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Citations for Promega :
1 - 50 of 5379 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... HEK cells were transfected with both heavy and light chain BCR constructs using FuGENE HD transfection reagent (Promega) in a 1:3 ratio ...
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bioRxiv - Immunology 2021Quote: ... Confirmed heavy and light chain plasmid DNA were co-transfected in 293T cells (ATCC) using Fugene transfection reagent (Promega) in 24 well plates for preparing antibody supernatant for initial screening for their expression and antigen specificity as detailed in the following section ...
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bioRxiv - Immunology 2020Quote: ... Gel bands for heavy and light chains were excised and in-gel digested using trypsin (Promega), chymotrypsin (Promega) ...
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bioRxiv - Cell Biology 2019Quote: ... The following primary antibodies against the antigens were used: Tuj-1 (1:300, Promega), Synaptophysin (1:400 ...
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bioRxiv - Microbiology 2020Quote: ... incubating and chromogenic reaction generating were similar to the ACE2 assay instead of that primary antibody binding to antigen was not required and that HRP-conjugated goat anti-human IgG antibody (Promega) would be used as the secondary antibody to detect binding of CB6 antibody to the antigens.
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bioRxiv - Cancer Biology 2019Quote: CHO K-1 cells overexpressing hPD-L1 and the recombinant TCR ligand (hPD-L1 Antigen Presenting Cells, hPD-L1 aAPCs, Promega) and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE ...
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bioRxiv - Plant Biology 2020Quote: Total RNA from light-treated cells was extracted using a Maxwell RSC instrument (Promega) and a Maxwell RSC simplyRNA Tissue Kit (Promega) ...
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bioRxiv - Microbiology 2021Quote: Peptides from cell-surface/biofilm-ECM-associated proteins were released via a Trypsin/Lys-C mix (Promega) at a final concentration of 50 ng µL-1 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Polymerase chain reactions were performed using 2 U Gotaq (Promega), 1X Gotaq buffer (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... and light emission was measured in arbitrary light units on a Glomax 20/20 Luminometer (Promega, Madison WI, USA). The luciferase assays were performed as suggested by the manufacturer’s protocols.
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bioRxiv - Cancer Biology 2022Quote: ... Biotinylated antigens were immobilized using streptavidin-coated magnetic beads (Promega). In each round ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... HaloTag-GST was used as a negative control antigen (Promega).
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bioRxiv - Microbiology 2021Quote: ... The SK-N-SH cell surface proteins with attached TBEV virions were digested off with 1.25 μg trypsin (Promega) and the supernatant collected ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cell surface levels of HiBiT-tagged human S1PR1 were monitored using a Nano Glo HiBiT Extracellular Detection System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Purified EDIII antigens were biotinylated using HaloTag PEG biotin ligand (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Polymerase chain reaction (PCR) was performed using GoTaq kit (Promega, Madison, WI) unless otherwise indicated.
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bioRxiv - Genetics 2021Quote: ... All other polymerase chain reactions (PCR) were performed using the GoTaq (Promega) enzyme system as per manufacturer instructions (95°C for 2 min ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... Polymerase chain reaction (PCR) was performed using GoTaq kit (Promega, Madison, WI).
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bioRxiv - Molecular Biology 2019Quote: ... light emission was detected with a GloMax luminometer (Promega).
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bioRxiv - Cell Biology 2022Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer with Azure Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer with Azure Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... and inserting epitope tags via polymerase chain reaction with Pfu DNA polymerase (Promega). Primers for HA insertion into pIRES2- EGFP-FL ...
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bioRxiv - Zoology 2021Quote: ... Polymerase chain reactions (PCR) were carried out using GoTaq Green Master Mix (Promega) in the following conditions ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative polymerase chain reaction (qPCR) was performed with the GoTaq qPCR Mastermix (Promega) and QuantStudio 5 Real Time PCR (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... Real time-polymerase chain reactions were performed with GoTaq qPCR Master Mix (Promega) according to the manufacturer’s instructions and completed on QuantStudio 6 Flex system (ABI) ...
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bioRxiv - Microbiology 2022Quote: Polymerase chain reaction was performed using the GoTaq PCR master mix (Promega, USA) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Parasites viability was assessed by direct light microscope observations and MTS colorimetric assays (CellTiter Aqueous One Solution Cell Proliferation Assay -Promega); they maintained shape and motility at 5 ...
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bioRxiv - Plant Biology 2021Quote: ... http://cshprotocols.cshlp.org/content/2009/2/pdb.prot5110.abstract) with trypsin (porcine, side chain protected; Promega). Briefly ...
