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Citations for Promega :
4451 - 4500 of 4642 citations for Mouse Vasoactive intestinal polypeptide receptor 2 VIPR2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... Luciferase activity was analysed using a luminometer and a Dual-Glo Luciferase assay kit according to the manufacturer’s instructions (Promega K.K., Tokyo, Japan). The firefly luciferase values were normalized to those of Renilla luciferase ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from approximately 106 cells via the Maxwell 16 LEV simply RNA Tissue Kit (Cat. #AS1280, Promega, Madison, WI) on a Maxwell 16 Instrument (Cat ...
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bioRxiv - Microbiology 2019Quote: ... antibody-dependent cell-mediated cytotoxicity (ADCC) bioassays (29) were performed as prescribed by the manufacturer (ADCC reporter bioassay, complete kit, Promega, cat # G7010). In brief ...
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bioRxiv - Genetics 2020Quote: ... Dog DNA was isolated from clinical blood samples provided by CEU Cardenal Herrera Veterinary Hospital of University of Valencia using the Wizard Genome DNA Purification Kit (Promega, WI, USA). The use of dog blood samples was approved by the Animal Experimentation Ethics Committee of CEU Cardenal Herrera University of Valencia ...
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bioRxiv - Genetics 2020Quote: The RNA was isolated using a Maxwell® 16 AS2000 instrument with a Maxwell® 16 LEV simply RNA Tissue Kit (both Promega) following the recommended protocol ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was extracted from whole blood obtained from GALA II study subjects using the Wizard Genomic DNA Purification kits (Promega, Fitchburg, WI), and DNA was quantified by fluorescent assay ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from this concentrated virus sample using a modified protocol with the Wizard Genomic DNA Purification Kit A1120 (Promega, Madison, WI). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: In vitro translation of full-length SREBP1a and full-length SREBP1c was carried out using the TNT rabbit reticulocyte lysate translation kit (Promega, Wisconsin, USA) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was isolated from tomato freeze-dried tissue (seedlings, leaves or fruit pericarp) using the Maxwell® RSC Plant RNA Kit with the Maxwell® RSC Instruments (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from these bacterial suspensions using a Maxwell® 16 automated nucleic acid purification system and the Maxwell® tissue DNA purification kit following the manufacturer’s instructions (Promega, UK). Purified DNA extracts were treated with RNase (New England BioLabs ...
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bioRxiv - Physiology 2020Quote: ... The transferase dUTP Nick End Labeling (TUNEL) assay was performed on cryosections of rat eyes using the TUNEL apoptosis detection kit (DeadEnd Fluorometric TUNEL System; Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Viral RNA was isolated from plasma samples using the Maxwell 16 Viral Total Nucleic Acid Purification kit on the Maxwell 16MDx instrument (Promega, Madison WI). Viral RNA was then quantified using a highly sensitive qRT-PCR assay based on a previously published protocol115.
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bioRxiv - Plant Biology 2019Quote: Extraction of genomic DNA from infected and healthy plant materials was performed using the Wizard Genomic DNA purification kit (Promega, Madison, WI); DNA was isolated from bacterial cultures using the Ultra Clean Microbial DNA Isolation kit (Mo Bio. ...
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bioRxiv - Genomics 2021Quote: ... germinated urediniospores and from rust infected leaves at 6 and 9 days post inoculation (dpi) was extracted using the Promega Maxwell® RSC Plant RNA Kit with a Maxwell® RSC instrument (Promega.com.au). The spores were induced for germination by placing on the surface of milli-Q water ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Evolved strains were thawed and grown overnight in 150 ml of liquid medium and DNA was extracted using the Wizard Genomic DNA Purification Kit from Promega (WI, USA). One DNA sample (20 to 60 µg ...
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bioRxiv - Evolutionary Biology 2021Quote: Viral RNA (vRNA) was extracted from 100 μl of VTM using the Viral Total Nucleic Acid Purification kit (Promega, Madison, WI, USA) on a Maxwell RSC 48 instrument and eluted in 50 μL of nuclease-free H2O.
