Labshake search
Citations for Promega :
4001 - 4050 of 4642 citations for Mouse Vasoactive intestinal polypeptide receptor 2 VIPR2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... The reporter plasmids and β-gal expression plasmids were cotransfected in K562 cells and luciferase assay was carried out after 48 hours using Nanoglo luciferase kit (Promega) and normalized to ß-gal expression measured using assay reagent (ThermoFisher).
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bioRxiv - Plant Biology 2020Quote: ... The overlapping PCR products were separated and purified by the Spin Column PCR Product Purification Kit (Wizard SV Gel and PCR Clean-Up System) following manufacturer’s instruction (Promega, WI). Then ...
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bioRxiv - Bioengineering 2020Quote: We used the method of ATP-based luminescent cell viability assay with the Kit named CellTiter-Glo Luminescent Cell Viability Assays (Promega). 5×104 cells in 100 μL of DMEM medium per well were seeded in a 96-well opaque white polystyrene microplate (Corning ...
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bioRxiv - Molecular Biology 2020Quote: ... RNase digestion of cellular RNA was verified by purifying RNA from supernatant after immunoprecipitation using Reliaprep RNA purification kit (Promega) and then analyzing it by denaturing formaldehyde-agarose gel electrophoresis followed by imaging of the ethidium bromide stained gel.
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bioRxiv - Molecular Biology 2020Quote: The AP-1 luciferase reporter was expressed using an adenoviral vector and luciferase activity determined using a luciferase assay kit from Promega as previously described (Higazi et al. ...
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bioRxiv - Systems Biology 2020Quote: ... were detected by the alteration of the dose-response curve measured using CellTiterGlo Chemoluminescent Kit (Promega Corporation, Cat. No.: G7573). Cell lines were confirmed to be mycoplasma-negative using the Mycoalert PLUS Mycoplasma detection kit (Lonza ...
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bioRxiv - Microbiology 2020Quote: ... coli was grown in NB liquid medium (180 rpm, at 30 ° C) and had its DNA extracted with Wizard Genomic DNA Purification Kit (Promega). The number of bacteria in the seed and root was calculated based on the values of Ct (cycle threshold ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were harvested at various time points of differentiation over a period of 11 days and then analyzed using a Dual-Glo luciferase assay kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... and then excised and purified using the Wizard® SV Gel and PCR Clean-Up System kit (Promega, Madison, WI) according to manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... Amplification products were checked on a 1% agarose gel and were purified using the Wizard PCR clean-up kit (Promega). DNA concentration and purity were measured using a ND-1000 UV-Vis Spectrophotometer (Nanodrop Technologies).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were washed once with PBS and their viability was measured with a kit designed to quantify ATP level according to instructions of the manufacturer (CellTiterGlo Luminescent Cell Viability Assay, Promega).
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bioRxiv - Microbiology 2020Quote: RNA was extracted from 300□μl of plasma using the Viral Total Nucleic Acid Purification kit (Promega, Madison, WI, USA) on a Maxwell 16 MDx instrument ...
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bioRxiv - Microbiology 2020Quote: ... coli was grown in NB liquid medium (180 rpm, at 30 °C) and had its DNA extracted with Wizard Genomic DNA Purification Kit (Promega). The reference DNA was diluted in series of 10−2 to 10−8 (20 – 2 × 109 ng of DNA) ...
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bioRxiv - Microbiology 2020Quote: ... Cell viability was evaluated using the MTS-based CellTiter 96® AQueous One Solution Cell Proliferation Assay kit (Promega, G3582), which determines viable cell number measuring the conversion at 490 nm of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS ...
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bioRxiv - Microbiology 2020Quote: ... The amounts of LDH were measured spectrophotometrically using a Cyto-Tox 96 Non-radioactive Cytotoxicity Assay Kit (Promega, Madison, WI). We added 50 μL of 10 %Triton X-100 to the 950 μL of extracellular medium of L2 cells in 24-well plate ...