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bioRxiv - Biophysics 2023Quote: ... followed by polymerase chain reaction (PCR) with GoTaq® G2 Master Mix (M7822, Promega) on Biometra TRIO Touch Thermocycler (207072X ...
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bioRxiv - Immunology 2020Quote: ... cells were lysed and luciferase activity (RLU, relative light units) was measured using the Bright-Glo™ Luciferase Assay System (Promega). Background luminescence produced by cells-only controls (no pseudotyped virus ...
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bioRxiv - Immunology 2020Quote: ... ExpiCHO cells then were incubated with Jurkat cells expressing FcγRIIIa receptor or FcγRIIa on their surface and stably transfected with NFAT-driven luciferase gene (Promega, Cat. Nr.: G9798 and G7018) at an effector to target ratio of 6:1 for FcγRIIIa and 5:1 for FcγRIIa ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Relative light units (RLUs) were measured using a luminometer (Promega; GloMax). Triplicate data was analyzed in Prism Graphpad to generate IC50 values.
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bioRxiv - Cell Biology 2024Quote: ... Relative light units (RLUs) were measured using a luminometer (Promega; GloMax). Triplicate data was analyzed in Prism Graphpad to generate IC50 values.
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bioRxiv - Plant Biology 2020Quote: ... Antigen on the membrane was visualized with horseradish peroxidase-coupled anti-rabbit IgG (Promega) for VHA-a1 and VHA-a3 ...
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bioRxiv - Immunology 2021Quote: ... RBD and NTD antigens were site-specifically biotinylated using Halotag PEG biotin ligand (Promega), following the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... Antigen retrieval was performed by incubation with 20 μg/mL proteinase K (Promega, USA) for 20 minutes ...
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bioRxiv - Biophysics 2022Quote: ... The variable and CH1 regions of the NbFab heavy chain were cloned into pTarget (Promega) following a N-terminal (MRPTWAWWLFLVLLLALWAPARG ...
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bioRxiv - Immunology 2023Quote: ... 5% CO2 for 24 hrs, after which luciferase activity (Relative Light Units, RLU) was measured in cell lysates using the Bright-Glo system (Promega, Madison, WI) with a Bio-Tek II plate reader ...
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bioRxiv - Plant Biology 2022Quote: ... Absolute relative light units (RLU) were measured by GloMAX discover system (Promega). Data were analysed as Luciferase/Renilla ratio and compared with the ratio of the leaves infiltrated with A ...
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SARS-CoV-2 accessory proteins ORF3a and ORF7a modulate autophagic flux and Ca2+ homeostasis in yeastbioRxiv - Microbiology 2022Quote: ... The light emission was immediately measured in a GloMax microplate luminometer (Promega) in triplicate and continuously recorded over the indicated time ...
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bioRxiv - Molecular Biology 2023Quote: ... The light emission was continuously recorded on a GloMax microplate luminometer (Promega) in three biological replicas ...
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bioRxiv - Bioengineering 2023Quote: ... selections were performed using biotinylated Fc fusion antigen captured on SA-coated magnetic beads (Promega). Prior to each selection ...
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bioRxiv - Biochemistry 2019Quote: ... a polymerase chain reaction using Pfu DNA polymerase (Thermo Fischer Scientific) or Pfu DNA polymerase (Promega), the template pNZ-SV10HnisT or pIL-SVnisT and the primer pair nisTH551Afor and nisTH551Arev (Supplementary File 4 ...
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bioRxiv - Plant Biology 2020Quote: ... Light emission was measured immediately in a GloMax 96 Microplate Luminometer (Promega, USA). At least 16 leaf disks were taken by treatment (n ≥ 16).
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bioRxiv - Immunology 2022Quote: ... and relative light unit (RLU) values were read on a Glomax® (Promega) or BioTek Synergy™ H1 (Agilent ...
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bioRxiv - Plant Biology 2023Quote: ... Light emission was measured using a luminometer (GloMax® Discover, Promega, United States).
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bioRxiv - Microbiology 2024Quote: ... Relative light units (RLU) were determined using the GloMax 20/20 Luminometer (Promega). GFP intensity was analyzed using a fluorescence microscope (Mshot ...
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bioRxiv - Biophysics 2021Quote: ... All DNA handles were made by polymerase chain reaction (PCR) using λ DNA cl857 Sam7 (Promega, D1501) as a template and a forward primer containing two digoxigenin labels at the 5’ end ...
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bioRxiv - Genetics 2019Quote: ... Luminescence in relative light units (RLU) was measured by a Glomax microplate reader (Promega). Each construct was assayed in triplicate in 6 independent transfection experiments.