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bioRxiv - Cell Biology 2020Quote: The cells in the outer wells of transwell cultures were assayed for viability with CellTiter-Blue Cell Viability Assay kit (Promega, Madison, WI). Viability analyses followed manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Primary human airway epithelial cells were homogenized in homogenization solution supplemented with 1-thioglycerol and lysed by addition of lysis buffer (Maxwell 16 LEV simplyRNA cells kit; Promega #AS 1270). Prior to RNA extraction ...
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bioRxiv - Microbiology 2019Quote: ... RNA was extracted using the Maxwell 16 LEV simplyRNA tissue kit in combination with the Maxwell nucleic acid extraction robot (Promega, WI, USA), according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... the Wizard Genomic DNA Purification Kit was used including both lysozyme and lysostaphin (10 mg/ml each) (Ref. A1120, Promega Corporation, Spain); PacBio sequencing ...
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bioRxiv - Microbiology 2020Quote: ... All PCR products were purified with an AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA) and quantified using a QuantiFluor™-ST (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... was performed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay kit using manufacturer’s instructions from Promega (Madison, WI, USA). Vero E6 cells were seeded in 96-well plates at a density of 10,000 cells/well ...
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bioRxiv - Microbiology 2020Quote: The cytotoxicity of fenbendazole to macrophages was determined using the Cytotox 96® non-radioactive cytotoxicity assay kit (Promega, catalog number G1780), following the manufacturer’s recommendations ...
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bioRxiv - Genetics 2019Quote: Genomic DNAs were isolated from whole blood EDTA samples with a Maxwell 16 instrument using Maxwell 16 LEV Blood DNA kits according to the manufacturer’s protocol (Promega, Madison, WI, USA). These genomic DNAs were used as templates for PCR with a panel of primers that flank the highly polymorphic peptide binding domains encoded by exon 2 of class I (Mamu-A ...
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bioRxiv - Genomics 2021Quote: ... and cleaned-up PCR product concentrations were measured using a QuantiFluor® dsDNA System assay kit on a Quantus™ Fluorometer (Promega). DNA library preparation was performed using the Ligation sequencing kit SQK-LSK309 (Oxford Nanopore Technologies ...
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bioRxiv - Microbiology 2022Quote: ... 100 μl of these monocultures was added to the wells of an opaqued walled 96 well plate and ATP was measured using a bioluminescent kit as recommended by the manufacturer (BacTiter-Glo Microbial Cell Viability Assay, Promega, Madison, WI). An ATP standard curve was prepared using purified ATP (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: Total genomic DNA was isolated from a piece of fin or muscle tissue using a Wizard Genomic DNA Purification kit (Promega, Tokyo, Japan) or DNeasy blood and tissue kit ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor pieces were placed in homogenizer tubes containing ceramic beads along with lysis buffer from the Total RNA Purification Kit (Norgen, 37500) and RNAse inhibitors (10 μl/mL β-mercaptoethanol and 200 units/mL RNAsin Plus (Promega, N2615)) ...
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bioRxiv - Immunology 2022Quote: Cytotoxicity was determined by the lactate dehydrogenase (LDH) release-based assay using the CytoTox 96® non-radioactive cytotoxicity assay kit (Promega Corporation) as previously described.11 Live-cell imaging assays were performed using the IncuCyte platform (Essen BioScience) ...
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bioRxiv - Developmental Biology 2022Quote: Genomic DNA was prepared from RV free wall and HD-area pieces using the Wizard® Genomic DNA Purification Kit (Promega, France). Samples were submitted to polymerase chain reaction analysis ...
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bioRxiv - Genetics 2022Quote: ... Total RNA was extracted from 14-day-old seedlings (12-days old for the cordycepin experiments) using the Maxwell 16 LEV Plant RNA Kit and Maxwell 16 Instrument (Promega, Madison, WI). For RT-PCR and qRT-PCR ...
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bioRxiv - Evolutionary Biology 2022Quote: ... gDNA was isolated from overnight cultures grown in the respective evolution medium inoculated by single evolved colonies using Wizard Genomic DNA purification kit (Promega, Madison, Wisconsin). We performed qPCR using Promega qPCR 2x Mastermix (Promega ...
