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Citations for Promega :
4201 - 4250 of 5281 citations for 6 methyl 4 oxo N phenyl 2 3 dihydro 1 4 oxathiine 5 carboxamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... USA) and 10 μl of GoTaq 1-Step RT-qPCR (Promega, Madison, WI, USA). Thermal cycling was performed at 50°C for 15min for reverse transcription ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using the GoTaq® 1-Step RT-qPCR System (Promega). Knockdowns were verified using the primers ...
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bioRxiv - Biochemistry 2020Quote: ... Proteomic digestion was performed by the addition of 1 μg proteomics-grade Trypsin (Promega) and incubated overnight at 37 °C ...
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bioRxiv - Biophysics 2021Quote: ... 20μL of a 1/5th diluted solution of Bright-Glo reagent (Promega, Madison, WI) was added directly to the wells ...
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bioRxiv - Neuroscience 2021Quote: ... USA) at 1:10 dilution and polyclonal HaloTag antibody (Promega Corp. Madison, WI, USA) at 1:500 dilution and FITC-conjugated donkey anti-mouse secondary antibody and Cy3-conjugated goat anti-rabbit secondary antibody (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... 9 μl purified RNA was incubated with 1 μl Oligo(dT)15 primers (Promega) at 80°C for 3 min ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by an overnight trypsin digestion (ratio 1:33 enzyme:lysate, Promega, Madison, WI, USA).
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bioRxiv - Cancer Biology 2020Quote: ... Plates were incubated for 1 h at RT and luminescence recorded (Glowmax Luminometer, Promega) and then compared to Glucose/Lactate standards to determine relevant concentrations.
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bioRxiv - Plant Biology 2021Quote: ... ground into powder and dissolved in 200 μL 1 × Cell Culture Lysis Reagent (Promega), followed by centrifugation for 15 min at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... 40 ng ml-1 of Renilla luciferase under a constitutive promoter (pRL-TK, Promega), and 40 ng ml-1 of pLEXm vector (34 ...
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bioRxiv - Genetics 2021Quote: ... 1 μg linearized vector was purified with the PureYield Plasmid Miniprep System (Promega, A1223) and used as template for in vitro synthesis of capped mRNA with the Ambion mMessage Machine T3 Transcription Kit (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... Afterwards 40 µL digestion buffer (1:10 diluted Rapid Digest buffer (Promega, Fitchburg, WI) in water ...
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bioRxiv - Microbiology 2021Quote: ... incubated for 1 h at RT and analysed using the GloMax multidetection system (Promega). Parasitaemia was used to normalise parasite density between samples and replicates ...
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bioRxiv - Microbiology 2021Quote: ... cDNAs were synthesized from 1 μg of total RNA using AMV reverse transcriptase (Promega) and used directly for quantitative PCR (qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 μg of mass spectrometry grade trypsin/Lys-C mix (Promega CAT# V5071) in 60 μl of 50 mM ammonium bicarbonate was added to each sample ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were digested at 37 °C overnight with trypsin (Promega; 1:10, enzyme/substrate) in the presence of 10% acetonitrile ...
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bioRxiv - Systems Biology 2022Quote: ... and RNA (1.5μg) was treated with RQ1 Dnase I (Promega; 1 U/µg RNA). cDNA was synthesized with a qPCRBIO high-quality cDNA synthesis kit ...
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bioRxiv - Microbiology 2022Quote: ... cDNAs were synthesized from 1 μg of total RNA using AMV reverse transcriptase (Promega) and used directly for quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2022Quote: ... Digestion was performed with trypsin in a ratio of 1:20 m:m protein:trypsin (Promega, P/N V5072 for the pilot study ...
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bioRxiv - Microbiology 2020Quote: ... cDNAs were synthesized from 1 μg of total RNA using AMV reverse transcriptase (Promega), and used directly for quantitative PCR (qPCR ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfected cells were lysed in 12.6 μl of 1 × Passive Lysis Buffer (PLB, Promega) and light emission was measured following injection of 25 μl of either Renilla or Firefly luciferase substrate ...
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bioRxiv - Neuroscience 2021Quote: ... Cells used for growth cone analyses were stained for βIII-tubulin (1:1000, Promega), Phalloidin 568 conjugate (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg cellular RNA was treated with DNase (RQ1 Rnase-Free DNase kit Promega) and reverse-transcribed following the GoScript ™ Reverse Transcriptase Kit (Promega ...
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bioRxiv - Microbiology 2020Quote: ... which were destained and overnight-digested with trypsin (1 μg/mL, sequencing grade, Promega), before MS analysis of the peptide mixes ...
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Toxoplasma GRA15 limits parasite growth in IFNγ-activated fibroblasts through TRAF ubiquitin ligasesbioRxiv - Immunology 2020Quote: ... culture supernatants were removed and 1× lysis buffer was added (Luciferase assay system, Promega) to the cells in the wells ...
