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Citations for Promega :
401 - 450 of 812 citations for KHK IN 2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... measured using a Biotek Synergy 2 multimode plate reader and the BacTitr-Glo Cell Viability assay (Promega). There was a linear relationship between concentration and luminescence (R2 =0.998) ...
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bioRxiv - Immunology 2023Quote: ... Tryptic digest was performed in 50 mM ammonium bicarbonate buffer with 2 μg sequencing-grade trypsin (Promega) at 37°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transfected with 2 ug of pQCXIN ERAAP-dsRed using Fugene HD transfection reagent (Promega, E2311) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 μg of the linearized DNA was transcribed in vitro using T7 RNA polymerase (Promega Corporation). The obtained RNA was then purified using GeneJET kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with 20 μl of 4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) (AqueousOne, Promega) at 37°C for 1-2 hours for MTS colorimetric cell viability assay ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM TCEP) was added to the sample followed by 40 µg of sequencing grade trypsin (Promega). Proteins were digested overnight rotating at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... 2-4μg bacmid was transfected into Sf9 cells in 6 well plates using Insect-XPRESS (Promega E2311). Cells were incubated at 2°C for 5 days and bacmid YFP expression assessed by Leica DM IL LED Fluo microscope using a green fluorescent protein filter cube ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.5 µg (HEK293A, HEK293T, HEK293AΔARRB1/B2) or 2 µg (HEK293AΔGs, HEKS293AΔGi) Glo-22F cAMP plasmid DNA (Promega) and 2 µg MOCK DNA (HEK293A ...
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bioRxiv - Genomics 2023Quote: ... the final selection was performed by 3% agarose electrophoresis (80 V, 2 h, 1 kB Promega ladder). The selected clones were preincubated in growth medium (15 ml ...
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bioRxiv - Biochemistry 2024Quote: ... Each sample was divided into 2 aliquots and digested with trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega) and AspN (Sequencing Grade ...
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bioRxiv - Cancer Biology 2021Quote: ... total RNA (2 µg) was reverse transcribed into cDNA using a cDNA synthesis kit (Promega, Madison, WI, USA). The RT-PCR was assessed using r-Taq plus Master Mix (Elpis Biotech ...
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bioRxiv - Cancer Biology 2021Quote: ... Dual luciferase assay was performed 2 days post transfection using the Nano-Glo Dual-Luciferase Reporter Assay (Promega) in a Synergy Neo2 microplate reader (Biotek) ...
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bioRxiv - Microbiology 2019Quote: DENV-2 viral RNA was extracted from sera using the Viral Total Nucleic Acid Kit (Promega, Madison, WI) on a Maxwell 48 RSC instrument (Promega ...
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bioRxiv - Molecular Biology 2021Quote: Cells were transfected with CRISPR–Cas9 and donor plasmids (Table 2) using FuGENE HD Transfection Reagent (Promega, #E2311) in a 6-well plate following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR fragments with lengths is above 500 bp to 2 kb were carried out with GoTaq enzyme (Promega) according to standard protocols ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μg of DNAse free RNA was used for cDNA synthesis using ImProm-II Reverse transcription system (Promega), as per the manufacture’s recommendation ...
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bioRxiv - Genomics 2019Quote: ... Alternatively, ¼ of an agarose plug was soaked in GET solution (3% 2-mercaptoethanol, 0.5x QuantiFluor® dye (Promega), 1X TBE ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were further diluted to a final urea concentration of 2 M and proteins digested with trypsin (Promega) (1/100 ...
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bioRxiv - Cell Biology 2021Quote: ... promoter fragments were cloned in front of the Renilla luciferase ORF (hRluc) into the psiCHECK-2 vector (Promega, Cat# C8021 ...
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bioRxiv - Plant Biology 2021Quote: ... 2 ug of RNA were reverse transcribed using a reverse transcription kit (GoScript™ Reverse Transcription System, Promega). The abundance of specific transcript was measured by probing 1 μL cDNA by quantitative real-time PCR in a total volume of 10 µl containg 5 μL SYBR-green master mix (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... PCR#2 product was ligated to pGEM-T easy vector or TOPO vector following the manufacturer’s instructions (Promega). After transformation in bacteria and plasmid amplification ...
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bioRxiv - Microbiology 2022Quote: ... virus was removed and cells were transfected with 2 µg of plasmid DNA pUCSP-A24Rcd using FuGeneHD (Promega) following manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... pH 8.0) with 2-5µg plasmids pre-digested by XcmI and 50 µg of herring sperm carrier DNA (Promega), then 700 µL of yeast transformation buffer (40% (w/v ...
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bioRxiv - Bioengineering 2022Quote: ... Uptake of 2-deoxyglucose by the adipocytes was measured using a Glucose Uptake-Glo™ Assay kit (Promega) and luminescence was measured according to the manufacturer’s instructions using a plate reader ...
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bioRxiv - Molecular Biology 2020Quote: ... The bacterial surface proteins with attached plasma proteins were released by limited proteolysis with 2 μG trypsin (Promega) /37 °C ...
