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Citations for Promega :
401 - 450 of 4877 citations for Human Gap Junction Alpha 8 Protein CX50 GJA8 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... To quantify the activity of reports groups of 5 embryos were collected 8 hours post-injection in triplicates and lysed in 100 mL of lysis buffer (Promega). Reporter expression was quantified with the Nano-Glo Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Biophysics 2024Quote: ... 8 μl of the terminated reaction mixture were then applied to a streptavidin biotin capture membrane (SAM2, Promega, Cat.# V2861) and further treated following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... In solution digests were performed overnight at pH 8 and 37 °C with mass spectrometry grade trypsin (Promega, Madison, WI) at a 1:33 enzyme-to-protein ratio and quenched with formic acid (0.15%) ...
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bioRxiv - Bioengineering 2023Quote: ... The AAV production media were collected 72 h and 120 h post transfection and concentrated using a PEG-precipitation method with 8% PEG-8000 (wt/vol; Cat# V3011, Promega). The harvested cell pellets were re-suspended and lysed through sonication ...
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bioRxiv - Neuroscience 2022Quote: ... the absorbance was measured at 450 nm (CCK-8) or 560 nm (MTT) using a microplate reader (GloMax explorer multimode, Promega).
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bioRxiv - Biochemistry 2022Quote: ... Luciferase expression was measured at 8 hr (for translation) and 72 hr (for replication) using the Renilla Luciferase Assay System (Promega). Renilla luciferase luminescence was measured using a Clariostar Microplate Reader (BMG Labtech ...
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bioRxiv - Cell Biology 2022Quote: ... and the -1680 bp to +8 bp and -1525/-1495 element mutant were constructed in the pGL3-Basic luciferase reporter vector (Promega) using standard molecular biology techniques ...
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bioRxiv - Microbiology 2023Quote: ... 8 × 105 HEK293T cells/well were seeded into 6-well plates and transfected the next day with ViaFect (Promega, E4981), using either 4 μg of plasmid expressing the G protein or 2 μg of plasmid expressing HA together with 2 μg of plasmid expressing NA ...
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bioRxiv - Genomics 2024Quote: ... and a second antigen retrieval step at 37°C using proteinase K (20 μg/ml in TBS, pH 8, V3021, Promega), the slides were blocked as described above ...
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A resource for extracellular vesicles from activated CD4 T cells that relay pro-inflammatory signalsbioRxiv - Immunology 2024Quote: ... Samples were precipitated using 1:8 vol:vol dilution of each sample in 90% methanol in 100 mM Triethylamonium bicarbonate and digested with trypsin (Promega, #V1115) overnight at 37° C ...
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bioRxiv - Immunology 2024Quote: ... samples were precipitated using 1:8 vol:vol dilution of each sample in 90% methanol in 100 mM Triethylamonium bicarbonate and digested with trypsin (Promega, #V1115) overnight at 37° C ...
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bioRxiv - Microbiology 2024Quote: ... Viral titers were measured by infecting MDCK cells and measuring Nanoluciferase activity 8 h later with the Nano-Glo Luciferase Assay System (Promega)(52).
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bioRxiv - Cancer Biology 2024Quote: Total cellular DNA was examined by quantitative PCR optimized for detection of ∼8-12kb fragments as previously described (33) using GoTaq Long PCR polymerase (Promega), Evagreen (1:20 Biotium) ...
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bioRxiv - Synthetic Biology 2024Quote: ... encoding each aaRS was incubated at 30 °C for 8 h in a customized PURE system (version 1) containing fluorescently labeled lysyl-tRNA (FluoroTect GreenLys, Promega). After expression ...
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bioRxiv - Cell Biology 2024Quote: ... Rinsed beads were resuspended in 15 µL of 50 mM HEPES buffer (pH 8) supplemented with trypsin/LysC mix (Promega) at an enzyme-to-protein ratio of 1:25 (w/w ...
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bioRxiv - Cancer Biology 2024Quote: ... Thermolysin proteolysis was performed in 100uL of 50mM ABC/10mM KCl (pH=8) by adding Thermolysin (Promega, 1:50 ratio) to proteins and incubating at 70°C for 1 hour at 900 rpm prior to acidification to 1% FA ...
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bioRxiv - Cancer Biology 2022Quote: ... Each sample was then diluted 10-fold with 50 mM ammonium bicarbonate for digestion with 0.375 µg (for 15 µg protein samples) or 0.125 µg (for 5 µg protein samples) sequencing grade modified porcine trypsin (Promega, Madison, WI, USA). The samples were incubated at 45°C for 1 hour in a chilled microwave (CEM ...
