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Citations for Promega :
401 - 450 of 5336 citations for hsa mir 301a 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Plates containing the different transfected cells were incubated for 24 hours at 37°C before the luciferase activity was measured using the Dual-Glo luciferase detection kit (Promega: E2920).
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bioRxiv - Physiology 2021Quote: ... Extracts were used for quantification of luciferase as proxy for ATP content using the ENLITEN® ATP Assay System Bioluminescence Detection Kit (Promega) according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... phosphorothioated and phosphorylated/phosphorothioated forward primers as well as a phosphorylated reverse primer were done in qPCR reaction mixtures including 1X reaction mixture of GoTaq 2-Step RT-qPCR kit (Promega, USA), 400 nM forward and reverse primers and in final volume of 20 μL ...
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bioRxiv - Molecular Biology 2021Quote: ... Either GoTaq 1-Step RT-qPCR System (Promega) was used or reverse transcription for mRNAs was performed using Superscript IV RT (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: RNA was reverse transcribed using AMV RT (Promega) with random 9-mer primers ...
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bioRxiv - Microbiology 2020Quote: ... RT was primed with oligo dT(15) (Promega) and held for 1 h at 42 °C ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5 μL of RT (4U, Promega®), followed by an incubation at 42°C for 60 minutes ...
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bioRxiv - Microbiology 2022Quote: ... and 0.25 μL of RT (4U, Promega®), followed by an incubation at 42°C for 60 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by reverse transcription with AMV RT (Promega) and RT primer ...
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bioRxiv - Genomics 2020Quote: ... 5 x RT Improm II reaction buffer (Promega), 50 ng hexanucleotides ...
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bioRxiv - Molecular Biology 2022Quote: ... GoTaq® qPCR and RT-qPCR Systems (Promega), and reverse transcription system (Promega) ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR using GoTaq qPCR Master Mix (Promega) was performed on a QuantStudio7 Flex (Applied Biosystems) ...
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bioRxiv - Biochemistry 2023Quote: ... and GoTaq 1-Step RT-qPCR System (Promega) were used for qPCR assays ...
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bioRxiv - Pathology 2023Quote: ... Complementary DNA was synthesized using MMLV-RT (Promega). The sequence of the primers for the Hamp ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was performed using AMV RT (Promega) in the presence of RNasin (Promega) ...
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bioRxiv - Evolutionary Biology 2023Quote: The 419 bp sequence of 3’UTR containing predicted target sites for miR-252 in CcTRPM was cloned into the pmirGLO vector (Promega, Wisconsin, USA) downstream of the luciferase gene to construct the recombinant plasmid of CcTRPM-3’UTR-pmirGLO using the pEASY®-Basic Seamless Cloning and Assembly Kit ...
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bioRxiv - Biochemistry 2021Quote: ... Amplified PCR products were visualized by blue light illumination and the 504/505 bp bands were excised and the amplified PCR product purified using the Wizard SV Gel and PCR Clean-Up kit (Promega, Madison, WI, USA). Extracted DNA was Sanger sequenced at the University of Michigan Advanced Genomics Core and analyzed for presence of the single base pair deletion using DNAStar LaserGene SeqMan Pro 15 ...
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bioRxiv - Molecular Biology 2023Quote: The DNA was extracted using the Promega PCR Cleaning Kit (Wizard® SV Gel and PCR Clean-Up System, Promega Cat.-No. A9285) as indicated in the manual ...
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bioRxiv - Genetics 2021Quote: ... Development and stop solutions were added followed by detection of absorbance at 450 on a Luminometer: GloMax-Multi Detection System (Promega, USA).
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bioRxiv - Microbiology 2022Quote: ... RNA samples and the negative extraction controls were screened in duplicate to investigate the presence of YFV RNA by RT-qPCR (GoTaq Probe 1-Step RT-qPCR System-Promega, USA), with primers and probes targeting the 5’-noncoding regions of the YFV genome (39) ...
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bioRxiv - Evolutionary Biology 2023Quote: Target gene and AAEL002401(reference gene) expression was quantified by RT-qPCR using FAM- and HEX -labelled GoTaq® Probe qPCR and RT-qPCR assay (Promega), TaqMan universal PCR master mix (Promega ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Levels of reporter mRNAs in eggs were measured 6 hrs post-injection by RT-qPCR using the GoTaq 2-Step RT-qPCR system as per manufacturer’s instructions (Promega, Madison, WI) with random primers for reverse transcription and oligos listed in Table 1 for the qPCR step.
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified with Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol and directly sequenced using 10F primer to identify individual bacteria isolates at the genus level ...
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bioRxiv - Plant Biology 2021Quote: ... Probes from PCR products were radiolabeled using the Prime-a-Gene kit (#U1100, Promega) in the presence of [α-32P]-dCTP (Hartmann Analytic ...
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bioRxiv - Developmental Biology 2019Quote: ... Reverse transcription-PCR was performed using a cDNA synthesis kit (Promega, Madison, WI, USA) and 2 μg of total RNA according to the manufacturer's protocol ...
