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Citations for Promega :
351 - 400 of 3337 citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 50 μL of CellTiter-Glo reagent (Promega, G7572) was added to each well and incubated for 10 min at room temperature after 6 h of incubation at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µL Luciferin Generation Reagent (Promega Cat #TM344) was added to all wells ...
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bioRxiv - Developmental Biology 2023Quote: ... 50 μl suspended streptavidin MagneSphere beads (Promega #Z5481) per sample were washed 3×5 minutes in 0.5x SSC ...
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bioRxiv - Microbiology 2024Quote: ... and 50 µl of SteadyGlo luciferase reagent (Promega) was added to each well ...
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bioRxiv - Microbiology 2024Quote: ... and 50 µl NanoLuc working solution (NanoGlo, Promega) was added to the wells ...
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bioRxiv - Biophysics 2024Quote: ... 50 ng pGlo-22F plasmid (Promega, Madison, WI), and 500 ng polyethyleneimine in 25 μL Opti-mem (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... 50 μL of Kinase-Glo Max reagent (Promega) was added to each well ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... MBP-LIC1 (50 pmol) and either BICD2N-Halo (50 pmol) or CBD-HAP1CC1 (100 pmol) was incubated with resin (Magne® HaloTag, Promega, G7281 ...
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bioRxiv - Biophysics 2023Quote: DNA dT70 was diluted to ∼100 pM in buffer (50 mM Tris-acetate pH 7.7, 5 mM Mg-acetate, 50 mM Na-acetate) and SSB (Promega Corporation, USA) was supplemented in specified concentrations (Tab ...
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bioRxiv - Cell Biology 2023Quote: Activity of Hsf1 was determined by adding 50 µl of cell suspension (cells expressing the pSSA3HSE-lacZ plasmid) to 50 µl of β-Glo reagent (Promega) in a white 96-well plate ...
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bioRxiv - Cancer Biology 2023Quote: ... at 3,000 cells/well in 50 μL media containing IFN-α for 72 h prior to cell lysis with 50 μL CellTiterGlo 2.0 (Promega, G9243). The reaction was incubated in the dark for 20 min at RT ...
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bioRxiv - Genetics 2024Quote: ... cells were washed twice in PBS and incubated for 5 min at room temperature with 50 µL of a 50 µg/mL RNAse A solution (Promega A7973). Finally ...
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bioRxiv - Systems Biology 2024Quote: ... the digestion was performed overnight in 50 µL of 50 mM of TEAB containing sequencing grade trypsin (Promega, Madison, WI, USA) in ratio 1:40 (w/w ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was then incubated at 37°C for overnight trypsin digestion (trypsin 1:50 protein w/w ratio; Promega, Madison, WI). The digestion reaction was quenched by adding formic acid (FA ...
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bioRxiv - Biochemistry 2020Quote: ... Trypsinisation was carried out by incubating the gel pieces overnight at 37 °C in 50 µl of trypsin (1: 25:: trypsin: protein, 20 µg/ml, sequencing grade trypsin V5111, Promega, Madison, WI) prepared in ammonium bicarbonate (50 mM) ...
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bioRxiv - Microbiology 2020Quote: ... Positive clones were cultured in liquid LB medium supplemented with kanamycin (50 μg mL−1) for extraction of plasmid DNA with the Wizard Plus SV Minipreps DNA Purification System Kit (Promega - Madison, USA). Plasmids with unique EcoRI/HindIII restriction patterns were subjected to re-transformation and phenotypic confirmation by streaking the clones in solid LB medium supplemented with kanamycin (50 μg mL−1) ...
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bioRxiv - Neuroscience 2022Quote: ... we transferred the beads to 100 µL 50 mM Tris-HCl buffer with mixed with 1 µg trypsin/Lys-C (Promega Cat# V5073) per replicate ...
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bioRxiv - Cell Biology 2020Quote: ... Columns were washed with 90% methanol/100 mM TEAB 10 times prior to on-column digestion with 1 ug Trypsin/Lys-C in 50 mM TEAB (47° C, 2 hours; Cat. #V5073, Promega, Madison, WI). The resulting peptides were eluted with sequential washes of 50 mM TEAB ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were precipitated with isopropanol50 and digested overnight at 37°C with 1:50 enzyme : protein ratio by Trypsin/Lys-C Mix (Promega GmbH, Germany). Resulting peptides were desalted on MicroSpin column (The Nest Group ...
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bioRxiv - Physiology 2022Quote: ... protein samples were digested by incubated overnight at 37°C with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin). Samples were acidified using 5% TFA and peptides cleaned up using the Phoenix 96× kit (PreOmics ...
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bioRxiv - Neuroscience 2022Quote: ... Protein digestion was processed for overnight by adding LysC and trypsin in a 1:50 ratio (µg of the enzyme to µg of protein; Promega; Cat#: V5071)) at 37° C and 1400 rpm ...
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bioRxiv - Immunology 2024Quote: ... The reaction was stopped by addition of 50 µl/well of 1 M HCl and absorbance read at 450 nm using a GloMax microplate reader (Promega, Dübendorf, Switzerland).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Bound proteins were eluted with elution buffer 1 (50 mM Tris-HCl pH 7.5, 2 M urea, 5 ng/µL sequencing grade modified trypsin (Promega, Madison, WI, USA), 1 mM DTT ...
