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Citations for Promega :
401 - 450 of 5909 citations for Human Lymphoid Enhancer Binding Factor 1 LEF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: pHalo-PPARγ2 expresses human PPARγ isoform 2 fused to HaloTag in the N-terminus under a CMVd1 promoter (Promega ORF clone #FHC08305). PPARγ2 mutants were generated by nucleotide substitution using the QuikChange II XL Site Directed Mutagenesis Kit (Stratagene ...
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bioRxiv - Cell Biology 2023Quote: ... the corresponding promoter regions of the human KLF2 and KLF4 genes were amplified using human genomic DNA as a template and cloned into the BglII site of the GL4 basic vector (Promega, Fitchburg, WI). Point mutants of the vectors were constructed by site-directed mutagenesis using a QuikChange Site-Directed Mutagenesis kit (Stratagene ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 24-48hrs in the blocking solution containing chicken anti-human NT-4/5 antibody (10 mg/ ml, Promega, Madison, WI) or rabbit anti-human NT-4/5 (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... qPCR targeting the β-globin gene was performed for each sample and evaluated alongside a β-globin calibration curve (human gDNA, cat# G1521, Promega).
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bioRxiv - Cell Biology 2019Quote: ... diluted 1/500 in PBS-BSA 1% and 0.4 U.mL-1 RNAsin (Promega). We visualized and quantified 5-FUrd incorporation by using high content imaging device (OPERETTA ...
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bioRxiv - Genomics 2021Quote: ... was extracted from tissue samples using a commercial kit (Wizard R Genomic DNA Purification Kit, Promega) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... pGEM®-T kit from Promega were used ...
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bioRxiv - Immunology 2019Quote: ... using a luciferase assay kit (Promega). Results were normalized to β-galactosidase activity (Galactostar ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: ... with the TNT kit (Promega: L1770). Each substrate was amplified with primers by PCR to allow T7-dependent transcription of the PCR product or transcribed directly if plasmids contained a T7 promoter ...
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bioRxiv - Microbiology 2019Quote: A Luciferase Assay System kit (Promega) was used ...
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bioRxiv - Plant Biology 2019Quote: The Reverse Transcription System Kit (Promega) was used to synthesize a cDNA library following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... or Luciferase Assay System kit (Promega) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... the PureYield™ MidiPrep Kit (ProMega) and NucleoBond Xtra™ Midi/Maxi Kit (Macherey & Nagel ...
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bioRxiv - Genetics 2021Quote: ... using the Renilla Glo Kit (Promega) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... the Glutamate Glo Assay Kit (Promega) was used ...
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bioRxiv - Microbiology 2021Quote: ... the Glucose Uptake-Glo kit (Promega) was used ...
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bioRxiv - Microbiology 2022Quote: ... The T7 RiboMax express kit (Promega) was used for the in vitro production of 16S rRNA under standard conditions.
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bioRxiv - Molecular Biology 2019Quote: ... using QuantiFluor ONE dsDNA kit (Promega) and library size was checked on the Bioanalyzer (Agilent Technologies ...
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bioRxiv - Immunology 2019Quote: ... using a luciferase assay kit (Promega). Results were normalized to β-galactosidase or Renilla activity (Galactostar ...
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bioRxiv - Molecular Biology 2020Quote: ... using the dual luciferase kit (Promega). Experiments were done at least in triplicate ...
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bioRxiv - Biochemistry 2020Quote: The “ADP-Glo” kit (Promega, Wisconsin) was used to measure hydrolysis of ATP to ADP and inorganic phosphate by ATPase ...
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bioRxiv - Bioengineering 2020Quote: ... Steady-Go luciferase assay kit (Promega) was used to measure the expression of luciferase in cells ...
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bioRxiv - Molecular Biology 2021Quote: ... a kit (Promega, Fitchburg, WI, USA) was used to perform TUNEL staining in accordance with manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: ... using GoTaq qPCR kit (Promega A6001). Plates were sealed using a microseal ‘B’ Adhesive Seal (Bio-Rad MSB1001 ...
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bioRxiv - Biochemistry 2020Quote: ... Using PureYield Plasmid Midiprep kit (Promega), DNA preparations of selected clones were subjected to sequencing at Eurofins Genomics Canada.
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bioRxiv - Cell Biology 2021Quote: ... with a HiBiT assay kit (Promega).