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bioRxiv - Developmental Biology 2019Quote: PCR amplification was performed using GoTaq G2 Hot Start Green Master Mix kit (Promega) in a 25 µL standard reaction mix and the following program ...
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bioRxiv - Molecular Biology 2020Quote: ... All PCR reactions were performed using GoTaq® G2 DNA polymerase kit from Promega. All PCR products were examined using the QIAxcel® capillary electrophoresis system 100 (Qiagen).
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bioRxiv - Genomics 2022Quote: ... products were gel purified using the Wizard SV Gel and PCR Cleanup kit (Promega) and ligated into pDONR221-b1b4 (Invitrogen ...
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bioRxiv - Evolutionary Biology 2022Quote: ... amplicons were purified with a WizardTM PCR Clean Up kit (Promega, Fitchburg, WI, USA). PCR products were sequenced using the BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: The toxicity of PX was investigated on monolayers of BLEC seeded on 96 well plates or on inserts luminal side in co-culture experiments using the commercially available Cytotoxicity Detection Kit (Promega, United States). An aliquot of 25 μl medium was taken to quantify the lactate dehydrogenase (LDH ...
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bioRxiv - Cell Biology 2021Quote: TdT-mediated dUTP nick-end labeling (TUNEL) or EdU assays were performed by using in situ cell death detection kit (TUNEL, Promega Corporation, G3250) or EdU Click-iT® Assay Kit obtained from Thermo Fisher (C10639) ...
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bioRxiv - Physiology 2020Quote: ... The transferase dUTP Nick End Labeling (TUNEL) assay was performed on cryosections of rat eyes using the TUNEL apoptosis detection kit (DeadEnd Fluorometric TUNEL System; Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: The ATP quantification was performed using ENLITEN® ATP Assay System Bioluminiscence Detection Kit from Promega (protocol by (Yang et al., 2015)) ...
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bioRxiv - Immunology 2023Quote: DNA in the HHMX-stimulated supernatants of THP1 dual reporter cells was quantified using the QuantiFluor dsDNA Detection Kit from Promega (catalog # E2670) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... The partial frame of circ_0005962 containing the binding site or mutant binding site (wild-type or mutant-type) with miR-382-5p was amplified and cloned into the downstream of pGL4 vector (Promega, Madison, WI, USA) to generate circ_0005962-wt and circ_0005962-mut fusion plasmids ...
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bioRxiv - Cell Biology 2021Quote: ... and 1-3μl aliquots were taken for reverse transcription (RT) using the Moloney murine leukemia virus RT RNase H (−) (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: The quantification of viral RNA was carried out in one-step RT-qPCR using 3 µl of the eluted RNA with the GoTaq® Probe One-step RT-qPCR System (Promega, Poland). This procedure employed in-house primers and a probe specifically tailored for the HCoV-229E N sequence ...
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bioRxiv - Microbiology 2020Quote: ... using a GloMAX®-Multi Detection system (Promega, UK). 50 µL reagent added at a speed of 200 µl/s followed by mixing and 2 s delay ...
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bioRxiv - Microbiology 2022Quote: ... and read on a GloMax multi-detection system (Promega).
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bioRxiv - Bioengineering 2021Quote: ... for radiography detection or with untagged Methionine (Promega #L118A) and Green Lysine (FluoroTect™ GreenLys ...
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Core Microbiota Drive the Prevalence of Extracellular Antibiotic Resistome in the Water CompartmentsbioRxiv - Microbiology 2022Quote: ... Detection and quantification using QuantiFluor ™ -ST (Promega, USA). The raw eDNA samples and PCR products were checked by agarose gel electrophoresis (Fig ...
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bioRxiv - Neuroscience 2019Quote: ... ADP detection system (ADP-Glo) was purchased from Promega. Inhibitors were synthesized as described by Boyd et al [18] ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 100 μl ROS-Glo detection reagent (Promega, Southampton, UK) was added per well ...
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bioRxiv - Molecular Biology 2023Quote: ... 50ul of NAD/NADH-Glo™ Detection Reagent (Promega) was added to each well ...
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bioRxiv - Plant Biology 2023Quote: ... and quantified with a GloMax-Multi Detection System (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... the Nano-Glo HiBiT Lytic Detection Assay (N3040, Promega) was performed according to the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2023Quote: ... and the GloMax®-Multi Detection luminometer System (Promega). The experiments were carried out in three biological replicates for the Z-Cre vs ZZ-Cre ZIKV replicase comparison experiment and once for the ZZ-Cre NIEV ...
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bioRxiv - Immunology 2023Quote: ... the luciferase substrate was added for chemiluminescence detection (Promega; Luciferase Assay system ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μL of ONE-glo luciferase detection reagent (Promega) was dispensed per well and luminance signal recorded with an Envision plate reader (Perkin Elmer).
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bioRxiv - Microbiology 2019Quote: ... cDNA was synthesized using M-MLV RT (H–) (Promega) and U3 antisense primer 5’-TGTGTGGTAGATCCACATCG-3’ ...