Glo assay (Promega). Viability data was analyzed by comparing the relative viability change between base editing with the uORF gRNA and the associated parental CDS gRNA ...
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bioRxiv - Biochemistry 2023Quote: ... and assayed with 4 μM of HRP and 20 μL of Nano Glo luciferase substrate (Promega, N1110) diluted to 1:1000.
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bioRxiv - Cell Biology 2020Quote: ... or SL1-deleted SARS-CoV-2 5’ UTR luciferase reporter plasmid using FuGENE Transfection Reagent (Promega). Luciferase assays were performed using the Luciferase Assay System (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: 5–10 oocytes were lysed by vigorous shaking and pipetting in Passive Lysis Buffer (Promega, E1980), using 20 µl per oocyte ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and 4uL 5× GoTaq buffer and 1.25 U GoTaq DNA polymerase (Promega Corporation, Madison, WI, USA) according to the recommended protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNAs were diluted 1:5 and qPCR was performed using GoTaq® qPCR Master Mix (Promega). Primers for mCyb5r3 were ...
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bioRxiv - Pathology 2020Quote: 5 µL GoTaq® Probe qPCR Master Mix with dUTP (2X) (Promega, Cat # A6120 and A6121)
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A key interfacial residue identified with in-cell structure characterization of a class A GPCR dimerbioRxiv - Biochemistry 2019Quote: ... cells were washed twice with PBS and incubated with 5 μM coelenterazine H (Promega, Madison, USA) for 5 min 37 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were co-transfected with 5’ SORL1 proximal promoter fragments in pGL4.10[luc2] vector (Promega, E6651) and pRL-TK Renilla control reporter vector (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions were incubated at 37 °C overnight and subsequently treated with 5 units of DNase (Promega) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... In-gel digestion was performed using 5 ng/μL mass spectrometry-grade trypsin (Trypsin Gold, Promega) in 50 mM NH4HCO3 digestion buffer ...
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bioRxiv - Physiology 2019Quote: ... USA) and digestion with 150 ng trypsin (5 h, 37 °C Promega, Sequencing Grade, Wisconsin, USA) using a Tecan Freedom EVO robotic liquid handler (Tecan Systems ...
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bioRxiv - Microbiology 2019Quote: ... and remaining tissue incubated in 1X HBSS (without Ca2+ and Mg2+) containing 5 mM EDTA (Promega) and 1 mM DL-Dithiothreitol (DTT ...
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bioRxiv - Immunology 2019Quote: ... and the reaction volume was topped up to 5 μL with RNase/DNase-free H2O (Promega). Thermal cycling was carried out using a Viia7 real time PCR system (Applied Biosystems) ...