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Citations for Promega :
401 - 450 of 4036 citations for 6 Pteridinepropanoic acid 2 4 diamino α 4 methoxycarbonyl phenyl α 2 propyn 1 yl methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 6 μl of Trypsin/LysC solution [100 ng/μl 1:1 Trypsin (Promega) and LysC (FujiFilm ...
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bioRxiv - Biochemistry 2022Quote: ... 6 μl of Trypsin/LysC solution (100 ng/μl 1:1 Trypsin (Promega) and LysC (FujiFilm) ...
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bioRxiv - Pathology 2022Quote: ... The infectious BSMV RNAs were transcribed in vitro from linearized plasmids γ-TaPDS, γ-TaCBS, γ-TaGSTU6, γ, α, and β (Petty et al., 1989) using the RiboMAX Large Scale RNA Production System-T7 kit (Promega, USA) and the Ribo m7G Cap Analog (Promega) ...
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bioRxiv - Cell Biology 2019Quote: ... the rein was equilibrated in IP buffer with 1 mM DTT and incubated overnight at 4°C with 5 μL ProTEV Plus (Promega, Sunnyvale, CA) in 100 μL total reaction volume ...
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bioRxiv - Cell Biology 2022Quote: ... anti-goat alkaline phosphatase-linked antibody and alkaline phosphatase substrate (5-bromo-4-choloro-3-indolyl 1-phosphate and nitroblue tetrazolium) from Promega (Southampton, UK); a hydroxamate-based MMP inhibitor CT-1746 (N1-[2-(S)-(3,3-dimethylbutanamidyl)]-N4-hydroxy-2-(R)-[3-(4-chlorophenyl)-propyl]-succinamide ...
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bioRxiv - Biochemistry 2019Quote: ... The washed beads were resuspended in 150 µl digestion buffer and incubated for 4 hours with 1 µg trypsin (Promega, catnr: V5111) at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The following day cells were trypsinized and seeded in 384-well white plate (20 µl/well) in DMEM F12 (no phenol red, 4% FBS) +/-HaloTag® NanoBRET™ 618 Ligand (1 µl/ml, Promega) and +/-compounds (DMSO concentration in each sample was kept the same) ...
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bioRxiv - Systems Biology 2023Quote: ... and digested using LysC 1:100 enzyme:proteins ratio for 4 hours (Wako sequencing grade, 125-05061) and trypsin 1:100 enzyme:proteins ratio for 16 hours (Promega sequencing grade, V5111). The digested proteins were then acidified with 10% (v/v ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested using LysC 1:100 enzyme:proteins ratio for 4 hours (Wako sequencing grade, 125-05061) and trypsin 1:100 enzyme:proteins ratio for 16 hours (Promega sequencing grade, V5111). The digested proteins were then acidified with 10% (v/v ...
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bioRxiv - Cell Biology 2021Quote: HEK293 T-REx SNAPf-GLP-1R-SmBiT cells were seeded in 12-well plates and co-transfected with 0.1 μg β-arrestin-2 fused at the N-terminus to LgBiT (LgBiT-β-arrestin-2; Promega, plasmid no. CS1603B118) and either 0.1 μg mini-Gs-Venus or mVenus supplemented with 0.8 μg pcDNA 3.1.
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bioRxiv - Molecular Biology 2023Quote: ... Figure 6 and Figure 7) to form condensates in cells was monitored by transient transfection of U-2 OS cells using FuGene (Promega; Figures 2 and 7) or electroporation (as before ...
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bioRxiv - Cell Biology 2020Quote: ... following manufacturer’s instructions using 200 ng psiCHECK™ 2 (Promega) as empty vector control or psiCHECK2 containing Tnf-α 3’ UTR or Il6 3’ UTR and 30 pmol miRNA scramble negative control ...
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bioRxiv - Molecular Biology 2019Quote: A commercial kit (HDAC-Glo™ 2 Assays, Promega, G9590) was used to measure HDAC2 activity ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... samples were treated with 2 U/mL DNase I (Promega) for 1 min at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... After adding 2 μl of trypsin (0.5 μg/μl, Promega), peptide mixtures were incubated at 37°C for 4 hrs ...
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bioRxiv - Biochemistry 2020Quote: ... Before adding 2 μl of trypsin (0.05 μg/μl, Promega), 500 fmol of isotope-labeled peptides 1 and 2 were added to spike the ROS preparation ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µg of total RNA was treated with DNAse (Promega) and utilized as a template for the cDNA synthesis reaction using MMLV reverse transcriptase (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... Amplified DNAs were cloned into pSiCHECK-2 (Promega, Wuhan, China) by utilizing XhoI and NotI restriction enzyme sites to construct pSi2-hUTR and pSi2-mUTR ...
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bioRxiv - Neuroscience 2020Quote: ... using the GoTaq® 2-step RT-qPCR kit (Promega). Quantitative PCR runs were performed on a 7900HT Fast Real-Time PCR System (Applied Biosystems Inc. ...
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bioRxiv - Biochemistry 2020Quote: ... T7 RNA polymerase and 2 µL RNasin RNase inhibitor (Promega) and incubated overnight at 37°C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Polymerase chain reactions were performed using 2 U Gotaq (Promega), 1X Gotaq buffer (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... 2 μg of 0.5 μg/μl sequencing grade trypsin (Promega) was added ...
