Labshake search
Citations for Promega :
4351 - 4400 of 4516 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2022Quote: ... Protein concentration in the supernatant was measured using a NanoDrop and 1:200 (w/w) of trypsin (Promega, Mass Spectrometry grade) was added to 200 µg of sample ...
-
bioRxiv - Microbiology 2022Quote: ... RNA samples and the negative extraction controls were screened in duplicate to investigate the presence of YFV RNA by RT-qPCR (GoTaq Probe 1-Step RT-qPCR System-Promega, USA), with primers and probes targeting the 5’-noncoding regions of the YFV genome (39) ...
-
bioRxiv - Cell Biology 2024Quote: ... After PCR pre-amplified cDNA were clean-up using 22% home-made PEG beads at a ratio of 0.7 beads:1 sample before concentration quantification using QuantiFluor® dsDNA System (Promega, #E2671). cDNA was normalised and diluted to 100pg µl-1.
-
bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was achieved from 1 𝜇g of total RNA with M-MLV reverse transcriptase (RNase H minus, Point Mutant, Promega, USA) using an anchored oligo(dT)20 primer ...
-
bioRxiv - Bioengineering 2024Quote: ... were taken for overnight digestion with mass spec grade Trypsin/Lys-C Mix (Promega, Madison, WI, cat. No. V5071, 1:20). Samples were reduced with TCEP (final concentration 5 mM ...
-
bioRxiv - Biochemistry 2024Quote: ... when the temperature was dropped to 22°C and isopropyl β-d-1-thiogalactopyranoside (IPTG) and L-rhamnose (Promega, Madison, WI) was added at 0.2 mM and 0.1% (w/v ...
-
bioRxiv - Biochemistry 2024Quote: HEK293T cells were transfected in a 6-well plate with 1 μg ADGRG6 DNA and 3 μL transfection reagent Fugene 6 (Promega, PRE2693) at 60-70% confluency and incubated for 48 h ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were transfected the following day using 0.6–1 µg of plasmid and Viafect reagent (Promega E4981, 6 µl/µg plasmid). The medium was exchanged the following day to regular culture medium ...
-
bioRxiv - Cell Biology 2024Quote: ... hiPSC-aCM or HEK293 cells were incubated with a labeling solution containing both cell-impermeant HTL-Alexa488 (Promega G1001, 1 µM) and Cellmask Deep Red Plasma Membrane stain (Invitrogen C10046 ...
-
bioRxiv - Developmental Biology 2024Quote: ... rat anti-Fat (kind gift from Helen McNeill) was used at 1:500 and mouse anti-β-galactosidase (Z3781, Promega; RRID:AB_430877) was used at 1:500 ...
-
bioRxiv - Cell Biology 2024Quote: ... images produced from HeLa cells seeded on glass-bottom Mattek dishes (P35GC-1.5-14-C) and transfected with TFG-mEGFP-FLAG at 1 µg/µL using Fugene (Promega, cat # PRE2311) for 24 hours ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were washed thoroughly in PBS-T before incubation with secondary antibodies (anti-rabbit horseradish peroxidase, Promega WB401B 1:2,500 dilution) for 1 h at room temperature ...
-
bioRxiv - Molecular Biology 2022Quote: ... 20 ng of cDNA of each sample was used for the qPCR reaction (duplicate/sample) with ENG or α-tubulin specific primers (Supp. Table 1) and the GoTaq qPCR Master mix (Promega), in presence of CXR reference dye ...
-
bioRxiv - Plant Biology 2022Quote: ... Total RNA (1 μg) was reverse-transcribed using oligo(dT)18 and M-MLV reverse transcriptase II (Promega, Fitchburg, WI, USA).
-
bioRxiv - Microbiology 2023Quote: ... Nanoluciferase bioluminescence was measured from the Hyp1-Nluc parasites by lysing the parasite culture 1:5 with 1x Nano-Glo Lysis Buffer (Promega, USA), and the addition of 1:1000 Nano-Glo (Promega) ...
-
bioRxiv - Cell Biology 2023Quote: ... samples were resuspended in 50 µl of 50 mM ABC buffer and digested at 37°C for 16 h using 1 µg of trypsin (Trypsin Gold, Promega, V5280) and 1 µg of LysC (Wako Pure Chemical Corporation ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were alkylated using 15 mM chloroacetamide at 37° C for 30min and further digested using sequencing-grade modified trypsin (1/50 w/w, ratio trypsin/protein; Promega, USA) at 37°C for 12h ...
