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Citations for Promega :
401 - 450 of 8272 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... NanoBRET™ Nano-Glo® Substrate - furimazine (#N1663) and HaloTag® NanoBRET™ 618 Ligand (#G9801) were from Promega. Dimethylsulfoxide (DMSO ...
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bioRxiv - Molecular Biology 2023Quote: ... After incubation at 37 °C/5 % CO2 for 20 h 40 nl HaloTag® NanoBRET™ 618 Ligand (PROMEGA) was added to the cells using an Echo acoustic dispenser (Labcyte ...
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bioRxiv - Biochemistry 2023Quote: ... penicillin 100 U/ml and streptomycin 100 μg/ml) +/-HaloTag® NanoBRET™ 618 Ligand (0.5 μl/ml, Promega). 4 h later ...
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bioRxiv - Cell Biology 2023Quote: ... 4 hours post-transfection media was replaced with fresh complete DMEM containing JF646 HaloTag ligand (20 nM; GA112A; Promega) when required ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Target engagement and intracellular availability for VHL ligands were assessed using the NanoBRET TE Intracellular E3 ligase assay (Promega). HEK293 cells were transfected with the VHL-NanoLuc fusion constructs using FuGENE HD (Promega ...
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bioRxiv - Developmental Biology 2023Quote: ... HEK293 culture medium was replaced by imaging medium containing either Janelia Fluor 646 (JF646) ligand (10 nM; Promega, #GA1120) or OregonGreen ligand (50 nM ...
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bioRxiv - Biochemistry 2023Quote: Cell viability assays using CellTiter-Glo 2 (Promega) were performed according to the manufacturers’ instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... His-tag proteins have been purified by using MagneHis system (Promega) and DynaMag spin magnet (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2019Quote: ... expression and purification described in Supporting Information) with (experimental) and without (control) 100 nM HaloTag NanoBRET 618 fluorescent ligand (Promega) was combined with diacetylated or triacetylated histone peptide concentrations ranging from 10 nM to 100 µM in white ...
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bioRxiv - Cell Biology 2021Quote: ... Cells with MCP-Halo integration were isolated by FACS on a Sony SH800 flow cytometer after staining with JF646 HaloTag ligand (Promega). PB-PuroPonA-Kdm5b-MS2 plasmid and mPB PiggyBac transposon plasmid were then introduced to C2C12 myoblasts at 50% confluency in 6-well plates using Transit-LT1 transfection reagent according to manufacturer’s specifications ...
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bioRxiv - Cell Biology 2020Quote: DRGs neurons infected with lentivirus expressing Halo-SFPQ were grown in XonaChip microfluidic chambers and were labeled with tetramethyl Rhodamine (TMR) Halo Tag ligand (Promega) according to the manufacturer’s instructions with modifications ...
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bioRxiv - Cell Biology 2020Quote: ... Some cells at this stage were seeded onto coverslips and stable transfection of desired plasmids was confirmed by using specific fluorescent ligands to Halo-tag (TMR, Promega). The cells seeded in T75 flasks were allowed to grow for further 3-4 weeks in complete media with G418 replaced twice a week ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted protein was labeled with either 1.75 µM AlexaFluor 660 or 2.5 µM TMR HaloTag Ligands (Promega, G8471 and G8251) for 2 hr on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... A single camera was used to capture both Fab (AF488, Thermo-Fisher) and Halo-H2B stained with Halo-ligand (TMR, Promega). A high-speed filter wheel (HS-625 HSFW TTL ...
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bioRxiv - Molecular Biology 2022Quote: ... expressing PALLD and pNLF1-N [CMV Hygro] or pNLF1-C [CMV Hygro] vector (Promaga) expressing ANKRD1 were treated with 100 nM HaloTag NanoBRET 618 Ligand (Promega), whereafter signals were detected six hours later using a Synergy 4 instrument (BioTek) ...
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bioRxiv - Molecular Biology 2023Quote: ... penicillin (100 U/ml) and streptomycin (100 μg/ml) in the presence or absence of compounds and HaloTag NanoBRET 618 Ligand (Promega) for 4 h ...
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bioRxiv - Biophysics 2023Quote: 5 μM purified EGFP-YbbR-Lphn3 GAIN-HaloTag protein in HBS was mixed with 5.2 μM HaloTag PEG-Biotin Ligand (Promega, G8592) and incubated at room temperature for 2 hours to biotinylate the CTF of the GAIN construct ...
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bioRxiv - Cancer Biology 2022Quote: ... and 15 min prior to imaging 3 pM silicon rhodamine (SiR) HaLoTag ligand (kindly provided by K. Johnson, Heidelberg, Germany) were added according to the HaloTag staining protocol (Promega). Thereafter ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 5 × 104 transfected cells were replated into white 96-well tissue culture plates in the presence or absence of HaloTag NanoBRET 618 Ligand (Promega) and incubated overnight at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: Cells were reactivated or mock treated for 48 h and then labelled at least 30 minutes prior to harvesting with Halo ligand Janelia Fluor 646 (Promega) at a 1:1,000 dilution of 20 μM stock in DMSO ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 3 nM of either XeARPP19 or various forms of ClyARPP19 (stock solutions at 1 μg/μL) were incubated in the presence of 62.5 units of recombinant bovine PKA (Promega) and 1 mM γS-ATP in a final volume of 30 μL of PKA Buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... HiBiT-tagged proteins were detected using the Nano-Glo HiBiT blotting system (Promega) according to the manual ...
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bioRxiv - Cell Biology 2021Quote: ... Cell death was determined according to the activity of lactate dehydrogenase (LDH) in the culture supernatants using a CytoTox® Kit (Promega, USA) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... t-ERK1/2 (Promega V114A, 1/1000 dilution), p-AKT (Cell Signaling Technology ...
