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Citations for Promega :
151 - 200 of 443 citations for 15 PGDH HPGD Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Restricted fragments to be cloned were ligated into the EcoRI and SacI restricted pBAD/His using T4 Ligase (Promega) before being heat shock-transformed into chemically competent E ...
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bioRxiv - Biochemistry 2023Quote: ... Rat Gβ1 was fused with a His-tag at the N terminus and with a SmBiT subunit (peptide 86, Promega)34 after a 15-amino acid linker at its C terminus ...
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bioRxiv - Evolutionary Biology 2019Quote: ... bulked to 200ng using human genomic DNA (Promega). All samples were sheared for 150 seconds using a Covaris E220 (Duty Factor 5% ...
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bioRxiv - Systems Biology 2020Quote: The Human cell lysate was obtained commercially (Promega) and the yeast digest was prepared as previously described23 ...
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bioRxiv - Genomics 2019Quote: ... We added 4μl pooled commercial human gDNA (Promega) to all samples to ensure total gDNA > 1μg/35μl ...
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bioRxiv - Immunology 2021Quote: ... 3) goat anti-human H+L (Promega, W403B) at 1:3,000 dilution ...
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bioRxiv - Biochemistry 2020Quote: ... Human GSK-3β was purchased from Promega (V1991).
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bioRxiv - Genomics 2020Quote: ... and human gDNA (Promega, San Luis Obispo, CA) was added to reach a total input of 3 μg/130uL for fragmentation and library prep ...
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bioRxiv - Biochemistry 2021Quote: ... and SETD2 human ORF were procured from Promega. Deletion mutants of hnRNP L ...
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bioRxiv - Biochemistry 2022Quote: Human cancer cell line digest standards K562 (Promega) and diluted to 100 microgram/mL in 50mM TEAB and labeled with TMTPro reagents according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... in the presence of 15% PEG8000 and 1 U/μl RNasin (Promega) at 18°C overnight and then at room temperature for 1 hour and inactivated at 65°C for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 15 μL of 100× diluted furimazine (Nano-Glo luciferase assay reagent, Promega) was added to each well ...
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bioRxiv - Genetics 2019Quote: ... 15 µL of 2x GoTaq Hot Start Green Master Mix (Promega, USA), 0.3 µL of 10 µM forward primer ...
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bioRxiv - Molecular Biology 2019Quote: ... or ∼20 µl of sample to 15-18 µl Fluc substrate (Promega).
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bioRxiv - Genetics 2019Quote: ... after reducing SDS-PAGE on a gradient (4-15%) polyacrylamide gel (Promega) and transfer onto nitrocellulose membrane ...
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bioRxiv - Cell Biology 2021Quote: ... and processed for reverse transcription using oligo(dT)15 primers (Promega #C110A) and SuperScript III reverse transcriptase (Thermo Fisher Scientific #18080-044) ...
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bioRxiv - Genomics 2020Quote: ... total RNA was annealed with 0.1mg/ml oligo(dT)15 primer (Promega) and cDNA synthesis was performed with M-MLV Reverse Transcriptase (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 30-50 µL of 15 ng/µL Sequencing Grade Trypsin (Promega) was added to each sample and digestion was completed overnight at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... total RNA was annealed with 0.1 mg/ml oligo(dT)15 primer (Promega) and cDNA synthesis was performed with M-MLV Reverse Transcriptase (Promega ...
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bioRxiv - Biochemistry 2019Quote: ... activating adaptors were first coupled to 15 µL of Magne HaloTag Beads (Promega) in 2 mL Protein Lo Bind Tubes (Eppendorf ...
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bioRxiv - Biochemistry 2022Quote: ... and then treated for 30 minutes with 15 units of RQ1 DNAse (Promega). The transcribed sgRNAs are subsequently PAGE purified on an 8% denaturing (7 M urea ...
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bioRxiv - Developmental Biology 2020Quote: ... 1μg of RNA was reverse transcribed with oligo(dT)15 Primers (Promega C1101)] using SuperScript III Reverse Transcriptase (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... cells were incubated for 15 min with 500nM Halo TMR ligand (Promega G8251), washed with 2i media three times followed by 30 min incubation in 2i media without the ligand to remove unbound HaloTaq ligands before fluorescence imaging in L-15 medium ...
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bioRxiv - Cancer Biology 2021Quote: ... and human Renilla luciferase (pGL4.74[hRluc/TK], Promega, E6921) were used in the study ...
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bioRxiv - Microbiology 2020Quote: ... insert (NRP1, native human ORF in pF1K, FXC23812, Promega): 5’-atagggcgaattcggtagtaaggagcgatcgc-3’ (fwd ...
