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Citations for Promega :
401 - 450 of 1120 citations for TRNA cytosine 5 Methyltransferase TRDMT1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... followed by the addition of 5 μL RQ1 RNase-Free DNase (Promega, Cat# M6101) and incubation at 37°C for 15 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... 2ul/5-10mg DW of Trypsin/Lys-C (Endoproteinase LysC) Mix (Promega, Cat #V5071) was added to samples and incubated with continuous agitation at 150 rpm for overnight at 37°C ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins eluted at pH 9.0 were further treated with 5 U of CIAP (Promega) and the buffer provided by the manufacturer (a negative control was prepared without phosphatase) ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were digested with chymotrypsin (5 ng/μL) at 25ºC overnight (Trypsin gold, Promega). Digestion was stopped by addition of 5% formic acid and peptides extracted twice with 70% acetonitrile and 5% formic acid (10 min sonication) ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were incubated at 25°C for 5 days before CellTiter-Glo assays (Promega) were performed ...
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bioRxiv - Systems Biology 2023Quote: ... 5 x 106 cells were electroporated with 1 μg of NanoLuc pNL3.1 (Promega, N1031) vector and 0.2 μg of control vector ...
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bioRxiv - Immunology 2023Quote: ... Luciferase Assay System (E1501) and Passive Lysis 5 × Buffer (E1941) were purchased from Promega. C1q recombinant protein (A400 ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 μm tissue scrolls were processed using the Maxwell RSC RNA FFPE instrument (Promega). RNA concentration and quality was determined using a TapeStation automated electrophoresis instrument (Agilent) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with 5-100 pM of JF549-HTL (Cat. No. GA1110, Promega) and 50 nM Hoechst 33342 for an hour in complete medium ...
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bioRxiv - Neuroscience 2023Quote: ... transferred to opaque 96-well plates containing 5 μM furimazine (NanoLuc Luciferase Assay, Promega), and bathed in 5-HT at various concentrations (ranging from 0.1 nM to 1 mM) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... for 30 minutes then with 5 units/1 L RQ1 RNase-free DNase (Promega) for 10 minutes at room temperature ...
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bioRxiv - Genomics 2024Quote: ... Trypsin digestion was done by adding 5 µL reductively methylated trypsin (Promega, Madison, USA) to each sample and incubating the samples overnight at 37°C ...
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bioRxiv - Microbiology 2019Quote: The following commercial antibodies and dilutions were used for immunofluorescence staining: rabbit polyclonal antibody to HaloTag (Promega; G9281; 1:500 dilution), mouse monoclonal antibody to lamin A/C (Cell Signaling ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1:2000) and the bound primary antibodies were detected by a HRP-conjugated goat anti-rabbit secondary antibody (W401B, Promega; 1:2000). Membranes were developed and visualized using the Pierce™ ECL immunoblotting substrate (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2022Quote: ... secondary antibody staining was performed for 1 h at room temperature using antirabbit HRP or anti-mouse HRP secondary antibodies (Promega,1:10,000) in blocking buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti–β-galactosidase monoclonal mouse antibody (Promega, 1:500) was co-incubated with anti-mCherry polyclonal rabbit antibody (Bio Vision ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibody (Promega) (1:10,000 ...
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bioRxiv - Cell Biology 2020Quote: ... Rabbit anti-HaloTag antibody (G9218) was purchased from Promega, and Donkey anti-rabbit IgG (minimal cross reaction to Bovine ...
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bioRxiv - Plant Biology 2021Quote: ... Anti-rabbit IgG horseradish peroxidase-linked antibody (Promega, USA) was used as the secondary antibody at 1:10,000–20,000 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... The immunoblots were probed with appropriate secondary antibodies (Promega) and visualised by chemiluminescence (Supersignal detection kit ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... Secondary antibody (donkey α-goat HRP, Promega, 1:10,000). For His-tagged proteins ...
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bioRxiv - Immunology 2021Quote: ... incubated with anti-IgY secondary antibody (Promega Madison, USA) for an hour ...
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bioRxiv - Molecular Biology 2023Quote: ... and anti- mouse IgG antibody (1:1,000, #S3721, Promega).
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bioRxiv - Biochemistry 2023Quote: ... and anti-mouse IgG alkaline phosphatase conjugated antibody (Promega). Band intensities were quantified by Image J ...
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bioRxiv - Bioengineering 2023Quote: ... followed by blotting with HRP-conjugated secondary antibodies (Promega). The protein bands were obtained with the ChemiDoc XRS+system (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... antibodies followed by anti-rabbit HRP conjugate (Promega, W4018). Protein loading was visualised by staining membranes with Ponceau S and protein signals were developed using the SuperSignal West Femto chemiluminescence kit (Pierce).