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bioRxiv - Microbiology 2020Quote: ... The full-length cDNA and EcoRI digested pKTO-IBV-N were subjected to in vitro transcription using T7 transcription kit (Promega), respectively ...
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bioRxiv - Microbiology 2021Quote: ... from 12-72 hpt and luciferase activity was measured using the Dual Luciferase reagent kit and GloMax multi detection system (Promega). Firefly luciferase readings from the TBEV replicons were normalized to the Renilla values (pTK-Ren ...
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bioRxiv - Microbiology 2021Quote: Extraction of cellular DNA and viral DNA was conducted using Wizard® Genomic DNA Purification Kit (Promega, Madison, Wisconsin, USA) and QIAamp MinElute viral DNA extraction kit (QIAGEN ...
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bioRxiv - Plant Biology 2020Quote: ... Gene PCR products of 200-300 bp were labelled with 32P-dCTP following the prime-a-gene labelling kit instructions (Promega) and used as probes (Supplementary Table 1) ...
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bioRxiv - Genetics 2021Quote: ... Cell death was analyzed similarly using a commercially available kit that labels fragmented DNA from apoptotic cells (DeadEnd Fluorometric TUNEL System, Promega).
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bioRxiv - Pathology 2021Quote: ... Cell viability was monitored by measuring lactate dehydrogenase (LDH) leakage into the culture medium by using the CytoTox96 Non-Radio Cytotoxicity Assay kit (Promega). For adherent cells (HFFs ...
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bioRxiv - Synthetic Biology 2020Quote: Cells from HAC-containing clones have been harvested and genomic DNA has been collected using Maxwell DNA purification kit (Promega). qPCR analysis have been performed using SYBR Green Master Mix (Roche ...
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bioRxiv - Zoology 2020Quote: ... Genomic DNA was extracted from the selected body part using Promega Wizard® Genomic DNA Purification kit (A1120, Promega, UK) and DNA dissolved with 100 ul of molecular water and stored in −20°C for the future use.
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bioRxiv - Microbiology 2020Quote: ... PCR reaction mixtures were solved in 1% (w/v) agarose gel and purified by means of Wizard SV gel and PCR Clean Up kits (Promega). The amplified gene was treated with Taq polymerase (Fermentas ...
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bioRxiv - Cancer Biology 2021Quote: Proliferation was measured by a MTS conversion assay using the CellTiter 96 Aqueous Non-Radioactive Cell Proliferation kit (#G5421, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was purified using the Direct-Zol RNA Microprep Kit (Ozyme) according to the recommendations provided and was quantified using the QuantiFluor RNA system (Promega). One μl of 10 μM of oligo-dT primer and 1 μl of 10 μM of dNTPs were added to 0.3 ng of total RNA in a final volume of 2.3 μl ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were cultured in the presence of signaling proteins for another 24 hours before being washed and lysed as suggested by the instructions in the Dual Luciferase Assay Kit (Promega) manual ...
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bioRxiv - Biophysics 2019Quote: ... coli HB101 cells and isolated in quantity by using a Promega Wizard Plus Megaprep purification kit (Promega Corp., Madison, WI).
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bioRxiv - Microbiology 2019Quote: High-molecular weight genomic DNA of bacteria was isolated from 5 mL overnight cultures using the Wizard genomic DNA isolation kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: Southern blotting was performed in accordance Koch et al.19 Genomic DNA was prepared using the Wizard Genomic DNA Purification kit (Promega) and digested with SspI-HF and MfeI-HF (NEB) ...
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bioRxiv - Genomics 2019Quote: ... we extracted the genomic DNA from two samples of each group using the Wizard Genomic DNA purification kit (Promega, A1120). The DNA concentration was measured by the Qubit dsDNA Broad-Range Assay (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... Cells from each cell line were cultured for an additional 48 hours until total RNA isolation (Maxwell RSC simplyRNA kit, Promega).
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bioRxiv - Genetics 2020Quote: The frameshifting efficiency of the reporter plasmids in cultured cells were assayed as described previously (15, 16) using a dual luciferase reporter assay system kit (Promega). 24 hours post transfection ...