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bioRxiv - Bioengineering 2021Quote: ... The bottom of the chamber was coated with 10 μg ml−1 streptavidin (Promega) overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... after which 25 µL assay buffer supplemented with 1:25 furimazine substrate (Promega, N246A) and 1:250 goat anti-rabbit Alexa Fluor 594 was added ...
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bioRxiv - Microbiology 2022Quote: ... 1 µL of this reaction was diluted in 100uL of passive lysis buffer (Promega). Following this ...
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bioRxiv - Molecular Biology 2022Quote: LNCaP/Luc or transfected COS-1 cells were lysed with Reporter Lysis Buffer (Promega). Luciferase activity was assayed using the Steadylite Plus kit (PerkinElmer) ...
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bioRxiv - Cell Biology 2022Quote: ... RNA (1 μg) was reverse transcribed with ImProm-II Reverse Transcriptase (Promega, Madison, Wisconsin) using oligo(dT ...
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bioRxiv - Biochemistry 2019Quote: ... Enzymatic digestion was performed by addition of 1 μg trypsin (Gold, Promega, Madison USA) in TEAB 100 mM and incubation overnight at 30°C ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... we used a 1/1500 artificial mix of promastigote DNA and human DNA (Promega) to reflect the median ratio found in clinical samples ...
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bioRxiv - Pathology 2021Quote: ... and anti-rabbit IgG antibody coupled to horseradish peroxidase (Promega France, W401B, 1/2500) and revealed with the Western Lightning chemiluminescence reagent Plus (PerkinElmer Life Sciences) ...
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bioRxiv - Microbiology 2020Quote: ... for 1 h at 55°C then with 200 μg/mL proteinase K (Promega) for 1 h at 55°C ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were then lysed using 1× Passive Lysis Buffer as per manufacturer’s protocol (Promega). Protein concentration was then determined via Bradford assay ...
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bioRxiv - Molecular Biology 2019Quote: ... The final reaction volume contained 1-fold concentrated T4 ligase buffer (Promega, Madison, US). Prepared reaction mixtures (ligase buffer ...
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bioRxiv - Genomics 2021Quote: ... Samples were then treated for 1 hour at 37°C with RQ1 DNase (Promega) to remove possible contamination by DNA ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were digested overnight at 37 °C using trypsin (1:50, w/w, Promega). Peptides were extracted in ACN and formic acid and combined extracts were freeze-dried ...
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bioRxiv - Genomics 2020Quote: ... 0.2 μL GoScript™ RT Mix for 1-Step RT-qPCR (Promega, Madison, WI), 0.75 μL primer/probe sets for either N1 ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.5) containing 1% Tween20 and 100 U/ml RNasin Plus RNase Inhibitor (Promega). After centrifugation at 15,000 g for 10 min at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... and Dad13 constructs were detected with using mouse-anti-β-galactosidase (1:1000, Promega) and rabbit-anti-β-galactosidase (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmid constructs (pCAG nHA GPC4, see Table 1) were transfected with Fugene (Promega E2691)/ opti-MEM mixture according to manufacturer protocols ...
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bioRxiv - Genomics 2021Quote: ... cDNA was synthesized from 1 μg RNA using M-MLV reverse transcriptase (9PIM170, Promega) and Oligo(dT)15 primers (C110A ...
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bioRxiv - Cancer Biology 2021Quote: ... or at 25°C with Glu-C (Promega; 1:20 protease:protein w/w ratio) overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... 50 μl of 0.5M TEAB and trypsin (Promega, enzyme: protein ratio of 1:50) were added to the membrane ...
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bioRxiv - Microbiology 2021Quote: ... and digested with sequencing grade trypsin (Promega, 1:50 trypsin- to-protein [wt/wt]) (Yang et al. ...
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bioRxiv - Plant Biology 2021Quote: ... The following components were added: (1) M-MLV reverse transcriptase (Promega, Cat. No. M1701), 1 mM dNTP mix ...
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bioRxiv - Bioengineering 2022Quote: ... cells in each well were lysed in 30 μl 1× passive lysis buffer (Promega). 10 μl per well of lysate was assayed using the Dual Luciferase Assay kit (Promega).
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bioRxiv - Genetics 2022Quote: Type-1 quantitative bioluminescence resonance energy transfer (qBRET) experiments utilised the NanoBRET platform (Promega) and followed the general type-1 qBRET protocol reported by Felce et al.25 The assay was performed in HEK293 cells transiently expressing POPDC1 and POPDC2 constructs which possessed a C-terminal NanoLuc luciferase or HaloTag domains ...
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bioRxiv - Plant Biology 2022Quote: ... The digestion solution was diluted to <1 M urea upon addition of trypsin (Promega, V511A ...