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bioRxiv - Biophysics 2022Quote: ... 2 μg trypsin (protein:enzyme ratio 50:1; Promega) were added and proteins were digested at 37 °C for 18 h ...
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bioRxiv - Neuroscience 2021Quote: ... after which D-luciferin-specific Quantilum Recombinant Luciferase (Promega) was added to a final concentration of 25 μg/mL ...
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bioRxiv - Plant Biology 2019Quote: ... and 20 units of recombinant Rnasin RNase inhibitor (Promega) in a final volume of 20 µl ...
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bioRxiv - Biochemistry 2019Quote: We also tested aggregation using Quantilum Recombinant Luciferase (Promega), L-malate dehydrogenase (MDH ...
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bioRxiv - Immunology 2022Quote: ... 200 U/mL Recombinant RNasin® Ribonuclease Inhibitor (Promega), 2 mM DTT) ...
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bioRxiv - Immunology 2022Quote: ... 200 U/ml Recombinant RNasin® Ribonuclease Inhibitor (Promega), 1 tablet cOmplete™ ...
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bioRxiv - Microbiology 2023Quote: ... A total of 5 U of recombinant RNasin (Promega) and 10 U of RQ1 RNase-free DNase (Promega ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5 µl Recombinant RNasin® Ribonuclease Inhibitor (Promega, N251A), 1 µl Reverse Transcriptase (Promega ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5 µl Recombinant RNasin® Ribonuclease Inhibitor (Promega, N251A), 1 µl Reverse Transcriptase (Promega ...
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bioRxiv - Microbiology 2023Quote: ... along with 40 units of Recombinant RNasin (Promega, USA). After confirmation of DNA free preparations using PCR ...
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bioRxiv - Biophysics 2024Quote: ... 80 U of Recombinant RNase inhibitor (Promega, Fitchburg, WI), 8 U of inorganic pyrophosphatase (Sigma-Aldrich ...
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bioRxiv - Biophysics 2024Quote: ... 800 U of Recombinant RNase inhibitor (Promega, Fitchburg, WI), 8 U of inorganic pyrophosphatase (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... T47D cells and MCF7 cells were transfected with empty or human LIP-containing pcDNA3.1 via Fugene HD (Promega) using the manufactures protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were lysed after 72 h (2+1 DIV for differentiations) using the Dual Luciferase Reporter kit (Promega, E1960) and luciferase activity was measured using a Victor3 (Perkin Elmer ...
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bioRxiv - Genomics 2022Quote: ... diluted to 1% in purified human genomic DNA (Promega, female, catalog No. G1521). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... containing 300 ng of the recombinant T/F LTR-pGL3 plasmid and 100 ng of recombinant control insert DNA-pTarget plasmid (Promega Corporation, Madison, WI) for the Tat minus transfection or 100 ng of recombinant HIV-1C consensus or autologous Tat-pTarget plasmid (obtained from the same sample from which the LTR was derived ...
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bioRxiv - Genomics 2020Quote: ... Cells that were transfected with the Nelfb-Halo repair template were incubated for 15 min with 500nM Halo TMR ligand (Promega G8251), washed 3 times with 2i media and further incubated for another 30 min before sorting for Texas Red ...
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bioRxiv - Cell Biology 2021Quote: ... conditions with HaloTag fusion protein expression were also incubated with 100nM of Janelia Fluor® 646 HaloTag® Ligand (Promega GA11220) for 30min prior to fixation ...
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bioRxiv - Cell Biology 2019Quote: ... non-fluorescent blocking ligand was washed out and cells were additionally washed three times with fresh DMEM medium and incubated DMEM medium containing Janelia Fluor 646 (JF646) HaloTag ligand (Promega, GA1121) in final labeling concentration 200 nM for indicated timepoints.
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bioRxiv - Cell Biology 2022Quote: For STED imaging proteins of interest were genetically fused to the HaloTag (Halo) and subsequently labelled with Janelia Fluor®646 HaloTag ligand (#GA1121, Promega) by incubating 200 nM ligand (from 200 μM stock in DMSO ...
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bioRxiv - Molecular Biology 2023Quote: ... A volume of cells equivalent to 1 mL at OD600 = 0.2 was centrifuged and re-suspended in 1ml fresh medium supplemented with JF549 (Janelia Fluor®HaloTag®Ligands, Promega) at a final concentration of 1 µM ...
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bioRxiv - Biophysics 2023Quote: ... each respective cell type was incubated at 37 °C with 500 pM of Janelia Fluor® 646 HaloTag Ligand (NSCs, ECs, or keratinocytes) (Cat. No. GA1120, Promega) or Janelia Fluor® 635 HaloTag Ligand (MNRs ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-m6A conjugated beads were incubated with purified mRNA with rotation at 4°C overnight in 300 µL MeRIP buffer with 1 µL RNase inhibitor (recombinant RNasin; Promega). 10% of the mRNA sample was saved as the input fraction ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were dissolved in 50 μL of 50mM TEAB and treated with 1 μL of recombinant PNGaseF (Promega, Madison, WI) overnight at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: RNA was isolated from pooled neurons that were stored at −80 in saline solution with 1% (v/v) Recombinant RNasin Ribonuclease Inhibitor (Promega). The RNA was extracted with the Quick-RNA micro prep kit (Zymo ...
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bioRxiv - Immunology 2020Quote: ... anti-m6 A conjugated beads were incubated with purified mRNA with rotation at 4C overnight in 300 mL MeRIP buffer with 1 mL RNase inhibitor (recombinant RNasin; Promega). 10% of the mRNA sample was saved as the input fraction ...