-
bioRxiv - Biochemistry 2023Quote: ... nLuc luciferase signal was measured by mixing 25 μL of Nano-Glo Luciferase Assay System (prepared 1:50 as recommended; Promega # N1120) with 25 μL of diluted reactions (diluted 1:5 in Glo Lysis Buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... 30 min) and diluted to 1M Urea with 200 mM ABC for trypsin digestion (1 μg, 37°C, overnight shaking, Promega, #V5113). On the next day ...
-
bioRxiv - Molecular Biology 2023Quote: ... transfected cells grown for 48 hours in 96-well plates were washed with Hanks′ Balanced Salt solution (HBSS) and incubated with 1/1,000 dilution of furimazine stock solution (Promega, WI, USA). After incubation for 2 minutes ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Membranes were washed in TBST and probed with anti-mouse HRP secondary antibody (Promega; 1:2000 in 5% BSA in TBST) for 1 h at room temperature followed by development in ECL (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2023Quote: ... from the access handles by running this on a 1% agarose gel and gel purify the right DNA fragment with a gel purification kit (Promega, A9282).
-
bioRxiv - Biochemistry 2023Quote: ... The reaction was quenched with DTT and urea was diluted to <1 M with 50 mM NH4HCO3 before adding MS-grade trypsin (Promega, V5113) in a ratio of 1:25 (trypsin:protein ...
-
bioRxiv - Cancer Biology 2022Quote: ... to 10.5mL of 2.0 x 10^5 of Hek293 Cells (ATCC CRL1573) in growth media (DMEM + 10% FBS) was added 525uL of a 9:1 ratio of carrier DNA (Promega E4881) to a CLK nLuc construct-CLK1 CLK2 or CLK4 (Promega – NV1131 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Reactions were incubated for 1 hr at 30 °C and luciferase signal was measured using Dual-Glo Luciferase Assay System (Promega E2920).
-
bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed 3 × 10 min in TBS-T (0.1%) before 1hr incubation in secondary antibodies (anti-rabbit HRP or AP conjugate (Promega; 1: 5.000)) ...
-
bioRxiv - Cell Biology 2023Quote: HeLa FlpIn were co-transfected with pCDNA5 constructs with gene of interest and construct of pOG44 recombinase in a 1:9 ratio with Fugene HD (Promega, E2311) under the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... The sample was then diluted 50% with PBS before addition of sequencing grade trypsin (1 μg per sample, Promega Corp, V5111) and incubated overnight at 37 °C ...
-
bioRxiv - Microbiology 2023Quote: ... The culture was diluted 1:100 in fresh LB supplemented with chloramphenicol and kanamycin including a 1:5000 dilution of the Nano-Glo® Luciferase Assay Substrate (Promega). Preliminary growth curves in the presence of the substrate showed no impact on growth at this concentration ...
-
bioRxiv - Cell Biology 2024Quote: ... 5 µL of the schizont culture was aliquoted into white 96 well luminometer plates to which 45 µL of 1x NanoGlo Lysis buffer with 1:1000 NanoGlo substrate (Promega, USA) was added ...
-
bioRxiv - Cancer Biology 2024Quote: ... The reaction mixture was then transformed at a ratio of 1:10 (reaction volume/volume competent cells) into HB101 competent cells (Promega #L2011). Spectinomycin-resistant colonies were screened by restriction digestion of miniprep DNA and subsequently validated by gel electrophoresis in a 0.8% agarose gel and by DNA sequencing ...
-
bioRxiv - Systems Biology 2024Quote: ... protein digestion was performed overnight by addition of 50 nL of 0.01 ng nL-1 Lys-C (MS grade, Promega, Madison, USA) and 50 nL of 0.04 ng nL-1 trypsin (Promega ...
-
bioRxiv - Genomics 2023Quote: ... cells were washed once with cold 1× PBS and the luciferase activities were measured with Perkin Elmer Envision using Promega Dual Luciferase Assay kit (Promega, E1960). The firefly luciferase activity was normalized to renilla luciferase activity for each well ...