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bioRxiv - Microbiology 2021Quote: ... The Luciferase Assay System kit (Promega) was used to measure the luciferase activity in sporozoite samples following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and NADP+/NADPH-Glo kits (Promega), respectively ...
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bioRxiv - Evolutionary Biology 2022Quote: ... or Wizard PCR purification kit (Promega), and then directly sequenced using the same and internal primers on an ABI 3130xl sequencer.
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bioRxiv - Molecular Biology 2022Quote: ... GoScript Reverse Transcription Kit (Promega, #A5003) was used as per manufacturer instructions with the following modifications ...
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bioRxiv - Microbiology 2023Quote: One step RT-PCR kit (Promega) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... using SIRT-Glo Assay kit (Promega), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... as per the kit’s instructions (Promega), 75,000 mouse Fcγ receptor IV (mFcγRIV)-expressing Jurkat effector cells were added to each well to engage with the Fc region of M2e serum antibodies bound to native M2e expressed on the surface of the infected cells ...
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bioRxiv - Bioengineering 2023Quote: The NanoGlo luciferase assay kit (Promega) was used to determine the amount of functional Nanoluc protein in lysed biological samples by supplying the enzyme with substrate and immediately measuring the resulting luminescence ...
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bioRxiv - Cell Biology 2023Quote: ... Dual-Luciferase Assay Kit (E2920, Promega) was used to measure the dual-luciferase activity according to the manufacturer’s instruction.
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bioRxiv - Plant Biology 2023Quote: ... RNA of roots and nodule primordia was extracted using a TRIzol (Invitrogen)-adapted ReliaPrep RNA extraction kit (Promega), as previously described (53) ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was extracted using a TRIzol (Invitrogen)-adapted ReliaPrep RNA extraction kit (Promega) [39] ...
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bioRxiv - Developmental Biology 2023Quote: ... and the Reverse transcription kit (Promega) was used to make cDNA following manufacturer indications ...
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bioRxiv - Immunology 2024Quote: ... The dual luciferase assay kit (Promega) was used to determine the luciferase activity ...
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bioRxiv - Cancer Biology 2021Quote: ... short tandem repeat analysis verified the identity of mouse (Bioassay Methods Group, National Institute of Standards and Technology) and human (Promega GenePrint 10 System) cells.
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bioRxiv - Microbiology 2019Quote: ... These stock solutions were then diluted to the indicated concentrations in sequencing-grade water and 10 ng commercial human DNA (Promega Corp cat#G3041) was added to all samples ...
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bioRxiv - Synthetic Biology 2022Quote: Cytotoxicity assay was performed on HCT116 human colon cells (ATCC, #CCL-247™) using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, #G3580) which is a colorimetric method based on MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-tetrazolium ...
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Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2023Quote: HEK293 cells growing at 70% confluency in a 10 cm dish were transfected with wild-type human NOPR or NOPLight (3 µg DNA) and GloSensor-20F (Promega, 2.5 µg DNA) using 12 µL Lipofectamine 2000 (Thermo Fisher ...
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bioRxiv - Systems Biology 2023Quote: ... Peptide loading was assessed by comparing the intensity of the total ion current to 200 ng of a human K562 whole cell lysate standard (Promega, Madison, Wisconsin, USA) prepared at 0.2 μg/μL.
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bioRxiv - Immunology 2021Quote: ... A 1:1 dilution of Steady Glo (Promega) and Lysis Buffer were added to the cells and incubated at RT for 15 mins ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-1-gal (1:1000, Promega Z378B), mouse anti-1-gal (1:1000 ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... 1 mM CaCl2 with 1 µg trypsin (Promega). Digested proteins were acidified with TFA to pH < 2 and RapiGest SF was precipitated out ...
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bioRxiv - Biochemistry 2020Quote: ... GDP detection kit was from ProFoldin (MA, USA) and ADP-Glo™ Kinase Assay kit from Promega Corporation ...
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bioRxiv - Genetics 2022Quote: Genomic DNA was isolated from blood samples using commercial kit (Wizard® Genomic DNA Purification Kit; Promega), following the fabricant protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Standard curves for each primer set were generated using serially diluted plasmid (for gAAV) or Human and Mouse Genomic DNA (for host) (Promega G1521 and G3091, respectively) and used for quantification ...