Labshake search
Citations for Promega :
1 - 50 of 1056 citations for 4' O beta Glucopyranosyl 5 O methylvisamminol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... 14 μM amino acid mix w/o methionine (Promega)) ...
-
bioRxiv - Molecular Biology 2022Quote: ... PCR amplified fragments along with O’ Generuler 1kb ladder (Promega) were resolved using 1.2% of 10X diluted GelRed™ (Biotium Inc. ...
-
bioRxiv - Developmental Biology 2021Quote: ... pTet-O-GOI-puro using the LigaFast Rapid DNA Ligation System (Promega) and heat-shock transformed into competent E ...
-
bioRxiv - Cell Biology 2023Quote: ... The antibodies used for western blotting were: anti-HALO (1:1000 in 0.5% milk TBST, 4°C o/n, Promega G9211), anti-GAPDH (1:10000 in TBST ...
-
bioRxiv - Developmental Biology 2024Quote: ... Kanamycin-resistant colonies were selected and cultured o/n purified with Plasmid Miniprep system (Promega) and verified by sequencing (Microsynth ...
-
bioRxiv - Cancer Biology 2024Quote: ... At the end of treatment cells were incubated O/N with CellTiterBlue reagent (Promega, cat. G8080). The day after ...
-
bioRxiv - Developmental Biology 2024Quote: ... 100 μM carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone (Z-VAD-FMK) (Promega, Madison, WI, USA)) ...
-
bioRxiv - Cancer Biology 2021Quote: ... and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:50 ...
-
bioRxiv - Molecular Biology 2024Quote: ... A two-stage enzymatic digestion with 2 hr lysyl endopeptidase (FUJIFILM Wako Chemicals U.S.A. Corporation) and o/n trypsin (Promega) was then performed to generate the crosslinked peptides.
-
bioRxiv - Molecular Biology 2021Quote: ... were added to the 5′ end labelled 187cspA RNA in a total volume of 10 µL in Buffer O containing 0.4 U/µL of RNasin (Promega). Complex formation was performed at 15°C or 37°C for 15 min ...
-
bioRxiv - Cancer Biology 2022Quote: ... RT) and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:100 ...
-
bioRxiv - Immunology 2020Quote: ... pH 8.0 using Vivaspin columns (3 kDa) and digested separately O/N using trypsin or chymotrypsin (Mass Spectrometry Grade, Promega) at a ratio of 1:30 (w/w) ...
-
bioRxiv - Molecular Biology 2022Quote: ... A second digestion was performed o/n at 37°C while shaking at 1,400 rpm using 500 ng of sequencing grade modified trypsin (Promega). Trypsin digested peptides were separated from Ni-NTA beads by filtering through a 0.45 µm filter Ultrafree-MC-HV spin column (Merck-Millipore).
-
bioRxiv - Cancer Biology 2020Quote: ... RT) and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:100, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:100 ...
-
bioRxiv - Immunology 2023Quote: ... and digested for 4 hrs with 2 mg/ml LysC (Wako Chemicals GmbH) in a ratio of 1:50 and O/N with 0.1 µg/µl trypsin (Promega) in a ratio of 1:50 ...
-
bioRxiv - Molecular Biology 2023Quote: ... A second digestion was performed o/n at 37°C while shaking at 1,400 rpm using 500 ng of sequencing grade modified trypsin (Promega). Trypsin digested peptides were separated from Ni-NTA beads by filtering through a 0.45 μm filter Ultrafree-MC-HV spin column (Merck-Millipore).
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... retrovirus vector containing chick U6 promoter which expresses shRNA for gfp or Plzf was transfected into M/O chicken strain-derived embryonic fibroblast cells (CEF) cultured in 60-mm dish using Fugene HD transfection regent (Promega). CEF was cultured in DMEM-high glucose containing 10% FBS ...
-
bioRxiv - Systems Biology 2022Quote: ... and digested with 3 μg LysC (Wako) O/N at 37°C and then with 3 μg of trypsin (Promega) for eight hours at 37°C following FASP procedure (Filter-aided sample preparation 48) ...
-
bioRxiv - Cell Biology 2022Quote: ... were stained with CaspACE FITC-VAD-FMK (fluorescein isothiocyanate-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone) in situ Marker (Promega, Mannheim, Germany) as per manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... Supernatants were discarded and nuclei pellets were washed once with 4 mL Nuclei EZ Lysis Buffer and once with 4 mL of Nuclei Suspension Buffer (NSB) (PBS w/o Mg and Ca; 0.01% BSA; Biolabs ref B9000S; 40 U/mL RNAsin; Promega ref N251B). Final pellets were resuspended in 0.5 mL NSB containing DAPI (1 µL at 1 mg/mL ...
-
bioRxiv - Pathology 2024Quote: ... followed by dilution to 1 M urea with 25 mM AmBic and 37 °C o/n digestion with trypsin protease (MS grade; Promega, V5280) in protein/protease ratio of 40:1 (w/w) ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins were digested O/N at 37°C with mass spectrometry grade lysyl endopeptidase LysC and sequencing grade modified trypsin (Promega LTD, UK).
-
bioRxiv - Microbiology 2021Quote: ... 4 μl 5× Buffer (Promega), 2 μl MgCl2 (25mM) ...