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bioRxiv - Genetics 2022Quote: ... 2 µl of the extracted genome solution was used as a template for Genome PCR by GoTaq (Promega). The sequences of gw182 gene specific primer set designed around the CRISPR target site were 5’ -GAG TCC AAT TTG AGA AAC GGA GGT CA-3’ and 5’ - CTG ATC GTT TGC GCT TAA CTT CAT TAA TTC T-3’ ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: RNA was extracted after 2 days of culture with the ReliaPrep™ RNA Tissue Miniprep System (Promega, Z6111) following manufacturer’s protocol for non-fibrous tissue by adding RNA lysis buffer on pelleted cells ...
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bioRxiv - Genetics 2020Quote: ... cells were changed into GloSensor equilibration medium (10% fetal bovine serum and 2% GloSensor assay reagent (Promega E1290) in CO2-independent medium (Gibco 18045-088) ...
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bioRxiv - Biochemistry 2019Quote: ... In-column digest was performed by adding 25 µL of a solution containing 2 µg trypsin (Promega, V5111) in 50 mM TEAB pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: Cell viability was assayed with an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) colorimetric assay (Promega) per manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... cells were treated with 3 µM PP242 for 2 h and lysed with Passive Lysis Buffer (Promega, E194A). Luminescence was detected with a Dual- Luciferase Reporter Assay System (Promega ...
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bioRxiv - Immunology 2019Quote: ... NGP conditional medium was collected 2 days after transfection of NGP to 293T cells by Fugene HD (Promega). 10 ng/ml of LPS was mixed with conditional medium for 30 minutes and then treated to THP-1 cells.
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... containing 2μl of lysis buffer (1.9ml of 0.2% v/v Triton-X l00 v/v + 0.1ml of RNasin Plus RNase inhibitor (10,000 U/ml, Promega). Smart-seq2 protocol was performed as described in Picelli et al [43] ...
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bioRxiv - Genetics 2020Quote: ... The 10 µL Zn-finger PCR reaction was prepared containing of 2 µL of 5X reaction buffer (Promega), 0.8 µL of 2.0 mM MgCl2 ...
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bioRxiv - Microbiology 2021Quote: ... virus inoculum was removed and cells were then transfected with 2 µg of plasmid DNA using FuGeneHD (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: Calpain activity was measured in Caco-2 cell or organoids extracts by Calpain-Glo™ Protease Assay (Promega) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... genomic DNA was extracted from 2-wk old seedlings by using the Wizard Genomic DNA purification kit (Promega) and was subjected to PCR analysis by using a primer set consisting of a specific CaM35S promoter sequence and a gene-specific primer (Table S1) ...
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bioRxiv - Plant Biology 2022Quote: ... 2 μg of RNA were reverse transcribed using a reverse transcription kit (GoScript™ Reverse Transcription System, Promega). The abundance of specific transcript was measured by probing 1 μL cDNA by quantitative real-time PCR in a total volume of 10 μl containg 5 μL SYBR-green master mix (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... All PCR reactions were run using 2 µL of extracted DNA in GoTaq® PCR Master Mix (Promega) and the following cycling protocol ...
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bioRxiv - Microbiology 2023Quote: ... and 2 μg of the spike SARS2 (D614G)-pCAGGS (Medicines & Healthcare Products Regulatory Agency CFAR100985) using FugeneHD (Promega) transfection reagent at a ratio of 1:3 DNA:Fugene in optiMEM (Gibco) ...
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bioRxiv - Genomics 2023Quote: ... 2×10e4 cells were plated in 96-well plates and CellTiter-Glo® Luminescent Cell Viability Assay (Promega) was used to quantify cell viability 48h after drug or vehicle treatment using a Tecan Infinite M Plex plate reader (Tecan ...
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bioRxiv - Microbiology 2023Quote: ... beads were suspended in digestion buffer (Tris 50 mM pH 7.5, urea 2 M, 1 mM DTT and 5 µg.µl of trypsin (Promega)) for 3 min at 30°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested in 100 mM ammonium bicarbonate containing 2 μg of trypsin/Lys-C enzyme mix (Promega) at 37 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed with 2× phosphate-buffered saline and lysed with 75 µL of passive lysis buffer (Promega), and then subjected to luciferase assay ...
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bioRxiv - Cancer Biology 2022Quote: ... 20,000 to 50,000 cells were lysed in the transposase reaction mix (12.5 μl 2xTD buffer, 2 μl TDE1 [Illumina], 10.25 μl nuclease-free water, and 0.25 μl 1% digitonin [Promega]) for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg of Turbo DNase treated total RNA was incubated with or without 25 U RNase One (Promega) and 25 μg RNaseA (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The size of any positive IS2404 detection was assessed against 2 µL of 100 bp DNA Ladder (Promega) and run at 50 V for 1.5 hrs before being visualised with an EZ Gel Documentation System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... and their numbers were measured every 2–4 days using the CellTitre-Glo 3D cell viability assay (Promega), as described by the manufacturer ...