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Citations for Promega :
351 - 400 of 2358 citations for rno mir 32 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... cDNA synthesis was performed using random primers (Promega) and M-MLV reverse transcriptase (Promega) ...
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bioRxiv - Developmental Biology 2021Quote: ... plus 2 uL of random primers (Promega, C1181). This was then incubated at 70 °C for 10 mins ...
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bioRxiv - Immunology 2020Quote: ... and converted into cDNA using random primers (Promega) and M-MLV RT RNAse (H- ...
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bioRxiv - Molecular Biology 2023Quote: ... with random primers (Promega, Charbonnières-les-bains, France).
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bioRxiv - Immunology 2023Quote: ... dNTP Mix and Oligo (dT)15 Primer (Promega), Low Input Quick Amp Labeling Kit ...
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bioRxiv - Cell Biology 2024Quote: ... was used with random primers (Promega, Charbonniéres, France) to synthesize cDNA from 1μg of total RNA ...
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bioRxiv - Cancer Biology 2021Quote: MAGI2-AS3 or FOXN3 3’UTR containing miR-452-5p wild-type (WT) or mutant (MUT) binding sites were inserted into psiCHECK vector (Promega, USA). 293T cells were transfected with the constructed luciferase plasmid together with miR-NC or miR-452-5p mimics using Lipofectamine 2000 ...
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bioRxiv - Cancer Biology 2019Quote: A wild-type or mut-hsa-miR-329-5p fragment was constructed and inserted downstream of the luciferase reporter gene of the pmirGLO Vector (Promega, USA). We used ExFect 2000 Transfection Reagent to transfect the reporter plasmid into 293T cells ...
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bioRxiv - Cell Biology 2023Quote: ... qPCR reactions were set up with GoTaq® qPCR Master Mix (Promega) using diluted cDNA (5 ng/µl) ...
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bioRxiv - Genomics 2020Quote: ... TRs were amplified using the forward and reverse primers plus an M13 primer with one of the four fluorophores with GoTaq polymerase (Promega #PRM7123) using PCR program ...
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bioRxiv - Genomics 2023Quote: ... TRs were amplified using the forward and reverse primers plus an M13 primer with one of the four fluorophores with GoTaq polymerase (Promega #PRM7123) using PCR program ...
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bioRxiv - Microbiology 2020Quote: RT-qPCR analyses of NoV GII were carried out using GoTaq® Probe 1-Step RT-qPCR System (Promega, USA). Primers and the FAM/TAMRA-labelled probe of NoV GII were according to ISO 15216-1:2017 ...
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bioRxiv - Microbiology 2022Quote: RT-qPCR analyses of NoV GII were carried out using GoTaq® Probe 1-Step RT-qPCR System (Promega, USA). Primers and the FAM/TAMRA-labelled probe of hNoV GII were according to ISO 15216-1:2017 ...
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bioRxiv - Molecular Biology 2021Quote: ... Either GoTaq 1-Step RT-qPCR System (Promega) was used or reverse transcription for mRNAs was performed using Superscript IV RT (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: RNA was reverse transcribed using AMV RT (Promega) with random 9-mer primers ...
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bioRxiv - Neuroscience 2021Quote: ... and used for reverse transcription (Promega RT kit) to generate cDNA ...
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bioRxiv - Microbiology 2020Quote: ... RT was primed with oligo dT(15) (Promega) and held for 1 h at 42 °C ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5 μL of RT (4U, Promega®), followed by an incubation at 42°C for 60 minutes ...
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bioRxiv - Microbiology 2022Quote: ... and 0.25 μL of RT (4U, Promega®), followed by an incubation at 42°C for 60 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by reverse transcription with AMV RT (Promega) and RT primer ...
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bioRxiv - Genomics 2020Quote: ... 5 x RT Improm II reaction buffer (Promega), 50 ng hexanucleotides ...
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bioRxiv - Molecular Biology 2022Quote: ... GoTaq® qPCR and RT-qPCR Systems (Promega), and reverse transcription system (Promega) ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR using GoTaq qPCR Master Mix (Promega) was performed on a QuantStudio7 Flex (Applied Biosystems) ...
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bioRxiv - Biochemistry 2023Quote: ... and GoTaq 1-Step RT-qPCR System (Promega) were used for qPCR assays ...
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bioRxiv - Pathology 2023Quote: ... Complementary DNA was synthesized using MMLV-RT (Promega). The sequence of the primers for the Hamp ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was performed using AMV RT (Promega) in the presence of RNasin (Promega) ...