-
bioRxiv - Genetics 2020Quote: Primary antibodies against beta-gal (Promega), LexA (Millipore) ...
-
bioRxiv - Neuroscience 2023Quote: ... Beta III Tubulin (1:2000, Promega), and PSD-95 (1:100 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Beta-Galactosidase (1/1000e, Promega, Z3781), tropomyosin1 tm1 (1/200e ...
-
bioRxiv - Cancer Biology 2022Quote: SJ-GBM2 and SF8628 cells were used to determine if reducing WDR82 through inducible knockdown affected cell viability 1×104 cells/100μl were plated in 96-well plates with complete cell culture medium with or without Dox (2μg/ml), and subjected to 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, Promega) assay.
-
bioRxiv - Neuroscience 2024Quote: ... anti-Beta III Tubulin (Tuj1; Promega, G712A), and DAPI (Sigma ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5 and exon 4 were cloned to pGL4.23 (Promega) upstream to the minimal promoter ...
-
bioRxiv - Cell Biology 2022Quote: ... 50uL of Beta-Glo reagent (Promega, Madison, WI) and 100 uL of liquid culture was added to each well in a 96-well microplate and measured for beta galactosidase activity using the Synergy MX (BioTek ...
-
bioRxiv - Neuroscience 2021Quote: ... LacZ (Promega, Mouse anti-beta Galactosidase, 1:2000) and Nestin (Aves lab ...
-
bioRxiv - Developmental Biology 2023Quote: β-galactosidase activity was measured using Beta-Glo (Promega) essentially as described by Hook et al. ...
-
bioRxiv - Developmental Biology 2023Quote: ... mouse anti-beta galactosidase (Promega Z378825580610; 1:500), guinea pig anti-brk (provided by from Gines Morata ...
-
bioRxiv - Bioengineering 2020Quote: Cell viability was calculated with beta-Glo assay (Promega)
-
bioRxiv - Bioengineering 2021Quote: ... beta-mercaptoethanol (final concentration of 0.1 mM, Promega, USA) and Pen (1% ...
-
bioRxiv - Developmental Biology 2024Quote: ... and incubated with Anti-Beta-Galactosidase Purified Monoclonal Antibody (Promega), 1:200 in blocking solution for 2 h ...
-
bioRxiv - Molecular Biology 2024Quote: ... Beta-galactosidase activity was measured using ONPG as substrate (Promega). EGFP and dsRed accumulation was measured by western blot (see below ...
-
bioRxiv - Bioengineering 2023Quote: ... 5 µL reactions were made by adding 4 µL LgBiT (Promega, N1120), 0.5 µL 100 µM p86-R4 (Vivitide ...
-
bioRxiv - Plant Biology 2022Quote: ... The medium was aspirated and fresh medium containing 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, premixed in 5:1 ratio, Promega) was added to the treated cells ...
-
bioRxiv - Developmental Biology 2021Quote: ... and 175 μg/ml 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) (Promega). Reactions were stopped with three PBS rinses ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 175 μg/ml 5-bromo-4-chloro-3- indolyl-phosphate (BCIP) (Promega). Reactions were stopped as the signal became apparent with three PBS rinses ...
-
bioRxiv - Genetics 2021Quote: ... the following antibodies were used: mouse anti-beta-galactosidase (Promega; 1:100), mouse anti-Mmp1 (1:1:1 mixture of DSHB 3A6B4 ...
-
bioRxiv - Bioengineering 2023Quote: ... The effect on cell viability of PTNP was assessed using the MTS [(3-(4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)] assay (CellTiter 96 cell proliferation assay kit; Promega, WI, USA) (92) ...
-
bioRxiv - Plant Biology 2024Quote: ... 4 hours incubation at 37 °C) and 5 μg of trypsin (Trypsin Gold from Promega, in 50 mM TEAB pH 8.5 ...
-
bioRxiv - Bioengineering 2021Quote: ... β-galactosidase activity was measured using a Beta-Glo Assay System (E4720, Promega). The Npas2-LacZ expression data were uploaded to an online data analysis tool (CDD Vault ...
-
bioRxiv - Genetics 2021Quote: Senescence was measured using 2 assays: 1/ Beta-Glo Assay kit (Promega # E4720), according to the manufacturer’s instructions and utilizing a luciferin-galactoside substrate (6-O-β galactopyranosylluciferin) ...
-
bioRxiv - Cell Biology 2023Quote: ... the beta-galactosidase enzyme activity was measured using a kit (#E2000, Promega, USA). Luciferase readings were divided with beta-galactosidase readings to normalize for transfection differences ...
-
bioRxiv - Cancer Biology 2024Quote: ... 96-well plate (Costar) for treatment with either Beta- Glo® (Promega, #E4720) or Luciferase Assay Reagent II/LARII (Promega ...
-
bioRxiv - Microbiology 2020Quote: ... membranes were incubated in a 0.165 mg/ml BCIP (5-bromo-4-chloro-3-indolyl phosphate, Promega)/ 0.33 mg/ml NBT (p-nitroblue tetrazolium chloride ...
-
bioRxiv - Immunology 2021Quote: ... culture supernatant was removed from each well and Beta-Glo® reagent (Promega, Madison, WI) was added and the chemiluminescence activity (Relative Light Units-RLU ...