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Citations for Promega :
351 - 400 of 2350 citations for rno mir 125b 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... plus 2 uL of random primers (Promega, C1181). This was then incubated at 70 °C for 10 mins ...
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bioRxiv - Immunology 2020Quote: ... and converted into cDNA using random primers (Promega) and M-MLV RT RNAse (H- ...
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bioRxiv - Molecular Biology 2023Quote: ... with random primers (Promega, Charbonnières-les-bains, France).
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bioRxiv - Immunology 2023Quote: ... dNTP Mix and Oligo (dT)15 Primer (Promega), Low Input Quick Amp Labeling Kit ...
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bioRxiv - Cell Biology 2024Quote: ... was used with random primers (Promega, Charbonniéres, France) to synthesize cDNA from 1μg of total RNA ...
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bioRxiv - Cell Biology 2023Quote: ... qPCR reactions were set up with GoTaq® qPCR Master Mix (Promega) using diluted cDNA (5 ng/µl) ...
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bioRxiv - Genomics 2020Quote: ... TRs were amplified using the forward and reverse primers plus an M13 primer with one of the four fluorophores with GoTaq polymerase (Promega #PRM7123) using PCR program ...
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bioRxiv - Genomics 2023Quote: ... TRs were amplified using the forward and reverse primers plus an M13 primer with one of the four fluorophores with GoTaq polymerase (Promega #PRM7123) using PCR program ...
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bioRxiv - Microbiology 2020Quote: RT-qPCR analyses of NoV GII were carried out using GoTaq® Probe 1-Step RT-qPCR System (Promega, USA). Primers and the FAM/TAMRA-labelled probe of NoV GII were according to ISO 15216-1:2017 ...
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bioRxiv - Microbiology 2022Quote: RT-qPCR analyses of NoV GII were carried out using GoTaq® Probe 1-Step RT-qPCR System (Promega, USA). Primers and the FAM/TAMRA-labelled probe of hNoV GII were according to ISO 15216-1:2017 ...
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bioRxiv - Molecular Biology 2021Quote: ... Either GoTaq 1-Step RT-qPCR System (Promega) was used or reverse transcription for mRNAs was performed using Superscript IV RT (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: RNA was reverse transcribed using AMV RT (Promega) with random 9-mer primers ...
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bioRxiv - Neuroscience 2021Quote: ... and used for reverse transcription (Promega RT kit) to generate cDNA ...
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bioRxiv - Microbiology 2020Quote: ... RT was primed with oligo dT(15) (Promega) and held for 1 h at 42 °C ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5 μL of RT (4U, Promega®), followed by an incubation at 42°C for 60 minutes ...
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bioRxiv - Microbiology 2022Quote: ... and 0.25 μL of RT (4U, Promega®), followed by an incubation at 42°C for 60 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by reverse transcription with AMV RT (Promega) and RT primer ...
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bioRxiv - Genomics 2020Quote: ... 5 x RT Improm II reaction buffer (Promega), 50 ng hexanucleotides ...
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bioRxiv - Molecular Biology 2022Quote: ... GoTaq® qPCR and RT-qPCR Systems (Promega), and reverse transcription system (Promega) ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR using GoTaq qPCR Master Mix (Promega) was performed on a QuantStudio7 Flex (Applied Biosystems) ...
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bioRxiv - Biochemistry 2023Quote: ... and GoTaq 1-Step RT-qPCR System (Promega) were used for qPCR assays ...
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bioRxiv - Pathology 2023Quote: ... Complementary DNA was synthesized using MMLV-RT (Promega). The sequence of the primers for the Hamp ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was performed using AMV RT (Promega) in the presence of RNasin (Promega) ...
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bioRxiv - Evolutionary Biology 2023Quote: The 419 bp sequence of 3’UTR containing predicted target sites for miR-252 in CcTRPM was cloned into the pmirGLO vector (Promega, Wisconsin, USA) downstream of the luciferase gene to construct the recombinant plasmid of CcTRPM-3’UTR-pmirGLO using the pEASY®-Basic Seamless Cloning and Assembly Kit ...
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bioRxiv - Molecular Biology 2021Quote: ... reverse transcription assays were performed according to the manufacturer’s instructions with 2,5 μg of total RNA in the presence of 5 μM of oligo(dT) primer (Table S3) and 25 ng/μl of Random Primers (Promega Cat#C118A) using the SuperScript™ IV Reverse Transcriptase (Invitrogen Cat#18090010) ...