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bioRxiv - Microbiology 2021Quote: To clone the 3xant-miR-275 into psiCheck-2 (Promega), two complementary single-stranded oligos that encode 3xant-miR-275 and XhoI and NotI restriction endonuclease cut sites were synthesized at Yale Keck Oligo Synthesis Resource (Table 1) ...
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bioRxiv - Cell Biology 2022Quote: ... the protein suspension was digested with 2 µg trypsin (Promega) overnight at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were transfected with psiCHECK-2 plasmids (Promega, Madison, USA) containing two luciferase genes ...
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bioRxiv - Cancer Biology 2023Quote: ... MTS reagent (2 mg/ml stock; Cat. No. G1111, Promega) was freshly supplemented with phenazine methosulfate (PMS ...
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bioRxiv - Microbiology 2023Quote: ... psiCHECK2-M was a modified form of psiCHECK-2 (Promega) with deletion of the HSV-TK promoter (Figureure 5A) ...
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bioRxiv - Microbiology 2023Quote: ... and 2× GoTaq qPCR Master Mix (Promega, Madison, WI, USA). RT-qPCR was performed using the MyGo Pro real-time PCR instrument (IT-IS Life Science Ltd. ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µL of 50% Cell-Titer Glo (Promega cat G7573) diluted in water was added to the cells and luminescence measured on an EnVision Plate Reader (PerkinElmer).
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bioRxiv - Molecular Biology 2022Quote: ... and 2% of beetle luciferin sodium salt (Promega, Madison, USA). For luminescence measurements ...
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bioRxiv - Cancer Biology 2023Quote: ... cell viability was measured using CellTiter-Glo 2 reagent (Promega). After addition of the reagent to cell culture medium ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was detected using CellTiter Glo 2 reagent (Promega). After addition of the reagent to cell culture medium ...
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bioRxiv - Neuroscience 2023Quote: ... 2) an unrelated male fibroblast genomic DNA sample (Promega, G1471), and 3 ...
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bioRxiv - Molecular Biology 2023Quote: ... and GTaq® qPCR Master Mix (Promega, Cat.# A6001/2) were utilized.
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bioRxiv - Molecular Biology 2023Quote: ... viability was measured using CellTiter-Blue assay (G8081/2, Promega) following the protocol of the manufacturer ...
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bioRxiv - Plant Biology 2023Quote: ... 2 μg of total RNA treated with DNase RQ1 (PROMEGA) was reverse transcribed with Superscript IV (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 2-3 µg bacmid DNA was transfected (FuGene HD, Promega) into 2 ml Sf9 cells plated at 0.5x106 cells/ml density in a 6-well plate and left to incubate for 5-7 days 27ºC without shaking ...
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bioRxiv - Systems Biology 2024Quote: ... before adding 2 µL of 0.5 ng/µL trypsin (Promega) in digestion buffer (50 mM TEAB ...
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bioRxiv - Cell Biology 2024Quote: ... the protein suspension was digested with 2 µg trypsin (Promega) overnight at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mg/mL 50 μL aliquots of cleared lysates were incubated with 2 μL of indicated trypsin concentration (Promega) at 4°C for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were lysed after 72 h (2+1 DIV for differentiations) using the Dual Luciferase Reporter kit (Promega, E1960) and luciferase activity was measured using a Victor3 (Perkin Elmer ...
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bioRxiv - Microbiology 2021Quote: ... A partial trypsin digestion was performed to release proteins from the beads by using 80 μL of 2 M urea/50 mM Tris containing 1 mM DTT and 0.4 μg trypsin (Mass Spectrometry Grade, Promega) for 1 h at 25 C ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 mini tablet of Complete EDTA-free protease inhibitor and 2 µl RNAse inhibitor (RNAsin Plus RNase Inhibitor, Promega). RNA was extracted using the RNASpin Mini kit (GE Healthcare ...
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Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: Supernatants were incubated 1:2 with the substrate solution of the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega) for 30 min in the dark in a 96-well plate (Greiner) ...
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bioRxiv - Biochemistry 2020Quote: ... before being washed and resuspended in 2 M urea and trypsinized overnight with 0.5 μg μl−1 sequencing grade trypsin (Promega). Tryptic peptides were eluted off ...
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bioRxiv - Molecular Biology 2022Quote: ... Semiquantitative PCR reactions were carried out with 1–2 μL of cDNA and 0.2 μL of GoTaq DNA Polymerase (Promega) in a total reaction volume of 30 μL according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... to a final urea concentration of 2 M for overnight Trypsin/Lys-C digestion at 35°C (1:100 protease:substrate ratio, Mass Spectrometry grade, Promega Corporation ...
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bioRxiv - Neuroscience 2022Quote: ... HEK cells were transfected with the targeting plasmid and two helper plasmids (psPAX2 and pMD2.G) at a 2:1.5:1 molar ratio using ProFection (Promega). Two to three days later ...
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bioRxiv - Cell Biology 2022Quote: ... The gel pieces were rehydrated in trypsin solution (50 mM ABC pH 8.0, 1 or 2 µg trypsin per sample for proteome or BioID respectively, Promega) overnight at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Then the beads were incubated with 80 μL of 2 M urea in 50 mM Tris-HCL containing 1 mM DTT and 0.4 μg trypsin (Promega) at 25 °C for 1 hour while shaking at 1,000 rpm ...