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Citations for Promega :
551 - 600 of 894 citations for 10 Methylundecanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Firefly luciferase activities were measured in 10 μl of lysate using 50 μl luciferase assay substrate (Promega). To normalise transfection efficiencies β-Galactosidase levels were measured using the β-Galactosidase Enzyme Assay System (Promega) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were verified to be mycoplasma-negative and genetically authenticated by STR profiling (Gene Print 10, Promega). HBEKT cells were maintained in keratinocyte serum free media with supplementation according to Lonza protocol ...
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bioRxiv - Biochemistry 2021Quote: ... prior to the addition of 80μl digestion buffer (10% 2,2,2-Trifluoroethanol (TFE)/100 mM NH4HCO3) containing 4μg Trypsin-gold (Promega, V5280) and 4μg Lys-C (Wako ...
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bioRxiv - Microbiology 2021Quote: ... 10 and 13 days of incubation in a GloMax® 96 or GloMax Navigator Microplate Luminometer (Promega) with 1.0 s integration per well ...
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bioRxiv - Cell Biology 2022Quote: ... The columns were transferred to new tubes and incubated with 10 ng/μL sequencing-grade trypsin (Promega) overnight at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Residual genomic DNA was removed by treating with 10 units/sample of RNAse-free RQ1 DNAse (Promega) for 1h at 37°C followed by phenol-chloroform extraction and ethanol precipitation ...
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bioRxiv - Cell Biology 2023Quote: ... Cells expressing HaloTag constructs were incubated overnight with 10-100 nM Janelia Fluor 646 HaloTag ligand (Promega) (61) ...
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bioRxiv - Molecular Biology 2023Quote: ... The columns were transferred to new tubes and incubated with 10 ng/μl sequencing-grade trypsin (Promega) (49) ...
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bioRxiv - Biochemistry 2023Quote: A mixture of 10 ng of biotinylated BSA digest and 5 µg of K562 cell digest (Promega) was added to streptavidin beads (10 µL of suspension used ...
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bioRxiv - Biochemistry 2023Quote: ... prior to running on a denaturing urea 10% polyacrylamide gel and stained with Diamond DNA stain (Promega). The experiment was repeated in triplicate and quantified using BioRad Image Lab software.
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bioRxiv - Biochemistry 2023Quote: ... and used 1 μL of cDNA for 10 μL of PCR using 2x GoTaq master mix (Promega) and 250 nM of forward and reverse primer sets with conditions recommended by the manufacturer ...
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bioRxiv - Physiology 2023Quote: ... Each 10 µl PCR reaction consisted of 5 µl of GoTaq® qPCR Master Mix (Promega Corporation), 0.5 µl of 10 µM forward primer ...
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bioRxiv - Molecular Biology 2023Quote: The reaction mixture (10 μl) was prepared with 5 μl of rabbit reticulocyte lysate nuclease-treated (Promega), 2 μl of H2O ...
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bioRxiv - Genomics 2023Quote: ... The aqueous layer was carefully removed to a new tube and 10% weight/volume PEG8000 (Promega #V3011) was added and incubated on ice for 1 hour before centrifugation at 20,000 x g for 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Identity of all cell lines were confirmed by short tandem repeat testing (GenePrint 10 ID System; Promega) (Supplementary Table 12) ...
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bioRxiv - Plant Biology 2023Quote: ... 0.5 μl of the DNA extract was added to 10 μl of 5X GoTaq Reaction Buffer (Promega) and 1 μl of each primer (10 μM ...
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bioRxiv - Genetics 2023Quote: ... 50 µL of ATDC5 Complete Media and 10 µL of CellTiter 96 AQueous One Solution (Promega G3580) were added to each well ...
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bioRxiv - Genomics 2023Quote: ... Primer efficiency was calculated using a 10-fold serial dilution of Human Mixed Genomic DNA (Promega G3041). For primer sequences and calculated efficiencies ...
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bioRxiv - Microbiology 2024Quote: ... diluted 10 x into sterile water and processed with the Nano-Glo® Luciferase Assay System (Promega). The bioluminescence was measured using a Spark multimode microplate reader (Tecan).
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bioRxiv - Systems Biology 2024Quote: ... Cells were first transfected with 3′UTR luciferase constructs (10 ng) using FuGENE HD transfection reagent (Promega) for 4 hours then transfected with Dharmacon miR-199a-5p mimic (50 nM ...
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bioRxiv - Cell Biology 2024Quote: ... alkylated with 10 mM iodoacetamide and subject to sequential protein digestion with Lys-C and trypsin (Promega). Digestion was halted with formic acid ...
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bioRxiv - Cell Biology 2019Quote: ... Gel pieces were rehydrated in 10-15 µL of solution containing 20 ng/µL trypsin (Promega, Madison, WI) in 50 mM ammonium bicarbonate for 15 min ...
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bioRxiv - Cell Biology 2020Quote: ... room temperature for 30 min) and digested in 10 % acetonitrile and 10mM ammonium bicarbonate with modified trypsin (Promega) at a 1:10 enzyme-to-substrate ratio ...
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bioRxiv - Genomics 2020Quote: ... PCR amplification was performed in a 10- μL reaction mixture containing 5 μL of GoTaq Master Mix (Promega), 5 pmol FAM-labeled universal primer (5′ - FAM-gctacggactgacctcggac-3′) ...