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bioRxiv - Immunology 2021Quote: Toxicity was measured by quantifying the cell viability using the CytoTox-ONE™ Homogeneous Membrane Integrity Assay kit (Promega, G7891) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... cell viability followed by caspase 3/7 activity were measured using CellTiter-Fluor™ Cell Viability Assay kit (Promega, G6080) and Caspase-Glo® 3/7 Assay System (Promega ...
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bioRxiv - Evolutionary Biology 2021Quote: ... An overhang was added to purified Or67b amplicons with Taq polymerase (Fermentas) and cloned using the pGEM-T Easy cloning kit protocol (Promega). Plasmids were extracted and purified using the GenElute plasmid miniprep kit (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: Cell viability assays were performed by measuring the cellular ATP level using a Cell Titer-Glo Luminescent Cell Viability Assay kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... RNAs were reverse transcribed to produce cDNAs by using the GoScript™ Reverse Transcription System Kit (Promega, cat. no. A5003). The cDNAs were then used for qPCR to evaluate gene expression ...
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bioRxiv - Microbiology 2021Quote: ... as described previously(40) and 1 μg of RNA was reverse transcribed to cDNA using the ImPromIITM Reverse Transcriptase kit (Promega), according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: ... Firefly (Fluc)- and Renilla (Rluc)-luciferase activity in each cell lysate was determined using the Dual Luciferase Assay Kit (Promega) and a Berthold tube luminometer ...
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bioRxiv - Cell Biology 2021Quote: GAP-accelerated GTP hydrolysis of Rab was measured by using a GTPase-Glo™ assay kit (Promega, Madison, WI; #V7681) according to the manufacture’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed 24 hours post-transfection with 1x Passive Lysis and reporter activities measured using a dual luciferase kit (Promega).
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bioRxiv - Microbiology 2021Quote: ... n=4 vaginal) and rUTI (n=3 urine) cohorts belonging to B2 phylogenetic group was extracted (Wizard Genomic DNA Purification Kit, PROMEGA) and sequenced by Illumina NovaSeq 2×150 nt ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was extracted from a small piece of muscle tissue by using a Maxwell RSC Blood DNA Kit with a Maxwell RSC Instrument (Promega). A shotgun DNA library was constructed using a TruSeq Nano DNA High Throughput Library Prep Kit (Illumina) ...
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bioRxiv - Microbiology 2021Quote: ... and 24 hours later cells were lysed with Passive Lysis 1x and reporter activities measured with Dual Luciferase Reporter Kit (Promega). At the same time supernatants from p1 cells were cleared by centrifugation and passaged onto fresh HEK293T-TIM1 p2 target cells ...
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bioRxiv - Biochemistry 2021Quote: i3Neurons were transfected with 2 μg of plasmid (SNAP-TM-Halo or Halo-TM-SNAP) per 25 mm coverslip using a calcium phosphate transfection kit (Promega), according to manufacturer’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... we measured the level of accumulated 2-deoxyglucose-6-phosphate (2DG6P) using the assay kit according to the manufacturer’s protocol using a GloMax®96 microplate luminometer (Promega).
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bioRxiv - Molecular Biology 2021Quote: ... Firefly luciferase activity was measured at 550 to 570 nm wavelength and followed by 100 μl of 1X Stop & Glo Reagent (part of the kit E1910, Promega) was added to each well ...
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bioRxiv - Genetics 2021Quote: ... all clippings from both hands and feet were used to extract genomic DNA with DNA IQ Casework Pro Kit (#AS1240, Promega) and Tissue and Hair Extraction Kit (#DC6740 ...
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bioRxiv - Cancer Biology 2021Quote: RNA was extracted from cells using the ReliaPrep™ RNA Miniprep System kit in accordance with the manufacturer’s protocol (Promega). RNA was reverse transcribed using the Applied Biosystems™ high capacity RNA to cDNA™ Kit in accordance with the manufacturer’s protocol ...