Labshake search
Citations for Promega :
4651 - 4700 of 5372 citations for 6 9 Methano 4 1 benzoxazepin 2 3H one octahydro 3 methyl 3 α 5a bta 6 bta 9 bta 9a bta 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: Protein pellets were dissolved in digestion buffer and digested by 1% ProteaseMAX detergent (Promega, Madison, WI, USA) and Trypsin/Lys-C Mix (Promega ...
-
Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesisbioRxiv - Molecular Biology 2023Quote: ... Tryptic digestion was performed by incubating beads with 1 μg MS-grade TPCK trypsin (Promega, Madison, WI) dissolved in 200 μl of 50 mM ammbic (pH 8.3 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Diluted RNA was assayed for AGO2 transcript using the GoTaq 1-Step RT-qPCR System (Promega A6020) with primers CTGGCTCCAGGGGACAAG (Forward primer ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were incubated with a 1:100 dilution of primary mouse anti-NLuc antibody (Promega, cat#7000) in PBS overnight at 4 °C ...
-
bioRxiv - Plant Biology 2024Quote: ... alkylated (55 mM Iodoacetamide; 1h @ 23°C) and digested in solution with 1:100 trypsin (V5113; Promega). Peptides were acidified to 0.5% (v/v ...
-
bioRxiv - Cell Biology 2024Quote: ... the reaction mixtures for in vitro transcription were treated with RQ-1 RNase-free DNase I (Promega). Synthesized mRNAs were treated with phenol–chloroform to remove protein components ...
-
bioRxiv - Genomics 2024Quote: ... The master mixtures consisted of 1 x Taq&Go G2 Hot Start colorless PCR Master Mix (Promega), 0.5 μM of each primer ...
-
bioRxiv - Developmental Biology 2024Quote: ... The secondary antibody used was HRP-conjugated goat/rabbit/donkey anti-mouse IgG (1 : 10000, Promega, W402B).
-
bioRxiv - Plant Biology 2024Quote: ... 1 µg or total RNA was used for the cDNA synthesis (GoScript ™ Reverse Transcription System, Promega). Quantitative real-time PCRs were performed in the Agilent AriaMx System using the GoTaq® qPCR Master Mix (Promega ...
-
bioRxiv - Microbiology 2024Quote: ... viral RNA was reverse transcribed and quantified using GoTaq Probe 1-Step RT-qPCR System kit (Promega) in the presence of the specific SARS-CoV-2 probe (5′-6-FAM-ACT TCC TCA AGG AAC AAC ATT GCC A-BHQ-1-3′ ...
-
bioRxiv - Molecular Biology 2024Quote: ... 50ul (50.000 cells) were transferred to a new 1.5ml Eppendorf tube and 0.5ul 1% Digitonin (Promega #G9441) was added by pipetting well few times ...
-
bioRxiv - Neuroscience 2021Quote: ... Reverse transcription was performed on 1 μg of RNA with random hexamer primers using MMLV-RT enzyme (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2021Quote: ... 1 μg of DNA-free RNA was then used for reverse transcription using M-MLV Reverse Transcriptase (Promega) in the presence of RiboLock ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Sperms were released from antheridiophores by applying ddH2O supplemented with RNasin® Ribonuclease Inhibitor (1 u/μl, Promega), collected in a 1.5 mL tube ...
-
bioRxiv - Pathology 2022Quote: ... A total of 1 µg of extracted RNA was transcribed into cDNA using GoScript Reverse Transcription Kit (Promega). Quantitative real-time PCR was performed using FastStart SYBR Green Master mix (Roche ...
-
bioRxiv - Neuroscience 2022Quote: ... The sample solutions were diluted to 1 M urea with 50 mM ammonium hydrogen carbonate and trypsin (Promega) was added at an enzyme-to-substrate ratio of 1:50 ...