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bioRxiv - Immunology 2022Quote: ... the buffer was diluted with 50 mM ABC to a final of 2M urea and digested overnight at RT with 1 µg trypsin (Promega, Walldorf, Germany). On the next day ...
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bioRxiv - Biophysics 2023Quote: ... They were then diluted 5-fold with aqueous 50 mM TEAB and incubated overnight with Sequencing Grade Modified Trypsin (1 ug in 10 uL of 50 mM TEAB; Promega, Madison, WI). Following this an equal volume of ethyl acetate/trifluoroacetic acid (TFA ...
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bioRxiv - Biochemistry 2023Quote: ... The captured proteins were washed twice in 200 μl of 95% MeCN and transferred to 200 μl of 50 mM ammonium bicarbonate (ABC) containing 1 μg sequencing grade modified trypsin (Promega, Madison, USA) and mixed for 2 hr at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... 500 ng of vector was mixed with 50 uL of Opti-MEM and 1 μL of FuGENE HD Transfection Reagent (Promega; Table S2). This mixture was incubated at room temperature for 20 min before adding dropwise to the well ...
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Ethanolamine-induced assembly of microcompartments is required for Fusobacterium nucleatum virulencebioRxiv - Microbiology 2024Quote: ... They were then diluted 5-fold with aqueous 50 mM TEAB and incubated overnight with Sequencing Grade Modified Trypsin (1 µg in 10 µl of 50 mM TEAB; Promega, Madison, WI) following which an equal volume of ethyl acetate/trifluoroacetic acid (TFA ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were initially digested for 1 hour at 47°C with sequencing grade trypsin (Promega, San Luis Obispo, CA) dissolved in 50 mM TEAB (pH 7 ...
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bioRxiv - Neuroscience 2020Quote: ... samples were digested with 50 μl of 50 mM ammonium bicarbonate solution containing 5 ng/μl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 50 µL supernatant were transferred to a white 96-well plate and mixed with 50 µL of CellTiter-Glo (Promega, Dübendorf, Switzerland). Measurements were performed on a HIDEX Sense microplate reader and viability was set relative to the respective DMSO control ...
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bioRxiv - Microbiology 2020Quote: ... 50 µ L of Kinase-Glo® reagent (Promega) was then added and incubated at room temperature for 10 minutes ...
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bioRxiv - Genomics 2020Quote: ... and 50 ng pRL-SV40 Renilla luciferase vector (Promega), using the Lipofectamine LTX reagent ...
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bioRxiv - Microbiology 2020Quote: ... 50 bp ladder was from Promega (catalogue No. G4521).
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bioRxiv - Molecular Biology 2020Quote: ... and 50 μl of Bright-Glo luciferase substrate (Promega) was added to each well.
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bioRxiv - Evolutionary Biology 2022Quote: ... 50 μL of luciferase substrate (Promega, Shanghai China, N1120) was added to each well ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 50 ng of pGL3 firefly luciferase expression vector (Promega) using GenJet (Signagen ...
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bioRxiv - Developmental Biology 2020Quote: ... 150 μl 50 mM TEAB + Sequencing Grade Trypsin (Promega) was added and left overnight at 38° C ...
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bioRxiv - Immunology 2021Quote: ... 50 μL/well of One-Glo-EX substrate (Promega) were added to the cells and incubated in the dark for 5-10 min prior reading on a Varioskan LUX plate reader (ThermoFisher) ...
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Evolutionary plasticity of SH3 domain binding by Nef proteins of the HIV-1/SIVcpz lentiviral lineagebioRxiv - Microbiology 2021Quote: ... plus 50 ng of the plasmid pRL-TK (Promega) expressing low and constitutive levels of Renilla luciferase ...
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bioRxiv - Molecular Biology 2023Quote: ... Then 50 µl of digestion buffer with trypsin (Promega) was added at an enzyme-to-substrate ratio of 1:100 ...
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bioRxiv - Cell Biology 2024Quote: ... 50 µL of CellTiter Glo reagent (Promega, Madison, WI) was added to each well ...
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bioRxiv - Cell Biology 2024Quote: ... a solution containing 50 nM JF549-HTL (Promega GA1110) was applied for 15 min at 37°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 50 mM TEAB and 2 µg LysC+trypsin (Promega) were added ...
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bioRxiv - Neuroscience 2024Quote: ... and 50 U/ml of RNasin (Promega, cat# N2611). Finally ...
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bioRxiv - Bioengineering 2023Quote: ... 50 ng trypsin (Sequencing Grade Modified Trypsin, Lyophilized, Promega) was added to the hydrogels and incubated for 30 min to permeate the hydrogels at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µL Total Glutathione Lysis Reagent (Promega Cat #TM344) was added to 3 of the wells per treatment condition and 50 µL Oxidized Glutathione Lysis Reagent (Promega Cat #TM344 ...
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bioRxiv - Neuroscience 2022Quote: ... 150 μl 50 mM TEAB + Sequencing Grade Trypsin (Promega) was added and left overnight at 38° C ...
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bioRxiv - Microbiology 2024Quote: ... mixed with 50 µL reconstituted luciferase assay buffer (Promega), and luciferase measured immediately on a plate reader (Biotek Synergy H1) ...
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bioRxiv - Immunology 2024Quote: ... 50 μl of Bright-Glo Luciferase reagent (Promega E2620) was added to each well ...