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Citations for Promega :
401 - 450 of 4380 citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... SEA (0.2 mg/ml) and soluble schistosomula antigens (0.2 mg/ml) was determined using the Caspase-Glo® 3/7 Assay System (Promega, Sydney, Australia) according to the manufacturers’ instructions.
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bioRxiv - Microbiology 2022Quote: ... washed three times (10 minutes each) with PBST before incubation for 1 h with α-Mouse IgG HRP Conjugate (Promega) secondary antibody (1:10 000 in PBST) ...
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bioRxiv - Cell Biology 2022Quote: ... two micrograms of RNA from each sample were reverse transcribed at 42 °C for 1 h followed by 95 °C for 10 min M-MLV Reverse Transcriptase (Promega) and oligo(T)18 primers (Takara) ...
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bioRxiv - Genomics 2020Quote: ... A double-stranded DNA adapter with a T-overhang was ligated for 1 h at 20°C and the resulting products were bead purified (ProNex, Promega), eluted and then split into two identical aliquots ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 μl of 10 mM ddTTP for the sequencing reaction and 1 μl of MMLV Reverse Transcriptase RNAse H minus (Promega) were finally added and the reverse transcription was performed at 35°C for 2 minutes ...
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bioRxiv - Systems Biology 2019Quote: ... The sample was digested for 16 h at 37C using a 1:50 (w/w) ratio of Sequencing Grade Modified Trypsin (Promega):protein in the gel ...
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bioRxiv - Biochemistry 2019Quote: ... The washed beads were resuspended in 200 µl of digestion buffer and incubated for 5 h with 1 µg of trypsin (Promega) at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... The messenger RNAs (mRNAs) contained in 1 μg of purified RNA extracts were reverse-transcribed using the reverse transcriptase RNase H minus (Promega) and oligo-d(T)15 ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of RNA was converted into cDNA with M-MLV RNase H–Point Mutant reverse transcriptase (Promega Corp., USA) and an anchored oligo dT21 primer (Metabion ...
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bioRxiv - Plant Biology 2020Quote: ... and then reverse-transcribed with a cox1 specific primer (Atcox1-1; table S1) using M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RNase H minus, point mutant; Promega). The RNA template was degraded by adding 1/5 volume of 1 M NaOH to the reaction mixture and incubating at room temperature for 10 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... The soluble fraction was then digested for 20 h at 37°C using a 1:50 ratio of trypsin (Promega) to extracted protein while gently shaking ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were diluted with ddH2O 1:5 and proteins were digested for 20 h at 37°C using trypsin (Trypsin Gold, Promega) at an enzyme/protein ratio of 1:50 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the proteins were alkylated with iodoacetamide (100 mM for 1 h at room temperature in a dark chamber) and digested with trypsin (Promega) diluted in ammonium bicarbonate (0.1 M for 24 h at 37 °C) ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were harvested 36 h after transfection by lysing in 100 μl of 1× reporter lysis buffer (Promega, Madison, WI). Lysates were pelleted ...
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bioRxiv - Biophysics 2021Quote: ... were plated into six-well tissue culture plates containing collagen-coated glass cover slips 12 to 24 h before being transfected with 1 μg of the indicated plasmid DNA using FuGENE 6 transfection reagent (Promega) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... 8 μg of RNA were treated for 1 h at 37°C with 12 units of RQ1 DNase (Promega®) followed by phenol-chloroform extraction and ethanol precipitation of RNA which was subsequently dissolved in water ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed once with PBS and lysed for at least 1 h with 40 μl 1x passive lysis buffer (Promega) at RT ...
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bioRxiv - Bioengineering 2023Quote: ... tumor fragments (200-300 mg) were lysed for 1 h on ice with agitation in 1X Reporter Cell Lysis Buffer (Promega) and then centrifuged at 14,000 x g for 15 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 1 h at 37 °C with fresh complete DMEM containing JFX646 HaloTag ligand (200 nM; GA112A; Promega), washed twice with PBS 1X and imaged on an Elyra 7 with Lattice SIM² microscope (Zeiss ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were diluted with seven volumes of 50 mM ammonium bicarbonate and incubated for 16 h at 37 °C with 400 ng of trypsin (ratio 1:50; “Trypsin Gold”, Promega).
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bioRxiv - Biochemistry 2024Quote: ... at a 1:75 enzyme to protein ratio for 6 h at 30 °C and then by trypsin (V5111, Promega) (1:50 enzyme to protein ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... Denatured proteins were enzymatically digested for 14 h at 37 °C with 1 µg of sequencing-grade Trypsin (V511A, Promega). After speed-vaccum drying ...
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bioRxiv - Cell Biology 2023Quote: The activity of the proteasome’s β5 sites was determined either by Succinyl(Suc)-LLVY-AMC (7-amido-4-methylcoumarin) fluorogenic substrate or by the Proteasome-Glo™ assay (Promega), a luciferase coupled assay ...
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bioRxiv - Cell Biology 2020Quote: ... Caspase3 / 7 Kit (Promega company).
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bioRxiv - Microbiology 2021Quote: ... diluted 1:2 with PBS and read on a GloMax Multi+ Detection System (Promega). CSV files were exported onto a USB flash drive for analysis.