-
bioRxiv - Neuroscience 2021Quote: ... cDNA synthesis was subsequently performed using 1 μg total RNA and oligo-dT primers (C110A, Promega, Madison, WI). Transcript levels were assessed via real-time quantitative polymerase chain reaction (qPCR ...
-
bioRxiv - Molecular Biology 2020Quote: Transfection of 1 µg of each individual DNA was performed in six-well plates using FuGENE HD (Promega) for HeLa S3 cells according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2020Quote: Cells were washed twice with PBS then lysed directly in wells using 1× cell culture lysis reagent (Promega). Cell lysates were clarified by centrifugation ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were diluted with three volumes digestion buffer (100 mM Tris/HCl pH 8.5, 1 mM CaCl2) and 0.25 μg Trypsin (Sequencing Grade, Promega) were added and incubated o/n at 37 °C ...
-
bioRxiv - Cancer Biology 2019Quote: ... beads were washed two extra times in TBS and resuspended in digestion buffer (2M urea, 50Mm Tris-HCL pH7.5, 1 mM DTT) with 1µg of porcine trypsin MS-grade (Promega) for digestion ...
-
bioRxiv - Developmental Biology 2019Quote: ... HRP coupled secondary antibodies (1/2,000, sigma) were used and blots were developed using an ECL kit (Promega).
-
bioRxiv - Cancer Biology 2019Quote: ... washed two times in lysis buffer and three times in TBS and resuspended in digestion buffer (2M urea, 50Mm Tris-HCL pH7.5, 1 mM DTT) with 1µg of porcine trypsin MS-grade (Promega) for digestion ...
-
Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: Eluted protein samples from BIA experiment were digested in gel with 1 µg of trypsin (sequence grade, Promega) at 37°C OVN ...
-
bioRxiv - Cell Biology 2019Quote: ... Tryptic digestion was performed by incubating the beads with 1 µg MS-grade TPCK trypsin (Promega, Madison, WI) dissolved in 200 μl of 50 mM ammonium bicarbonate (pH 8.3 ...
-
bioRxiv - Bioengineering 2020Quote: ... Total RNA was reverse transcribed using 0.5–1 µg of total RNA and ImProm-II Reverse Transcriptase (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2020Quote: The Round 1 eluted pool was PCR amplified for 10 cycles with GoTaq Hot Start Polymerase (Promega, M5001) (1X Colorless GoTaq Flexi Buffer ...
-
bioRxiv - Neuroscience 2020Quote: ... the following primary antibodies were used at the indicated concentrations: 1:10’000 mouse anti-β3-Tubulin (Promega, G712A), rabbit anti-p-ERK T202/Y204 (CST ...
-
bioRxiv - Microbiology 2020Quote: ... cDNA was diluted 1:5 before quantitative analysis by qPCR was done using GoTaq qPCR Master Mix (Promega) following manufacturer’s recommendations ...
-
bioRxiv - Biochemistry 2022Quote: ... The eluent was equally divided into three for digestion with either: 33:1 (w/w) trypsin gold (Promega), 25:1 (w/w ...
-
bioRxiv - Molecular Biology 2022Quote: ... The urea concentration was then diluted with 17 μl of 50 mM HEPES pH 8.5 and after adding 1 μl of 0.2 mg/ml trypsin (Promega) in 0.2 mM HCl the digestion continued overnight at 37°C with interval mixing at 2000 RPM for 30 s every 15 minutes ...
-
bioRxiv - Microbiology 2021Quote: ... sample-filters were incubated in a wet-cell adding 1:50 w/w trypsin: protein ratio (Promega, V5111) solubilized in 120 µl 50 mM TEAB ...
-
bioRxiv - Plant Biology 2021Quote: ... filled with 175 μL 0.5x MS-agar medium 1% sucrose and 35μL (50 μg/mL) of Beetle Luciferin (Promega) dissolved in DMSO ...