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bioRxiv - Microbiology 2023Quote: ... United Kingdom) and genomic DNA from bacterial colonies was obtained using the Promega Wizard Genomic DNA Purification Kit (Promega, Madison WI, USA). DNA concentrations were measured with Nanodrop (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted at the NCPHL from subcultures inoculated with single bacterial colonies and grown in nutrient agar (Oxoid, USA) at 37°C overnight according to the manufacturer instructions (Wizard® Genomic DNA Purification kit, Promega, UK). Genomic DNA samples (derived from 10 environmental and 250 clinical samples ...
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bioRxiv - Physiology 2024Quote: RNA was isolated from islets from ∼15-week-old male mice (WT and Kcnk16 L114P (L/P)) using Maxwell® 16 LEV simplyRNA Purification Kits (Catalog no. AS1280, Promega, USA). RNA integrity was analyzed using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Cell Biology 2024Quote: ... the supernatant was measured for LDH (Lactate dehydrogenase) release using the kit of CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... Complementary DNA (cDNA) synthesis was performed with oligo-dT primers and random hexamers using the First Strand cDNA Synthesis Kit (Promega, Madison WI) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... tissue homogenization was carried out by bead beating and immediately followed by RNA extraction using the Maxwell® RSC simplyRNA Tissue Kit (Promega AS1340) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: Complete genomic DNA was extracted from fresh overnight growth on Nutrient agar (Oxoid, Hampshire, England, U.K) using a Wizard genomic DNA purification kit (Promega Corporation, Madison, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: The extent of ADCC activation induced by human γ-globulins was evaluated with the use of an ADCC Reporter Bioassay (Core Kit; Promega, G7010) and the EnSpireMultimode Plate Reader (PerkinElmer) ...
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from 1×106 cryopreserved lymph node (LN) mononuclear cells (LNMC) isolated from LN biopsies using the SimplyRNA Tissue kit for the Maxwell RSC instrument (Promega, Madison, WI). RNA was then tested for both SIV gag and a cellular gene ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from Tetrahymena WT (SB210, with or without BrdU-labeling) and ΔAMT1 cells using Wizard® Genomic DNA Purification Kit (Promega, A1120), sheared to 3-5kb in length with Megaruptor (Diagenode Diagnostics) ...
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bioRxiv - Synthetic Biology 2023Quote: ... lysed with 1X Passive Lysis Buffer then analysed using the Dual-Luciferase Assay kit on a GloMax multi+ plate reader (Promega, Southampton, UK).
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bioRxiv - Microbiology 2023Quote: ... Samples where a 1 log decrease in RLU compared to the no-serum virus-only control was observed were then selected for cytotoxicity assay using Cell Titre Glo kit (Promega, Southampton, UK) and light microscopy to verify viability of cells prior to undertaking PVNA (data not shown) ...
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bioRxiv - Biophysics 2023Quote: ... Apoptosis and necrosis assays were performed with a RealTime-Glo Annexin V Apoptosis and Necrosis Assay kit (JA1011, Promega, Madison, WI, USA) at the indicated times after changing the culture medium ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA from MB cells treated with RA for 48 h was extracted using the SV Total RNA Isolation System kit (Promega, Madison, USA), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... We then performed first-strand cDNA synthesis from 400 ng total RNA in 20 uL reactions using Promega GoScript Reverse Transcription Kit (Promega, catalog # A5000).
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bioRxiv - Biochemistry 2023Quote: ... Enzymatic activities of KARS wild-type and mutants were determined by measuring AMP production and ATP consumption with AMP-Glo Assay Kit (Cat#V5011, Promega, Madison, USA) and ATP Bioluminescent Assay Kit (Cat#FLAA ...
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bioRxiv - Bioengineering 2023Quote: ... The total ATP contents in static and dynamic cultured Hep3B cell spheroids were measured with the CellTiter-Glo® assay kit (Promega, G9681). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were lysed 24 h after transfection and luciferase activity was measured with the Dual-Luciferase Reporter Assay kit (Promega Cat. # E1960) according to the manufacturer’s protocol using a FlUOstar plate reader (BMG Labtech) ...