Labshake search
Citations for Promega :
401 - 450 of 1558 citations for Human KIRREL shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... falciparum 3D7 DNA with commercially available human DNA (Promega, G304A) to a total amount of 400ng DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... 10ng/mL of recombinant human FGF (Promega Corporation, Madison, WI), and 1µM dexamethasone (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... transfer plasmid was co-transfected with GAG/POL and VSVG plasmids into HEK293T or 293FT cells using FuGENE HD (Promega, 3.33:1 FuGENE:DNA) and the medium was replaced 6–8 h after transfection ...
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bioRxiv - Immunology 2022Quote: The whole-genome sgRNA library plasmid (GeCKOv2, a gift from David Huang, University of Melbourne) plus packaging plasmids using FuGENE6 (Promega, cat. no. E2691) were transfected into low-passage HEK293T cells at 80% confluency in 15 cm tissue culture dishes ...
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bioRxiv - Molecular Biology 2023Quote: ... containing 300 ng of the recombinant T/F LTR-pGL3 plasmid and 100 ng of recombinant control insert DNA-pTarget plasmid (Promega Corporation, Madison, WI) for the Tat minus transfection or 100 ng of recombinant HIV-1C consensus or autologous Tat-pTarget plasmid (obtained from the same sample from which the LTR was derived ...
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bioRxiv - Cell Biology 2024Quote: ... BP1425-500) at 37 °C and the plasmid was isolated from single colonies using the PureYield Plasmid Midiprep kit (Promega, Cat no. A2495), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Transient transfection of plasmids was performed with Fugene HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and the expression plasmid of interest using Fugene HD (Promega). FLuc and RLuc activity were quantified using the Dual Luciferase Assay Kit (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... reporter plasmids (500 ng/ml) were transfected using FugeneHD (Promega). 24h after transfection luciferase levels determined using Promega dual luciferase assay and GloMax plate reader (Promega).
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bioRxiv - Cancer Biology 2019Quote: ... and pRL-CMV (a Renilla luciferase plasmid, Promega, Madison, WI) plasmids were transfected into HEK293T cells (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... and colonies were selected for plasmid purification (PureYield protocol, Promega). PCR products were then sequenced (Eurofins Genomics ...
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bioRxiv - Cell Biology 2020Quote: ... The pRLCMV reporter plasmid was obtained from (Promega, Madison, USA). The pmRFP-C1 and pEGFP-Rab7 plasmid were obtained from Dr Andrew Gilmore (Faculty of Life Sciences ...
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bioRxiv - Molecular Biology 2019Quote: ... the Renilla and Firefly luciferase genes in psiCheck2 plasmid (Promega) were first replaced by constructs with synonymous mutations in putative m6A sites (obtained as IDT gBlocks) ...
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bioRxiv - Cancer Biology 2019Quote: ... Plasmids were forward transfected into cells using FuGENE 6 (Promega). BEZ-235 ...
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bioRxiv - Cell Biology 2019Quote: ... coli and purified with the PureYield Plasmid Miniprep System (Promega). The plasmids were then transfected into HEK293T cells using jetPRIME transfection reagent (Polyplus Transfection ...
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bioRxiv - Cell Biology 2019Quote: ... Where indicated cells were transfected with plasmids using FugeneHD (Promega). C10 (del ΔH ...
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bioRxiv - Biophysics 2019Quote: ... expression plasmids were supplemented with empty vector DNA (pSI; Promega) to maintain the same ZP subunit/total DNA ratio.
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bioRxiv - Cell Biology 2019Quote: ... Plasmid transfections were performed with FuGENE 6 (Promega, cat#E2691) according to manufacturer instructions ...
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bioRxiv - Genomics 2019Quote: ... splice junction amplicons were cloned in pGEM-T plasmid (Promega) and subjected to Sanger sequencing ...
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bioRxiv - Neuroscience 2020Quote: ... IgSF8-EGFP and Tenascin-R-EGFP plasmids using Fugene6 (Promega). Twenty-four hours after transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Large scale plasmid generation was performed using Maxiprep columns (Promega), with purified plasmid used as input for lentiviral packaging with second generation packaging plasmids psPAX2 and pMD2.G (Cellecta) ...
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bioRxiv - Genomics 2021Quote: ... Transfection of plasmid (1μg) was done with FuGene HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Plasmid and siRNA transfections were done with Fugene 6 (Promega) and Lipofectamine RNAiMAX (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: The pGL3 reporter plasmid containing the SV40 early promoter (Promega) was used for all luciferase reporter assays along with an internal control Renilla plasmid (Promega) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The plasmid for luciferase (pBEST-Luc) was obtained from Promega. DNA for cell-free reactions was prepared from overnight bacterial cultures using Maxiprep kits (Macherey-Nagel).