-
bioRxiv - Molecular Biology 2020Quote: ... 293T cells were transfected in 12 well plates with 1 ug of pcDNA3.4 POLGARF or pcDNA3.4 Timer (as a negative control) with Fugene transfection reagent (Promega) according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies were either goat anti-mouse IgG HRP or goat anti-mouse IgG HRP (Promega; 1:10,000) respectively.
-
bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by incubation with either sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin), chymotrypsin sequencing grade (1/50 ...
-
bioRxiv - Biochemistry 2021Quote: ... The sample was four-fold diluted and the protein digested in presence of 0.2 M Tris-HCl buffer pH 8.0 and 1 μg of sequencing grade trypsin (Promega) previously dissolved in 20 mM acetic acid ...
-
bioRxiv - Biochemistry 2020Quote: ... and added to 50-100 µL ribosome-coupled anti-Flag M2 agarose beads and 1 µL RNasin (Promega) per reaction ...
-
bioRxiv - Neuroscience 2022Quote: ... then digested at a 1:25 enzyme:protein ratio with proteomics grade trypsin/rLysC (V5071; Promega, Madison, WI, USA) overnight at 37°C according to the SP3 method (Hughes et al ...
-
bioRxiv - Cancer Biology 2022Quote: ... The first step of PCR was carried out with 1 µl of cDNA and DNA polymerase GoTaq (Promega) according to the manufacturer’s instructions and consisted of 95 °C for 5 min and 15 cycles of 95 °C for 30 seconds ...
-
bioRxiv - Biophysics 2019Quote: ... The sample solutions were diluted to 1 M urea with 50 mM ammonium hydrogen carbonate and trypsin (Promega) was added at an enzyme-to-substrate ratio of 1:50 ...
-
bioRxiv - Cell Biology 2019Quote: DNA from DLD-1 and nucleoporins-targeted cells was extracted with Wizard® Genomic DNA Purification Kit (Promega). Clones were genotyped by PCR for homozygous insertion of tags with two sets of primers listed in Supplementary Table 5.
-
bioRxiv - Cell Biology 2020Quote: DNA from DLD-1 and nucleoporins-targeted cells was extracted with Wizard® Genomic DNA Purification Kit (Promega). Clones were genotyped by PCR for homozygous insertion of tags with two sets of primers listed below.
-
bioRxiv - Synthetic Biology 2020Quote: ... a 1 mL aliquot was taken for genomic isolation through the Maxwell kit (Promega, Cat# AS1490, Madison WI). Successful integration was verified through qPCR ...
-
bioRxiv - Synthetic Biology 2020Quote: ... a 1 mL aliquot was taken for genomic isolation using the Maxwell kit (Promega, Cat# AS1490, Madison WI). Successful integration was verified using qPCR ...
-
bioRxiv - Molecular Biology 2020Quote: ... 8 μl 5x PNK pH 6.5 buffer [350 mM Tris-HCl pH 6.5, 50 mM MgCl2, 5 mM DTT], 1 μl PNK enzyme [NEB], 0.5 μl RNasin [Promega] ...
-
bioRxiv - Plant Biology 2021Quote: ... The powder was resuspended in 30 mL of Extraction Buffer 1 (10mM Tris-HCl pH8; 0.4M sucrose; 10 mM MgCl2; 5 mM BME; 0.2 mM PMSF; RNasin PROMEGA) and was filter thought a Nylon membrane of 150 μm and centrifuged at 2000 g for 20 min at 4 °C ...
-
bioRxiv - Biochemistry 2020Quote: ... The eluent was equally divided into three for digestion with either: 10:1 (w/w) Trypsin Gold (Promega), 7.5:1 (w/w ...
-
bioRxiv - Immunology 2020Quote: ... the membrane was incubated with HRP-conjugated goat anti-rabbit IgG diluted 1:2000 (Promega, Madison, Wisconsin, USA) for 2 h at RT ...
-
bioRxiv - Immunology 2021Quote: ... qRT-PCR was performed in 12 μL reactions using GoTaq Probe 1-step RT-qPCR System (Promega, US) according to the manufacturer’s instructions ...