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bioRxiv - Bioengineering 2020Quote: The Round 1 eluted pool was PCR amplified for 10 cycles with GoTaq Hot Start Polymerase (Promega, M5001) (1X Colorless GoTaq Flexi Buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... The identity of all cell lines was confirmed by DNA fingerprinting analysis utilizing the GenePrint 10 System (Promega).
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bioRxiv - Plant Biology 2020Quote: ... in 10 µL of reaction medium (1.5 mM MgCl2, 100 mM KCl, 0.1 U RNasin Ribonuclease Inhibitor (Promega), 20 mM HEPES-KOH (pH 7.0) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of dNTP mix (containing 10 mM of each dNTP) and 1 μl RNasin ribonuclease inhibitor (Promega). The reaction was carried out at 42°C for 50 mins ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of dNTP mix (containing 10 mM of each dNTP) and 1 μl RNasin ribonuclease inhibitor (Promega) in a total reaction volume of 25 μl ...
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bioRxiv - Genomics 2022Quote: ... Supernatant was removed and nuclei were incubated in ice cold DNase reaction mix (10 µL RQ1 DNase, (Promega), 10 µL 10x DNase buffer ...
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bioRxiv - Genetics 2020Quote: ... cells were changed into GloSensor equilibration medium (10% fetal bovine serum and 2% GloSensor assay reagent (Promega E1290) in CO2-independent medium (Gibco 18045-088) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM EDTA pH 8.0,10% glycerol) for 10 min on ice in the presence of protease inhibitors (G6521, Promega), then were homogenized using a glass homogenizer ...
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bioRxiv - Plant Biology 2021Quote: ... The powder was resuspended in 30 mL of Extraction Buffer 1 (10mM Tris-HCl pH8; 0.4M sucrose; 10 mM MgCl2; 5 mM BME; 0.2 mM PMSF; RNasin PROMEGA) and was filter thought a Nylon membrane of 150 μm and centrifuged at 2000 g for 20 min at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... The eluent was equally divided into three for digestion with either: 10:1 (w/w) Trypsin Gold (Promega), 7.5:1 (w/w ...
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bioRxiv - Microbiology 2019Quote: ... We reverse-transfected cells in each well with 10 ng of pGL4.23-CMV/NP-NLuc or pNL1.1/NLuc (Promega) negative control and 100 ng of pGL4.23-CMV/NP-HaloTag or pcDNA3.3/eGFP negative control ...
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bioRxiv - Neuroscience 2021Quote: ... gel pieces were rehydrated with 10 µL 50 mM NH4HCO3 containing 25 µg/mL trypsin (Trypsin Gold, Promega) and incubated for 15 min on ice ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested 10-hours post-transfection and the luciferase reads were quantified using Dual-Luciferase reagents (Promega) as per manufacturer’s protocol on EnSpire multimode plate reader (Perkin Elmer).
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bioRxiv - Genetics 2020Quote: ... The 10 µL Zn-finger PCR reaction was prepared containing of 2 µL of 5X reaction buffer (Promega), 0.8 µL of 2.0 mM MgCl2 ...
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bioRxiv - Molecular Biology 2021Quote: ... The reaction was carried out in 10 μl of Buffer M containing 0.4 U/µL of RNasin (Promega) in the presence of 0.02 μM cspA mRNA ...
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bioRxiv - Genomics 2021Quote: ... HMW DNA was resuspended in 10 mM Tris pH 8 and quantified by Quantus™ Fluorometer (Promega Inc). The DNA size and integrity were assessed on TapeStation™ instrument (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... The concentrated phages were next treated for 30 min at 37°C with 10 μL of DNAse (Promega) and 2.5μL of RNAse (Macherey-Nagel ...
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bioRxiv - Cancer Biology 2022Quote: ... and 100 μl of 50 mM ammonium bicarbonate buffer as well as 10 μl of protease solution (Promega Trypsin/Lys-C Mix ...
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bioRxiv - Cell Biology 2022Quote: ... room temperature for 30 min) and digested in 10% acetonitrile and 10mM ammonium bicarbonate with modified trypsin (Promega) at a 1:10 enzyme-to-substrate ratio ...
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bioRxiv - Molecular Biology 2023Quote: A total of 10 µg RNA was taken from each sample for reverse transcription (RT) using GoScript (Promega) reverse transcriptase (5 µg for RT ...
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bioRxiv - Microbiology 2023Quote: Total DNA was extracted from 10 ml bacterial overnight grown cultures using Wizard Genomic DNA Purification Kit (Promega) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 1 μL of each primer (10 μM) and 12.5 μL of GoTaq Green Master mix (Promega®, USA). The PCR was run in a thermocycler (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... The concentrated phages were next treated for 30 min at 37°C with 10 µl of DNAse (Promega) and 2.5 µl of RNAse (Macherey-Nagel ...
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bioRxiv - Microbiology 2023Quote: Cell supernatants were collected and stored in LDH storage buffer + Triton 10% as per the manufacturer’s instructions (Promega). Supernatants were inactivated for 2 hours at room temperature prior to usage outside the BSL-3 ...
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bioRxiv - Cancer Biology 2023Quote: ... and cell culture identity verification was done by short tandem repeat profiling (GenePrint® 10 System, Promega #B9510).
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed using 10 ng of cDNAs (RNA equivalent) and the GoTaq DNA polymerase (Promega, # M8295). For each gene ...