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bioRxiv - Evolutionary Biology 2023Quote: The 419 bp sequence of 3’UTR containing predicted target sites for miR-252 in CcTRPM was cloned into the pmirGLO vector (Promega, Wisconsin, USA) downstream of the luciferase gene to construct the recombinant plasmid of CcTRPM-3’UTR-pmirGLO using the pEASY®-Basic Seamless Cloning and Assembly Kit ...
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bioRxiv - Molecular Biology 2021Quote: ... reverse transcription assays were performed according to the manufacturer’s instructions with 2,5 μg of total RNA in the presence of 5 μM of oligo(dT) primer (Table S3) and 25 ng/μl of Random Primers (Promega Cat#C118A) using the SuperScript™ IV Reverse Transcriptase (Invitrogen Cat#18090010) ...
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bioRxiv - Cell Biology 2020Quote: ... 10 μl reactions were set up using GoTaq® Green Master Mix (Promega). Primers used to amplify zfccr5 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Reactions were set using the RiboMAX™ Large Scale RNA Production System (Promega) and incubated at 37ºC overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... qPCR reactions were set up with GoTaq qPCR Master Mix reagent system (Promega) and run on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... RNA samples and the negative extraction controls were screened in duplicate to investigate the presence of YFV RNA by RT-qPCR (GoTaq Probe 1-Step RT-qPCR System-Promega, USA), with primers and probes targeting the 5’-noncoding regions of the YFV genome (39) ...
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bioRxiv - Evolutionary Biology 2023Quote: Target gene and AAEL002401(reference gene) expression was quantified by RT-qPCR using FAM- and HEX -labelled GoTaq® Probe qPCR and RT-qPCR assay (Promega), TaqMan universal PCR master mix (Promega ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Levels of reporter mRNAs in eggs were measured 6 hrs post-injection by RT-qPCR using the GoTaq 2-Step RT-qPCR system as per manufacturer’s instructions (Promega, Madison, WI) with random primers for reverse transcription and oligos listed in Table 1 for the qPCR step.
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bioRxiv - Genetics 2021Quote: ... Colonies were genotyped using the T7/SP6 primers (Promega). Plasmids from the validated colonies were isolated using the QIAprep Spin Miniprep Kit (QIAGEN ...
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bioRxiv - Microbiology 2019Quote: ... along with 150 ng random primers (Promega, Madison, WI). Cycling conditions were as follows ...
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bioRxiv - Genomics 2021Quote: ... and Oligo (dT) 15 primer (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and Oligo (dT) 15 primer (Promega, Madison, WI, USA). STEP ONE PLUS Real Time PCR system (Applied Biosystems Japan ...
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bioRxiv - Plant Biology 2020Quote: ... the cDNA was synthesized using oligo(dT) primer (Promega) with 18S rRNA specific primer and ReverTraAce reverse transcriptase (Toyobo) ...
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bioRxiv - Bioengineering 2020Quote: ... 1 µl random primers (50 ng·µl−1, Promega, C1181), 2 µL 0.1M DTT ...
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bioRxiv - Microbiology 2021Quote: ... according to the manufacturers’ protocol with random primers (Promega). Two microliters of diluted cDNA were amplified with SYBR™ Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... random primers and RNasin® ribonuclease inhibitor (Promega N2511). All these steps were carried out following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis was performed using random primers (Promega, C1181) and M-MLV Reverse Transcriptase RNase H (Solis BioDyne ...
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bioRxiv - Developmental Biology 2019Quote: ... RNA was reverse transcribed using 250ng random primers (Promega), 0.5mM dNTPs (Promega) ...
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bioRxiv - Genetics 2020Quote: ... with both random hexamer and oligo(dT) primers (Promega) after the DNase (Promega ...
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bioRxiv - Immunology 2021Quote: ... 1µl of random hexamer primers (Promega, Catalogue no.-C118A) were added to the RNA ...
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bioRxiv - Plant Biology 2023Quote: ... oligo(dT) primer and reverse transcriptase ImProm-II (Promega). Two-step quantitative real-time PCR(qPCR ...
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bioRxiv - Bioengineering 2023Quote: ... 1 μL random primers (50 ng/μL, Promega, C1181), 2 μL 0.1 M DTT ...
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bioRxiv - Cell Biology 2020Quote: ... The partial frame of circ_0005962 containing the binding site or mutant binding site (wild-type or mutant-type) with miR-382-5p was amplified and cloned into the downstream of pGL4 vector (Promega, Madison, WI, USA) to generate circ_0005962-wt and circ_0005962-mut fusion plasmids ...
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bioRxiv - Cell Biology 2021Quote: ... of SNHG16 sequence and the 3’-untranslated region (UTR) fragment of STARD9 containing miR-1301-3p binding site were subcloned into the pmirGLO vectors (Promega, Madison, WI, USA) to generate SNHG16-Wt/Mut vectors and STARD9-Wt/Mut vectors ...