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bioRxiv - Cell Biology 2022Quote: ... Plasmid transfection into RPE1 cells was performed using ViaFect (Promega) according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2020Quote: ... Nluc was amplified from the pNL1.1 plasmid (Promega, Madison, WI) with primers to ensure in-frame Nluc expression as well as containing 25 bp overlap to the insertion site to allow cloning by Gibson Assembly ...
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bioRxiv - Microbiology 2019Quote: ... together with pRL-CMV reference plasmid (0.03 µg/well; Promega). Cells were simultaneously co-transfected with 0.3 µg/well of the empty pCI-neo-3xFLAG expression vector or encoding viral proteins as specified ...
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bioRxiv - Microbiology 2019Quote: ... Amplified plasmid was then phosphorylated using T4 polynucleotide kinase (Promega) and self-ligated (T4 DNA ligase ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The sequence was subcloned into the pCMVTNT expression plasmid (Promega). Other plasmids used in this study express X ...
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bioRxiv - Immunology 2021Quote: ... cells were transfected with plasmids of interest using Fugene (Promega) as per the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2019Quote: ... qPCR products were cloned into pGEM®-T plasmid (Promega) and transformed into TOP10 chemically competent E ...
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bioRxiv - Microbiology 2020Quote: ... as carrier DNA and a luciferase-encoding plasmid (pGL4.26, Promega). qPCR was then performed to detect viral gene ORF26 and luciferase from the carrier plasmid using GoTaq polymerase (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293FT cells were transfected with the plasmid using FuGENE6 (Promega). After 24 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... 100 ng pGL3-Basic luciferase reporter plasmid (Promega, Cat. #E1751) with target sequence inserts were cotransfected into HEK293T and MCF7 cell lines with 200 ng of Control/YAP1-S127A construct and 10 ng of Renilla luciferase pRL-TK plasmid (Promega ...
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bioRxiv - Genetics 2020Quote: ... and 100ng of Renilla luciferase control plasmid (Promega; Accession # AF025848) was mixed with 3μL of BioT transfection reagent (Bioland Scientific LLC ...
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bioRxiv - Cell Biology 2020Quote: Plasmid pGL4.32[luc2P/NF-κB-RE/Hygro] (cat# E8491, Promega) was used as template DNA in a PCR for obtaining the minimal promoter Luc2P together with the NF-κB response elements (NF-κB-RE) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were transfected with psiCHECK-2 plasmids (Promega, Madison, USA) containing two luciferase genes ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid transfections were done using FuGENE HD (Promega, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.3 µg renilla luciferase control plasmid pRL-CMV (Promega) using lipofectamine 3000 ...
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bioRxiv - Biochemistry 2023Quote: ... After overnight attachment the dual reporter plasmids (pGL4.36, pGL4.74 (Promega)) ...
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bioRxiv - Microbiology 2023Quote: ... and isolated with PureYield Plasmid Miniprep System (Promega, Cat# A1222). Sequences of all plasmids were verified using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems ...
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bioRxiv - Genetics 2023Quote: ... The PCR product was cloned into pGEM-T plasmid (Promega) and sequenced ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant plasmids were confirmed by PCR (GoTaq DNA Polymerase, Promega) and Sanger sequencing (Azenta) ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 ng of Renilla luciferase expression plasmid (pRL-TK) (Promega) was co-transfected for transfection efficiency normalization (500 ng of DNA total was added per transfection) ...
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bioRxiv - Genetics 2023Quote: ... and the PureYield® Plasmid Midiprep Protocol System (Promega, USA). Verification of successful cloning was done by sequencing (Eurofins ...
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bioRxiv - Plant Biology 2023Quote: ... coli using PureYield™ Plasmid Miniprep (Promega, Madison, WI, USA), verified using Sanger sequencing (Laboratory of Biotechnology & Bioanalysis ...
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bioRxiv - Neuroscience 2023Quote: PCR reactions were ligated to pGEM T-Easy plasmid (Promega). Closed inserts were Sanger sequenced by the UChicago Comprehensive Cancer Center DNA Sequencing Facility ...
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bioRxiv - Microbiology 2023Quote: ... 200 ng of the IFN-β reporter plasmid (Promega, USA) and 4 ng of Renilla luciferase plasmid ...
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bioRxiv - Cancer Biology 2023Quote: ... and 0.3 ug renilla luciferase control plasmid pRL-CMV (Promega) on an Amaxa Nucleofector 2b (Lonza) ...