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bioRxiv - Molecular Biology 2023Quote: ... HRP conjugated secondary antibodies anti-mouse (Promega, PR-W4012) and anti-rabbit (Invitrogen 65-6120) ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies (Anti-Rabbit/Mouse IgG HRP Conjugate, Promega) were used at 1:5000 in 5% Blotting-Grade Blocker (BioRad).
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bioRxiv - Developmental Biology 2024Quote: ... and secondary antibodies (anti-mouse HRP conjugate (Promega, W4028) and anti-rabbit HRP conjugate (Promega ...
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bioRxiv - Genetics 2021Quote: ... then a 5 min final extension (72 °C)—using GoTaq Green Master Mix (M7123, Promega) and the pCFD6_seqfwd and pCFD6_seqrev primers (Table S1 ...
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bioRxiv - Molecular Biology 2020Quote: Cells were blocked by pipetting 50ul of 5% Bovine serum albumin (BSA) (Promega, Cat: W3841) directly onto slides ...
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bioRxiv - Immunology 2019Quote: ... RAC2 3’UTR-: 5’-TTGCGGCCAGCGGCCGCTCTCCAAACTTGAATCAATAAATTT-3’ and inserted into a dual luciferase psiCHECK2 backbone (Promega). RAC2 mutant 3’UTR constructs were generated using Infusion HD cloning kit (Clontech ...
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bioRxiv - Genetics 2021Quote: ... DNA ligation was performed using 5 μl HC T4 DNA ligase (Promega, 50-100 U) in 1X Ligation Buffer (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... the 5’-end of each mRNA was capped using the Ribo m7G Cap Analog (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... alkylated with iodoacetamid (final concentration of 5 mM) and digested overnight with trypsin (Promega V5113) in 1 M urea at an enzyme-to-substrate ratio of 1:40 ...
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bioRxiv - Plant Biology 2021Quote: ... each primer at 0.25 μM and 5 μL of 2X GoTaq qPCR Master Mix (Promega). The amplification program consisted of preincubation steps at 50°C for 2 min and 95°C for 10 min followed by 40 cycles of 95°C for 15 s and 60°C for 1 min ...
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bioRxiv - Microbiology 2021Quote: ... Samples were digested overnight at 37 °C with 5 µg sequencing-grade modified trypsin (Promega). The samples were then acidified with trifluoroacetic acid (TFA ...
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bioRxiv - Microbiology 2020Quote: ... Cells (5 × 106) were then lysed using 100 μl of RIPA lysis buffer (Promega, WI) supplemented with 1 μl of proteinase inhibitor and 10 μl of phenylmethylsulfonylfluoride ...
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bioRxiv - Molecular Biology 2022Quote: ... terminated by incubation on ice and diluted 1:5 in Glo Lysis Buffer (Promega # E2661). 25 μL of prepared Nano-Glo reagent (Promega # N1120 ...
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bioRxiv - Systems Biology 2019Quote: ... Half of the cDNA (5 μl) was amplified by PCR using Pfu Polymerase (Promega, M7745) with the cycling conditions (95°C for 2 min ...
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bioRxiv - Cancer Biology 2019Quote: ... before dilution with 120 μL 0.04 × ProteaseMax and 5 μg/μL sequencing grade trypsin (Promega) in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Survival was determined after 5 days of incubation using the Cell Titre-Blue assay (Promega). All cell lines were treated with 4 and 8 µM Erastin and 3 and 6 µM RSL3 ...
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bioRxiv - Plant Biology 2022Quote: ... DNA was incubated at 80°C for 5 min and mixed with FuGENE HD (Promega) in a ratio of 3:1 (FuGENE:DNA ...
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bioRxiv - Plant Biology 2022Quote: ... and 0.3 µl of Taq polymerase (5 u/µl) provided by Promega (Madison, Wisconsin, U.S.A.). Variations in MgCl2 (0.5–1 µl ...
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bioRxiv - Plant Biology 2023Quote: ... 5’RACE products were gel-purified and cloned in pGem®-T Easy vector (Promega). Independent clones were randomly chosen and sequenced by Sanger sequencing method.
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bioRxiv - Cancer Biology 2023Quote: ... resuspended in 400 μL 50 mM NH4HCO3 containing 5 μg modified sequencing grade trypsin (Promega), then transferred to a protein LoBind tube (Eppendorf ...
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bioRxiv - Biochemistry 2023Quote: ... with 25 μL of diluted reactions (diluted 1:5 in Glo Lysis Buffer; Promega # E2661) and incubated at room temperature for 5 min ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μg of the isolated recombinant bacmid and 5 μL Fugene HD transfection reagent (Promega) were diluted in 150 μL Sf-900™ III SFM ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... cDNA synthesis from 5 µg RNA was performed using the GoScriptTM Reverse Transcriptase Kit (Promega). Next ...
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bioRxiv - Cell Biology 2023Quote: ... Purified 5 μg of pAd/CMV/V5-DEST plasmid was transfected using Fugene HD (Promega) when the 293A cells were 80% confluent ...