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bioRxiv - Systems Biology 2020Quote: ... The transfections were performed with 1 μg DNA: 2 μl Fugene HD (Promega E2311) ratio ...
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bioRxiv - Microbiology 2022Quote: ... diluted 1:2 with PBS and read on a GloMax Multi+ Detection System (Promega).
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bioRxiv - Immunology 2022Quote: ... 1-2 µg RNA was reverse transcribed using AMV Reverse Transcriptase (Promega, Madison, WI). Primers were purchased from Integrated DNA Technologies (IDT ...
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bioRxiv - Cell Biology 2022Quote: ... and 1-2 µg of RNA was reverse transcribed into cDNA using GoScript (Promega).
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bioRxiv - Biophysics 2019Quote: ... cells were treated with the LRRK2 inhibitor MLi-2 (100 nM) for 2 hours and then labelled with the TMR ligand (3 μM)(Promega). After 15 minutes ...
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bioRxiv - Genomics 2021Quote: ... cells were transfected in a 3:1 ratio of FuGENE® HD Transfection Reagent (Promega cat # E2311) and plasmid DNA (pcDNA3.1-HMGB1P1-3xHA ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 µL of PBS containing 4 μg/mL Hoechst and 1/10000 CellTox Green Dye (Promega) were were dispensed per well 1 h prior to imaging at Cytation5 image cytometer or Opera Phenix (Perkin Elmer ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μL of the annealed oligos were ligated with 0.04 ng digested PspCAs13b crRNA backbone in 10 μL of T4 ligation buffer (3 h, RT) (Promega, M1801). All PspCas13b crRNA spacer sequences used in this study are listed in Supplementary File 1 ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were reduced by dithiothreitol and alkylated by iodoacetamide in the dark at room temperature, followed by in-solution digestion sequentially using lysyl endopeptidase (Lys-C, Wako) for 3 h and trypsin (Promega) overnight at room temperature as previously described (Paul et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’int-F and 24nt-6stp-R primers and 2) 3’KI: 24nt-6stp-F and 3’int-R using the GoTaq DNA polymerase mix (Promega, Madison, WI) and 200 nM of each primer ...
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bioRxiv - Molecular Biology 2023Quote: ... The released peptides were then transferred over to a new tube and a 4 h digest using 0.5 ug mass spectrometry-grade trypsin (V5111, Promega) at 37 C for 4 hours was conducted ...
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bioRxiv - Neuroscience 2024Quote: ... the medium was removed and substituted with OptiMEM media supplemented with 4 µM coelenterazine-H (Promega; Cat. No. S2001), and incubated for a period of 2 to 3 hours ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... membrane integrity (CytoTox-OneTM homogeneous membrane integrity assay kit: lactate dehydrogenase (LDH release) and caspase activity/ apoptosis (Caspase-Glo® 3/7 assay system kit) (all from Promega, WI, USA), as described previously17 (see Supplementary data for detailed description).
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bioRxiv - Bioengineering 2024Quote: ... 100 uL of the diluted lysate was added to each well along with an equal volume of Caspase Glo 3/7 assay reagent (Promega, Madison, Wisconsin, USA), according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2021Quote: ... was added to each well and incubated for 1 h at RT before fluorescence intensity was read on a Glomax® Explorer Fully Loaded (Promega) with emission wavelengths of 500-550 nm and an excitation wavelength of 475 nm and graphs were generated using GraphPad Prism (V.8.4.1) ...
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bioRxiv - Cell Biology 2023Quote: ... samples were resuspended in 50 µl of 50 mM ABC buffer and digested at 37°C for 16 h using 1 µg of trypsin (Trypsin Gold, Promega, V5280) and 1 µg of LysC (Wako Pure Chemical Corporation ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was achieved from 1 𝜇g of total RNA with M-MLV reverse transcriptase (RNase H minus, Point Mutant, Promega, USA) using an anchored oligo(dT)20 primer ...
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bioRxiv - Cancer Biology 2019Quote: ... which utilizes 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) (Promega, Madison WI). The assay was carried out according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: The 3’ UTRs were cloned into psiCheck-2 plasmid (Promega, USA, Cat. No. C8021) using the XhoI and NotI sites ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 µl of 5x SSIV buffer and 2 µl of DNase (RQ1, Promega) was added ...
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bioRxiv - Genetics 2020Quote: ... Cell growth after 4 days was measured using the CellTiterGlo 2 kit (Promega) and a GloMax luminometer (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: ... coelenterazine h (Promega) at 5 μM final concentration was added per well ...
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bioRxiv - Microbiology 2021Quote: ... Coelenterazine-H (Promega). Luminescence was read on a Glomax Multi+ Detection System (Promega) ...
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bioRxiv - Biophysics 2022Quote: ... coelenterazine h (Promega) at 5 μM final concentration was added per well ...
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bioRxiv - Cancer Biology 2022Quote: ... pMD2.G and the lentiviral gRNA plasmid at a 3:1:5 mass ratio using FuGENE HD (Promega) in Opti-MEM (Thermo Fisher Scientific) ...