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bioRxiv - Biochemistry 2021Quote: ... the sample was diluted 1:1 with 50 mM ammonium bicarbonate and proteins were digested with trypsin (protein to enzyme ratio 1:50, Promega, Mannheim, Germany) overnight at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... In-solution digestion was performed with LysC-Trypsin mix (1:100 enzyme: protein ratio) and trypsin (Promega; 1:50 enzyme: protein ratio). Peptides were desalted on C18 stage tips ...
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Berberine is a novel mitochondrial calcium uniporter (MCU) inhibitor that disrupts MCU-EMRE assemblybioRxiv - Cell Biology 2024Quote: ... biotin or biotin-labeled berberine (2 μg) was pre-incubated with either recombinant protein (2 μg) or in vitro transcribed/translated protein (Promega, Cat# L1170) for 8 hours at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... The proteins were digested with trypsin (Promega Corporation, USA) at an enzyme-to-substrate ratio of 1:50 (w/w ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 μg of protein were digested with trypsin (Promega) in two steps (2 h at 37 ºC with 1:40 trypsin:protein ratio ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then digested with Trypsin Gold (Promega) 1:100 at 37 °C overnight in 10 mM ammonium bicarbonate pH 8.0 and the resultant peptides were cleaned up with POROS 20 R2 (Applied Biosystens) ...
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bioRxiv - Bioengineering 2021Quote: ... Proteins were digested with sequencing grade modified trypsin (Promega) at an enzyme to substrate ratio of 1:50 overnight at RT ...
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bioRxiv - Bioengineering 2020Quote: ... Proteins were digested with sequencing grade modified trypsin (Promega) at an enzyme to substrate ratio of 1:50 overnight at RT ...
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bioRxiv - Cell Biology 2021Quote: ... with that of the Halo 7-tag protein (Promega), with the insertion of a 63-base linker (21 amino acids ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were digested overnight with LysC/Trypsin mix (Promega) and sequencing-grade modified Trypsin (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... with Broad Range Protein Molecular Weight Markers (V8491, Promega) used as molecular markers ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were harvested in passive lysis buffer 1x (Promega) at indicated times and
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bioRxiv - Plant Biology 2021Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega) and analyzed by nano LCMS/MS on a QExactive + mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were digested with trypsin (Promega, Madison, Wisconsin, USA) in 40 mM AmBic at 37°C for 18 h (enzyme to protein ratio (v/w ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were digested first by Lys- C (Promega, USA) at RT for 2 hours ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were trypsinized overnight (Sequencing Grade Modified Trypsin, Promega, cleaves C-terminal to lysine and arginine residues ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were digested on beads with Trypsin/LysC (Promega) overnight followed by zip-tip cleanup using C-18 Omix tips (Agilent) ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were digested on beads with Trypsin/LysC (Promega) overnight followed by zip-tip cleanup using C-18 Omix tips (Agilent) ...
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bioRxiv - Synthetic Biology 2021Quote: ... A standard curve of HiBiT Control Protein N3010 (Promega) was diluted in PLB+PI at concentration ranging from 0.01 to 2 nM ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were detected with ECL Western Blotting Substrate (Promega) or SuperSignal™ West Femto Maximum Sensitivity Substrate (ThermoFisher ...
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bioRxiv - Plant Biology 2020Quote: ... GST-Halo was used as a protein standard (Promega). Incubate 1 μL of reticulocyte extract before binding with beads and 2 μL of extract after the binding was used for each western on a precast gel (4–20% Criterion™ TGX™ Precast Midi Protein Gel ...
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bioRxiv - Plant Biology 2020Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega) and analysed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested with 300ng trypsin (Trypsin Gold, Promega) in 3µl 50mM ammonium bicarbonate followed by incubation at 37°C overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were digested with 0.25µg of Trypsin/LysC (Promega) at a 1:100 enzyme/substrate ratio overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega) and analyzed by nanoLC-MS/MS ...
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bioRxiv - Neuroscience 2023Quote: ... then with trypsin (Promega, 1:50 (protease to protein)) for 6 hours on a 37 °C shaker.
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bioRxiv - Microbiology 2023Quote: ... enriched proteins were eluted by adding 1µg trypsin (Promega) and incubation for 30min at 30°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were digested first by Lys-C (Promega, USA) at RT for 2 hours ...
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bioRxiv - Synthetic Biology 2023Quote: ... using the HiBiT Control Protein (20 µM, N3010, Promega) as a standard.
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bioRxiv - Molecular Biology 2022Quote: ... and proteins were in-gel digested with trypsin (Promega), five fractions per sample ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested using trypsin/ Lys-C mixture (Promega) and desalted on a 10 x 4.0 mm C18 cartridge (Restek ...
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bioRxiv - Cell Biology 2024Quote: ... containing trypsin (Promega; final 1/100 enzyme/protein ratio) and LysC (Wako ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were digested on-beads with Trypsin (Promega) with ratio enzyme:proteins 1:20 in 50 mM ammonium bicarbonate and performed overnight at 37°C ...