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bioRxiv - Cell Biology 2020Quote: ... 10 μl reactions were set up using GoTaq® Green Master Mix (Promega). Primers used to amplify zfccr5 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Reactions were set using the RiboMAX™ Large Scale RNA Production System (Promega) and incubated at 37ºC overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... qPCR reactions were set up with GoTaq qPCR Master Mix reagent system (Promega) and run on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... RNA samples and the negative extraction controls were screened in duplicate to investigate the presence of YFV RNA by RT-qPCR (GoTaq Probe 1-Step RT-qPCR System-Promega, USA), with primers and probes targeting the 5’-noncoding regions of the YFV genome (39) ...
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bioRxiv - Evolutionary Biology 2023Quote: Target gene and AAEL002401(reference gene) expression was quantified by RT-qPCR using FAM- and HEX -labelled GoTaq® Probe qPCR and RT-qPCR assay (Promega), TaqMan universal PCR master mix (Promega ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Levels of reporter mRNAs in eggs were measured 6 hrs post-injection by RT-qPCR using the GoTaq 2-Step RT-qPCR system as per manufacturer’s instructions (Promega, Madison, WI) with random primers for reverse transcription and oligos listed in Table 1 for the qPCR step.
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bioRxiv - Genetics 2021Quote: ... Colonies were genotyped using the T7/SP6 primers (Promega). Plasmids from the validated colonies were isolated using the QIAprep Spin Miniprep Kit (QIAGEN ...
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bioRxiv - Microbiology 2019Quote: ... along with 150 ng random primers (Promega, Madison, WI). Cycling conditions were as follows ...
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bioRxiv - Genomics 2021Quote: ... and Oligo (dT) 15 primer (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and Oligo (dT) 15 primer (Promega, Madison, WI, USA). STEP ONE PLUS Real Time PCR system (Applied Biosystems Japan ...
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bioRxiv - Plant Biology 2020Quote: ... the cDNA was synthesized using oligo(dT) primer (Promega) with 18S rRNA specific primer and ReverTraAce reverse transcriptase (Toyobo) ...
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bioRxiv - Bioengineering 2020Quote: ... 1 µl random primers (50 ng·µl−1, Promega, C1181), 2 µL 0.1M DTT ...
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bioRxiv - Microbiology 2021Quote: ... according to the manufacturers’ protocol with random primers (Promega). Two microliters of diluted cDNA were amplified with SYBR™ Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... random primers and RNasin® ribonuclease inhibitor (Promega N2511). All these steps were carried out following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis was performed using random primers (Promega, C1181) and M-MLV Reverse Transcriptase RNase H (Solis BioDyne ...
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bioRxiv - Developmental Biology 2019Quote: ... RNA was reverse transcribed using 250ng random primers (Promega), 0.5mM dNTPs (Promega) ...
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bioRxiv - Genetics 2020Quote: ... with both random hexamer and oligo(dT) primers (Promega) after the DNase (Promega ...
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bioRxiv - Immunology 2021Quote: ... 1µl of random hexamer primers (Promega, Catalogue no.-C118A) were added to the RNA ...
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bioRxiv - Plant Biology 2023Quote: ... oligo(dT) primer and reverse transcriptase ImProm-II (Promega). Two-step quantitative real-time PCR(qPCR ...
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bioRxiv - Bioengineering 2023Quote: ... 1 μL random primers (50 ng/μL, Promega, C1181), 2 μL 0.1 M DTT ...
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bioRxiv - Cell Biology 2021Quote: ... of SNHG16 sequence and the 3’-untranslated region (UTR) fragment of STARD9 containing miR-1301-3p binding site were subcloned into the pmirGLO vectors (Promega, Madison, WI, USA) to generate SNHG16-Wt/Mut vectors and STARD9-Wt/Mut vectors ...
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bioRxiv - Cell Biology 2021Quote: ... and 1-3μl aliquots were taken for reverse transcription (RT) using the Moloney murine leukemia virus RT RNase H (−) (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: The quantification of viral RNA was carried out in one-step RT-qPCR using 3 µl of the eluted RNA with the GoTaq® Probe One-step RT-qPCR System (Promega, Poland). This procedure employed in-house primers and a probe specifically tailored for the HCoV-229E N sequence ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was synthesized using M-MLV RT (H–) (Promega) and U3 antisense primer 5’-TGTGTGGTAGATCCACATCG-3’ ...
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bioRxiv - Plant Biology 2021Quote: ... 0.5 µL reverse transcriptase (RT, Promega, Madison, Wisconsin, US) and RNase-free water were added ...