-
bioRxiv - Biochemistry 2023Quote: ... samples were resuspended in 6 M guanidine hydrochloride.250 µg of protein were digested overnight at 37 °C using trypsin with a 1:20 trypsin to sample ratio (Promega, #V5117). The next day ...
-
bioRxiv - Biochemistry 2023Quote: ... The sample was then diluted 50% with PBS before addition of sequencing grade trypsin (1 μg per sample, Promega Corp, V5111) and incubated overnight at 37 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... Samples were lyophilized and dissolved in digestion buffer (50 mM ammonium bicarbonate [ABC], 1 mM calcium chloride) and digested overnight using trypsin (Promega, #V5117) at trypsin ...
-
bioRxiv - Microbiology 2023Quote: ... Standard curves were generated using purified genomic stocks (HSV-1 BAC, KSHV BAC, human genome Promega #G1471, mouse genome Promega #G3091), or purified plasmids (MHV68 ORF50) ...
-
bioRxiv - Microbiology 2023Quote: ... Standard curves were generated using purified genomic stocks (HSV-1 BAC, KSHV BAC, human genome Promega #G1471, mouse genome Promega #G3091), or purified plasmids (MHV68 ORF50) ...
-
bioRxiv - Bioengineering 2023Quote: ... and loaded on a micro S-trap column (Protifi, NY) and digested according to vendor protocol for 1 hour at 50 C by Trypsin Platinum (Promega, WV). Digested material was then eluted from the column by centrifuge at 1000 rpm for 2 minutes and dried in speedvac (Eppendorf ...
-
bioRxiv - Systems Biology 2024Quote: ... and an aliquot of approximately 25 µg of protein was digested with Trypsin (Promega, Trypsin to protein ratio of 1:50) at 37°C overnight ...
-
bioRxiv - Physiology 2024Quote: ... Protein (50 μg per sample) was digested overnight at 37°C with LysC (Wako Chemicals) at 1:50 (w/w) ratio and trypsin (Promega, V5113) at 1:100 (w/w ...
-
bioRxiv - Biochemistry 2024Quote: ... 20 µg of protein was diluted 20 times with 50 mM ABC (no guanidine) and trypsin was added with a 1:20 trypsin to sample ratio (Promega, V5117). Protein were digested overnight at 37 °C ...
-
bioRxiv - Cell Biology 2024Quote: ... Membranes were washed 3 times in TBST for 15’ per wash and incubated 1 hour in HRP-conjugated anti-rabbit (Promega, W4011) or anti-mouse (Promega ...
-
bioRxiv - Neuroscience 2024Quote: ... The homogenate was centrifuged at 16,000 x g for 10 min and the supernatant was collected and diluted to 1:200 with deiodinized water for ATP measurement using the Cell Titer-Glo luminescent cell viability assay kit (Promega, G7571) and 1:10 for determining the protein concentration ...
-
bioRxiv - Biochemistry 2024Quote: ... 1 µg of protein was diluted 5 times with 50 mM ABC and trypsin was added with a 1:20 trypsin to sample ratio (Promega, V5117). Protein were digested 24 h at 37 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... 20 µg of protein was diluted 10 times with 50 mM ABC (no Guanidine) and trypsin was added with a 1:20 trypsin to sample ratio (Promega, V5117). Protein were digested overnight at 37 °C ...
-
bioRxiv - Immunology 2024Quote: HEK293T cells (∼6 × 105 cells per 6-well) were transfected with 1-3 μg of the indicated constructs using FuGENE® HD Transfection Reagent (#E2311, Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... 20 µg of protein was diluted 20 times with 50 mM ABC (no guanidine) and trypsin was added with a 1:20 trypsin to sample ratio (Promega, V5117). Protein were digested overnight at 37 °C ...
-
bioRxiv - Biochemistry 2020Quote: ... The sample was then incubated at 37°C for overnight trypsin digestion (trypsin 1:50 protein w/w ratio; Promega, Madison, WI). The digestion reaction was quenched by adding formic acid (FA ...
-
bioRxiv - Cancer Biology 2021Quote: ... DNA:FuGENE complexes were formed at a ratio of 1:3 (μg DNA/μL FuGENE HD) according to the manufacturer’s protocol (Promega, Madison, WI, USA). The resulting transfection complex (1 part ...