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bioRxiv - Cancer Biology 2022Quote: ... were amplified by PCR from human genomic DNA (Promega), using the primers listed in Table S1 and then inserted into the psiCHECK-2 vector ...
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bioRxiv - Biochemistry 2022Quote: ... human GR and MR were cloned into pACT (Promega) as N-terminal VP16 fusions using HiFi assembly (NEB) ...
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bioRxiv - Cell Biology 2019Quote: ... A cDNA encoding human KIF1A was purchased from Promega Japan (Promega Japan ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Human PAR2 with N-terminal nano-luciferase (nLuc, Promega) and C-terminal enhanced Yellow Fluorescent Protein (eYFP ...
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bioRxiv - Molecular Biology 2021Quote: ... or goat anti-Human IgG (H+L) (Promega, W4031) antibodies were added for 30 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: 1 ml of recombinant His-MBP-AlpA and His-MBP at a concentration of 10 μM was incubated with 100 μl MagneHis™ Ni Particles (Promega) equilibrated with pulldown-buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... The plates were read within 15 minutes (GloMax Discover luminometer, Promega, Madison, WI, USA) equipped with 450 nM BP filter (donor ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were incubated for 15 min with the HaloTag® TMR G8252 ligand (Promega) in fresh medium ...
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bioRxiv - Cell Biology 2021Quote: ... 9 μl purified RNA was incubated with 1 μl Oligo(dT)15 primers (Promega) at 80°C for 3 min ...
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bioRxiv - Microbiology 2021Quote: ... PFGE of total DNA either undigested or digested 15 min with S1 nuclease (Promega) was carried out as described by Freitas et al ...
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bioRxiv - Biochemistry 2022Quote: ... to 100 µl add 15 µl DNase I and 1.5 µl RNASin Plus (Promega) before use) ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... for 15 minutes at 37°C before lysing with reporter lysis buffer (Promega, E397A). Samples were spun at 15,000 rpm for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 15 ng of pRL-null Renilla luciferase control reporter (#E2271, Promega; for background normalization), and compensated with empty vector control (pCMV2c ...
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bioRxiv - Developmental Biology 2023Quote: Carm1 promoter region (-630 to +15) was cloned to pGL4 luciferase reporter vector (Promega) to be the Carm1-reporter ...
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bioRxiv - Biochemistry 2023Quote: ... peptides were collected as described in ProteaseMax application note (Promega, TB373, Rev. 2/15), and purified using Pierce C18 Spin Tips (Thermo Fisher PI84850) ...
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bioRxiv - Molecular Biology 2021Quote: The CD19 minigene was amplified from human genomic DNA (Promega) with the primers 5’-catAAGCTTgaccaccgccttcctctctg-3’ and 5’-catGAATTCNNNNNNNNNNNNNNNGGATCCttcccggcatctccccagtc-3’ ...
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bioRxiv - Neuroscience 2020Quote: ... the human CB1R was cloned into the pCI vector (Promega), and eCB2.0 and eCBmut were cloned into the peGFP-C1 vector (Takara) ...
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bioRxiv - Immunology 2022Quote: ... and a β-globin calibration curve (human genomic DNA [Promega]) was concurrently evaluated on each plate ...
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bioRxiv - Microbiology 2020Quote: ... The human codon-optimized NanoLuc Luciferase reporter gene (Nluc, Promega) was inserted in place of nucleotides 1-100 of the nef-gene ...
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bioRxiv - Genomics 2022Quote: ... 10 ng/mL recombinant human basic fibroblast growth factor (Promega), and 1 μM dexamethasone (Sigma-Aldrich) ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... donovani BPK282/0 cl4 promastigote DNA with human DNA (Promega) from 1/15 to 1/150000 (Leishmania:human ...
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bioRxiv - Biochemistry 2020Quote: hnRNP L and SETD2 human ORF were procured from Promega. Deletion mutants of hnRNP L and SETD2 were constructed by PCR (Phusion polymerase ...
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bioRxiv - Immunology 2020Quote: ... The human codon-optimized nanoluc Luciferase reporter gene (Nluc, Promega) was inserted in place of nucleotides 1-100 of the nef-gene ...
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bioRxiv - Molecular Biology 2022Quote: ... Serial dilutions of genomic DNA from human placenta (G1471, Promega) were used as a standard for quantification and their concentration.
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bioRxiv - Microbiology 2022Quote: ... falciparum 3D7 DNA with commercially available human DNA (Promega, G304A) to a total amount of 400ng DNA ...